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Citations for Beckman :
1 - 50 of 507 citations for Corning RPMI 1640 with L Glutamine and 25 mM HEPES since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cancer Biology 2024Quote: ... Homogenates were adjusted to 42.5% sucrose by adding equal volumes of 85% sucrose in 2X HBS (25 mM HEPES, pH6.5, 150 mM NaCl) in an ultracentrifuge tube (344060, Beckman Coulter). 3.5 mL of 35% and 5% sucrose were overlayed to create a discontinuous sucrose gradient ...
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bioRxiv - Molecular Biology 2020Quote: ... and 4 mM magnesium acetate) were prepared in 25 × 89 mm polyallomer tubes (Beckman Coulter) using a Gradient Master (BioComp) ...
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bioRxiv - Microbiology 2022Quote: ... and 25% (wt/wt in 12.5ml) sucrose gradient in 25 mM citrate buffer in 38 ml Ultraclear tubes (Beckman-Coulter, 344058). The gradient was centrifuged at 100,000 × g for 18 h at 4°C (Beckman-Coulter XPN-80 Ultracentrifuge ...
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bioRxiv - Biochemistry 2023Quote: ... transferred to 25 x 89 mm Ultra-Clear thin-wall tubes (Beckman # 344058) (typically 38 mL per tube ...
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bioRxiv - Neuroscience 2024Quote: ... for 2 h using 25 ξ 89 mm polycarbonate tubes (Beckman Coulter, 355618). Pellets were resuspended in 500 μL of sterile phosphate-buffered saline (PBS ...
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bioRxiv - Evolutionary Biology 2020Quote: ... in 67 mM HEPES-KOH pH 7.2) after 2 h of ultracentrifugation (4 °C, 5000 RPM, Beckman SW41 ...
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bioRxiv - Biophysics 2024Quote: ... and 100 mM HEPES pH 7.0) prepared in house using a Biomek liquid handler instrument (Beckman Coulter). CDK2-cyclin A complex at 10 mg mL-1 was mixed at a ratio of 1:1 with precipitant solution ...
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bioRxiv - Neuroscience 2020Quote: ... 5 mM HEPES (pH 7.4)] and centrifuged for 18 hrs at 100,000 x g (SW 32.1 Ti; Beckman Coulter) with no brake ...
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bioRxiv - Cell Biology 2021Quote: ... About 9 ml interface that came from 3×SW28 tubes (25×89 mm, Beckman Coulter) was loaded in a SW41 tube (14×89 mm ...
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bioRxiv - Microbiology 2021Quote: ... resuspended in 150 μl 0.1 M HEPES/NAOH pH 7.0 and purified for 48 h (250,000 × g, 4 °C; Beckman Optima LE-80K, rotor SW60-Ti) using a CsCl gradient (0.45 g/ml ...
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bioRxiv - Cancer Biology 2023Quote: ... Samples were sedimented onto poly-L-lysine coated coverslips at 12,000 rpm (22,579 x g) in an Avanti J-25 centrifuge (Beckman Coulter) for 1 hr and post-fixed with methanol for 5 min at −20 °C ...
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bioRxiv - Cell Biology 2023Quote: ... and the supernatants were combined and transferred to 25 × 83-mm centrifuge tubes (cat. 344367, Beckman), followed by centrifuge at 95,000g on a SW 32 Ti rotor (Beckman ...
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bioRxiv - Plant Biology 2022Quote: ... diluted four times with 5 mM HEPES-KOH pH = 7.5 and centrifuged at 21,5000 rpm for 20 min (Beckman Coulter JA 25.50).
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bioRxiv - Biochemistry 2022Quote: ... 000 g at 4°C for 30 min in a Beckman Coulter Optima L- 100 XP ultracentrifuge using 14 mm × 89 mm Ultra-Clear Centrifuge Tubes (Beckman Instruments Inc.). Ultra-pure mitochondria were harvested at the interphase between 19% and 40% and washed three times with 1xM buffer ...
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bioRxiv - Biochemistry 2020Quote: ... 150 μL of the lysate was layered onto a 600 μL sucrose cushion (25% sucrose, 20mM tris pH 8.0, 10mM MgCl2, 100 mM NH4Cl) in a TLA100.2 rotor (Beckman) and centrifuged for 1 h at 100,000 rpm and 4°C ...
