-
No products found
because this supplier's products are not listed.
Michael J Massare, et al.,
bioRxiv - Immunology 2021
Quote:
... pBac1 plasmids were transfected into Sf9 with Flash-bacGOLD bacmid containing the Autographa californica polydedrosis virus genome (Oxford Expression Technology, Oxford UK). Sf9 cells cultures were infected with recombinant baculovirus expressing the HA genes ...
-
No products found
because this supplier's products are not listed.
Ria Lassaunière, et al.,
bioRxiv - Immunology 2023
Quote:
This study is part of national infectious disease surveillance and response to an emerging virus variant performed on surplus biological material by Statens Serum Institut, a governmental institute under the Danish Ministry of Health ...
-
No products found
because this supplier's products are not listed.
Sabina Marciano, et al.,
bioRxiv - Neuroscience 2021
Quote:
... primary antibody incubation was performed for 24 h at room temperature (VMAT2: 1:5000, 20042, Immunostar, Hudson, USA, HA-tag: 1:5000, MMS- 101R, Nordic BioSite, Taby ...
-
No products found
because this supplier's products are not listed.
Kejia Zhang, et al.,
bioRxiv - Molecular Biology 2024
Quote:
... FLAG-tagged proteins were purified by incubating whole cell lysates from the transfected cell lines with 20 μL of DYKDDDDK-Tag Monoclonal Antibody Magnetic Microbead (Syd Labs) for three hours at 4 °C ...
-
No products found
because this supplier's products are not listed.
Leslie Duplaquet, et al.,
bioRxiv - Cancer Biology 2024
Quote:
The following chemicals (stored at −20°C or −80°C) were added to cell culture where indicated: BVdU was purchased from Chem-Impex International Inc. ...
-
No products found
because this supplier's products are not listed.
M. A. Rossotti, et al.,
bioRxiv - Bioengineering 2021
Quote:
... three-fold dilutions of VHH-Fcs were prepared in V- Bottom 96-well microtiter plates (Globe Scientific, Mahwah, NJ, Cat# 120130) and mixed with 50 µL of CHO-SPK cells ...
-
No products found
because this supplier's products are not listed.
Bettina M. Fuglerud, et al.,
bioRxiv - Genomics 2021
Quote:
... at 4 °C overnight in CHAPS immunoprecipitation buffer (Fivephoton Biochemicals). After washing in TBST ...
-
No products found
because this supplier's products are not listed.
Shalini Gupta, et al.,
bioRxiv - Molecular Biology 2021
Quote:
... The N- and C-peptides were labeled with maleimide-derivatized DY549P1 (Dyomics), DY649P1 (Dyomics) ...
-
No products found
because this supplier's products are not listed.
Christian S. Stevens, et al.,
bioRxiv - Microbiology 2022
Quote:
... and fluorescent probe (5’-6FAM-ACAGACGTTGTATA+C+CAT+G-TMR) (TIB MOLBIOL) RT-qPCR was performed using the following cycle ...
-
No products found
because this supplier's products are not listed.
Erika Riederer, et al.,
bioRxiv - Biophysics 2021
Quote:
... 1 µM valinomycin at 30 °C using 100 nM of 14C Asp (Moravek Biochemicals) as previously described.(33 ...
-
No products found
because this supplier's products are not listed.
Matthew J. Foulkes, et al.,
bioRxiv - Immunology 2019
Quote:
... The c-Jun N-terminal kinase inhibitor SP600125 was obtained from StressMarq Biosciences (Victoria, Canada), and tanshinone IIA (TIIA ...
-
No products found
because this supplier's products are not listed.
Lindsay Smith, et al.,
bioRxiv - Developmental Biology 2020
Quote:
... zebrafish were incubated for 5 min at 28.5°C with optovin 6b8 (ID 5705191l; ChemBridge), an optovin analog (Kokel et al. ...
-
No products found
because this supplier's products are not listed.
Adrian S. Monthony, et al.,
bioRxiv - Plant Biology 2020
Quote:
... The callus induction medium (hereafter referred to as LT-C) consisted of MS (M524; Phytotechnology Laboratories) nutrients ...
-
No products found
because this supplier's products are not listed.
