-
No products found
because this supplier's products are not listed.
Morgan E. Mouchka, et al.,
bioRxiv - Evolutionary Biology 2019
Quote:
... Colonies were screened on LB plates with 50 μg/mL ampicillin and 9.5 mg/mL X-gal (5-bromo-4-chloro-3-indolyl-b-D-galactopyranoside; APExBIO) and Sanger sequencing of the J was used to confirm cloning and successful transformation of the ancestral λ sequence.
-
No products found
because this supplier's products are not listed.
Subash Godar, et al.,
bioRxiv - Biophysics 2021
Quote:
... We detected the bound alkaline phosphatase labeled antibodies by incubating the membrane in BCIP/NBT (5-bromo, 4-chloro, 3- indolylphosphate/nitro-blue tetrazolium, AMRESCO, LLC.) substrate for 30–45 minutes ...
-
No products found
because this supplier's products are not listed.
Pazhanichamy Kalailingam, et al.,
bioRxiv - Pathology 2024
Quote:
... then incubated overnight at 37°C with staining buffer (5-bromo-4-chloro-3-indolyl β-D-galactopyranoside [X-gal, Bioline, London ...
-
No products found
because this supplier's products are not listed.
Esteban Flores, et al.,
bioRxiv - Microbiology 2023
Quote:
... 6 µg of 4:2:1:1 transfer:Gag-Pol (pMDLg-pRRE, Addgene):Rev (pRSV-Rev ...
-
No products found
because this supplier's products are not listed.
Agata Szuba, et al.,
bioRxiv - Cell Biology 2020
Quote:
... 5 mM dithiothreitol (DTT)) at 4°C overnight and concentrated to ~3-5 mg/mL using Vivaspin 6 concentrators (Sartorius, 6 ml, 100 kDa cut-off). Mammalian septin hexamers composed of mouse Sept2 (98.6% identical to human Sept2 ...
-
Cat# HY-111514-10 mg,
10 mg, USD $80.0
Ask
Aleksandra Tempes, et al.,
bioRxiv - Cell Biology 2023
Quote:
... cells were treated for 2 h with 60 µM 1-adamantyl(5- bromo-2-methoxybenzyl)amine (ABMA; Medchemexpress, catalog no. HY-124801) or 50 µM chloroquine (dissolved in water ...
-
No products found
because this supplier's products are not listed.
Kevin C. Courtney, et al.,
bioRxiv - Neuroscience 2021
Quote:
... and 1,2-dioleoyl-sn-glycero-3-phospho-(1′-myo-inositol-4′,5′- bisphosphate) (PI(4, 5)P2) were obtained from Avanti Polar Lipids. HEPES was from Fisher Scientific ...
-
No products found
because this supplier's products are not listed.
Agnieszka Lukaszewicz, et al.,
bioRxiv - Molecular Biology 2021
Quote:
... libraries from amplicons 1–4 were mixed with 5% PhiX DNA control library (Illumina) and sequenced ...
-
No products found
because this supplier's products are not listed.
Shulan Xiao, Saumitra Yadav, Krishna Jayant,
bioRxiv - Neuroscience 2022
Quote:
... 4 to 6 MΩ borosilicate patch pipette (Sutter Instruments, CA, USA) were pulled using a P1000 pipette puller (Sutter Instruments ...
-
No products found
because this supplier's products are not listed.
Imke L. Lemmer, et al.,
bioRxiv - Physiology 2023
Quote:
... Sections of 4-6 µm were produced using a microtome (Leica). Deparaffination and rehydration was performed using standard procedure by incubating in Xylene (2 x 5 min) ...
-
No products found
because this supplier's products are not listed.
LN Marziali, et al.,
bioRxiv - Neuroscience 2023
Quote:
... the cells were incubated with 4’,6-diamidino-2-phenylindol (DAPI; 1 μg/ml) and the corresponding Alexa Fluor-conjugated secondary antibodies (1:500; Jackson ImmunoResearch Laboratories) for 2 h at RT ...
-
No products found
because this supplier's products are not listed.
Lacey A Birnbaum, et al.,
bioRxiv - Bioengineering 2022
Quote:
... CD8+ T cells and 5×104 NALM-6 cells were plated with 0.5-1 ng/mL blinatumomab (Invivogen) and cytokine ...
