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No products found
because this supplier's products are not listed.
Layla Drwesh, et al.,
bioRxiv - Biochemistry 2022
Quote:
... 4 °C) and the supernatants were incubated overnight with 2 mL Ni-NTA Agarose beads (Cube Biotech). The bound proteins were washed with 20 mL wash buffer (40 mM HEPES ...
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No products found
because this supplier's products are not listed.
Martina Lerche, et al.,
bioRxiv - Cell Biology 2019
Quote:
... Easy Coat™ (SW6-EC-0.5, SW6-EC-2 EA, SW6-EC-4 EA and SW6-EC-50 EA, Matrigen) for immunoblotting samples collected from hydrogels.
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No products found
because this supplier's products are not listed.
Vignesh Jayarajan, et al.,
bioRxiv - Cell Biology 2022
Quote:
The ROCKi inhibitor Y-27632 ((R)-(+)-trans-4-(1-aminoethyl)-N-(4-pyridyl) cyclohexanecarboxamide-2) was purchased from AdooQ Bioscience (#A1101 ...
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No products found
because this supplier's products are not listed.
Yi Sak Kim, et al.,
bioRxiv - Neuroscience 2024
Quote:
BV-2 microglia cell line was cultured in DMEM with 5% FBS and 50 μg/ml gentamicin (Omega Scientific) as described previously5 ...
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2'3'-cyclic GAMP ( cGAMP ) FRET Detection / assay Kit
Cat# K081-F5,
1.0 ea, USD $1560.0
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Lisa Mohr, et al.,
bioRxiv - Molecular Biology 2020
Quote:
... 4°C for 10 min and 2′3′-cGAMP levels were quantified using the 2′3′-cGAMP ELISA Kit (Arbor Assays) according to the manufacturer’s instructions.
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No products found
because this supplier's products are not listed.
Estifanos N. Habtemichael, et al.,
bioRxiv - Physiology 2019
Quote:
... 100 μl of resuspended cells and 2-4 μg of desired plasmid DNA in 5 μL of volume were pipetted into electroporation cuvettes (2 mm gap, Bulldog Bio 12358-346) and electroporated using a NEPA21 electroporation system ...
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No products found
because this supplier's products are not listed.
Julian M. Jimenez, et al.,
bioRxiv - Bioengineering 2022
Quote:
Homogeneous fibrin gels were prepared to final fibrinogen concentrations of 2 and 4 mg/mL by combining human fibrinogen (FIB3, Enzyme Research Laboratories) and Alexa Fluor (AF ...
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No products found
because this supplier's products are not listed.
Adam McNee, et al.,
bioRxiv - Immunology 2020
Quote:
... were coated with 2 μg/ml rec 2-12C (Absolute Antibody) in PBS-T overnight at 4°C ...
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No products found
because this supplier's products are not listed.
Pengcheng Zhou, et al.,
bioRxiv - Immunology 2023
Quote:
... SEB (1 ug/mL, Toxin Technology) was used as positive control and an equimolar amount of DMSO was used as negative control ...
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No products found
because this supplier's products are not listed.
Linghao Hu, et al.,
bioRxiv - Bioengineering 2022
Quote:
... bis-2-(5-phenylacetamido-1,3,4-thiadiazol-2-yl) ethyl sulfide (BPTES, 10 μm, ASTATECH, A11656) was added to the cells in CM3 1 hour before imaging (35) ...
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No products found
because this supplier's products are not listed.
Jiechao Zhou, et al.,
bioRxiv - Neuroscience 2022
Quote:
... Purified human complement proteins (4 µg/mL, Complement Technology) were immobilized and were treated with His-tagged recombinant neuronal pentraxin proteins (4 µg/mL ...
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No products found
because this supplier's products are not listed.
Ludovic Spaeth, et al.,
bioRxiv - Neuroscience 2021
Quote:
... 4% for induction then 1-2% for the surgery) and mounted on a stereotaxic frame (Model 68526, RWD Life Science). Body temperature was monitored using a rectal probe and maintained with a heat pad ...
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No products found
because this supplier's products are not listed.
