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Citations for Active Motif :
1 - 50 of 77 citations for African Swine Fever Virus P72 Protein His tag E. coli since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cancer Biology 2024Quote: CUT&Tag assays were performed using CUT&Tag-IT assay kit (Active Motif) on 50,000 CD34+ cells according to manufacturer’s instructions ...
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bioRxiv - Cell Biology 2023Quote: Cut&Tag was performed using a Complete CUT&Tag-IT Assay Kit (Active Motif) according to manufacturer’s instructions ...
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bioRxiv - Cell Biology 2023Quote: CUT&Tag libraries were prepared using the CUT&Tag-IT Assay Kit (Active Motif, 53160) following to the manufacturer’s protocol ...
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bioRxiv - Cell Biology 2021Quote: ... coli (31243, Active Motif), GST-VRK1 produced in insect Sf9 cells (ab125555 ...
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bioRxiv - Genomics 2022Quote: ... The basal kit for CUT&Tag used was a CUT&Tag-IT Assay Kit (Active Motif). After washing the zona-free blastocysts with phosphate-buffered saline containing 0.01% (w/v ...
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bioRxiv - Genomics 2023Quote: ... The basal kit for CUT&Tag was a CUT&Tag-IT Assay Kit (Active Motif, Carlsbad, CA). Dig-Wash buffer and Dig-300 buffer in the kit were supplemented with 0.01% (w/v ...
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bioRxiv - Cancer Biology 2023Quote: ... coli (Cat# 31493, Active Motif) was mixed with 5 μl of ProteoJuice in 100 μl of OPI-MEM and incubated at room temperature for 20 min ...
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bioRxiv - Cell Biology 2022Quote: CUT&Tag-IT assay kit (Active Motif) was used on 3,000 HSC according to manufacturer’s instructions ...
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bioRxiv - Cell Biology 2024Quote: ... His-tone H2A.Z (1:200, Active Motif #39013), Nkx2.5 (1:250 ...
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bioRxiv - Neuroscience 2023Quote: ... 100,000-125,000 nuclei were used for each CUT&Tag reaction using the CUT&Tag IT Assay Kit (Active Motif, #53610) per manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2021Quote: ... Immunoprecipitations for H2A.Z and HA-tag were performed similarly on benzonase-treated yeast cell extract using 36µl Protein-A sepharose beads coupled with 6µl of appropriate antibody (Anti-H2A.Z, Active Motif 39647; Anti-HA tag, Abcam ab9110). Control immunoprecipitations (mock ...
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bioRxiv - Neuroscience 2022Quote: ... centrifuged 500 g for 5 min and washed a final time with 1 mL 1X CUT&Tag wash buffer (from CUT&Tag IT Assay Kit, Active Motif, #53610) or 1 mL CUT&RUN 1X wash buffer (1 mL HEPES pH 7.5 ...
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bioRxiv - Neuroscience 2022Quote: ... and the CUT&Tag-IT Assay Kit (Active Motif) following the manufacture’s manual ...
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bioRxiv - Cell Biology 2022Quote: ... with anti-His and anti-T120 phosphoantibody (Active motif, 39391).
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bioRxiv - Cell Biology 2023Quote: ... with anti-His and anti-T120 phosphoantibody (Active motif, 39391).
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bioRxiv - Genetics 2024Quote: CUT&Tag analysis of NaBu treated CCD841 cells and mouse intestinal samples were performed using CUT&Tag-IT™ assay kits (Active Motif # 53160, 53170). Approximately 30 mg of tissue was homogenized per reaction by first chopping frozen tissue into smaller pieces in a petri dish with a razor blade on dry ice ...
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bioRxiv - Neuroscience 2023Quote: ... nuclei pellet was washed once with 1 mL Nuclei Wash Buffer and then once with 1 mL of 1X CUT&Tag Wash Buffer (from Active Motif CUT&Tag IT Assay kit). Remove CUT&Tag Wash Buffer ...
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bioRxiv - Molecular Biology 2020Quote: ... wells were coated with 30 nM of His-tagged DNMT1 (Active Motif) or HSA overnight at 4°C ...
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bioRxiv - Cancer Biology 2023Quote: ... KURAMOCHI and OVCAR4 Hi-C were conducted and processed by Active Motif using the Arima-Hi-C kit and library prep performed with technical duplicates (Arima Genomics) ...
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bioRxiv - Biophysics 2022Quote: ... and P1 virus was generated from cells transfected with FuGENE transfection reagent (Active Motif) according to the manufacturer’s instructions ...
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bioRxiv - Genomics 2023Quote: ... 0.5 million cells were processed using the CUT&Tag-IT kit (Active Motif) as per manufacturer’s instructions and the resulting libraries were paired-end sequenced on a NextSeq500 platform (Illumina ...
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bioRxiv - Molecular Biology 2020Quote: ... Recombinant Human DNMT1 with an N-terminal GST tag was purchased from Active Motif and Pierce Glutathione Magnetic Beads (Thermo Scientific ...
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bioRxiv - Genomics 2022Quote: ... we used CUT&Tag-IT™ assay kit (Cat. No. 53160) from Active Motif® ...
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bioRxiv - Neuroscience 2022Quote: CUT&Tag was performed from 450k nuclei using the Cut&TagIT Kit following manufacturer’s protocol (Active Motif).
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bioRxiv - Neuroscience 2024Quote: Cells from proliferating iMOPs and iMOP-derived neurons were harvested for and processed using the CUT&Tag-IT Assay Kit (Active Motif) described by the manufacturer ...
