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Corning RPMI 1640 with L Glutamine and 25 mM HEPES
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Amerigo Scientific Sponsored
Storage temperature of +2-8°CCat# 10-041-CM, 6 x 1L, Inquire Ask
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Corning
No products found because this supplier's products are not listed.bioRxiv - Cell Biology 2022Quote: ... which is RPMI 1640 supplemented with L-glutamine and 25 mM HEPES (Corning; Corning, NY) and containing 10% (v/v ... -
Thermo Fisher
No products found because this supplier's products are not listed.bioRxiv - Microbiology 2021Quote: ... RPMI 1640 medium supplemented with 25 mM HEPES and 2 mM L-glutamine (Gibco), 0.5% Albumax I (Gibco) ... -
Millipore Sigma
No products found because this supplier's products are not listed.bioRxiv - Cell Biology 2024Quote: ... at 5% haematocrit in RPMI 1640 complete medium containing L-glutamine and 25 mM HEPES (Sigma), supplemented with 0.2% sodium bicarbonate ... -
VWR
No products found because this supplier's products are not listed.bioRxiv - Immunology 2024Quote: ... and cells were transferred to medium (RPMI 1640 with L-glutamine and 25 mM HEPES; VWR International), supplemented with 5% human AB serum (Gemini Bio) ... -
Lonza
No products found because this supplier's products are not listed.bioRxiv - Biochemistry 2020Quote: ... Complete media consisted of RPMI 1640 without L-glutamine (Lonza), supplemented with 10% fetal bovine serum (FBS) ... -
Merck
No products found because this supplier's products are not listed.bioRxiv - Immunology 2024Quote: ... at a cell/bead ratio of 1:0.5 for 48-64h and expanded in complete R10 medium [RPMI-1640 medium containing 20 mM HEPES and 2.05 mM L-glutamine (#7388, Merck) supplemented with 10% iron-enriched bovine calf serum (BCS ... -
Genesee Scientific
No products found because this supplier's products are not listed.bioRxiv - Bioengineering 2022Quote: ... 2 mM L-glutamine (Genesee catalog #25-509) and 1x MEM non-essential amino acids (Genesee catalog #25-536) ... -
Agilent
No products found because this supplier's products are not listed.bioRxiv - Immunology 2024Quote: ... Stimulated BMDMs were washed with XF media (non-buffered RPMI-1640 containing 2 mM L-glutamine and 1 mM sodium pyruvate; Agilent Seahorse XF24) and incubated for 1 hour at 37°C ... -
Roche
No products found because this supplier's products are not listed.bioRxiv - Bioengineering 2024Quote: ... and placed into pre-weighed 1.5 mL polystyrene tubes containing 500 μL ice-cold digestion buffer (RPMI-1640 + 25 mM HEPES, pH 7.4, 1.3 WU/mL Liberase TL (Roche, Switzerland, Cat. No. 05401020001), 2 U/mL bovine DNase I (Roche ... -
Cambridge Isotope Labs
No products found because this supplier's products are not listed.bioRxiv - Cancer Biology 2024Quote: ... the cells were cultured in glucose and glutamine-free RPMI-1640 media supplemented with 1 mM labeled 13C5-L-Glutamine (Cambridge Isotope Laboratories Inc.), 10 mM unlabeled Glucose for 16 hours in triplicate ... -
Stemcell Technologies
No products found because this supplier's products are not listed.bioRxiv - Cell Biology 2024Quote: ... cells were treated with cardiomyocytes differentiation basic medium (2 mM L-glutamine RPMI-1640 plus 1x B27 minus insulin) containing 5 μM CHIR99021 (STEMCELL Technologies, Canada) for 48 hours ... -
GE Life Sciences
No products found because this supplier's products are not listed.bioRxiv - Biophysics 2022Quote: ... These cells were cultured in RPMI 1640 with 2.05mM L-glutamine (GE Healthcare Bio-Sciences Corp., MA, USA) supplemented by 5% v/v fetal bovine serum (FBS ... -
BioLegend
No products found because this supplier's products are not listed.bioRxiv - Microbiology 2024Quote: ... HEPES (10 mM) and L-glutamine (2 mM) supplemented with 25 ng/mL M-CSF (Biolegend, 574806) for 6 days. -
