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Citations for Sartorius :
1 - 50 of 353 citations for 6 1 BROMO ETHYL 4 CHLORO 5 FLUOROPYRIMIDINE since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2020Quote: ... 5 mM dithiothreitol (DTT)) at 4°C overnight and concentrated to ~3-5 mg/mL using Vivaspin 6 concentrators (Sartorius, 6 ml, 100 kDa cut-off). Mammalian septin hexamers composed of mouse Sept2 (98.6% identical to human Sept2 ...
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bioRxiv - Biophysics 2020Quote: ... Peak fractions containing purified Cx46/50 were pooled and concentrated to 5–6 mg mL-1 with a centrifugal device (Vivaspin 6; 50-kDa cut-off filter; Sartorius). Protein concentration was determined by UV absorbance at 280 nm ...
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bioRxiv - Cancer Biology 2021Quote: ... We imaged cells every 6 hours for 4-6 days of treatment using Incucyte Zoom (Sartorius, Michigan, USA). Fluorescence was quantified using four locations in each treated sample by the IncuCyte Zoom 2016B software.
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bioRxiv - Biochemistry 2021Quote: ... concentrated to 1 mL using a Vivaspin 4 Turbo concentrator (Cut-off 5 kDa; Sartorius), and purified via size-exclusion chromatography with a Superdex S75-10/300 column (GE Healthcare).
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bioRxiv - Neuroscience 2022Quote: ... The pooled fractions of the NOTCH3 EGF1-5 WT and R133C were concentrated to 1 mg/mL in a concentration column (Vivaspin 6 10 KDa MWCO, Sartorius) according to the manufacturer’s instructions ...
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bioRxiv - Neuroscience 2024Quote: ... Lysates were spun at 15,000g for 5 min at 4°C and supernatants were passed through a 4 mm regenerated cellulose filter (4 mm Ø, Sartorius). Samples were then dried under nitrogen flow at 40°C and resuspended in 125 µl of methanol/water 70:30 for subsequent analyses.
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bioRxiv - Microbiology 2019Quote: ... The eluted complex was then concentrated to 1 mL using a Vivaspin 4 Turbo concentrator (Cut-off 5 kDa; Sartorius). A final purification step was performed using size-exclusion chromatography by loading the proteins on a HiLoad 10/300 Superdex 200 column (GE Healthcare ...
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bioRxiv - Genetics 2023Quote: ... chip activation was made with a freshly prepared mixture of N-hydroxysuccinimide (50 mM in water) and 1-ethyl-3-(3-dimethylaminopropyl) carbodiimide (195 mM in water) for 7.5 min in DPBS (Sartorius, SKU 02-023-5A) (flow rate of 10 μL/min) ...
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bioRxiv - Molecular Biology 2021Quote: ... the volume was adjusted to 5 mL and transferred to a Centrifugal Concentrator (Vivaspin 6; Sartorius). The retentate was adjusted to 120 µL prior to analysis.
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bioRxiv - Microbiology 2023Quote: ... which took place overnight on arrival at 4°C (cold room) using 6 Vivaflow200 filtration units (Sartorius) operating in two parallel series ...
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bioRxiv - Bioengineering 2024Quote: ... After 24 hrs supernatant was collected and concentrated by the Vivaspin-6 5 kDa MWCO filters (Sartorius VS06S1). Cells were lysed in 20 mM Tris-HCl 7.4 pH at 4 °C for 1 hr ...
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bioRxiv - Biochemistry 2022Quote: ... and buffer was exchanged four times with 5 mL of 10 mM potassium phosphate buffer pH 7.4 using a Vivaspin 6 Centrifugal Concentrator (Sartorius) at 2,000 rcf and 4°C for 20 min ...
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bioRxiv - Immunology 2024Quote: ... Plates were imaged every 4-6 hours for 3-7 days using the IncuCyte® Live-Cell Analysis System (Sartorius). 5 images per well at 10x zoom were collected at each time point ...
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bioRxiv - Biochemistry 2020Quote: ... we concentrated the proteins from the supernate by membrane centrifugation using 5 K molecular weight cutoff Vivaspin units of 6 mL (Sartorius Stedim, Goettingen, Germany). The retentate (~300 μL ...
