-
No products found
because this supplier's products are not listed.
Diede de Haan, et al.,
bioRxiv - Cell Biology 2021
Quote:
... PDMPO [2-(4-pyridyl)-5-((4-(2-dimethylaminoethyl-amino-carbamoyl)methoxy)-phenyl)oxazole] (ThermoFisher Scientific, USA) was added to a final concentration of 330 µM ...
-
No products found
because this supplier's products are not listed.
Rohit Singh, et al.,
bioRxiv - Pharmacology and Toxicology 2024
Quote:
... The addition of alanine-4-methoxy-2-naphthylamide (Ala-4-MNA) substrate (Sigma) at a final concentration of 190 µM starts the reaction ...
-
No products found
because this supplier's products are not listed.
Christof Gaunt, et al.,
bioRxiv - Immunology 2021
Quote:
... anti-IL-4 antibody (2 ug/ml; clone MD-1, Biolegend), bexarotene (1 μM ...
-
No products found
because this supplier's products are not listed.
Ojasee Bapat, et al.,
bioRxiv - Neuroscience 2023
Quote:
... and 2 mM 4-Methoxy-7-nitroindolinyl-caged-L-glutamate (MNI caged glutamate) (Tocris Bioscience ...
-
No products found
because this supplier's products are not listed.
Lyne Khair, et al.,
bioRxiv - Cancer Biology 2023
Quote:
... and 2 ug/ml IL-6 (Peprotech). To quantify oxidative stress ...
-
No products found
because this supplier's products are not listed.
Karen L. Xu, et al.,
bioRxiv - Bioengineering 2023
Quote:
... Ki67 antibodies (2 ug/mL, Abcam ab15580) were added in 3% BSA to the sections and incubated at 4°C overnight ...
-
No products found
because this supplier's products are not listed.
Maali AlAhmad, et al.,
bioRxiv - Cell Biology 2024
Quote:
... 4-hydroxy-3-methoxy-acetophenone (Apocynin, 73536, Merck), gp-91-ds-tat (AS-63818 ...
-
No products found
because this supplier's products are not listed.
Antonia C. Darragh, Scott A. Rifkin,
bioRxiv - Evolutionary Biology 2022
Quote:
... Embryos were then washed with wash buffer and their nuclei stained with 5 ug/mL DAPI (4′,6-diamidino-2-phenylindole, Roche) prepared in wash buffer for 10 minutes at 30 ºC.
-
No products found
because this supplier's products are not listed.
Nitin Wadhwani, et al.,
bioRxiv - Cancer Biology 2022
Quote:
SJ-GBM2 and SF8628 cells were used to determine if reducing WDR82 through inducible knockdown affected cell viability 1×104 cells/100μl were plated in 96-well plates with complete cell culture medium with or without Dox (2μg/ml), and subjected to 3-(4, 5-dimethylthiazol-2-yl)-5-(3-carboxymethoxyphenyl)-2-(4-sulfophenyl)-2H-tetrazolium (MTS, Promega) assay.
-
No products found
because this supplier's products are not listed.
Eszter Somogyi, et al.,
bioRxiv - Immunology 2020
Quote:
... the media were refreshed and supplemented with 5 ng/mL IL-7 or 5 ng/mL IL-7 and 4 ng/ml IL-2 (R&D Systems), respectively ...
-
No products found
because this supplier's products are not listed.
Pattama Wiriyasermkul, et al.,
bioRxiv - Biochemistry 2023
Quote:
... 50 μL of 1 mg/mL XTT (2,3-Bis-(2-Methoxy-4-Nitro-5-Sulfophenyl)-2H-Tetrazolium-5-Carboxanilide) (Biotium) was mixed with 5 μL of 1.5 mg/mL phenazine methosulfate ...
-
No products found
because this supplier's products are not listed.
EL Castranio, et al.,
bioRxiv - Neuroscience 2022
Quote:
... 50% 5 μM methoxy-XO4 (Cayman Chemical) in 50% EtOH for 2 minutes ...
-
No products found
because this supplier's products are not listed.