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bioRxiv - Immunology 2022Quote: ... The homogenized tissue was transferred into open-top thick-walled polycarbonate ultracentrifuge tubes (25×89 mm, Beckman Coulter) on ice ...
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bioRxiv - Immunology 2021Quote: ... were loaded onto 5-25% (w/v) linear sucrose gradients and centrifuged in a Beckman Optima L-90K Ultracentrifuge (Beckman SW40 rotor) at 40,000 rpm for 3 h 15°C ...
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bioRxiv - Cell Biology 2020Quote: ... and 25% Histodenz™ in SHE buffer was layered in 14 Å∼ 89 mm Ultra-Clear centrifuge tubes (Beckman) and the tubes were sat at 4°C for 3–4 h to allow the Histodenz™ to diffuse ...
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Syntaxin-2 inhibits excessive phagocytic uptake but promotes phagolysosomal clearance in macrophagesbioRxiv - Cell Biology 2024Quote: ... followed by sequential topping with 2 mL each of 35, 25, and 10% sucrose (all prepared in 3 mM imidazole, pH 7.4) in SW40 tubes (344060, Beckman). The samples were then centrifuged at 100,000 g for 1 hr at 4°C using a SW40Ti rotor (Beckman) ...
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bioRxiv - Molecular Biology 2021Quote: ... Sucrose solutions were made in Buffer G and 25 x 89 mm 38.5 mL open-up polyallomer tubes were used (Beckman Coutler). Ultra-centrifugation was performed (160,000 x g ...
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bioRxiv - Microbiology 2023Quote: ... A volume of 30 mL of supernatant was dispensed in two 38.5 mL ultracentrifuge tubes (Ultra-Clear, 25×89 mm, 344058, Beckman Coulter) before adding 5 mL of a 20% sucrose solution in NTE buffer (100 mM NaCl ...
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bioRxiv - Microbiology 2024Quote: ... Supernatants were layered over a 20% sucrose cushion (in PBS) and concentrated by ultracentrifugation by spinning in 25 x 89 mm ultraclear tubes (Beckman) at 28 K rpm for 2 h at 4°C in an SW32 swing-bucket rotor (Beckman) ...
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bioRxiv - Genomics 2021Quote: ... Cells were washed with 25 mm supplemented HBSS before depleting the sample of unnucleated cells using IOTest 3 Lysing solution (Beckman Coulter) following provider instructions ...
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bioRxiv - Microbiology 2022Quote: ... The recovered supernatant of both induced and uninduced cultures was transferred in 6 polyallomer (36 ml each) centrifuge tubes (25×89 mm, Beckman Coulter) to which 1 ml of Protease Inhibitor Cocktail (Sigma Aldrich ...
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bioRxiv - Microbiology 2024Quote: ... The solution was then given a final concentration of 25 mM MgCl2 and ultracentrifuged at 200,000 x g for two hours (4°C) (Beckman Coulter Type 45 Ti). The pellet was then resuspended in 100 μl of distilled water.
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bioRxiv - Physiology 2023Quote: ... via centrifugation in a SW41 rotor at 42,000 g at 4 °C for 1 hour in a Beckman Coulter Optima L-100 XP ultracentrifuge using 14 × 89 mm Ultra-Clear Centrifuge Tubes (Beckman Instruments Inc.). Mitochondria were harvested at the interphase between 19 and 40% Percoll ...
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bioRxiv - Biophysics 2020Quote: ... transferred into 25×89mm polyallomer ultracentrifuge tubes (Beckman) and ultracentrifuged in an Optima XL-100K ultracentrifuge (Beckman Coulter ...
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bioRxiv - Microbiology 2024Quote: ... 4 °C (Beckman Coulter, JA 25-50 rotor) for 10 min ...
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bioRxiv - Microbiology 2024Quote: ... 4°C (Beckman Coulter, JA 25-50 rotor) for 10 min and the cell lysates were incubated with 1 mL of prewashed Ni-NTA Agarose beads (QIAGEN ...
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bioRxiv - Plant Biology 2022Quote: ... The cDNA was eluted from the beads with 25 µL RNase-free water and purified again with 25 µL (1.0X volume) of Ampure XP beads (Beckman Coulter Life Sciences) then incubated at RT for 15 min then washed and dried as described above ...
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bioRxiv - Microbiology 2022Quote: ... Avanti-J-25 centrifuge (Beckman Coulter, Palo Alto, USA). The viral pellet was resuspended in EBM-2 without supplements ...