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bioRxiv - Biochemistry 2023Quote: ... Samples were incubated at room temperature during one hour and labeled proteins were separated from Ir2 and DMSO vehicle by 5 cycles of diafiltration (12,000 g, 4°C, molecular cutoff 10kDa, Sartorius) adding 5 volumes of labeling buffer before each cycle ...
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bioRxiv - Biochemistry 2023Quote: ... The resulting protein was concentrated to ≈4 mg/mL using a 5 kDa centrifugal concentrator (Vivaspin; Sartorius, Gottingen, Germany). For drop-deposition Raman spectroscopy ...
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bioRxiv - Biochemistry 2023Quote: ... every 4 h (10x objective, 5 fields per well) and were analysed using the Incucyte Live-Cell Analysis System (Sartorius) and are represented as RCU x µm²/Well.
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bioRxiv - Cell Biology 2022Quote: ... concentrated in 1 mL in a Vivaspin Turbo 4 (30’000 MWCO, Sartorius VS04T22) and further purified on a Superdex 75 10/300 gel filtration column (GE Healthcare).
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bioRxiv - Immunology 2020Quote: ... concentrated to 1-5 mg/ml by ultrafiltration using appropriate VivaSpin devices (Sartorius), were snap-frozen in liquid nitrogen in small aliquots and stored at -80°C.
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bioRxiv - Bioengineering 2024Quote: ... The purified protein from elutions containing the fewest contaminating proteins (typically elution steps 3-5) were concentrated in 1× PBS using a 5 kDa cut-off Vivaspin Centrifugal Concentrator (Sartorius, Oakville, Ontario, Canada). Protein concentrations were determined using the micro-BCA assay (Thermo Fisher Scientific ...
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bioRxiv - Microbiology 2023Quote: ... After concentration (Vivaspin 6 Concentrator, Sartorius Stedim Biotech) and desalting (PD 10 Desalting Columns ...
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bioRxiv - Molecular Biology 2022Quote: ... concentrated with a Vivaspin 6 (10 kDa MWCO) (Sartorius), flash-frozen by liquid nitrogen ...
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bioRxiv - Biochemistry 2023Quote: ... concentrated to 5 mg mL-1 using a 100 kDa molecular weight cut off concentrator (Sartorius), and processed immediately for cryo-electron microscopy.
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bioRxiv - Neuroscience 2023Quote: ... The eluent was concentrated using Vivaspin 6 Centrifugal Concentrators (Sartorius). Imidazole was removed from the concentrated eluent using PD MiniTrap Sample Preparation Columns (Cytiva ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... The eluent was concentrated using Vivaspin 6 Centrifugal Concentrators (Sartorius). Imidazole was removed from the concentrated eluent using PD MiniTrap Sample Preparation Columns (Cytiva ...
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bioRxiv - Microbiology 2020Quote: ... using the Vivaspin 6 centrifugal concentrator (MWCO 1,000,000 kDa) (Sartorius, Australia), was required for high-quality electron microscopy visualization ...
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bioRxiv - Microbiology 2020Quote: ... and then concentrated using Vivaspin® 6 polyethersulfone 10 kDa (Sartorius). The purified and concentrated protein was delivered to GeneScript-Nanjing to raise the anti-rabbit polyclonal antibody.
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bioRxiv - Microbiology 2020Quote: ... and then concentrated using Vivaspin® 6 polyethersulfone 10 kDa (Sartorius). Concentrations of the purified proteins were measured using BCA protein assay kit (Beyotime ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... The eluted complex was concentrated using Vivaspin 6 Centrifugal Concentrators (Sartorius), and imidazole was removed from the protein solution by applying the sample to a PD MiniTrap Sample Column (Cytiva ...
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bioRxiv - Immunology 2024Quote: ... The collagen suspension was mixed carefully with the cells in a ratio of 4:1 and 50 µl were added in a 96 well image lock plate (Sartorius) on ice ...
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bioRxiv - Cell Biology 2021Quote: ... concentrated to 300-350 µl using Vivaspin 6 30,000 MWCO concentrators (Sartorius), and loaded onto a Superdex 200 10/300 GL column equilibrated in SEC Buffer (25 mM Tris pH 8.0 ...
