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Citations for Vector Labs :
1 - 50 of 139 citations for Crimean Congo Haemorrhagic Fever virus CCHFV glycoprotein C Gc sheep Fc tag since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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Dissecting β-Cardiac Myosin and Cardiac Myosin-Binding Protein C Interactions using a Nanosurf AssaybioRxiv - Biophysics 2022Quote: ... construct with a C-terminal eGFP tag and N-terminal FLAG tag was generated and subcloned into a shuttle vector provided by Vector Laboratories, Burlingame ...
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bioRxiv - Immunology 2022Quote: ... The column was washed with PBS and the bound glycoproteins were eluted with glycoprotein eluting solution for mannose/glucose binding lectins (Vector Labs: ES-1100). The glycoproteins were dialyzed in PBS ...
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bioRxiv - Neuroscience 2022Quote: ... Biotin-conjugated polyclonal anti-sheep IgG (Vector Labs) in the case of anti Sema3F antibody was applied for 1 h at RT ...
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bioRxiv - Biophysics 2021Quote: ... Digoxigenin-labelled probes were detected using one layer of rhodamine-conjugated sheep anti-digoxigenin and a second layer of Texas red–conjugated anti-sheep (Vector Laboratories). Biotinlabelled probes were detected using one layer of FITC-conjugated streptavidin followed by a layer of biotin-conjugated anti-avidin and a second layer of FITC-conjugated streptavidin (Vector Laboratories) ...
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bioRxiv - Biochemistry 2021Quote: ... Glycoproteins were probed with 1.7 μg/ml biotin-conjugated ricin (RCA-120, Vector Laboratories, UK) in blocking buffer before or after pre-incubation with 10 mg/ml D-galactose and 10 mg/ml lactose to confirm specific ricin binding ...
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bioRxiv - Molecular Biology 2024Quote: ... glycoproteins were probed for 1 hour using biotinylated-TL (1:5000 dilution, Vector Laboratories Inc.) in 3% BSA blocking buffer ...
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bioRxiv - Molecular Biology 2024Quote: ... Glycoproteins were probed with 1.7 μg/mL biotin-conjugated RCA (RCA-120, Vector Laboratories, UK) in blocking buffer before or after pre-incubation with 30 mg/mL D-galactose and 30 mg/mL lactose to confirm specific RCA binding ...
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bioRxiv - Microbiology 2023Quote: ... SOSIP glycoproteins were eluted with 1M methyl-α-D-mannopyranoside (from Vector Laboratories or MilliporeSigma), filtered through 0.2 μm filter and concentrated using Vivaspin 6 centrifugal concentrators (30kDa ...
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bioRxiv - Molecular Biology 2022Quote: ... a biotinylated anti-sheep IgG or anti-rabbit IgG (Vector Laboratories) were used ...
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bioRxiv - Cell Biology 2020Quote: ... followed by Texas Red α-sheep antibody (Vector Labs, 1:100).
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bioRxiv - Molecular Biology 2021Quote: ... and rabbit anti-sheep FITC antibodies (FI-6000, Vector Laboratories, RRID: AB_2336218). Widefield fluorescence imaging was performed on a DeltaVision Elite system (Applied Precision ...
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bioRxiv - Genetics 2019Quote: ... followed by rabbit anti–sheep FITC 1/100 (Vector Laboratories, FI-6000). Coverslips were stained with DAPI (0.5 μg/mL in PBS) ...
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bioRxiv - Molecular Biology 2021Quote: ... and fluorescein rabbit anti-sheep antibodies (1:100, Vector Labs FI-6000); biotin probes were detected using streptavidin-555 (1:1000 ...
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bioRxiv - Microbiology 2021Quote: ... Elution was done in 4 bed volumes with corresponding elution buffers (Glycoprotein Eluting Solution, Cat. No. ES-3100 Vector labs for AAL column and 0.1M Melibiose monohydrate for GSI Cat# AK-2401-2) ...
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bioRxiv - Cancer Biology 2021Quote: ... Fucosylated proteins were eluted twice with 50 uL AAL elution buffer B1 or 40 uL glycoprotein eluting solution for fucose-binding lectins (Vector Laboratories) at 4°C for 1 h per round ...
