Labshake search
Citations for Qiagen :
1 - 50 of 754 citations for Corning RPMI 1640 with L Glutamine and 25 mM HEPES since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Molecular Biology 2024Quote: ... 0.4 µl MgCl2 (25 mM; Qiagen), 0.8 µl Bovine Serum Albumine (BSA ...
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bioRxiv - Genetics 2021Quote: ... 1.5 μl of 25 mM MgCl2 (Qiagen), 0.3 μl each of 10 μM forward and reverse primers (Table 1) ...
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bioRxiv - Genetics 2023Quote: ... 1 ul of 25 mM MgCl2 (Qiagen) and 1 ul of the primer mix (40 uM of the Forward primer ...
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bioRxiv - Biophysics 2023Quote: ... The supernatant was diluted 3 times with buffer A (20 mM HEPES, pH 7.4, 200 mM NaCl, 1 mM Asp) and incubated with Ni-NTA resin (Qiagen) for 1 hour at 4 °C ...
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bioRxiv - Immunology 2023Quote: ... The periplasmic fraction was filtered with a 0.22 um filter and incubated with 4 mL 50% Ni-NTA resin equilibrated in wash buffer (20 mM HEPES, pH 7.5, 150 mM NaCl, 40 mM imidazole) (Qiagen) per liter of initial bacterial culture ...
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bioRxiv - Cancer Biology 2023Quote: ... we adapted the same conditions used in Dip-C40 and lysed each nucleus in 150 nL of lysis buffer containing 20 mM Tris pH8/20 mM NaCl/25 mM DTT/0.15% Triton X-100/1 mM EDTA/5 µg/mL Qiagen Protease (Qiagen, cat. no. 19157). After dispensing ...
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bioRxiv - Developmental Biology 2021Quote: ... Remote biopsies were taken using an air-powered rifle affixed with a 1 mm wide by 20-25 mm deep biopsy dart and preserved in RNAlater (Qiagen). Blood samples were collected opportunistically during veterinarian visits ...
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bioRxiv - Developmental Biology 2021Quote: ... Skin samples were taken by remote biopsy dart (1 mm wide by 20-25 mm deep plug) and preserved in RNAlater (Qiagen). Blood samples were taken opportunistically during veterinarian visits and preserved in EDTA tubes ...
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bioRxiv - Genetics 2023Quote: ... leaf tissues were collected and homogenized in 400 μL of TPS buffer (100 mM Tris-HCl, 10 mM EDTA, 1 M KCl, pH8.0) using TissueLyser II (Qiagen), followed by incubation for 20 min at 75°C ...
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bioRxiv - Physiology 2024Quote: ... weighed and homogenized 1:180 (weight:volume, mg:µL) in homogenization buffer (50 mM Triethanolamine and l mM EDTA) with 2 tungsten beads (Qiagen, Cat. #69997) using a Tissue Lyser (Qiagen Cat ...
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bioRxiv - Microbiology 2023Quote: ... 25 mM NaCl in UltraPure distilled water) with 0.4 mg/ml Proteinase K (QIAGEN N.V., Venlo, NL). Homogenized samples were incubated at 37 °C for 1.5 h followed by 98 °C for 2 min ...
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bioRxiv - Physiology 2021Quote: ... and homogenized with metal beads (2.4 mm diameter, Askubal) for 2 min at 25 Hz in a TissueLyzerII (Qiagen). Beetles were weighed and frozen in liquid nitrogen ...
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bioRxiv - Microbiology 2022Quote: ... homogenized in 500 μl PBS by bead beating (3 mm steel ball, 25 Hz for 2.5 min in a TissueLyser (Qiagen)) ...
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bioRxiv - Evolutionary Biology 2020Quote: ... The entire flies were mechanically crushed for 30 s at 25 Hz using a 5-mm stainless steel bead in a TissueLyser (Qiagen). Three hundred μL of ACL solution and 20 μL of 16 g.L-1 proteinase K were then added to the samples ...
