-
No products found
because this supplier's products are not listed.
Ge Song, et al.,
bioRxiv - Immunology 2020
Quote:
... Proteins without His tags were purified with GNL columns (Vector Labs). The bound proteins were washed with PBS and then eluted with 50 mL of 1M Methyl α-D-mannopyranoside (Sigma M6882-500G ...
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No products found
because this supplier's products are not listed.
Jessie J.-Y. Chang, et al.,
bioRxiv - Immunology 2023
Quote:
... propagation of the virus was carried out in Vero (African green monkey kidney epithelial – ATCC: CCL-81) cells cultured in MEM (MP Biomedicals), supplemented with 1 µg/mL TPCK-Trypsin (Trypsin-Worthington) ...
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No products found
because this supplier's products are not listed.
Fujun Hou, et al.,
bioRxiv - Immunology 2022
Quote:
A35R protein (OkayBio, C1620) and M1R protein (OkayBio, C1624) were diluted to 5 μg/ ml by ELISA coating buffer (Elabscience, E-ELIR-003) and added into 96-well plates for overnight coating at 4 degree ...
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No products found
because this supplier's products are not listed.
Benjamin Craske, et al.,
bioRxiv - Biochemistry 2022
Quote:
... anti–His tag antibodies (Raybiotech, 168-10481) at a 1:10 dilution in BRB80 were first introduced to the chamber ...
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No products found
because this supplier's products are not listed.
Takeshi Katsuda, et al.,
bioRxiv - Cell Biology 2023
Quote:
... and 1 μl E-Coli spike-in DNA (0.5 ng/μl) (EpiCypher) to each tube ...
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No products found
because this supplier's products are not listed.
Luyao Huang, et al.,
bioRxiv - Plant Biology 2023
Quote:
... the purified recombinant mCherry or Tip6ΔSP-mCherry proteins were separately mixed with purified His-ZmTPL2N-His proteins and incubated with pre-washed magnetic mCherry-Trap beads (Chromotek, Cat. No. rtmak) in wash buffer (0.2 M NaH2PO4/0.2 M Na2HPO4 ...
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No products found
because this supplier's products are not listed.
James B. Winans, et al.,
bioRxiv - Microbiology 2022
Quote:
... was stained with Anti-6X His Epitope Tag (Rabbit) antibody conjugated to Dylight 405 (Rockland Immunochemicals, cat. # 600-446-382) added to the media at a concentration of .1μg/mL for the entirety of the experiment ...
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No products found
because this supplier's products are not listed.
Candice R. Gurbatri, et al.,
bioRxiv - Bioengineering 2023
Quote:
... and stained with either H&E or specific immunohistochemistry markers (HA-Tag C29F4 #3724 from Cell Signaling Technology; GranzymeB Leica Biosystems PA0291 ...
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No products found
because this supplier's products are not listed.
Kelly Mitchell, et al.,
bioRxiv - Cancer Biology 2022
Quote:
... and virus was concentrated with PEG-it Virus Precipitation solution (System Biosciences). SORE6-GFP virus was added to CSCs plated on Geltrex ...
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No products found
because this supplier's products are not listed.
Donia Hidaoui, et al.,
bioRxiv - Cancer Biology 2024
Quote:
CUT&Tag assays were performed using CUT&Tag-IT assay kit (Active Motif) on 50,000 CD34+ cells according to manufacturer’s instructions ...
-
No products found
because this supplier's products are not listed.
Feng Li, et al.,
bioRxiv - Genetics 2019
Quote:
A Hi-C library was constructed with the ProxiMeta Hi-C kit from Phase Genomics v 1.0 containing the enzyme Sau3A ...
-
No products found
because this supplier's products are not listed.
Anna Lipońska, et al.,
bioRxiv - Microbiology 2024
Quote:
... For proteins with his-tag we used anti-6xHis-tag primary antibody (Covalab, ref. HIS.HS/ EH158) in dilution 1:2 000 detected with anti-mouse IRdye 800CW (LI-COR ...
-
No products found
because this supplier's products are not listed.
Andrea M. Chambers, et al.,
bioRxiv - Immunology 2021
Quote:
... Avi-His-Tag biotin labeled antibody (BPS Bioscience) for 30 minutes at 37⁰C ...
-
No products found
because this supplier's products are not listed.
Huan Wang, et al.,
bioRxiv - Biochemistry 2021
Quote:
... anti-His tag (Solarbio Life Sciences, China, K200060M), anti-toll-like receptor-4 (TLR-4 ...
-
No products found
because this supplier's products are not listed.
Yongping Ma, et al.,
bioRxiv - Immunology 2021
Quote:
... 100 µl of rabbit anti-His-tag antibody (1:1000, BioVision, USA, cat#3998) was incubated at 37℃ for 2 h and washed as described above ...
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No products found
because this supplier's products are not listed.
Patrick J. Flynn, et al.,
bioRxiv - Cell Biology 2021
Quote:
... Centromere Protein E Inhibitor (GSK923295; Cayman Chemicals), Actin Depolymerizer (Cytocholasin-D ...
-
No products found
because this supplier's products are not listed.