-
Prepared to contain high collagenase activity with a caseinase to collagenase ratio of ~2:1....
Cat# LS005318,
100 mg, $60.00
Ask
Kai Guo, et al.,
bioRxiv - Immunology 2022
Quote:
... in DMEM containing 0.0002% L-1-(tosylamido-2-phenyl) ethyl chloromethyl ketone (TPCK)-treated trypsin (Worthington Biochemical) with antibiotics ...
-
No products found
because this supplier's products are not listed.
Chi-Yeh Chung, et al.,
bioRxiv - Genomics 2019
Quote:
... incubated for 5 min at 4 °C with rotation and pelleted again (500 x g, 5 min, 4°C; 5920R, Eppendorf). Nuclei were resuspended in 500 μL high salt tagmentation buffer (36.3 mM Tris-acetate (pH = 7.8) ...
-
No products found
because this supplier's products are not listed.
Andrew Barszczyk, et al.,
bioRxiv - Cell Biology 2023
Quote:
... alkaline phosphatase conjugated antibodies were detected colourometrically using BCIP/NBT (nitro-blue tetrazolium and 5- bromo-4-chloro-3’-indolyphosphate) Substrate Solution (#BE116.100ML; Bio Basic Canada Inc). For analysis of bands ...
-
No products found
because this supplier's products are not listed.
Ken Takashima, et al.,
bioRxiv - Cancer Biology 2023
Quote:
The long strand of the AsiC synthesized with 2′-fluoropyrimidines was annealed to the short antisense strand (TriLink Biotechnologies) with a 2-fold molar excess of the short strand ...
-
No products found
because this supplier's products are not listed.
Charles R. Heller, et al.,
bioRxiv - Neuroscience 2020
Quote:
... 1 - 4 tungsten micro-electrodes (FHC, 1-5 MΩ) were inserted to characterize the tuning and response latency of the region of cortex ...
-
No products found
because this supplier's products are not listed.
Sergii Palchevskyi, et al.,
bioRxiv - Molecular Biology 2022
Quote:
... 5-6 mM LMNG (Anatrace) and 1-1.2 mM Cholesteryl Hemisuccinate Tris Salt (CHS ...
-
No products found
because this supplier's products are not listed.
Amber Gonda, et al.,
bioRxiv - Cancer Biology 2021
Quote:
... Samples were lysed and incubated for 5 minutes in Trizol and then 1-bromo-3-chloropropane (BCP) (Molecular Research Center, Inc. Cincinnati, OH) was added to separate the RNA from the remaining material ...
-
No products found
because this supplier's products are not listed.
Peter Proks, et al.,
bioRxiv - Biophysics 2021
Quote:
... NFx and desamino chloro-fluoxetine (Toronto Research Chemicals). NFx was dissolved in DMSO and diluted to working concentrations on the day of experimenting (max final DMSO concentration was 0.3 %) ...
-
No products found
because this supplier's products are not listed.
Elizabeth B Wright, et al.,
bioRxiv - Neuroscience 2023
Quote:
... RNAseq data includes the following mice by age and sex: 4 5-6 month wild type (1F/3M), 3 5-6 month mutant (1F/2M) ...
-
No products found
because this supplier's products are not listed.
L Almeida, et al.,
bioRxiv - Immunology 2019
Quote:
... αIL-4 (5 μg mL−1, clone 11B11; Bio X Cell), rhTGF-β1 (2 ng mL−1 ...
-
No products found
because this supplier's products are not listed.
Naresh Kumar Dewangan, et al.,
bioRxiv - Microbiology 2023
Quote:
... Sections were incubated with 4′,6-diamidino-2-phenylindole (DAPI) (1:2000, Solarbio, Beijing, China) for 8 min in the dark ...
-
No products found
because this supplier's products are not listed.
Katarzyna M. Luda, et al.,
bioRxiv - Immunology 2022
Quote:
... and [13C4]-ethyl-acetoacetate (Cambridge Isotope labs) were hydrolysed by mixing 1ml of ethyl acetoacetate with 8 ml 1M NaOH at 60°C by stirring for 30 min ...
-
No products found
because this supplier's products are not listed.
Sebastiaan Pieter van Kessel, et al.,
bioRxiv - Microbiology 2021
Quote:
... Samples were analysed on a C18 column (Kinetex 5 μM, C18 100 Å, 250 × 4·6 mm, Phenomenex, Utrecht, The Netherlands) using a gradient of water/methanol with 0·1% formic acid (0–3 min ...