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bioRxiv - Microbiology 2021Quote: ... Infected cells were visualized by labeling with anti-flavivirus E-glycoprotein antibody using a mouse VECTASTAIN ABC-horseradish peroxidase (HRP) kit (PK-4002; Vector Laboratories) and a DAB peroxidase substrate kit (SK-4100 ...
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bioRxiv - Immunology 2020Quote: ... Proteins without His tags were purified with GNL columns (Vector Labs). The bound proteins were washed with PBS and then eluted with 50 mL of 1M Methyl α-D-mannopyranoside (Sigma M6882-500G ...
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bioRxiv - Neuroscience 2020Quote: ... Sections were again rinsed in PB and then incubated overnight in biotinylated donkey anti-sheep IgG in PB (1:200, Vector Labs), followed by overnight incubation in streptavidin-conjugated Alexa 488 in PB (1:800 ...
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bioRxiv - Biochemistry 2024Quote: ... and RBD (lacking an LPETG tag) were biotinylated using the ChromaLINK biotinylation kit (Vector labs). Manufacturer’s instructions were used ...
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bioRxiv - Physiology 2019Quote: ... Tissue slides were then acetone-fixed followed by incubation with Fc-receptor blocking in 2.5% goat serum (Vector Laboratories) and incubation with primary antibodies cocktail containing anti-CD11b:AlexaFluor488 and CD11c:PE (Biolegend) ...
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bioRxiv - Neuroscience 2021Quote: ... Sections were incubated with primary antibody for 16-24 hrs at 4°C and then for 2 hrs at 4°C with biotinylated goat anti-rabbit IgG secondary antibody (1:800, Vector Laboratories) in 5% normal goat serum in 0.1% Triton-X-100 in TBS ...
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bioRxiv - Neuroscience 2024Quote: ... and stored at 4 °C in VectaShield (Vector Laboratories).
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bioRxiv - Neuroscience 2020Quote: ... Muscles were kept in PBS at 4°C until mounted in Vectashied (Vector labs). Primary antibodies used in immunohistochemistry experiments are described in STAR methods ...
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bioRxiv - Molecular Biology 2020Quote: ... pre-cooled cells were incubated at 4°C with biotinylated tomato lectin (Vector Laboratories) at 2 μg/ml ...
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bioRxiv - Cell Biology 2021Quote: ... overnight at 4□C and secondary HRP-conjugated anti-rabbit IgG antibody (Vector Laboratories) for 1.5 h at RT ...
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bioRxiv - Microbiology 2020Quote: ... Detection was with biotinylated goat anti-rabbit IgG pAb (1h, 23°C, Vector Laboratories), Vectastain Elite ABC Peroxidase system ...
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bioRxiv - Immunology 2021Quote: ... boiled between 98°-102°C for 15 min with Antigen Unmasking Solution (Vector Laboratories), and permeabilized for 10 min at room temperature with PBS/2% Triton TX-100/1% sodium citrate ...
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bioRxiv - Physiology 2023Quote: ... slides are incubated in antigen-unmasking solution at 95°C (H-3300, Vector Laboratories Inc.) for antigen retrieval ...
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bioRxiv - Cell Biology 2021Quote: ... The embryos were mounted overnight at 4°C in Vectashield mounting medium (Vector Laboratories, Burlingame, USA), which contains DAPI ...
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bioRxiv - Cell Biology 2020Quote: ... overnight at 4°C and stained with fluorescein-conjugated streptavidin (Vector Laboratories, Peterborough, UK, 1:500). Cells were counterstained with ProLong® Gold Antifade Reagent with DAPI for nuclei staining (Cell Signalling Technology ...
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bioRxiv - Molecular Biology 2023Quote: ... for 1 hour at 37°C and ImmPACT DAB Peroxidase Substrate Kit (SK-4105, Vector Labs). Hematoxylin (H-3401 ...
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bioRxiv - Immunology 2023Quote: ... for 2 hours at 4°C and mounted with VECTASHIELD HardSet Antifade Mounting Medium (Vector Laboratories) followed by imaging with BZ-X800 microscope (Keyence) ...