Nikol Dibus, et al.,
bioRxiv - Cell Biology 2023
Quote:
... Purified proteins were then eluted by desthiobiotin using Buffer E (IBA Lifesciences) and subsequently prepared for immunoblotting as described above ...
-
No products found
because this supplier's products are not listed.
Vilde Leipart, et al.,
bioRxiv - Biochemistry 2022
Quote:
... We made three sets of blank samples: buffer blank and two sets with SUMO-1 (human, His-tag, Enzo Life Sciences). One collection of SUMO-1 samples were incubated with 25uM ZnCl2 at 4 °C for 1 hour ...
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No products found
because this supplier's products are not listed.
Yu-Feng Chien, et al.,
bioRxiv - Bioengineering 2020
Quote:
... a TAG lens (TAG lens 2.5β, TAG Optics) was placed directly on top of an objective (UPLFLN 10XP, Olympus) to enable high-speed axial scan of the focus ...
-
No products found
because this supplier's products are not listed.
Alexis S. Gorin, et al.,
bioRxiv - Neuroscience 2023
Quote:
... Virus was injected iontophoretically (Nanoject II, Drummond Scientific) via a glass micropipette (tip diameter ∼15-20 μm ...
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WB,IF,IP,IHC,FC,ELISA
Cat# A5712, SKU# A5712-20ul,
20ul, $47.00
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Xuxiao He, et al.,
bioRxiv - Cancer Biology 2020
Quote:
... and Nickel Magnetic Beads for His tag protein purification (Bimake, B23602) according to the manufacturer’s protocol ...
-
No products found
because this supplier's products are not listed.
Anil Verma, et al.,
bioRxiv - Immunology 2023
Quote:
... were labeled with biotinylated anti-His tag monoclonal antibody (ThermoScientific) and used to capture His-tagged clade C gp120 Du151 protein (Immune Technologies). The gp120-expressing beads were then incubated with triplicate 5-fold dilutions of heat-inactivated serum samples in V-bottom plates for 1h at 37°C ...
-
No products found
because this supplier's products are not listed.
Joshua D. Powell, et al.,
bioRxiv - Microbiology 2024
Quote:
... NJ) and were seronegative to IAV antibodies by a commercial ELISA kit (Swine Influenza Virus Ab Test, IDEXX) prior to the start of the study ...
-
No products found
because this supplier's products are not listed.
Motokazu Uchigashima, et al.,
bioRxiv - Neuroscience 2020
Quote:
... Tag-blue fluorescent protein (Tag-BFP, Evrogen), Nlgn3Δ ...
-
No products found
because this supplier's products are not listed.
Sabal Chaulagain, et al.,
bioRxiv - Microbiology 2020
Quote:
Swine PBMCs were obtained from swine whole blood through density gradient centrifugation using Sepmate15 (Stem cell technologies) and lymphocyte separation media (Corning) ...
-
No products found
because this supplier's products are not listed.
FWN Chow, et al.,
bioRxiv - Molecular Biology 2019
Quote:
... Quantification was carried by western blot analysis in comparison to known concentrations of recombinant full length exWAGO containing an N-terminal 3XFlag-His tag using Image Studio Lite (LI-COR) software ...
-
No products found
because this supplier's products are not listed.
Vijayendra Dasari, et al.,
bioRxiv - Immunology 2022
Quote:
... protein was concentrated and filtered through Mustang E membrane (PALL Corporation, NY, USA) to further remove endotoxin contaminants ...
-
No products found
because this supplier's products are not listed.
Hongzhuang Peng, et al.,
bioRxiv - Cancer Biology 2020
Quote:
... The recombinant human BAP1-FL-WT protein was purchased from Boston Biochem (E-345-050). The Duet-His-ULD/AB protein complex was purified under native purification conditions using Cobalt beads (Talon) ...
-
No products found
because this supplier's products are not listed.
Liubov Cherkashchenko, et al.,
bioRxiv - Molecular Biology 2023
Quote:
Vero cells (African green monkey kidney cells, ATCC CCL-81) were grown in Dulbecco’s modified Eagle’s medium (DMEM, Lonza) supplemented with 10% foetal bovine serum (FBS ...
-
No products found
because this supplier's products are not listed.
Jamie C. Moffa, et al.,
bioRxiv - Neuroscience 2023
Quote:
... Virus was removed from the upper chamber of the concentrator and stored in low protein binding tubes (Eppendorf #022431064) at -80°C.
-
No products found
because this supplier's products are not listed.
Pieter Nibbering, et al.,
bioRxiv - Plant Biology 2020
Quote:
... 18µl α-Fucosidase (E-FUCTM) and 20µl α-Arabinofuranosidase (E-ABFAN) from Megazyme in a 50 mM sodium acetate buffer pH5 at 37 °C for 4 hours (again ...
-
No products found
because this supplier's products are not listed.
Lok R. Joshi, et al.,
bioRxiv - Immunology 2021
Quote:
... ultra-centrifugation of virus culuture supernatant and the virus pellet in 30% sucrose cushion gradient were performed using Optima-L 100K ultracentrifuge (Beckman Coulter) at 18,000 RPM for 1.5 hours ...