Kim S. ROBINSON, et al.,
bioRxiv - Immunology 2023
Quote:
... 2 ml of collagen I (4 mg/ml) (Corning, #354249) mixed with 7.5×105 human fibroblasts were allowed to polymerize over 1 ml of acellular collagen I in six-well culture inserts (Falcon ...
-
No products found
because this supplier's products are not listed.
Suhas Sureshchandra, et al.,
bioRxiv - Immunology 2022
Quote:
... 2 ug/mL Pam3CSK4 (TLR1/2 agonist, Invivogen), and 1 ug/mL FSL-1 (TLR2/6 agonist ...
-
No products found
because this supplier's products are not listed.
Joshua D. Kerkaert, et al.,
bioRxiv - Microbiology 2021
Quote:
... and 300μL of XTT solution was added to each well (0.5mg/mL XTT [2,3-bis-(2-methoxy-4-nitro-5-sulfophenyl)-2H-tetrazolium-5-carboxanilide] (VWR) with 25μM menadione in PBS) ...
-
No products found
because this supplier's products are not listed.
Hsiao-Wei Tsao, et al.,
bioRxiv - Immunology 2021
Quote:
... 50 U/ml Penicillin and 50 ug/ml Streptomycin) supplemented with 100 U/ml hIL-2 and 2 ug/ml anti-CD28 (BD Pharmingen, 553294). Two days after the primary stimulation ...
-
No products found
because this supplier's products are not listed.
Tanya Puccio, et al.,
bioRxiv - Microbiology 2020
Quote:
... 2 mL of fermentor culture was added to 4 mL RNAprotect Bacteria Reagent (Qiagen) and immediately vortexed for 10 sec ...
-
No products found
because this supplier's products are not listed.
Keith F. DeLuca, et al.,
bioRxiv - Cell Biology 2021
Quote:
... 2 ml bed volume (0.8 × 4 cm) empty polypropylene column (BioRad) fitted with a two-way stopcock up to the fill line ...
-
No products found
because this supplier's products are not listed.
Robin Burns, et al.,
bioRxiv - Evolutionary Biology 2021
Quote:
... Chromosomes were counterstained with DAPI (4’,6-diamidino-2-phenylindole; 2 μg/ml) in Vectashield (Vector Laboratories). Fluorescent signals were analyzed and photographed using a Zeiss Axioimager epifluorescence microscope and a CoolCube camera (MetaSystems) ...
-
No products found
because this supplier's products are not listed.
Rohan Kodgule, et al.,
bioRxiv - Cancer Biology 2022
Quote:
... and rotated at 4°C overnight with 2-5 ug of antibody (H3K27ac, Active Motif Cat #39133; V5 tag, ThermoFisher #R960-25; ETV6, Santa Cruz, # sc-166835x ...
-
No products found
because this supplier's products are not listed.
Ruthellen H. Anderson, et al.,
bioRxiv - Cell Biology 2021
Quote:
... 1.5 in needle containing 2 mL 4% PFA (Electron Microscopy Sciences) diluted in stabilization buffer ...
-
No products found
because this supplier's products are not listed.
Boris Bonaventure, et al.,
bioRxiv - Microbiology 2021
Quote:
... and 50 U/mL interleukin-2 (IL-2, Miltenyi Biotec) prior to electroporation.
-
No products found
because this supplier's products are not listed.
James Chen, et al.,
bioRxiv - Biochemistry 2022
Quote:
... #DF0446-07-5) or 7H10 (Difco, cat# DF0627-17-4) plates containing 50 ug/mL hygromycin (GoldBio, cat. #H-270) and incubated at 37° C for 3-5 days ...
-
No products found
because this supplier's products are not listed.
Rayan Khaddaj-Mallat, et al.,
bioRxiv - Neuroscience 2021
Quote:
Pericytes viability was assessed using the 2,3-bis-(2-methoxy-4-nitro-5-sulfophenyl)-2H-tetrazolium-5-carboxanilide (XTT) assay according to the manufacturer’s procedure (Cell Signaling Technology). Cells were plated at the appropriate concentration in 96-well plates under different experimental conditions ...
-
Prepared to contain lower tryptic activity levels to limit damage to membrane proteins and...