Peprotech
No products found because this supplier's products are not listed.bioRxiv - Immunology 2024Quote: ... filtered and cultured for 7 days in complete RPMI medium (RPMI-1640 medium containing L-glutamine plus 10% fetal calf serum) containing 20ng/mL recombinant GM-CSF (Peprotech). -
MP Biomedicals
No products found because this supplier's products are not listed.bioRxiv - Microbiology 2020Quote: ... Biofilm assays were performed in RPMI-1640 media (containing L-glutamine and lacking sodium biocarbonate, MP Biomedicals #0910601) supplemented with 34.5 g/L MOPS (Sigma ... -
R&D Systems
No products found because this supplier's products are not listed.Cited in Regulation of Decay Accelerating Factor primes human germinal center B cells for phagocytosisbioRxiv - Immunology 2020Quote: ... and RPMI-1640 supplemented as described and with additional 20 mM Hepes and 25 ng/ml M-CSF (R&D Systems). Medium was changed every third day and the cells were allowed to differentiate for 10 days ... -
Becton, Dickinson and Company
No products found because this supplier's products are not listed.bioRxiv - Cell Biology 2020Quote: ... 10 mM HEPES and 25% high protein Matrigel (product 354248; BD Biosciences). Subcutaneous injections of human melanoma cells were performed in the flank of NOD.CB17-Prkdcscid Il2rgtm1Wjl/SzJ (NSG ... -
Promega
No products found because this supplier's products are not listed.bioRxiv - Molecular Biology 2022Quote: ... The urea concentration was then diluted with 17 μl of 50 mM HEPES pH 8.5 and after adding 1 μl of 0.2 mg/ml trypsin (Promega) in 0.2 mM HCl the digestion continued overnight at 37°C with interval mixing at 2000 RPM for 30 s every 15 minutes ... -
Cayman Chemical
No products found because this supplier's products are not listed.bioRxiv - Cell Biology 2022Quote: ... 25 mM HEPES (Cayman Chemical), 100 U/mL penicillin and 100 µg/mL streptomycin (ScienCell) ... -
Alfa Aesar
No products found because this supplier's products are not listed.bioRxiv - Genomics 2023Quote: ... the following metabolites were added to match standard RPMI 1640 medium concentrations: 2mM L-glutamine (Alfa Aesar #J60573), 100.7µM L-methionine (Alfa Aesar #J61904) ... -
Calbiochem
No products found because this supplier's products are not listed.bioRxiv - Neuroscience 2021Quote: ... and 5 mM HEPES) supplemented with 0.423 mM L-cysteine (Calbiochem) and 5 U/mL papain (Worthington ... -
PerkinElmer
No products found because this supplier's products are not listed.bioRxiv - Immunology 2022Quote: ... L-[3,43H(N)]-glutamine (3H-glutamine; PerkinElmer, #NET551001MC) uptake was carried out using 1 million cells resuspended in 0.4 mL HBSS (Thermo ... -
anatrace
No products found because this supplier's products are not listed.bioRxiv - Biophysics 2024Quote: ... was equilibrated in a gravity flow column with 2 CVs of buffer B (150 mM NaCl, 25 mM HEPES pH 7.5, 0.06% (w/v) glyco-diosgenin (GDN) (Anatrace)) ... -
ibidi
No products found because this supplier's products are not listed.bioRxiv - Immunology 2022Quote: ... Cells were then washed twice with phenol red free RPMI 1640 medium and seeded in Poly-L-Lysine (PLL) coated μ-Slide 8 Well Glass Bottom Chambers (Ibidi). Cells were incubated at 37°C for 30 min to attach to the glass ... -
Takara Bio
No products found because this supplier's products are not listed.bioRxiv - Molecular Biology 2023Quote: ... 52 mM HEPES pH 7.4 (Takara), 35 mM potassium glutamate (Sigma) ... -
Bio-Rad
No products found because this supplier's products are not listed.bioRxiv - Biophysics 2020Quote: ... in GF150 buffer (25 mM HEPES pH 7.4, 150 mM KCl, 1mM MgCl2) on an NGC Chromatography system (Bio-Rad Laboratories, Hercules, CA, USA). Peak fractions were pooled ... -
Tocris
