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Citations for BioLegend :
1 - 50 of 372 citations for Corning RPMI 1640 with L Glutamine and 25 mM HEPES since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2024Quote: ... HEPES (10 mM) and L-glutamine (2 mM) supplemented with 25 ng/mL M-CSF (Biolegend, 574806) for 6 days.
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bioRxiv - Microbiology 2021Quote: ... malayi were individually incubated at 37 °C in RPMI 1640 with L-glutamine with 125 nM DRAQ5 (Biolegend) for 24-48 hrs ...
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bioRxiv - Immunology 2021Quote: ... plus 25 mM HEPES before incubating with TruStain FcX (BioLegend, 422302) for 5 minutes on ice ...
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bioRxiv - Microbiology 2020Quote: ... CD19+ and CD4− CD8− CD19− cells were resuspended in R-10 media (RPMI + 2 mM L-glutamine + 10% FBS + Penicillin/streptomycin) + 20 U/ml IL-2 (Biolegend, https://www.biolegend.com). Primary monocytes were resuspended in R-10 media ...
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bioRxiv - Bioengineering 2023Quote: ... were prepared in 1× Annexin V Binding Buffer (100 mM HEPES, 25 mM CaCl2, 1.4 M NaCl; BioLegend). To analyze cells ...
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bioRxiv - Immunology 2024Quote: Splenocytes and peripheral lymph node cells were cultured for 5 h at 37°C in complete medium (1640 RPMI + 10% FBS) plus 0.5 μM OVA257-264 and Monensin solution (1,000×; Biolegend) for cytokines detection ...
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bioRxiv - Immunology 2024Quote: ... which is RPMI-1640-based and supplemented with 5 μg/mL recombinant mouse IL-3 (#575506 or #575508, BioLegend) as described in previous studies (6 ...
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bioRxiv - Cell Biology 2020Quote: ... before plating the cells in petri dishes in RPMI 1640 medium containing 10 % heat-inactivated FCS and 50 ng/ml M-CSF (BioLegend) or 50 ng/ml GM-CSF (BioLegend) ...
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bioRxiv - Immunology 2021Quote: ... were produced by differentiating peripheral blood mononuclear cells (PBMC) in RPMI 1640 medium containing human M-CSF (10ng/mL, Biolegend) and 10% human platelets-free plasma for 5-7 days (42 ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... The suspended cells were then transferred to a fresh flask and activated in fresh RPMI-1640/10% FBS/PSG by 3:20,000 anti-CD3 (BioLegend 317303), 3:20,000 anti-CD28 (BioLegend 302913) ...
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bioRxiv - Cell Biology 2023Quote: ... at the indicated concentrations for 4 hours in 100 µL of RPMI 1640 medium containing 0.2% BSA with Brefeldin A Solution (BioLegend, 420601). After treatment ...
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bioRxiv - Cancer Biology 2023Quote: ... Jurkat transfectants were activated for 5 minutes at 37°C in serum-free RPMI-1640-sodium bicarbonate by crosslinking the anti-CD3 antibody (1μg/ml, clone UCHT1, BioLegend #300402) with secondary anti-mouse antibodies for immunoblot analysis of GSK-3 α/β and Akt phosphorylation.
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bioRxiv - Bioengineering 2024Quote: T-cells were cultured in RPMI-1640 media supplemented with 5 µg/mL brefeldin A (BioLegend, San Diego, CA, USA). After 24 hours of cultivation ...
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bioRxiv - Immunology 2022Quote: ... were cultured in RPMI-1640 medium supplemented with 10% fetal bovine serum (FBS) in the presence of 30 ng/ml GM-CSF (BioLegend) for 9-12 days ...
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bioRxiv - Cancer Biology 2023Quote: ... 2×106 bone marrow cells were cultured at 37°C in 10ml bmDC culture medium (RPMI 1640 medium with 10% FBS, penicillin/streptomycin and 2-Mercaptoethanol) with 40ng/mL GM-CSF (BioLegend). At day 3 ...
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bioRxiv - Bioengineering 2024Quote: Resting T-cells were cultured in RPMI-1640 media supplemented with 5 µg/mL brefeldin A (BioLegend, San Diego, CA, USA). After 24 hours of cultivation ...
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bioRxiv - Cancer Biology 2024Quote: ... Stimulated PBMCs were maintained in RPMI-1640 supplemented media with 100 ng/ml CD3 and 10 ng/ml recombinant human IL-2 (Biolegend, 589104).
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bioRxiv - Microbiology 2021Quote: ... were cultured in RPMI-1640 medium supplemented with 10% fetal bovine serum (FBS) in the presence of GM-CSF (20 ng/ml, BioLegend, Cat# 576304) for 9-12 days as described in (Dai et al. ...
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bioRxiv - Immunology 2021Quote: ... 2.1 mmol/L ultra-glutamine in the presence of Brefeldin A (5 μg/ml) and monensin (2 μM; BioLegend). After 5 hours of incubation ...
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bioRxiv - Immunology 2022Quote: ... and resulting cells were cultured in complete RPMI-1640 medium containing various concentrations of recombinant murine GM-CSF (rGM-CSF, BioLegend, San Diego, CA, USA), GPI-GM-CSF ...
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bioRxiv - Immunology 2022Quote: ... were generated from whole BM cells by cultivation in RPMI-1640-based media supplemented with 20 ng/mL mGM-CSF (BioLegend, San Diego, CA, USA) as previously described (Kanada et al ...