Toshiyuki Oda, et al.,
bioRxiv - Immunology 2022
Quote:
... and 4 µl of HMC buffer containing cytochrome c-stabilized 15-nm colloidal gold (BBI Solutions) was applied for attachment of fiducial markers (Oda et al. ...
-
No products found
because this supplier's products are not listed.
Genjiro Suzuki, et al.,
bioRxiv - Neuroscience 2020
Quote:
... for 60 minutes at 37°C or 50 μM fluorogenic substrate (boc-LRR-mca, Peptide Institute) for 10 minutes at 37°C ...
-
No products found
because this supplier's products are not listed.
Kelsey M. Tyssowski, Jesse M. Gray,
bioRxiv - Neuroscience 2019
Quote:
... The temperature was maintained by putting the plate on a 37°C warming plate (Bel-Art). The CO2 was maintained at 5% throughout the duration of the recording using the base provided with the Axion Lumos system ...
-
No products found
because this supplier's products are not listed.
Tamás Bakos, et al.,
bioRxiv - Immunology 2024
Quote:
... Soluble terminal C complex (sTCC, sC5b9) was measured with an ELISA kit from Svar Life Science AB (Malmö ...
-
No products found
because this supplier's products are not listed.
Doris Krauter, et al.,
bioRxiv - Neuroscience 2021
Quote:
... Western Blots were incubated overnight at 4 °C in primary antibodies against PMP22 (1:1000, Assay Biotech), PTEN (1:1000 ...
-
No products found
because this supplier's products are not listed.
Tomoki Togashi, et al.,
bioRxiv - Bioengineering 2023
Quote:
... hPC antigen (hPC:Ag) was measured using the Human Protein C AssayMaxTM ELISA Kit (Assaypro, St. Charles, MO) according to the manufacturer’s instructions ...
-
No products found
because this supplier's products are not listed.
Vera Kovaleva, et al.,
bioRxiv - Cell Biology 2020
Quote:
... Cells were incubated overnight at +4°C with the following primary antibodies: anti-MANF rabbit pAb (Icosagen, 310-100), anti-IRE1α rabbit mAb (CST ...
-
No products found
because this supplier's products are not listed.
Tobias Butelmann, et al.,
bioRxiv - Bioengineering 2021
Quote:
... an LM was taken out from the medium (30 °C) and weighed on a precision scale (Adam Equipment, UK). To determine the dry weight of the cells ...
-
No products found
because this supplier's products are not listed.
Alex Quintero-Yanes, Aurélie Mayard, Régis Hallez,
bioRxiv - Microbiology 2022
Quote:
... Temperature (30 °C) was maintained stable during microscopy analysis using the Tempcontrol 37-analog 1 channel equipment (HemoGenix®) coupled to the Axio Imager Z1 microscope ...
-
No products found
because this supplier's products are not listed.
Yurika Matsui, et al.,
bioRxiv - Developmental Biology 2022
Quote:
... cells were incubated at 37 °C with reconstituted FAM-FLICA® at a 1:300 dilution (ImmunoChemistry Technologies 94) for 30 min ...
-
No products found
because this supplier's products are not listed.
Zahra Hosseinzadeh, et al.,
bioRxiv - Bioengineering 2023
Quote:
... The insulin of each sample was measured with the C-Peptide & Insulin AccuBind VAST ELISA Kits (Monobind Inc., USA). Three independent experiments were performed for each group ...
-
No products found
because this supplier's products are not listed.
Peter A. van der Ley, et al.,
bioRxiv - Immunology 2021
Quote:
The prefusion stabilized HexaPro spike protein27 with and without a 3xGGGS spacer and mCRAMP sequence added to the C-terminus were produced by Celonic AG (Basel ...
-
No products found
because this supplier's products are not listed.
Brian C. Russo, et al.,
bioRxiv - Microbiology 2021
Quote:
... The cells were then incubated overnight at 4°C with rabbit anti-Shigella conjugated to FITC (ViroStat, catalog no. 0903). The next day ...
-
No products found
because this supplier's products are not listed.
Guizhen Fan, et al.,
bioRxiv - Biophysics 2022
Quote:
... Membranes were probed with a rabbit polyclonal antibody against the C-terminal 19aa of IP3R1 (CT1; #ARC154, Antibody Research Corporation) at a 1:1000 dilution ...