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bioRxiv - Microbiology 2021Quote: ... pH 7.6 at 4°C overnight and goat anti-mouse IgG (#BA 9200, Vector Laboratories, Burlingame, CA) diluted 1:200 ...
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bioRxiv - Neuroscience 2021Quote: ... in PB for 2 days at 4°C (ABC reagent, VectaStain Elite ABC-HRP kit, Vector laboratories). After the 2 day incubation ...
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bioRxiv - Neuroscience 2023Quote: ... and blocked overnight at 4°C in a solution containing 5% Normal Goat Serum (NGS, Vector laboratories) and 0.5% Triton X-100 in PBS ...
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bioRxiv - Neuroscience 2023Quote: ... were added on tissues for 48 hours at 4°C after Mouse-On-Mouse Detection (Vector Laboratories). To label nuclei ...
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bioRxiv - Developmental Biology 2019Quote: ... Slides were then incubated at 55°C for 15 minutes and then mounted in VectaMount® (Vector Laboratories). Slides were stored at −20°C until imaged on Leica DFC295 color camera using brightfield microscopy through a 20x objective lens.
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bioRxiv - Cell Biology 2022Quote: ... tissue slides were incubated overnight at 60°C in Tris-based antigen unmasking solution (Vector Laboratories, Burlingame, CA) before permeabilization in 3% BSA ...
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bioRxiv - Neuroscience 2019Quote: ... Sections were incubated in primary antibodies for 48 h at 4°C and biotinylated secondary antibodies (Vector Labs) for 2 h at room temperature ...
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bioRxiv - Cancer Biology 2021Quote: ... and antigen retrieval was carried out at 100°C for 20 min with citrate buffer (pH6.0, Vector Laboratories). Sections were blocked with 0.1% bovine serum albumin (BSA ...
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bioRxiv - Neuroscience 2022Quote: ... Sections were incubated with primary antibodies for 48 h at 4□°C and biotinylated secondary antibodies (Vector Labs) for 2 h at room temperature ...
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bioRxiv - Cell Biology 2023Quote: ... Samples were then incubated overnight at 4°C with biotinylated isolectinB4 (Vector Labs, B-1205, diluted 1:50) and primary antibodies (Supplementary Table 2 ...
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bioRxiv - Genomics 2023Quote: ... three times in 2XSSC for 4 min at 42°C and mounted in Vectashield plus DAPI (Vector Laboratories).
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bioRxiv - Cell Biology 2023Quote: ... 3 μm paraffin sections were heated at 95°C in Antigen Unmasking Solution (Vector Laboratories, Burlingame, CA, USA) for 10-15 minutes ...
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bioRxiv - Immunology 2024Quote: ... overnight at 4 °C: The primary antibody was detected using biotinylated anti-rat secondary antibody (Vector Laboratories, BA40001.5) in conjunction with streptavidin horseradish peroxidase (ABC Elite ...
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bioRxiv - Molecular Biology 2021Quote: ... each for 5 min using a 42°C hot plate) before being mounted with VECTASHIELD with DAPI (Vector Labs) onto Superfrost Plus microscopy slides (VWR) ...
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bioRxiv - Cell Biology 2021Quote: ... and then incubated with sWGA agarose beads for 14 h at 4 °C with rotation (Vector laboratories, AL-1023S). The samples were centrifuged at 2500 × rpm for 5 min at 4 °C and washed five times with lysis buffer ...
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bioRxiv - Neuroscience 2020Quote: ... for 24 h at 4°C followed by goat biotinylated anti-rabbit antibody (1:250, Vector Laboratories, BA-1000) for 2h at RT ...
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bioRxiv - Neuroscience 2019Quote: ... Samples were left in 70% Glycerol/ 30% PBT overnight at 4°C prior to mounting with Vectashield (Vector Labs) and imaged with a Leica SP5 Microscope ...
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bioRxiv - Cell Biology 2021Quote: ... cryosections were incubated for 1 h at 37°C with rhodamine-conjugated PNA (1:2000; RL-1072, Vector Laboratories), and nuclear DNA was stained with DAPI (1:500 ...