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bioRxiv - Biochemistry 2023Quote: ... recombinant AGO2 protein (Active Motif) was purchased and serially diluted in Protein LoBind tubes (Eppendorf ...
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bioRxiv - Molecular Biology 2022Quote: Cleavage under targets and tagmentation (CUT&Tag [35]) was performed for three biological replicates per experimental condition using the CUT&Tag-IT Assay Kit (Anti-Rabbit) (Active Motif, 53160) according to the manufacturer’s instructions with some modifications ...
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bioRxiv - Genetics 2022Quote: ... the nuclei suspension was diluted in a 1X volume of 1X Wash Buffer from a CUT&Tag-IT Assay Kit (Active Motif 53160). To estimate the yield of nuclei ...
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bioRxiv - Microbiology 2022Quote: ... parasites were centrifuged 2000xg for 10min and the parasite pellet used directly with the CUT&Tag-IT Assay Kit (Active Motif 53160). Our experimental sample used TgBDP13xHA parasites and 1ul of a 1:50 dilution of the rabbit anti-HA primary antibody (Cell Signaling 3724T) ...
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bioRxiv - Microbiology 2022Quote: ... Parasite pellets were then processed as per the manufacturer’s protocol (CUT&Tag-IT™ Assay Kit, Anti-Rabbit, Active motif, Cat no. 53160). Libraries were sequenced by Azenta at PE 2×150 bp.
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bioRxiv - Microbiology 2021Quote: Nuclear proteins were isolated from challenged A549 cells using a nuclear protein extraction kit (Active Motif) according to manufacturer’s specifications and protein concentration was determined using a bicinchoninic acid (BCA ...
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bioRxiv - Neuroscience 2023Quote: ... myelin basic protein (Active Motif, 31314) was employed as a substrate for recombinant CDKL5 ...
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bioRxiv - Molecular Biology 2023Quote: CUT&Tag was performed as described previously (Kaya-Okur et al., 2020; Kaya-Okur et al., 2019) using the CUT&Tag-IT Assay Kit from Active Motif (Catalog No. 53160) and following manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2024Quote: ... H3K27ac histone modification data was generated from liver sinusoidal endothelial (LSEC) and liver-resident immune cryopreserved cells using the Cut&Tag-IT Assay kit (Active Motif, H3K27ac antibody cat#39133). Library products were assessed by Agilent Bioanalyzer HS DNA assays (Agilent Technologies ...
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bioRxiv - Molecular Biology 2020Quote: ... 0.8μM of purified DNMT1 protein (Active Motif) was incubated with 30000 cpm of 32P-labelled aptamers and single or double-stranded ...
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bioRxiv - Cell Biology 2023Quote: The recombinant proteins p53 (#81091, Active Motif), PITPα (#ab101666 ...
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bioRxiv - Biochemistry 2023Quote: ... and Protein G beads (Active motif, #53014) were added for an additional 2 hours ...
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bioRxiv - Cell Biology 2020Quote: The following commercially available proteins were used in this study: recombinant SETD2 (a.a. 1418-1714) protein (Active Motif, 31358), rabbit skeletal muscle actin (Cytoskeleton ...
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bioRxiv - Cancer Biology 2020Quote: ... 30μl of Protein G Magnetic Beads (Active Motif) were washed twice with 500μl CoIP buffer and incubated with Antibody-containing lysat for 1 hour at 4°C with gentle rotation ...
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bioRxiv - Plant Biology 2019Quote: ... Added antibody-conjugated protein G beads (Active Motif 61085 for Pol II Ser5p ...
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bioRxiv - Cell Biology 2023Quote: ... recombinant CDKL5 and myelin basic protein (Active Motif, 31314) as a substrate were incubated in a kinase buffer (Cell Signaling ...
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bioRxiv - Cell Biology 2021Quote: ... At the same time protein G agarose beads (Active Motif) were washed and blocked with 5% BSA in ChIP buffer and left to rotate O/N in 4°C ...
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bioRxiv - Genomics 2019Quote: ... or 100-750ng YY1 full-length recombinant protein (31332, Active Motif) in binding buffer containing 10 mM Tris (pH 7.5) ...
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bioRxiv - Biochemistry 2022Quote: Recombinant AGO (AGO1, AGO2, AGO3) protein was purchased from Active Motif (AGO1 Catalog No ...
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bioRxiv - Neuroscience 2023Quote: ... Mitochondrial proteins were extracted using Nuclear Extract Kit (Active Motif, Germany) according to manufacturer instructions ...
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bioRxiv - Microbiology 2020Quote: ... including Protein A magnetic beads as per manufacturer’s instructions (Active Motif, USA). The input and immunoprecipitated DNA were quantified by real-time PCR using a Stratagene MX3500P PCR System ...
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bioRxiv - Immunology 2021Quote: ... Chromatin was pre-cleared using 30 μl protein G agarose beads (Active Motif) pre-blocked with BSA per manufacturer’s instructions ...
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bioRxiv - Microbiology 2023Quote: ... Nuclear proteins were isolated according to the manufacturer instruction (Active Motif nuclear extraction). Nrf2 TransAM ELISA-kit (Active Motif ...
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bioRxiv - Molecular Biology 2021Quote: ... ubiquitinated or unmodified Clr4 proteins were mixed with Recombinant Mononucleosomes (H3.1, Active Motif 31467) or Recombinant Mononucleosomes H3K9me3 (H3.2 ...
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bioRxiv - Cancer Biology 2021Quote: ... Nuclear and cytoplasmic protein lysates were prepared using the Nuclear Extract Kit (Active Motif) or Nuclear Complex Co-IP Kit (Active Motif) ...