-
No products found
because this supplier's products are not listed.
Thivanka Muthumalage, et al.,
bioRxiv - Pharmacology and Toxicology 2020
Quote:
... and 6 hours using microplate spectrophotometer (Cytation 5, Biotek).
-
No products found
because this supplier's products are not listed.
Kailin Yin, et al.,
bioRxiv - Immunology 2023
Quote:
... 6 million cells were resuspended in 4 ml of PBS (Rockland) containing 2 mM EDTA (Corning ...
-
No products found
because this supplier's products are not listed.
Nikita Raj, et al.,
bioRxiv - Cell Biology 2022
Quote:
... 5-6 × 105 siRNA transfected or untransfected cells were cultured on collagen-coated 6-well plates (Ibidi, 80287) until 90-100 % confluency (20-24 hours) ...
-
No products found
because this supplier's products are not listed.
Eva Dervas, et al.,
bioRxiv - Pathology 2020
Quote:
... followed by a 15 min incubation with DAPI (4′, 6-diamidino-2-phenylindole, Novus Biologicals; 1:10,000 in PBS). Sections were washed twice with distilled water ...
-
No products found
because this supplier's products are not listed.
Conor J. Sugden, et al.,
bioRxiv - Cell Biology 2020
Quote:
1 × 10□ KERA-308 or 4 × 105 HEK293A cells were seeded in 6-well plates (Greiner-BioOne, Kremsmünster, Austria) 24 h prior to transfection ...
-
No products found
because this supplier's products are not listed.
Sophie Lanciano, et al.,
bioRxiv - Genomics 2023
Quote:
Two micrograms of genomic DNA were sonicated for 6 cycles (6 s on, 90 s off) at 4 °C with a Bioruptor NGS (Diagenode), generating average fragments of 1 kb ...
-
No products found
because this supplier's products are not listed.
Nina Jurčić, et al.,
bioRxiv - Neuroscience 2021
Quote:
... Patch pipettes (4-6 MΩ) were pulled from borosilicate glass capillaries (Harvard Apparatus) using a horizontal P-97 Flaming/Brown type micropipette puller (Sutter Instrument Co) ...
-
No products found
because this supplier's products are not listed.
Aidan B Estelle, et al.,
bioRxiv - Biophysics 2023
Quote:
... either labeled with 5’ 6-carboxyfluorescein or unlabeled and reverse complement 14mer (5’-GACGUCCAUGUGCC-3’) were purchased from GenScript. The dsRNA was prepared by annealing the ss-14mer (5’-GGCACAUGGACGUC-3’ ...
-
No products found
because this supplier's products are not listed.
James P Bridges, et al.,
bioRxiv - Cell Biology 2021
Quote:
... 5 % CO2 with 20 µl Calcium 6 loading buffer (Molecular Devices, Cat #R8191): 1x HBSS (Gibco ...
-
No products found
because this supplier's products are not listed.
Sergio Gil-Manso, et al.,
bioRxiv - Immunology 2021
Quote:
... A total of 1.5 × 106 PBMCs were stimulated with 1 µg/mL of peptide pools for 6 h in TexMACS™ GMP Medium (Miltenyi Biotec), supplemented with 5% AB human serum (Sigma-Aldrich ...
-
No products found
because this supplier's products are not listed.
Amanda L. Ziegler, et al.,
bioRxiv - Physiology 2022
Quote:
... 4- and 6- hours after scratch-wounding using an inverted microscope (Olympus IX81, Tokyo, Japan) with a digital camera (ORCA-flash 4.0 ...
-
No products found
because this supplier's products are not listed.
Bryan S. Sibert, et al.,
bioRxiv - Biophysics 2021
Quote:
To provide extra support for cell growth 5-6 nm of carbon was evaporated onto Quantifoil Au 200 R2/1 or R2/2 grids (Quantifoil Micro Tools GmbH). Grids were then glow discharged and incubated in supplemented RPMI-1640 overnight in the cell incubator ...
-
No products found
because this supplier's products are not listed.
Emmanouil D. Karagiannis, et al.,
bioRxiv - Bioengineering 2019
Quote:
... for 4-6 hours at room temperature and incubated in MAXbind Staining Medium (Active Motif, catalog no. 15251) containing primary antibodies at a concentration of 10 μg/mL overnight at 4□C ...