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bioRxiv - Bioengineering 2024Quote: ... the blocking solution was aspirated and chips were incubated flat overnight at 4° C with 25 µ L biotinylated αvβ3 antibody (1:50 dilution, 304412, BioLegend, San Diego, CA, USA) in the top channel in- and outlets and 15 µ L in the bottom channel in- and outlets ...
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bioRxiv - Genetics 2021Quote: ... CD4 (W3/25, Biolegend), CD8a (OX-8 ...
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bioRxiv - Biophysics 2024Quote: ... 15-25 mouse antibody (binds residues 15-25 on 2N4R Tau, BioLegend, 835201) as the primary antibody instead of mAb 423 ...
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bioRxiv - Immunology 2020Quote: ... The lower chamber was prepared with either 150μl RPMI complete or 150μl RPMI complete containing SDF1α (100ng/ml, Biolegend). The transwell plate was placed in a 37 °C incubator and cells were allowed to migrate for 2 h ...
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bioRxiv - Pharmacology and Toxicology 2024Quote: ... CD4 (clone W3/25) (Biolegend), CD8 (clone OX-8 ...
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bioRxiv - Pharmacology and Toxicology 2024Quote: ... CD4 (clone W3/25) (Biolegend), CD8 (clone OX-8 ...
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bioRxiv - Cell Biology 2024Quote: ... 25 ng/ml thrombopoietin (BioLegend) and 0.1 mM 1-thioglycerol (Sigma ...
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bioRxiv - Cancer Biology 2024Quote: ... + 25 µl PI solution (BioLegend) + 5 µl RNase A (VWR International ...
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bioRxiv - Cancer Biology 2022Quote: ... 0.01% 55 mM 2-mercaptoethanol) supplemented with rmGM-CSF (50 ng/mL) and rmFlt3-L (200ng/mL) (Biolegend, San Diego CA). The culture medium was changed on day 9 of culture ...
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bioRxiv - Cancer Biology 2024Quote: ... REF #3421) containing RPMI medium (5% FBS) or RPMI medium (5% FBS) supplemented with rmCXCL13 (1000 ng/ml) (BioLegend #583902) for 5 minutes ...
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bioRxiv - Cancer Biology 2024Quote: ... was filled with RPMI (5% FBS) or RPMI (5% FBS) medium containing rhCXCL12 (200 ng/ml) or rhCXCL13 (1000 ng/ml) (Biolegend). Cell suspension of 5*105 cells or 1.5*105 cells for normal human B cells ...
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bioRxiv - Immunology 2020Quote: ... IL-8 (Biolegend; 25 ng/mL).
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bioRxiv - Immunology 2022Quote: ... anti-CD44 (25-0441-82, BioLegend), anti-CD69 (104506 ...
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bioRxiv - Immunology 2024Quote: ... IL-25 (Biolegend, San Diego, CA), soluble IL-6R ...
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bioRxiv - Cancer Biology 2023Quote: ... APC-CCR5 (1:25; BioLegend, #556903); PerCP-CD4 (1:100 ...
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bioRxiv - Physiology 2023Quote: ... AnnexinV-FITC (1:25, BioLegend, 640905); Propidium Iodide (1:50 ...
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bioRxiv - Cell Biology 2024Quote: ... 25 ng/ml IL-3 (BioLegend), 25 ng/ml IL-6 (BioLegend) ...
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bioRxiv - Cell Biology 2024Quote: ... 25 ng/ml IL-6 (BioLegend), 25 ng/ml thrombopoietin (BioLegend ...
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bioRxiv - Cancer Biology 2022Quote: ... and 25 mg/ml propidium iodide (Biolegend). After 30 min incubation at room temperature in the dark ...
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bioRxiv - Neuroscience 2021Quote: ... human CD45-FITC (BioLegend; dilution 1:25) and human CD31-V450 BD Biosciences ...
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bioRxiv - Immunology 2020Quote: ... rIL-12 (25 ng/ml; Biolegend, #575402), rIL-18 (25 ng/ml ...
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bioRxiv - Bioengineering 2023Quote: ... CD11c-AF700 (1:25, clone 3.9, Biolegend), CD45RA-APC (1:50 ...
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bioRxiv - Bioengineering 2023Quote: ... CD14-BV510 (1:25, clone M5E2, Biolegend), CD66b-BV421 (1:50 ...
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bioRxiv - Immunology 2023Quote: ... 25 U/mL IL-2 (575404, BioLegend) and 50 μM 2-Mercaptoethanol ...
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bioRxiv - Microbiology 2023Quote: ... and 25 ng/mL M-CSF (Biolegend). Cells were incubated at 37°C with 5% CO2 for 5 days with media changes on days 3 and 5 ...
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bioRxiv - Immunology 2024Quote: ... anti-Tbet (4B10, Biolegend 25-5825-80), anti-Gata3 (TWAJ ...
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bioRxiv - Microbiology 2024Quote: ... and 25 ng/mL M-CSF (Biolegend). Cells were incubated at 37°C with 5% CO2 for 5 days with media changes on days 3 and 5 ...
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bioRxiv - Microbiology 2023Quote: ... 5 µg of IL-25 (#587306; BioLegend), or sterile PBS as a vehicle control ...
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bioRxiv - Physiology 2023Quote: ... anti-VCAM1-PE (1:25, BioLegend, 305805); anti-E-Selectin-PE (1:25 ...