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Citations for Gold Biotechnology :
1 - 50 of 78 citations for African Swine Fever Virus P72 Protein His tag E. coli since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2023Quote: ... His-tagged proteins were affinity purified by binding to cobalt resin (GoldBio) equilibrated in 1X PBS ...
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bioRxiv - Synthetic Biology 2023Quote: ... coli were 50 µg/ml kanamycin (GoldBio) or 100 µg/ml carbenicillin (GoldBio) ...
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bioRxiv - Microbiology 2022Quote: ... coli: 200 μM isopropyl beta-D-1-thiogalactopyranoside (IPTG, GoldBio), 0.1% L-arabinose (Sigma) ...
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bioRxiv - Bioengineering 2024Quote: ... at 1 mg/mL and elastase at 6 U/mL (E-240, Goldbio) for 1 h ...
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bioRxiv - Biophysics 2020Quote: ... coli in the presence of 100 μg/mL ampicillin (GoldBio, Olivette, MO). The cultures were incubated at 37 °C ...
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bioRxiv - Biochemistry 2023Quote: ... coli and isolated using nickel agarose beads (Goldbio, Cat# H-350-5). 5 μM of HaBlap was mixed with 50 μM nitrocefin in 5 mM phosphate buffer pH 7 ...
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bioRxiv - Molecular Biology 2023Quote: ... coli (ZymoResearch T3001) cells and plated on selection media (Ampicillin, Goldbio 69-52-3). Sanger sequencing confirmed plasmids that contained the correct protease insert ...
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bioRxiv - Molecular Biology 2023Quote: ... coli (ZymoResearch T3001) cells and plated on selection media (Ampicillin, Goldbio 69-52-3). A green-white screen46 was used to inoculate assembled colonies in selection liquid media ...
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bioRxiv - Microbiology 2020Quote: ... alongside a protein molecular weight marker (BLUEstain protein ladder, Gold Biotechnology). Gels were run at 100 V for 15 min and then 150 V for 40 min in NuPAGE MES SDS running buffer and the proteins were subsequently transferred onto PVDF or nitrocellulose membranes using iBlot transfer stacks (Thermo Fisher Scientific) ...
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bioRxiv - Microbiology 2021Quote: ... coli TOP10 and plated on LB agar plates supplemented with 100 μg/mL hygromycin B (GoldBio, USA). An individual colony was picked and transferred to 5 mL of LB medium supplemented with 100 μg/mL hygromycin B and incubated overnight at 37 °C ...
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bioRxiv - Immunology 2021Quote: ... RBD proteins were purified using protein A agarose resin (Goldbio CAT# P-400), followed by on-column cleavage with HRV3C protease (made in-house ...
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bioRxiv - Microbiology 2022Quote: ... The virus-serum mix was subsequently added to Vero cells for 48 h before 50 nmoles of D-Luciferin (GoldBio, St Lous, MO, USA) was added to measure luminescence ...
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bioRxiv - Microbiology 2020Quote: ... Secreted hACE2-Fc and mACE2-Fc proteins were purified by protein A chromatography (Goldbio).
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bioRxiv - Biochemistry 2022Quote: ... 20 mM KCl and home-made protease inhibitor cocktail (500 μM AEBSF, 1 μM E-64, 1 μM Leupeptin, 150 nM Aprotinin) (Gold Biotechnology). Total cellular membranes were harvested by ultracentrifugation ...
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bioRxiv - Microbiology 2023Quote: ... coli strains were inoculated from an agar colony plate into 300 mL BHI media with 100 ug/mL carbenicillin (GoldBio, 00901C103) and 100 uM isopropyl β-d-1-thiogalactopyranoside (IPTG ...
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bioRxiv - Synthetic Biology 2021Quote: ... coli S17-1 λ pir was routinely grown in Luria Bertani (LB) broth (LabExpress) supplemented with 100 μg/mL carbenicillin (Gold Biotechnology) for plasmid selection.
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bioRxiv - Molecular Biology 2021Quote: Purification of the mouse lectin-mIgG2a fusion proteins was performed using Protein A agarose resin (Gold Biotechnology, P-400-5). The protein A beads were pelleted at 150g for 5 min and washed once with 1x binding buffer (0.02 M Sodium Phosphate ...
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bioRxiv - Cancer Biology 2024Quote: ... Protein expression was induced with IPTG (GoldBio, I2481C) at 16°C for 18 hours ...
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bioRxiv - Immunology 2021Quote: ... followed by purification with protein A agarose resin (GoldBio). Expi293F cells were cultured in Expi293 Expression Medium (Gibco ...
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bioRxiv - Biophysics 2020Quote: ... Protein expression was induced by adding 0.2 % arabinose (Goldbio) for 3 hr at 37 °C ...
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bioRxiv - Immunology 2021Quote: ... and mAbs were purified with protein A agarose (GoldBio).
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bioRxiv - Immunology 2020Quote: ... and incubated overnight with Protein A Agarose Resin (GoldBio). The resin was collected in a chromatography column ...
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bioRxiv - Microbiology 2023Quote: ... and incubated overnight with Protein A Agarose Resin (GoldBio). The resin was collected in a chromatography column ...
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bioRxiv - Microbiology 2023Quote: ... and incubated overnight with Protein A Agarose Resin (GoldBio) at 4°C ...