Cat# LS004191,
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Chunlei Cang, Boxun Lu, Dejian Ren,
bioRxiv - Physiology 2020
Quote:
... 4 mg collagenase type 2 (Worthington) and 1.5 mg trypsin (Worthington) ...
-
No products found
because this supplier's products are not listed.
Sora Kim, et al.,
bioRxiv - Biophysics 2022
Quote:
... were mixed in 70 μL of assembly buffer (50 mM HEPES-KOH, pH 7.5, 300 mM KCl, 5 mM MgCl2, 2 mM TCEP, 4% glycerol, 2 mM ATPγS [Calbiochem]) for 5 min at 25 °C ...
-
No products found
because this supplier's products are not listed.
Lixia Wang, et al.,
bioRxiv - Immunology 2023
Quote:
... 4 μM oligomycin and 50 mM 2-DG (Agilent, Cat# 103020-100).
-
No products found
because this supplier's products are not listed.
Ying-Chao Hsueh, et al.,
bioRxiv - Immunology 2022
Quote:
... 5 ug/ml Brefeldin A was supplemented 4 hrs before subsequent Fc blocking (2.4G2, BioXCell), surface antibody staining ...
-
No products found
because this supplier's products are not listed.
Mareike D. Hoffmann, et al.,
bioRxiv - Synthetic Biology 2019
Quote:
... 5 μl of the resulting amplicon were diluted 1:4 in 1x buffer 2 (NEB), followed by denaturation and re-annealing in a nexus GSX1 Mastercycler (Eppendorf ...
-
No products found
because this supplier's products are not listed.
Shabnam Mohammadi, et al.,
bioRxiv - Evolutionary Biology 2022
Quote:
... Sf9 cells (4 × 105 cells* ml) in 2 ml of Insect-Xpress medium (Lonza, Walkersville, MD, USA) were transfected with recombinant bacmids using Cellfectin reagent (Life Technologies) ...
-
No products found
because this supplier's products are not listed.
Christopher D. Greer, et al.,
bioRxiv - Immunology 2021
Quote:
... were coated overnight at 4°C with either 2 ug/ml SARS-CoV-2 Spike Protein ECD (Sino Biological, 40589-V08B1) or 1 ug/ml SARS-CoV-2 Spike-RBD (Sino Biological ...
-
No products found
because this supplier's products are not listed.
Jessica D. Resnick, et al.,
bioRxiv - Microbiology 2023
Quote:
... and 4 ug/mL Heparin sodium salt in PBS (StemCell Technologies) was replaced on the basolateral side only ...
-
No products found
because this supplier's products are not listed.
Priya Rani, et al.,
bioRxiv - Molecular Biology 2023
Quote:
... 1-1.5 μg of total protein was loaded onto a 10% to 50% sucrose gradient and centrifuged at 36,000 rpm for 2 hours at 4°C using an SW41 rotor (Beckman). Polysome profiles were visualized ...
-
No products found
because this supplier's products are not listed.
Chris H. Hill, et al.,
bioRxiv - Molecular Biology 2021
Quote:
... Supernatant was incubated (2 h, 4 °C) with 4.0 mL of Glutathione Sepharose 4B resin (GE Healthcare) that had been pre-equilibrated in the same buffer ...
-
Cat# HY-W019940,
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Guohui Zhang, et al.,
bioRxiv - Biophysics 2022
Quote:
BC5 (ARG-4-Methoxy-2-Naphthylamine, ordered from Medchemexpress LLC Monmouth Junction, NJ) was dissolved into DMSO to make 100 mM stock solution and then added to recording solutions to the final concentrations ...
-
No products found
because this supplier's products are not listed.
Rohan Kodgule, et al.,
bioRxiv - Cancer Biology 2022
Quote:
... and rotated at 4°C overnight with 2-5 ug of antibody (H3K27ac, Active Motif Cat #39133 ...
-
No products found
because this supplier's products are not listed.
Lu Rao, et al.,
bioRxiv - Cell Biology 2022
Quote:
... 2 µL of 10 mg/mL unlabeled tubulin (Cytoskeleton) was mixed with 2 µL o f 1 mg/mL biotin-tubulin and 2 µL of 1 mg/mL dye-labeled tubulin on ice ...