-
No products found
because this supplier's products are not listed.
Jugal Mohapatra, et al.,
bioRxiv - Biochemistry 2021
Quote:
... and heated to 90 °C for 2 min while bubbling with nitrogen gas in N,N-dimethylformamide (DMF, Oakwood Chemical). Fmoc deprotection was carried out with 20% piperidine (Sigma-Aldrich ...
-
No products found
because this supplier's products are not listed.
William J Dower, et al.,
bioRxiv - Immunology 2023
Quote:
... for 5-10 min at 25°C and the reaction was stopped using STOP solution (Surmodics Cat# LSTP-1000-01).
-
No products found
because this supplier's products are not listed.
Alyssa F. Pybus, et al.,
bioRxiv - Neuroscience 2023
Quote:
... Samples were incubated at 4°C overnight with primary antibodies diluted in blocking buffer: GFAP (1:100, Diagnostic Biosystems Mob064), NeuN (1:200 ...
-
No products found
because this supplier's products are not listed.
Juan A. Perez-Bermejo, et al.,
bioRxiv - Molecular Biology 2023
Quote:
At least 5×107-3×108 Cryopreserved CD34+ HSPCs were then thawed at 37 °C and cultured in supplemented cytokine rich SCGM media (CellGenix) containing recombinant cytokines at 100 ng/mL each Flt-3L ...
-
No products found
because this supplier's products are not listed.
Samuel Schmidt, et al.,
bioRxiv - Cell Biology 2020
Quote:
... incubated 24h at 4 °C) were used as substrates and prepared in Willco dishes (35 mm, Willco Wells B.V., HBSt-3522) as described46 ...
-
No products found
because this supplier's products are not listed.
Genevieve. McCluskey, et al.,
bioRxiv - Immunology 2021
Quote:
The effect of FH on protein C (PC) activation was monitored by combining FH (0.5-125nM) with 1.75nM thrombin and 200nM PC (Haematologic Technologies, Inc., USA) in the absence or presence of 10nM soluble recombinant TM (R&D Systems/Biotechne ...
-
No products found
because this supplier's products are not listed.
Anna Vilalta, et al.,
bioRxiv - Biochemistry 2020
Quote:
... Proteins in storage buffer were concentrated to between 5-10 mg/ml for use and tested for endotoxin with the EndoZyme recombinant Factor C assay (Hyglos GmbH). Endotoxin levels in all assays were maintained at < 0.1 EU/ml.
-
No products found
because this supplier's products are not listed.
Bruno Motta Nascimento, Nikhil Unni Nair,
bioRxiv - Bioengineering 2020
Quote:
... coli were hydrolyzed 6 M HCl at 100 °C for 4 h in a vacuum sealed tube (Chemglass, #CG-4025-01). Acid was immediately removed with a vacuum centrifuge and pellet was resuspended in deionized water ...
-
No products found
because this supplier's products are not listed.
Quentin Sabbagh, et al.,
bioRxiv - Cancer Biology 2021
Quote:
... 4°C) by size exclusion chromatography (SEC) using 70 nm Original qEV columns associated with an automatic fraction collector (Izon Science), according to the manufacturer’s protocol and recommendations from the International Society for Extracellular Vesicles (ISEV ...
-
No products found
because this supplier's products are not listed.
Henriette Frikke-Schmidt, et al.,
bioRxiv - Physiology 2020
Quote:
... The plates for the islets were placed on a mat heated at 37 °C on a H101A ProScan motorized stage (Prior Scientific Instruments Ltd ...
-
No products found
because this supplier's products are not listed.
A Elgheznawy, et al.,
bioRxiv - Biochemistry 2021
Quote:
... Platelets were activated with appropriately diluted agonists for 8 min at 37°C followed by 8 min at RT in the presence of saturating amounts of PE-coupled JON/A (4H5, Emfret Analytics) detecting activated αIIbβ3 integrin and FITC-coupled anti-P-selectin (Wug.E9 ...
-
No products found
because this supplier's products are not listed.
