-
No products found
because this supplier's products are not listed.
Ashley N. Barlev, et al.,
bioRxiv - Immunology 2021
Quote:
... or equimolar concentrations of the Fab’2 fragments (Jackson Immunoresearch Affinipure; 0.4-50 ug/mL) for 20 hours after which activation was measured using flow cytometry as described above.
-
No products found
because this supplier's products are not listed.
Dillon S. McDevitt, et al.,
bioRxiv - Neuroscience 2019
Quote:
... recording electrodes (2-5 MΩ; borosilicate glass capillaries (WPI #1B150F-4) pulled on a horizontal puller from Sutter Instruments (model P-97) ...
-
No products found
because this supplier's products are not listed.
Mario Mietzsch, et al.,
bioRxiv - Molecular Biology 2021
Quote:
... 3.5 μL was applied to a glow-discharged Quantifoil copper grid with 2 nm continuous carbon support over holes (Quantifoil R 2/4 400 mesh), blotted ...
-
No products found
because this supplier's products are not listed.
Sonu Shrestha Baral, et al.,
bioRxiv - Cell Biology 2019
Quote:
... Tissues were counter-stained with 4’,6-diamino-2-phenylindole (DAPI, Polysciences) at 1 μg/mL ...
-
No products found
because this supplier's products are not listed.
Dong Wang, et al.,
bioRxiv - Cancer Biology 2024
Quote:
... stained with spectral 4′,6-diamidino-2-phenylindole (Perkin Elmer), and coverslipped with ProLong Diamond mounting media (Thermo Fisher) ...
-
No products found
because this supplier's products are not listed.
Ingie Hong, et al.,
bioRxiv - Neuroscience 2023
Quote:
... We filled patch pipettes (2-4 MΩ) pulled (P-97, Sutter Instrument) from borosilicate capillary glass (Sutter Instrument ...
-
No products found
because this supplier's products are not listed.
Niccolò Paolo Pampaloni, et al.,
bioRxiv - Neuroscience 2020
Quote:
... Slices were superfused with recirculating aCSF (5 mL/min at room temperature) containing 4-methoxy-7-nitroindolinyl-glutamate (MNI-caged-L-Glutamate; HelloBio HB0423) at a concentration of 0.5 mM and used for the whole day of recordings.
-
No products found
because this supplier's products are not listed.
Maria Magdalena Klicznik, et al.,
bioRxiv - Immunology 2019
Quote:
... 130-045-101) and 4×106 cells/ml were resuspended in RPMIc with 50 U/ml IL-2 (Immunotools; 11340023) in a 24-well and stimulated for 20h with 25μl/ml ImmunoCult CD3/CD28 T cell activator (Stemcell ...
-
No products found
because this supplier's products are not listed.
Sarah Hofmann, et al.,
bioRxiv - Cancer Biology 2021
Quote:
... 5 ug/ml insulin (PromoCell), 0.5 ug/ml Hydrocortisone ...
-
No products found
because this supplier's products are not listed.
Lucas Bayonés, et al.,
bioRxiv - Neuroscience 2024
Quote:
... stained with 5 mg/ml 4′,6-diamidino-2-phenylindole and visualized using a Nikon C1 Plus laser-scanning confocal microscope (Nikon, Tokyo, Japan). All images were captured at the equatorial plane of the cells ...
-
No products found
because this supplier's products are not listed.
Janne J.M. van Schie, et al.,
bioRxiv - Molecular Biology 2022
Quote:
... 2 mg of chromatin lysate was rotated overnight at 4°C with 5 μg antibody: rabbit IgG (Diagenode #C15410206), anti-NIPBL (NIPBL#1 ...
-
No products found
because this supplier's products are not listed.
Pawel Mikulski, et al.,
bioRxiv - Molecular Biology 2023
Quote:
... 2 or 4 nM concentration and run on NextSeq 550 sequencer (Illumina) with NextSeq 500/550 High Output v2.5 (75 cycles PE ...
-
No products found
because this supplier's products are not listed.
Amélie Bauer, et al.,
bioRxiv - Plant Biology 2023
Quote:
... except for the seed compressions at 2 DPA (Fig 4) where a SP8 confocal microscope (Leica Microsystems) with a 25x long distance water-dipping objective (NA=0.95 ...