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bioRxiv - Microbiology 2021Quote: ... Emulsions were formed immediately after by shaking tubes at 25 Hz for 30 seconds on a Retch Mixer Mill MM 400 with adapters 11990 and 11993 (Mobio/Qiagen). Next ...
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bioRxiv - Microbiology 2021Quote: ... Reactions were emulsified at 25 Hz for 30 seconds on a Retch Mixer Mill MM 400 with adapters 11990 and 11993 (Mobio/Qiagen). Emulsion tubes were photographed before and after thermocycling and assayed by eye for coalescence ...
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bioRxiv - Immunology 2021Quote: ... homogenized in 1 ml PBS by bead beating (3 mm steel ball, 25 Hz for 1 min in a TissueLyser (Qiagen)) ...
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bioRxiv - Microbiology 2023Quote: ... total RNA was extracted from triplicate samples at mid-log phase growth when succinate concentrations reached approximately 25 mM using the RNeasy Mini Kit (Qiagen) according to the manufacturer’s instructions ...
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bioRxiv - Neuroscience 2023Quote: ... for 2 x 2 minute intervals at 25 Hz with the addition of one 7 mm stainless steel bead (Qiagen #69990). 200 uL of tissue lysate was used for KingFisher Flex (Thermo Fisher Scientific ...
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bioRxiv - Pharmacology and Toxicology 2020Quote: ... 0.1 g of tissue per 1 mL of PBS was homogenized with stainless steel beads of 5 mm (25–30 Hz for 5 min) by a Tissuelyser (Qiagen, Venlo, Netherlands). The homogenates were centrifuged at 10,000 x g for 10 min and the supernatant was collected and stored at −80 °C until CD quantification ...
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bioRxiv - Molecular Biology 2021Quote: ... 25 μL Multiplex Mastermix (Qiagen Multiplex PCR Plus Kit ...
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bioRxiv - Immunology 2024Quote: ... Plasmids were expanded using Stlb3 competent cells and maxi-prep from Qiagen (QIAGEN Plasmid Maxi Kit (25)) ...
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bioRxiv - Molecular Biology 2022Quote: ... Biotin-labelled RNA was eluted with 100 μl elution buffer (100 mM DTT in nuclease-free water) and cleaned up with the RNeasy MinElute Cleanup kit (QIAGEN), adjusting the amount of ethanol to capture RNA < 200 nucleotides in length ...
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bioRxiv - Microbiology 2022Quote: For RNAseq the pellets were resuspended in 150 µL 10 mM Tris-HCl pH 8 and mixed with 700 µL of ice cold RLT+BME (RLT buffer (Qiagen) supplemented with 1% β-mercaptoethanol ...
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bioRxiv - Microbiology 2024Quote: ... 1x NEAA and 20mM HEPES) were homogenized using the TissueLyser II (Qiagen). Blood samples were initially diluted (1:10 ...
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bioRxiv - Cell Biology 2021Quote: ... 25 nM of S1PR1 #1 (Qiagen, #SI00376201) 5’-ATGATCGATCATCTATAGCAA-3’ and 25nM S1PR1 #2 (Qiagen ...
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bioRxiv - Biophysics 2024Quote: ... resuspended in 25 μL P1 buffer (Qiagen) and lysed with P2 buffer ...
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bioRxiv - Neuroscience 2021Quote: ... clusterProfiler (v3.12.14) (25) and Ingenuity Pathway Analysis (Qiagen).
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bioRxiv - Molecular Biology 2021Quote: ... and eluted in 25 µl of EB (Qiagen). The resulting sample was quantified using a Quant-iT PicoGreen dsDNA assay (Thermo Fisher Scientific) ...
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bioRxiv - Genetics 2024Quote: ... 25 ng of XPC and GAPDH (Qiagen, France) cDNAs were then used to launch the qPCR reaction using gene-specific primers along with the Platinum SYBR green qPCR SuperMix-UDG (#Cat.11733038 ...