-
No products found
because this supplier's products are not listed.
Seungyoon B. Yu, et al.,
bioRxiv - Neuroscience 2023
Quote:
... coverslips were transferred to Hibernate E (BrainBits) medium containing 5nM TMRM as described previously 60 ...
-
No products found
because this supplier's products are not listed.
S.N. Freda, et al.,
bioRxiv - Neuroscience 2022
Quote:
... Virus was injected using a microsyringe pump (World Precision Instruments); >5 minutes elapsed before the pipette was moved from the ventral injection site to the more dorsal injection site ...
-
No products found
because this supplier's products are not listed.
Michael P. Doyle, et al.,
bioRxiv - Immunology 2022
Quote:
... his-tagged YFV E protein (Meridian Life Science) was associated to the tips at 5 μg/mL for 60 sec ...
-
No products found
because this supplier's products are not listed.
Chunhua Shi, et al.,
bioRxiv - Pathology 2020
Quote:
... Lipopolysaccharide (LPS; from E. coli O111:B4, Cedarlane;) the principal component of Gram-negative bacteria that activates the innate immune system via TLR4 ...
-
No products found
because this supplier's products are not listed.
Michael P. Doyle, et al.,
bioRxiv - Immunology 2022
Quote:
... The soluble recombinant E protein was recovered by 6-HIS affinity chromatography on Ni-NTA agarose (G-Biosciences) and purified by size exclusion chromatography on a Superdex S200 Increase column (Cytiva).
-
No products found
because this supplier's products are not listed.
Satoshi Imanishi, et al.,
bioRxiv - Pharmacology and Toxicology 2022
Quote:
... and the corresponding His tag (R06-32BH) were obtained from SignalChem Lifesciences (Richmond ...
-
No products found
because this supplier's products are not listed.
Daisuke Ogasawara, et al.,
bioRxiv - Biochemistry 2024
Quote:
... N-term strep-His tag was first inserted into phCMV3 vector (Genlantis, cat # P003300) using Q5® Site-Directed Mutagenesis Kit (New England BioLabs ...
-
No products found
because this supplier's products are not listed.
Jürgen G. Okun, et al.,
bioRxiv - Physiology 2020
Quote:
... or protein-enriched (80%E protein, 10% carbohydrate, 10% fat; D16080303, Research Diets, USA) diets for a period of 3 weeks with various metabolic phenotyping tests ...
-
No products found
Fang Wu, et al.,
bioRxiv - Biochemistry 2023
Quote:
The following primary antibodies were used for Western blotting: anti-His Tag (Applied Biological Materials, #G020; 1:4000), anti-HA Tag (Applied Biological Materials ...
-
No products found
because this supplier's products are not listed.
Kaihuai Li, et al.,
bioRxiv - Microbiology 2020
Quote:
... Protein transfer from the Mn2+ phos-tag acrylamide (APExBIO, Houston, USA) gel to the PVDF membrane (Millipore ...
-
No products found
because this supplier's products are not listed.
Oh Sung Kwon, et al.,
bioRxiv - Cell Biology 2020
Quote:
... To repress protein synthesis 100 μg/ml of cycloheximide (TOKU-E) and 300 μM of puromycin (InVivoGen ...
-
No products found
because this supplier's products are not listed.
Mariska S. Simpson, et al.,
bioRxiv - Cell Biology 2023
Quote:
... His-tagged proteins were affinity purified by binding to cobalt resin (GoldBio) equilibrated in 1X PBS ...
-
No products found
because this supplier's products are not listed.
Ronald L. Shanderson, et al.,
bioRxiv - Cancer Biology 2024
Quote:
Recombinant proteins fused with either Flag or Flag-HA-His tags were transfected at 20 µg per 15 cm plate using Polyethyleneimine 25000 (23966-100, Polysciences) solution at 2.5 µg per 1 µg of DNA ...
-
No products found
because this supplier's products are not listed.
Samad A. Ibitokou, et al.,
bioRxiv - Immunology 2022
Quote:
... or (15 ng/mouse) of recombinant-mouse IFN-γ (rIFN-γ expressed in E. coli; PeproTech, Rocky Hill, NJ), i.p ...
-
No products found
because this supplier's products are not listed.
Lydia M. Castelli, et al.,
bioRxiv - Systems Biology 2021
Quote:
... compound E 0.1 µM (Cpd E; Tocris), BDNF 10ng/mL (ThermoFisher Scientific) ...
-
No products found
because this supplier's products are not listed.
Nathaniel Kastan, et al.,
bioRxiv - Developmental Biology 2020
Quote:
... Ad-RFP virus (Vector Biolabs) at the same titer were used as a control.
-
No products found
because this supplier's products are not listed.
Violaine Tribollet, et al.,
bioRxiv - Cell Biology 2020
Quote:
E-plates 16 PET (ACEA Biosciences) were coated with 1.5 µg/cm2 collagen I (Corning ...
-
No products found
because this supplier's products are not listed.
Prashant P. Damke, et al.,
bioRxiv - Microbiology 2022
Quote:
... using a microplate reader (BioTek Synergy HI) to record the absorbance at 650 nm ...