-
No products found
because this supplier's products are not listed.
Dougald M. Monroe, et al.,
bioRxiv - Biochemistry 2022
Quote:
... 4 μM lipid vesicles (Avanti Polar Lipids PS:PC:PE 3:2:5), and FV (0 ...
-
No products found
because this supplier's products are not listed.
Yaichi Kawakatsu, et al.,
bioRxiv - Plant Biology 2023
Quote:
... 50 mM 4-(4,6-dimethoxy-1,3,5-triazin-2-yl)-4-methylmorpholinium chloride (DMT-MM) (D2919, TCI, Tokyo, Japan) in 100 mM MOPS buffer (pH of 7.0 ...
-
No products found
because this supplier's products are not listed.
Samuel Thompson, et al.,
bioRxiv - Biophysics 2019
Quote:
... 6 mL of the selection culture in 2 mL centrifuge tubes was pelleted at 5000 rpm for 5 minutes at 4°C in a microcentrifuge (Eppendorf, 5242R). The supernatant was removed except for the last ∼200 µL ...
-
No products found
because this supplier's products are not listed.
Melanie Ernst, et al.,
bioRxiv - Biophysics 2023
Quote:
... 5 mg/mL in n-nonane (Alfa Aesar #A16177-AE) to form bilayers ...
-
No products found
because this supplier's products are not listed.
Anna-Leigh Brown, et al.,
bioRxiv - Neuroscience 2021
Quote:
... and 2 ug/mL doxycycline (Clontech, Cat. No. 631311). i3Neurons were then fed three times a week by half media changes ...
-
No products found
because this supplier's products are not listed.
Irina V. Chestakova, et al.,
bioRxiv - Microbiology 2023
Quote:
... A small fraction of about 4×4 mm from each tissue was transferred to a 2 mL vial with a 1/4” ceramic sphere (MP Biomedicals, Solon OH, USA) and 300 μL MagNA Pure 96 DNA Tissue Lysis Buffer (Roche Diagnostics GmbH ...
-
No products found
because this supplier's products are not listed.
Ashley N. Barlev, et al.,
bioRxiv - Immunology 2021
Quote:
... or equimolar concentrations of the Fab’2 fragments (Southern Biotech; 0.4-100 ug/mL) for 20 hours after which activation was measured using flow cytometry as described above.
-
No products found
because this supplier's products are not listed.
Paul Renauer, et al.,
bioRxiv - Cancer Biology 2023
Quote:
... 4 x 2 mm (Phenomenex). The eluents were A ...
-
No products found
because this supplier's products are not listed.
Ainsley Lockhart, et al.,
bioRxiv - Immunology 2023
Quote:
... 2 ug pEco (Addgene 12371), 72 uL 1 mg/mL polyethylenamine (Polysciences 23966) ...
-
No products found
because this supplier's products are not listed.
Karen. M. Marshall, et al.,
bioRxiv - Bioengineering 2023
Quote:
... the scaffolds seeded with C2C12 cells were moved to a 24 well plate and 1.5 mL of media was added (basal with 2% FCS ± 5 µg of BMP-2 (3.33 µg/mL BMP-2). Culture was continued for 4 days and ALP staining performed on the 5th day from cell seeding ...
-
No products found
because this supplier's products are not listed.
Yiru Long, et al.,
bioRxiv - Immunology 2024
Quote:
... 50 ng/mL of IL-2 (Z02764, GenScript), 10 ng/mL of IL-4 (HY-P70653 ...
-
No products found
because this supplier's products are not listed.
Yan Shin J. Liao, et al.,
bioRxiv - Physiology 2019
Quote:
... containing 2-4% normal goat serum (Jackson Laboratories). Tissues were incubated with primary antibodies for 2 hours at 37°C ...
-
No products found
because this supplier's products are not listed.
Manuel Peter, et al.,
bioRxiv - Neuroscience 2020
Quote:
Male 2-4 month old C57BL/6J mice (Charles River) were stereotaxically implanted with 26GA guide cannulas bilaterally (PlasticsOne ...