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bioRxiv - Microbiology 2021Quote: ... leaf-disc punches were collected from plants and ground in 250 µL of 10 mM MgCl2 using the TissueLyser II (QIAGEN; 2 cycles of 30 seconds at 25 Hz) and 3-mm zirconium oxide beads (Glen Mills Inc.) ...
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Enhancing gene transfer to renal tubules and podocytes by context-dependent selection of AAV capsidsbioRxiv - Cell Biology 2023Quote: ... and 25 pmol primers in a 25 μL reaction volume and subjected to qPCR using Rotor-Gene Q (Qiagen, Venlo, Netherlands). We amplified the tdTomato gene sequence for vector genome quantification using the following primers ...
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bioRxiv - Immunology 2024Quote: ... or sorted into either complete RPMI or RLT lysis buffer (Qiagen) or Trizol using a BD Aria III.
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bioRxiv - Molecular Biology 2024Quote: ... 2.8□l of forward and reverse primer at 5□M concentration and 10□l QuantiNova (Qiagen), made up to a final volume of 20□l with nuclease-free water ...
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bioRxiv - Bioengineering 2020Quote: ... The samples were ground by bead beating for 25 sec at 25 Hz using a Tissue Lyser II machine (Qiagen, Hilden, Germany). For CFU-based bacterial enumeration ...
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bioRxiv - Biochemistry 2021Quote: ... 25% PEG 8000 from the PEG suite screen (Qiagen) after 48 hours at 18°C ...
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bioRxiv - Cancer Biology 2024Quote: ... RNase A (25 µg/mL final concentration; Qiagen, 19101) was added ...
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bioRxiv - Genomics 2023Quote: ... Button valves were opened and biotinylated anti-pentaHis antibody (Qiagen, 1:4 dilution in HEPES) was flowed for 30 minutes ...
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bioRxiv - Genomics 2020Quote: ... DNA was eluted in 25 μL of AVE buffer (Qiagen).
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bioRxiv - Genomics 2021Quote: ... and DNA eluted in 25 µL nuclease-free H2O (Qiagen). When required ...
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bioRxiv - Neuroscience 2024Quote: ... at 25 Hz for 1.5-3min (Qiagen/Retsch Bead Beater). Following homogenization samples were centrifuged at 16,000 x g for 10 mins at 4 °C ...
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bioRxiv - Neuroscience 2024Quote: ... at 25 Hz for 1.5-3min (Qiagen/Retsch Bead Beater). Following homogenization samples were centrifuged at 16,000 x g for 10 mins at 4 °C ...
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bioRxiv - Genomics 2023Quote: ... using a TissueLyzer II (QIAGEN, 2min, 25 Hz, 2 times). The samples were incubated at room temperature for 5min ...
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bioRxiv - Microbiology 2022Quote: ... 25% (w/v) PEG 1500 (QIAGEN PACT screen, condition 42).
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bioRxiv - Systems Biology 2021Quote: ... and 200 L of Buffer AL (QIAGEN) were added to each sample before mixing with a pipette ...
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bioRxiv - Microbiology 2023Quote: ... DNA was eluted in 25 mL of nuclease-free water (Qiagen). DNA concentration was measured using Qubit dsDNA HS Assay kit (Thermo Fisher Scientific) ...
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bioRxiv - Microbiology 2023Quote: ... DNA was eluted in 25 mL of nuclease-free water (Qiagen). DNA concentration was measured using Qubit dsDNA HS Assay kit (Thermo Fisher Scientific) ...
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bioRxiv - Biochemistry 2023Quote: Ni-NTA agarose resin 25 ml (Qiagen, Germany; cat. no. 30210)
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bioRxiv - Molecular Biology 2024Quote: 25 mg tissue sections were bead-lysed in RLT buffer (Qiagen) supplemented with 1% (w/w ...
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bioRxiv - Molecular Biology 2024Quote: ... or 25 nM of miR-125b-5p mimic (Qiagen, Hilden, Germany) or a combination of 25 nM of miR-125b-5p mimic and 10 nM of miR-214 mimic (Qiagen ...