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bioRxiv - Cell Biology 2020Quote: ... or Protein A Agarose Resin (Gold Biotechnology, P-400-5). Beads and antibody were incubated and rotated at room temperature for 10 minutes ...
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bioRxiv - Microbiology 2020Quote: ... Bulk IgGs were captured by protein A agarose resin (GoldBio) at 4°C rotating overnight ...
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bioRxiv - Molecular Biology 2021Quote: ... Heterologous protein expression was induced with 0.5 mM IPTG (GoldBio), and cultures were grown overnight at 19°C with gentle agitation.
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bioRxiv - Molecular Biology 2021Quote: ... Protein expression was induced by adding 0.5 mM IPTG (GoldBio) and grown overnight at 16°C ...
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bioRxiv - Molecular Biology 2021Quote: ... Protein expression was induced by adding 1 mM IPTG (GoldBio) and grown at 37°C for 5 h with agitation.
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bioRxiv - Molecular Biology 2021Quote: ... Protein expression was induced by adding 0.5 mM IPTG (GoldBio) followed by growth overnight at 16°C ...
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bioRxiv - Immunology 2022Quote: ... and antibody was purified using protein A agarose chromatography (Goldbio). Sequences were obtained from PCR reaction products and annotated using the ImMunoGeneTics (IMGT)/V- QUEST database (http://www.imgt.org/IMGT_vquest/)51,52 ...
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bioRxiv - Immunology 2023Quote: WP and HIP were mixed with Protein A-agarose (GoldBio), Protein G-agarose (Invitrogen) ...
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bioRxiv - Microbiology 2021Quote: ... Amersham) and protein expression induced with 1 mM final IPTG (Goldbio) for 4 hr at 37 °C ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... The supernatant was filtered and passed over Protein A resin (GoldBio), washed with 10 mM sodium phosphate pH 7.5 ...
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bioRxiv - Biochemistry 2023Quote: ... the protein was purified using glutathione agarose resin (Gold Biotechnology Inc.). The protein was subjected to TEV cleavage to remove the GST tag and then further purified using a Mono Q 5/50 GL anion exchange column (GE Healthcare Life Sciences) ...
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bioRxiv - Biochemistry 2022Quote: ... The digested protein was passed over a 2-mL Ni-HTC (GoldBio) gravity column to separate the tag-free NnlA ...
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bioRxiv - Immunology 2021Quote: ... The cleaved product was incubated with 1mL of Protein A resin (GoldBio) per 10mg of initial IgG and incubated at room temp for 1 hr to bind any uncleaved IgG and digested Fc ...
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bioRxiv - Microbiology 2020Quote: ... clarified cell lysates were purified by protein G affinity chromatography (Gold Biotechnology, Inc.). The b12 Fab ...
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bioRxiv - Immunology 2020Quote: ... The RBD was purified using protein A agarose resin (GoldBio, Cat# P-400) and cleaving the Fc domain using HRV3C protease (made in house ...
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bioRxiv - Biochemistry 2021Quote: ... The supernatant containing GST-Rev1 protein was incubated with glutathione agarose resin (Goldbio) for 2 hours ...
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bioRxiv - Molecular Biology 2021Quote: ... expression of recombinant protein was induced with 0.5 mM isopropyl β-D-thiogalactoside (GoldBio). For purification of PABPC1 and ePAB ...
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bioRxiv - Biochemistry 2020Quote: ... Induction of protein expression was induced by addition of 1 mM IPTG (Gold Biotech) and cells were grown overnight at 18°C ...
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bioRxiv - Biochemistry 2022Quote: ... protein expression was induced with 1 mM IPTG (isopropyl β-D-1-thiogalactopyranoside; GoldBio) followed by expression for 17 hr at 25°C ...
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bioRxiv - Biochemistry 2022Quote: ... followed by protein induction with 0.5 mM isopropyl ß-D-1-thiogalactopyranoside (Gold Biotechnology) at 18°C for 16 hrs ...
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bioRxiv - Molecular Biology 2023Quote: ... Proteins were eluted by adding lysis buffer containing 35 mM reduced glutathione (Gold Biotechnology). The elute fractions were run on a 4-12% NuPAGE Bis-Tris protein gel (Invitrogen ...
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bioRxiv - Biophysics 2019Quote: ... protein expression was then induced with Isopropyl-β-D-1-thiogalactopyranoside 0.1 mM (GoldBio, Canada) for 12 h at room temperature ...
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bioRxiv - Microbiology 2021Quote: ... Lysates were clarified using centrifugation and proteins were purified via nickel agarose beads (Gold Biotechnology), eluted with 300mM imidazole ...
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bioRxiv - Microbiology 2020Quote: ... along with 2.5 μl of a pre-stained protein ladder (#P008; GoldBio, St. Louis, MO), and separated on an SDS-polyacrylamide gel ...
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bioRxiv - Immunology 2022Quote: ... 4.5 mg E1D1 antibody was incubated with 1 mL Protein A resin (GoldBio, P-400) with rotation at room temperature for 30 minutes and then washed thoroughly with PBS ...
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bioRxiv - Biochemistry 2021Quote: ... Lipin 1 and lipin 1 ΔM-Lip proteins were purified using Ni-NTA resin (GoldBio). Eluted protein was applied to Streptactin-XT resin equilibrated with equilibration buffer (100 mM Tris ...