No products found because this supplier's products are not listed.bioRxiv - Developmental Biology 2024Quote: ... before changing medium on day 3 to RPMI 1640 with 1x B27 minus insulin and 5 mM IWP2 (Tocris 3533). On day 5 ... -
New England Biolabs
No products found because this supplier's products are not listed.Cited in CRISPR-based surveillance for COVID-19 using genomically-comprehensive machine learning designbioRxiv - Genomics 2020Quote: rNTP mix @ 25 mM each (NEB) -
Jena Bioscience
No products found because this supplier's products are not listed.bioRxiv - Molecular Biology 2024Quote: ... supplemented with 48 µg/ml L-cysteine and 4.08 mM L-alanyl-L-glutamine] supplemented with 50 µM homopropargylglycine (HPG) (Jena Bioscience) and 100 mg/ml anisomycin (Alomone Labs ... -
Beckman
No products found because this supplier's products are not listed.bioRxiv - Cancer Biology 2024Quote: ... Homogenates were adjusted to 42.5% sucrose by adding equal volumes of 85% sucrose in 2X HBS (25 mM HEPES, pH6.5, 150 mM NaCl) in an ultracentrifuge tube (344060, Beckman Coulter). 3.5 mL of 35% and 5% sucrose were overlayed to create a discontinuous sucrose gradient ... -
Gold Biotechnology
No products found because this supplier's products are not listed.bioRxiv - Immunology 2021Quote: ... and eluted with HEPES buffered saline (HBS, 50 mM HEPES, 150 mM NaCl, 0.5 mM TCEP (Gold Biotechnology, #TCEP10), pH 7.5 ... -
Cellvis
35 mm dish with 14mm micro-well without cover slip.Cat# D35-14, 100/case, $65.00 AskbioRxiv - Microbiology 2022Quote: ... albicans was diluted (1:100) in RPMI-1640 and transferred to glass bottom dishes (35 mm, In Vitro Scientific). The plates were incubated at 37 °C with supplementary 5% CO2 for 12 to 24 h to form mature biofilm ... -
Santa Cruz
No products found because this supplier's products are not listed.Cited in In vivo discovery of RNA proximal proteins in human cells via proximity-dependent biotinylationbioRxiv - Molecular Biology 2020Quote: ... were washed with 200 μL HBS (150 mM NaCl, 10 mM HEPES, pH7.5 by KOH) and incubated with 2 μg anti-HA (Santa Cruz, Cat.# sc-7392) or 2 μg rabbit IgG isotype (Thermo Fisher Scientific ... -
abcam
No products found because this supplier's products are not listed.bioRxiv - Immunology 2023Quote: ... or phenol red-free RPMI-1640 were serially diluted (2-fold) and mixed 1:1 with 0.2 mM nitrocefin (Abcam, Cambridge, UK). Samples were incubated 1–16 h at room temperature and absorbance at 490 nm was read on a SpectraMax M2 plate reader (Molecular Devices ... -
Leica
No products found because this supplier's products are not listed.bioRxiv - Neuroscience 2023Quote: ... 5 mM HEPES) using a vibratome (Leica VT1200S) and then incubated in an oxygenated recovery solution (130 mM NaCl ... -
Greiner
No products found because this supplier's products are not listed.Cited in Tau-Tubulin Kinase 2 restrains microtubule-depolymerizer KIF2A to support primary cilia growthbioRxiv - Cell Biology 2023Quote: ... 75/25 MM (Greiner Bio-One) at a high density (∼30.000 cells per well) ... -
Qiagen
No products found because this supplier's products are not listed.bioRxiv - Molecular Biology 2024Quote: ... 0.4 µl MgCl2 (25 mM; Qiagen), 0.8 µl Bovine Serum Albumine (BSA ... -
Jackson ImmunoResearch
No products found because this supplier's products are not listed.bioRxiv - Immunology 2021Quote: ... Cells were cultured in RPMI medium + Pen/Strep + L-glutamine + 10% FBS and stimulated with polyclonal goat anti-human IgM (Jackson Immunoresearch Affinipure; 0.6-75 ug/mL), or equimolar concentrations of the Fab’2 fragments (Jackson Immunoresearch Affinipure ... -
Electron Microscopy Sciences
No products found because this supplier's products are not listed.bioRxiv - Cell Biology 2021Quote: ... 4 mM NaHCO3, 1 mM NaH2PO4, 5 mM trehalose, 10 mM sucrose, 5 mM HEPES, pH 7.5, prepared by Electron Microscopy Sciences) and then bathed in whole organ ex vivo culture medium with 10 µg/ml isradipine (Selleck Chemical LLC ... -