-
No products found
because this supplier's products are not listed.
Paul Batty, et al.,
bioRxiv - Cell Biology 2023
Quote:
... 2 mM 4-thiothymidine (4sT) (Carbosynth, NT06341) was also supplemented to the medium at this stage ...
-
No products found
because this supplier's products are not listed.
Óscar Aguilar-Sopeña, et al.,
bioRxiv - Immunology 2023
Quote:
... and IL-2 (50 U/mL, from Proteintech, Germany) for 4 days ...
-
No products found
because this supplier's products are not listed.
Salman Tamaddon-Jahromi, et al.,
bioRxiv - Cell Biology 2023
Quote:
SKOV-3 cells transfected without or with 100nM siRNA and simultaneously treated with 10μM 5-Aza-Cdr for 4 days were seeded into 2-well cell culture inserts (Ibidi, UK). After cell attachment (6h) ...
-
No products found
because this supplier's products are not listed.
Biying Sun, et al.,
bioRxiv - Plant Biology 2024
Quote:
... 1 mL of supernatant was incubated at 4°C for 2 h with GFP-Trap_A beads (Chromotek, Planegg-Martinsried, Germany). The beads were collected by centrifugation at 800 × g and washed four times in 1 mL washing buffer (25 mM Tris pH=7.5 ...
-
No products found
because this supplier's products are not listed.
Odile Fabre, et al.,
bioRxiv - Cell Biology 2020
Quote:
... 2 mM 5-aminoimidazole-4-carboxamide ribonucleotide (AICAR, Toronto Research Chemicals), or 500 ng/mL mouse recombinant Gremlin 1 (R&D Biosciences ...
-
No products found
because this supplier's products are not listed.
Christopher D. Greer, et al.,
bioRxiv - Immunology 2021
Quote:
... IL-4 (MABTECH, 3311-4APW-2), and IL-10 (MABTECH ...
-
No products found
because this supplier's products are not listed.
Yang Liu, et al.,
bioRxiv - Cancer Biology 2024
Quote:
... and incubated with 4′,6-diamidino-2-phenylindole (Solarbio, #C0065) for 10 min ...
-
No products found
because this supplier's products are not listed.
S. Bayraktar, et al.,
bioRxiv - Cell Biology 2019
Quote:
... 2×104 cells/ml were seeded into 4-well or 8-well glass-bottomed dishes (Sarstedt) and incubated for 24 h ...
-
No products found
because this supplier's products are not listed.
M Perez-Zabalza, et al.,
bioRxiv - Neuroscience 2019
Quote:
Extracellular multiunit recordings were obtained with 2–4 MΩ tungsten electrodes (FHC, Bowdoinham, ME). Multiunit activity (MUA ...
-
No products found
because this supplier's products are not listed.
Ethan A. Older, et al.,
bioRxiv - Biochemistry 2023
Quote:
... using 50 nM hMD-2 (Novus Biologicals), 1 ng/mL LPS-EB Biotin (Invivogen) ...
-
No products found
because this supplier's products are not listed.
Diego A. Guzmán, et al.,
bioRxiv - Microbiology 2023
Quote:
... 4 ml of urine was added to 4 ml of 2X DNA/RNA Shield® (Zymo, USA). Serum and urine samples were stored and transported on ice to the Institute of Microbiology at Universidad San Francisco de Quito and thereafter maintained at −20°C until DNA extraction.
-
No products found
because this supplier's products are not listed.
Silian Chen, et al.,
bioRxiv - Biochemistry 2020
Quote:
... PBS containing 2 μg/mL Hoechst 33342 and 4 μg/mL pyronin Y (Amresco, cat# 0207) was added to the cells to stain DNA and RNA ...
-
No products found
because this supplier's products are not listed.
John C. Obenauer, et al.,
bioRxiv - Neuroscience 2023
Quote:
The proteomics signature had 4 validation data sets: R6/2 mice aged 2 months (R6/2 2M) or 3 months (R6/2 3M) from PRIDE experiment PXD013771 ...
-
No products found
because this supplier's products are not listed.