Alan P. R. Lorenzetti, et al.,
bioRxiv - Systems Biology 2022
Quote:
... Cell pellets were resuspended in Milli-Q water and disrupted at 4°C using ceramic beads (Mo Bio Laboratories) and a Precellys 24 homogenizer (Bertin Corp). Protein content was determined by bicinchoninic acid assay (BCA ...
-
No products found
because this supplier's products are not listed.
W. Coles Keeter, et al.,
bioRxiv - Immunology 2023
Quote:
... 8-12-week-old male mice were placed on a high fat diet with 0.15% added cholesterol (HFD-C, fat: 60% kcal; carbohydrate: 26% kcal, Bio-Serv F3282) for 28 weeks until sacrifice.
-
No products found
because this supplier's products are not listed.
Cameron Durfee, et al.,
bioRxiv - Cancer Biology 2023
Quote:
... They were then baked at 65°C for 20 minutes to deparaffinize and then rehydrated with three consecutive washes in CitriSolv (Decon Labs) for 5 min each followed by graded alcohols as above ...
-
No products found
Tao Cheng, et al.,
bioRxiv - Developmental Biology 2023
Quote:
... 15 minutes at 4°C) from blood isolated by sub-mandibular bleeding and used for the BUN assay (BUN-Urea, BioAssay Systems) following the manufacturer’s protocol.
-
No products found
because this supplier's products are not listed.
Stijn Mouton, et al.,
bioRxiv - Developmental Biology 2023
Quote:
... the overnight cultures were used both to create glycerol stocks for long-term storage at -80°C and for plasmid DNA extractions using the Plasmid DNA Extraction Mini Kit (Favorgen®). The extracted plasmids were used for Sanger sequencing using a universal T444T forward sequencing primer (Table S1).
-
No products found
because this supplier's products are not listed.
Hui Zhang, et al.,
bioRxiv - Developmental Biology 2023
Quote:
... Samples were boiled at 100 °C in a metal bath for 10 min in protein loading buffer (CoWin Biosciences, Beijing, China) and equal amount proteins were separated by 10% SDS-PAGE gel ...
-
No products found
because this supplier's products are not listed.
Lauren Rylaarsdam, et al.,
bioRxiv - Neuroscience 2023
Quote:
... Organoid pieces were incubated for 75 minutes on a 95 RPM orbital shaker in a 37°C incubator and triturated using wide bore tips (Rainin, 30389218) every 30 minutes to help break up pieces ...
-
No products found
because this supplier's products are not listed.
Grigorios Fanourgakis, et al.,
bioRxiv - Genetics 2024
Quote:
... After washing cells were incubated with 20 μg/ml with Hoechst 33342 (Thermo Fischer Scientific H3570) for 1 hour at 32°C with constant shaking and protected from light and then with 30nM DRAQ7 (Biostatus DR71000) for 5 minutes on bench ...
-
No products found
because this supplier's products are not listed.
Philippe Dehio, et al.,
bioRxiv - Cell Biology 2024
Quote:
... The slides were first plasma cleaned and PEG-coated at 4 °C overnight with 200µg/ml poly(L-lysine)-PEG (SuSoS, PLL(20)-g[3.5]-PEG(2) ...
-
No products found
because this supplier's products are not listed.
Chloe C. Ence, et al.,
bioRxiv - Microbiology 2024
Quote:
... The cultures were incubated at 37°C with 5% CO2 and routinely checked for mycoplasma contamination using the e-Myco Plus kit from Intron Biotechnology. Human hepatocellular carcinoma cells (HepG2 ...
-
No products found
because this supplier's products are not listed.
Claudia Ctortecka, et al.,
bioRxiv - Biochemistry 2024
Quote:
... the proteoCHIP EVO 96 is inserted into the cellenONE® using a dedicated holder (C-PEVO-96-CHB, Scienion, Berlin, Germany), 150nL of master mix (0.2% DDM (D4641-500MG ...
-
No products found
because this supplier's products are not listed.
Tongcui Ma, et al.,
bioRxiv - Microbiology 2023
Quote:
... or conjugated in-house with X8 antibody-labeling kits (Standard BioTools) and stored at 4°C in Antibody Stabilizer (Boca Scientific) supplemented with 0.05% sodium azide ...