BioSpec
No products found because this supplier's products are not listed.bioRxiv - Molecular Biology 2021Quote: ... and 400 μL of phenol:chloroform:isoamyl alcohol 25:24:1 and 300 μL of 0.5 mm zirconium ceramic beads (BioSpec Products, Cat # 11079105z) were added to the resuspension ... -
Phenomenex
No products found because this supplier's products are not listed.bioRxiv - Biochemistry 2020Quote: ... on a Agilent Technologies (GC-6890N, MS detector-5973) with a ZB-5 column (30 M x 25 mm x 25 mm, Phenomenex), with a temperature program of at 70 °C for 10 min followed by a gradient to 220°C at 5°C /min and held at 220°C for a further 15 min ... -
Nikon
No products found because this supplier's products are not listed.bioRxiv - Biochemistry 2024Quote: Fluorescence microscopy experiments were performed on a Nikon ECLIPSE Ti2 epifluorescence microscope equipped with a CFI60 Plan Fluor 40x Oil Immersion Objective Lens (N.A. 1.3, W.D. 0.2 mm, F.O.V. 25 mm; Nikon), a Spectra III UV ... -
MatTek
No products found because this supplier's products are not listed.bioRxiv - Cell Biology 2022Quote: ... 2.9 mM KCl, 5 mM HEPES, pH 7.2 (Westerfield, 2007) and mounted in 4-chamber glass bottom dishes (MatTek) for imaging different conditions in parallel (EGTA addition vs ... -
Harvard Apparatus
No products found because this supplier's products are not listed.bioRxiv - Biophysics 2023Quote: ... Pipettes were pulled from borosilicate glass (1.5 mm O.D. x 0.86 mm I.D. x 75 mm L; Harvard Apparatus) using a Sutter P-97 puller and heat-polished to final resistances between 1 and 5 MΩ using a MF-200 microforge (World Precision Instruments) ... -
World Precision Instruments
No products found because this supplier's products are not listed.Cited in Live-Cell Quantification Reveals Viscoelastic Regulation of Synapsin Condensates by α-SynucleinbioRxiv - Cell Biology 2024Quote: The micropipette was filled with the same buffer as the buffer for synapsin (25 mM Tris-HCl, 150 mM NaCl, 0.5 mM TCEP, pH = 7.4) using a MICROFIL needle (World Precision Instruments). The filled micropipette was then mounted onto a micromanipulator ... -
Eppendorf
No products found because this supplier's products are not listed.bioRxiv - Microbiology 2022Quote: ... 10 mM HEPES and seeded on confluent HFF monolayers in 8-well chamber slides (Eppendorf) at a density of 5×105 tachyzoites/well ... -
Olympus
No products found because this supplier's products are not listed.bioRxiv - Neuroscience 2024Quote: ... A 25× water-immersion objective lens (Olympus, 1.05 NA, 2 mm WD) was used to focus the excitation light into the brain and collect the emitted fluorescence ... -
Illumina
No products found because this supplier's products are not listed.bioRxiv - Plant Biology 2023Quote: ... The nuclei pellet was then immediately resuspended in 25 μ L of 2x tagmentation buffer containing 2 μ L of TDE1 (Illumina). The reaction was placed at 37C for 30 minutes with gentle mixing three times ... -
Sutter Instruments
No products found because this supplier's products are not listed.bioRxiv - Neuroscience 2023Quote: ... 0.87 mm) to a tip aperture of 20–25 μm using a Flaming–Brown puller (Sutter Instruments, Novato, CA, USA). The recording electrodes and a ground electrode were connected to two Warner DP-301 amplifiers ... -
Zymo Research
No products found because this supplier's products are not listed.bioRxiv - Cell Biology 2021Quote: ... Spheroplasting of harvested yeast cells was performed for 2 minutes in lysis buffer (1 M sorbitol 100 mM EDTA, 0.1% β-mercaptoethanol) containing 25 units of zymolyase (Zymo Research). RNA extraction was performed with an RNeasy kit (QIAGEN ...