Soeren Lukassen, et al.,
bioRxiv - Genomics 2020
Quote:
... Up to 4 pieces were distributed in 2 ml cyro vials (Greiner Bio-One GmbH). Thereafter ...
-
No products found
because this supplier's products are not listed.
Jeroen van den Berg, et al.,
bioRxiv - Cell Biology 2019
Quote:
... a Lionheart FX automated microscope in combination with sirDNA77 staining was used to generate growth curves with a time resolution of 4 hours for a total time span of 136 hours (microscope maintained at 37°C, 5% CO2 using a 4× lens and a Sony CCD, 1.25 megapixel camera with 2 times binning; BioTek). Quantification of cell number was performed by Gen5 software (BioTek).
-
No products found
because this supplier's products are not listed.
Wenling Chen, Juan Carlos Marvizon,
bioRxiv - Neuroscience 2020
Quote:
Male adult (2-4 months old) Sprague-Dawley rats (Envigo, Indianapolis, IN) were used in the study ...
-
No products found
because this supplier's products are not listed.
Amelia Trimarco, et al.,
bioRxiv - Neuroscience 2024
Quote:
... Lysates were spun at 15,000g for 5 min at 4°C and supernatants were passed through a 4 mm regenerated cellulose filter (4 mm Ø, Sartorius). Samples were then dried under nitrogen flow at 40°C and resuspended in 125 µl of methanol/water 70:30 for subsequent analyses.
-
No products found
because this supplier's products are not listed.
SJ Gosai, et al.,
bioRxiv - Synthetic Biology 2023
Quote:
... Injected embryos were imaged at the indicated days (2 or 4 days-post-fertilization) either by dissecting (Olympus) or confocal fluorescence (Leica SP6 ...
-
No products found
because this supplier's products are not listed.
Olivier Da Ines, et al.,
bioRxiv - Genetics 2022
Quote:
... 2 mM X-Gluc (5-bromo-4-chloro-3-indolyl-ß-D-glucuronic acid; Biosynth), dissolved in N,N-dimethylformamide) ...
-
No products found
because this supplier's products are not listed.
Stephanie N. Caty, et al.,
bioRxiv - Microbiology 2024
Quote:
... 100 µL of each strain was transferred from the unlabeled glucose M9 media to 5 mL of M9 media with 50% 13C glucose (99% 13C, Cambridge Isotope Laboratory) and 50% natural abundance glucose and allowed to grow overnight at room temperature ...
-
No products found
because this supplier's products are not listed.
Lieke Koornneef, et al.,
bioRxiv - Developmental Biology 2022
Quote:
Localizations were drift corrected (model-based approach) and grouped (max on time = 50 frames, off gap = 2 frames, capture radius = 4 pixels) using ZEN2012 software (Carl Zeiss). We used the fluorescent 100 nm fiducials (Thermo Fisher ...
-
No products found
because this supplier's products are not listed.
David Jonathan Wasilko, et al.,
bioRxiv - Biochemistry 2024
Quote:
... The resuspension was solubilized at 4 °C for 4 h with 2% (w/v) Lauryl Maltose Neopentyl Glycol (LMNG, Anatrace) and 0.2% (w/v ...
-
No products found
because this supplier's products are not listed.
Nicole DelRosso, et al.,
bioRxiv - Systems Biology 2022
Quote:
... 2 ul per tube was transformed into two tubes of 50 ml of Endura electrocompetent cells (Lucigen, Cat#60242-2) following the manufacturer’s instructions ...
-
No products found
because this supplier's products are not listed.
Valerie Siahaan, et al.,
bioRxiv - Biophysics 2021
Quote:
... were polymerized from 4 mg/ml tubulin for 2 h at 37 °C in BRB80 supplemented with 1mM MgCl2 and 1mM GMPCPP (Jena Bioscience, NU-405). The polymerized microtubules were centrifuged for 30 min at 18000 x g in a Microfuge 18 Centrifuge (Beckman Coulter) ...
-
No products found
because this supplier's products are not listed.
Alexis M Ceasrine, et al.,
bioRxiv - Neuroscience 2022
Quote:
... then incubated for 2 nights at 4°C with chicken anti-Iba1 (Synaptic Systems, 234 006) or goat anti-Iba1 (Novus ...
-
No products found
because this supplier's products are not listed.