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bioRxiv - Plant Biology 2022Quote: ... then concentrated using Vivaspin® 6 concentrators (Sartorius Stedim Lab Ltd, UK) and stored at -80°C before use.
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bioRxiv - Plant Biology 2022Quote: ... then concentrated using Vivaspin® 6 concentrators (Sartorius Stedim Lab Ltd, UK) and stored at -80 °C before use.
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bioRxiv - Biochemistry 2023Quote: ... Dialysed protein was spin-concentrated in a Vivaspin 6 MWCO 50,000 (Sartorius) to the desired concentration ...
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bioRxiv - Systems Biology 2023Quote: ... followed by live-cell imaging every 6 h using Incucyte Systems (Sartorius) for the duration of 1 week ...
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bioRxiv - Biochemistry 2020Quote: ... Two 5 L bioreactors (Sartorius) were seeded at 0.3 x 106 cells/mL in 3 L (total working volume ...
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bioRxiv - Microbiology 2022Quote: ... 5 mM MgCl2) and concentrated to ~1 mg/mL by Vivaspin 20 centrifugal filter unit (MWCO 10 kDa) (Sartorius). Sfp synthase and CoA-conjugated dyes were prepared as previously described54 and used to perform the overnight Fab labeling reaction on ice ...
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bioRxiv - Bioengineering 2020Quote: ... GO16 KS3 was cultivated in a 5 L stirred tank bioreactor (Sartorius UniVessel Glass 5 l) containing 3 L of MSMlim broth ...
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bioRxiv - Biochemistry 2020Quote: ... or Vivaspin (Sartorius, MWCO 5 kDa) concentration columns.
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bioRxiv - Neuroscience 2023Quote: ... The supernatant was concentrated by centrifugation for 1 hour at 3220 g at 4°C in a 5kDa Cutoff Vivaspin tube (Sartorius, Cat # VS2012). ACM was aliquoted into low protein binding tubes (∼100ul per aliquot) ...
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bioRxiv - Bioengineering 2023Quote: Sylgard-184™ silicone elastomer kit PDMS pre-polymer was well mixed (5 mins) with curing agent at a 10:1 w/w ratio using a digital balance (Sartorius BP610) prior to vacuum desiccation and casting at various temperatures (60-90°C).
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bioRxiv - Immunology 2023Quote: ... the mNeonGreen signals from infected MRC-5 cells were monitored for 1 week using the Incucyte™ Live-Cell analysis system (Sartorius). Additionally ...
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bioRxiv - Molecular Biology 2022Quote: ... Subsamples were weighed (20 mg ± 6 mg) on a fine scale balance (Entris, Sartorius). Together with 6 negative controls (200 μl lysis buffer ...
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bioRxiv - Microbiology 2023Quote: ... the samples were concentrated using filter columns (Vivaspin 6, 100,000 MWCO PES, Sartorius, Germany) at 4 °C with a maximal speed of 4,000 x g and washed twice with DBCM2 salt solution ...
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bioRxiv - Cell Biology 2019Quote: ... 4°C in Vivaspin20 columns (Cat.#VS2032,Sartorius) and stored at –80°C.
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bioRxiv - Immunology 2019Quote: ... Worm culture supernatants collected over 1 week were further concentrated using centrifugal protein concentrators with a 5 kDa MWCO (Vivaspin, Sartorius, Göttingen, Germany) to obtain the final ...
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bioRxiv - Plant Biology 2021Quote: ... and 5-μm syringe filter (Sartorius, Germany) to remove RLX (0 ...
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bioRxiv - Microbiology 2020Quote: ... The resulting supernatant was then concentrated using Vivaspin® 6 100,000 MWCO concentrator columns (Sartorius). The sample was then purified via size exclusion chromatography using a Superose™ 6 Increase column (GE Life Sciences ...
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bioRxiv - Microbiology 2022Quote: ... The aliquots containing the purified His-ChuP were concentrated using a VivaSpin 6 column (Sartorius), and desalted by gel filtration in PD-10 column (GE Healthcare ...
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bioRxiv - Microbiology 2020Quote: ... Samples containing purified protein were concentrated using Vivaspin® 6 5,000 MWCO columns (Sartorius Stedim). Protein was buffer exchanged using membrane filtration into 50 mM NaP ...