-
No products found
because this supplier's products are not listed.
Yiqiong Liu, et al.,
bioRxiv - Neuroscience 2024
Quote:
... for 1 day at 4 °C: rabbit anti-cFos (1:2000, Synaptic systems, #226003), chicken anti-GFP (1:2000 ...
-
No products found
because this supplier's products are not listed.
P. Lejeune, et al.,
bioRxiv - Plant Biology 2020
Quote:
... Jiffypots® with weak or abnormal plantlets were discarded and the others were transplanted into 12-cm square plastic cultivation pots filled with 1.5 L of leaf mould and baked clay (4:1) mixed with 6 gr.L−1 of slow release fertilizer (Osmocote Exact Standard 5-6 M, ICL Specialty Fertilizers). The pots were fitted at the bottom with a 2 x 10 cm felt wick and randomly placed on the deck of the cultivation gutters described above ...
-
No products found
because this supplier's products are not listed.
Annekatrien Boel, et al.,
bioRxiv - Genetics 2019
Quote:
... 1 hour and 4 hours of stimulation with TGFβ1 (4342-5, Biovision). Cells were scraped in PBS supplemented with protease inhibitor cocktail (1:100 ...
-
No products found
because this supplier's products are not listed.
Yoichi Araki, et al.,
bioRxiv - Neuroscience 2020
Quote:
... and time-lapse images were acquired with either LSM510 (Carl Zeiss; Fig. 1, 4, and 6) or Spinning disk confocal microscopes controlled by axiovision software (Carl Zeiss ...
-
6-Chloro-7-hydroxy-4-methylcoumarin is a pharmaceutical intermediate.
Cat# S5760, SKU# S5760-5mg,
5mg, $107.00
Ask
Liying Chen, et al.,
bioRxiv - Neuroscience 2023
Quote:
The D1 receptor antagonist R(+)-7-chloro-8-hydroxy-3-methyl-1-phenyl-2,3,4,5-tetrahydro-1H-3-benzazepi ne hydrochloride (SCH-23390, Selleck chemicals, China) was dissolved in 0.9% saline and stored -80°C ...
-
No products found
because this supplier's products are not listed.
Yue Qu, et al.,
bioRxiv - Neuroscience 2021
Quote:
The completed constructs in M-6-attB-UAS-1-3-4 vector were amplified in Epi300 competent cells (EpiCentre) in LB-Chloramphenicol medium ...
-
No products found
because this supplier's products are not listed.
He-Chin Hsieh, et al.,
bioRxiv - Microbiology 2023
Quote:
... and BA.4/5 (Genetex, Cat. No. GTX137098-pro) were mixed with RBD-LTA ...
-
No products found
because this supplier's products are not listed.
Jordan J. Aoyama, Medha Raina, Gisela Storz,
bioRxiv - Microbiology 2021
Quote:
... was separated on denaturing 8% polyacrylamide gels containing 6 M urea (1:4 mix of Ureagel Complete to Ureagel-8 (National Diagnostics) with 0.08% ammonium persulfate ...
-
No products found
because this supplier's products are not listed.
Yeon Soo Kim, et al.,
bioRxiv - Cancer Biology 2019
Quote:
... shSIRT3_#4: 5’-TCACATTCTGTTGACTCTCCATACTCAGC-3’) in pRS vector (Origene, TR309432) were used to stably transfect OVCA433 cells (Supp ...
-
No products found
because this supplier's products are not listed.
Alexander J. Stemm-Wolf, et al.,
bioRxiv - Cell Biology 2021
Quote:
... 1:2000 α-SAS-6 (Bethyl A301-802A); 1:500 α-CPAP (Proteintech CENPJ 11517-1-AP) ...
-
No products found
because this supplier's products are not listed.
Negm Ahmed, et al.,
bioRxiv - Neuroscience 2021
Quote:
... for 5 min at 4°C in Micro tube 1.1ml Z-Gel (Sarstedt, Germany). Samples were kept on ice at all times ...
-
No products found
because this supplier's products are not listed.
Jun Tominaga, et al.,
bioRxiv - Plant Biology 2020
Quote:
... a large 6 x 6 cm chamber (6800-13; LI-COR) was used to maximize measurement precision ...