Daniel da Silva e Silva, et al.,
bioRxiv - Neuroscience 2022
Quote:
... post-fixed for 2 h in 4% PFA at 4°C and immediately sectioned in coronal slices (50 μm) using a vibratome (Ted Pella). Coronal sections were incubated with 10 mM sodium citrate (pH 6.0 ...
-
No products found
because this supplier's products are not listed.
Helmut Bischof, et al.,
bioRxiv - Cancer Biology 2023
Quote:
Glucose uptake was assessed using 2-(N-(7-Nitrobenz-2-oxa-1,3-diazol-4-yl)Amino)-2-Deoxyglucose (2- NBDG) (Biomol GmbH). Therefore ...
-
No products found
because this supplier's products are not listed.
Carly R. Mickelson, et al.,
bioRxiv - Neuroscience 2022
Quote:
... mechanically ventilated (2-4 mL tidal volume; VentElite Small Animal Ventilator, Harvard Apparatus, Holliston, MA, USA), and bilaterally vagotomized to prevent mechanosensory feedback from ventilation ...
-
No products found
because this supplier's products are not listed.
Hui Zhang, et al.,
bioRxiv - Microbiology 2021
Quote:
One ug/ml His-tagged spike RBD protein of SARS-CoV-2 (SPD-C52H3, Acrobiosystems) were immobilized onto 96-well plates (9018 ...
-
No products found
because this supplier's products are not listed.
Kazuhiro Hayashi, et al.,
bioRxiv - Neuroscience 2023
Quote:
... 25mM 4-(2-hydroxyethyl)-1-piperazineethanesulfonic acid (HEPES)(Research Products International) or acidic pH 6.5 media (26mM NaHCO3 ...
-
No products found
because this supplier's products are not listed.
Maria Zimmermann-Kogadeeva, et al.,
bioRxiv - Systems Biology 2022
Quote:
... and lysed in a bead beater high for 4 min (2 min / kept on ice 1 min / 2 min) (BioSpec Products). Cellular debris was removed by centrifugation (8,000 × g ...
-
No products found
because this supplier's products are not listed.
Erin A. Akins, et al.,
bioRxiv - Bioengineering 2023
Quote:
... Microglia were polarized to M2 with 50 ng/ml interleukin-4 (IL-4, Bio Basic, RC212-15-5) for 48 hours.
-
No products found
because this supplier's products are not listed.
Megan E. Conway, et al.,
bioRxiv - Cancer Biology 2019
Quote:
... 50 ug/ml Gentamicin (Genesee 25-533), 10 uM Y-27632 (Selleckchem S1049) ...
-
No products found
because this supplier's products are not listed.
Trinovita Andraini, et al.,
bioRxiv - Neuroscience 2021
Quote:
... 3-(2,4-Dichloro-5-methoxyphenyl)-2-sulfanyl-4(3H)-quinazolinone (BML-CM127-0050, Enzo Life Sciences) was injected at 20mg/kg (in 10% DMSO ...
-
No products found
because this supplier's products are not listed.
Abhichart Krissanaprasit, et al.,
bioRxiv - Bioengineering 2020
Quote:
... 2’-Fluoro-2’-deoxycytidine-5’-Triphosphate (2’F-dCTP) and 2’-Fluoro-2’-deoxyuridine-5’-Triphosphate (2’F-dUTP) were purchased from Trilink Biotechnologies ...
-
No products found
because this supplier's products are not listed.
Michihito Sasaki, et al.,
bioRxiv - Microbiology 2022
Quote:
Calu-3 cells were inoculated with SARS-CoV-2 Delta variant at an MOI of 3 for 2 h in M), camostat (50 μM, Wako) or anti-SARS-CoV-2 spike (1 μg/ml, GTX635792, GeneTex)] ...
-
No products found
because this supplier's products are not listed.
Davide Gerna, et al.,
bioRxiv - Plant Biology 2020
Quote:
... LMW carbonyls were separated using a reversed-phase column (NUCLEODUR C18 Pyramid, EC 50/2, 50×2 mm, 1.8 μm, Macherey-Nagel, Düren, Germany), using an Ekspert ultraLC 100 UHPLC system (AB SCIEX ...