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bioRxiv - Cancer Biology 2023Quote: ... 25mM HEPES (pH 7.0) and 1% FBS and sorted with the Astrios EQ (Beckman Coulter) into RPMI1640 (Corning ...
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bioRxiv - Immunology 2024Quote: ... washed in complete RPMI and counted on a Vi-CELL (Beckman Coulter) to determine total viable cell number ...
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bioRxiv - Genomics 2024Quote: ... (25) DNA fragments were purified using Ampure XP beads (Beckman Coulter - prediluted 1 in 8 in bead binding buffer – 1 M NaCl ...
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bioRxiv - Biochemistry 2024Quote: ... After centrifugation (Optima L-90K, Beckman Coulter), the three layers were separated through carefully pipetting 80µL from the top of the sample per fraction and mixing with SDS loading buffer (Laemmli buffer ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... and the lymphocytes fixed (25 μl of Fixative Solution, Beckman Coulter). The samples were mixed and incubated for 10 minutes in the darkness ...
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bioRxiv - Microbiology 2023Quote: ... Cell lysate was centrifuged at 17,000 rpm (JA-25, Beckman Coulter) for 1 hr and the supernatant was filtered using 0.45 µm filter before subjecting it to Ni-NTA resin (Thermo ...
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bioRxiv - Neuroscience 2021Quote: ... The resulting homogenate was mixed with 6 mL sucrose (2 mol/L) and 2.5 mL CaCl2 (0.1 mmol/L) in an ultra-clear centrifuge tube (#344059, Beckman Coulter, USA). Then ...
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bioRxiv - Biochemistry 2021Quote: ... and membranes collected by ultracentrifugation (Beckman L-90K) at 152,947 g for 40 min at 4 °C ...
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bioRxiv - Biophysics 2020Quote: ... and a L-100 XP ultracentrifuge (Beckman Coulter) equipped with an SW 32 Ti swinging bucket rotor operating at 28.000 rpm ...
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bioRxiv - Microbiology 2023Quote: ... The lysate was then centrifuged at 17,000 rpm (JA-25, Beckman Coulter) for 1 hr to remove cell debris ...
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bioRxiv - Microbiology 2024Quote: ... Bacteria were pelleted at 4000xg for 20 min (Beckman Avanti J-25) and resuspended in 30 mL of purification buffer (20 mM HEPES ...
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bioRxiv - Microbiology 2021Quote: ... the wafer clump was collected using a pipette tip and resuspended in 3 mL of 2x Buffer C and mixed with 3 mL of 1x Buffer C (20 mM MES-Tris, 10 mM MgCl2, 50 mM KCl, pH 6.9) and pelleted in the ultracentrifuge (Beckman 50Ti). The resulting pellet was then resuspended in 200 µL of 1x Buffer C with 10% glycerol ...
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bioRxiv - Cell Biology 2020Quote: ... Samples were centrifuged for 20 h at 190,000 x g in a SW-40 Rotor (Beckman Optima L-70 or Beckman Optima L-80 XP, Beckman Coulter, Brea, CA) at 4 °C ...
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bioRxiv - Cell Biology 2022Quote: ... in a Optima L-80 XP ultracentrifuge (Beckman Coulter) for 16 h at 100.000 g and collected using an auto Densi-Flow peristaltic pump fraction collector with a meniscus-tracking probe (Labconco ...
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bioRxiv - Cell Biology 2020Quote: ... 4°C (SW32Ti rotor, Optima L-80XP ultracentrifuge, Beckman) with the maximum acceleration and minimum deceleration ...
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bioRxiv - Genetics 2020Quote: ... using an Optima L-100 XP Ultracentrifuge (Beckman Coulter) and the SW41Ti rotor ...
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bioRxiv - Microbiology 2021Quote: ... ultracentrifuged (Beckman Coulter Optima L-100k; SW32 Ti rotor) for 2 h at 25,000 rpm (80,000 x g ...
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bioRxiv - Genetics 2021Quote: ... using an Optima L-100 XP Ultracentrifuge (Beckman Coulter) and the SW41Ti rotor ...
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bioRxiv - Pathology 2021Quote: ... ultracentrifuged (Beckman Coulter Optima L-100k; SW32 Ti rotor) for 2 h at 25,000 rpm (80,000 X g ...