Meghal Desai, et al.,
bioRxiv - Neuroscience 2022
Quote:
... Lysates clarified by centrifugation at 10,000g for 15 minutes at 4°C were incubated with 5ul of serum containing antibodies for 2 hours at 4°C followed by the addition of pre-blocked (with 1% BSA) Protein-A beads (Repligen) for an additional 2 hours ...
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No products found
because this supplier's products are not listed.
Celeste Parra Bravo, et al.,
bioRxiv - Neuroscience 2023
Quote:
... Insoluble samples were sonicated at 16°C for 4 minutes on/off with 2-second pulses and 20% amplitude using a water bath sonicator (EpiSonic 2000, EpigenTek), centrifuged at 20,000 g for 30 minutes at 20°C and the supernatant was collected as Triton-insoluble fraction ...
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No products found
because this supplier's products are not listed.
James S. Santangelo, et al.,
bioRxiv - Evolutionary Biology 2019
Quote:
... plus 50 uL of ddH20 or (2) 80 μL of 0.2 EU / mL linamarase (LGC Standards CDX-00012238-100). A positive reaction in (1 ...
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No products found
because this supplier's products are not listed.
Venecia Valdez, et al.,
bioRxiv - Molecular Biology 2023
Quote:
... 200 μM PMSF) before flowing over 5 mL CV of Strep-Tactin Superflow resin (Neuromics: 2-1206-025). The column was then washed with 10 CV of Strep binding buffer before being eluted with 1.5 CV of elution buffer (Strep binding buffer + 3.3 mM D-desthiobiotin) ...
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No products found
because this supplier's products are not listed.
Xing Fang, et al.,
bioRxiv - Neuroscience 2022
Quote:
Cell contractile capabilities were compared using primary cerebral VSMCs (passages 2 – 4) with a collagen gel-based cell contraction assay kit (CBA-201, Cell Biolabs, San Diego, CA) following our optimized protocol 9,40,48,53,57,58 ...
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No products found
because this supplier's products are not listed.
Fang He, et al.,
bioRxiv - Genetics 2020
Quote:
... The hybridization solution is as follows: 0.6ng/ml Cy5 labeled 2’-O-Me-(CCCCGG)5 RNA probe (IDT DNA Technologies, IA), 0.02% BSA ...
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No products found
because this supplier's products are not listed.
Greg A. Timblin, et al.,
bioRxiv - Immunology 2020
Quote:
... Injection volumes were as follows: 2μL for 1mg/mL 4-OHE1 standard (Steraloids) in MeOH ...
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No products found
because this supplier's products are not listed.
Enrico Radaelli, et al.,
bioRxiv - Cell Biology 2022
Quote:
... 4-Hydroxynonenal (HNE, Alpha Diagnostic International HNE11-S ...
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No products found
because this supplier's products are not listed.
Eamim Daidrê Squizani, et al.,
bioRxiv - Microbiology 2020
Quote:
... and homogenized in 2 ml sterile PBS using a BeadBug (Benchmark Scientific). Organ homogenates were serially diluted and plated onto Sabouraud dextrose agar plates ...
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No products found
because this supplier's products are not listed.
Jinbao Liu, et al.,
bioRxiv - Plant Biology 2024
Quote:
... Then the mixture was centrifuged at 200 g for 2 min and the supernatant was transferred to a new 2 mL Tube (CELLTREAT, catalog number: 229446) and centrifuged at 1,000 g for 5 min ...
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No products found
because this supplier's products are not listed.
Carlos A. Elena-Real, et al.,
bioRxiv - Biophysics 2022
Quote:
... 150 mM NaCl) at 4°C using SpectraPor 4 MWCO 12-14 kDa dialysis tubing (Spectrum Labs). Dialyzed protein was then concentrated with 10 kDa MWCO Vivaspin centrifugal concentrators (3,500 xg ...
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No products found
because this supplier's products are not listed.
Joseph R. Francica, et al.,
bioRxiv - Immunology 2021
Quote:
... The remaining PBS was mixed with 2 mL of RNAzol BD (Molecular Research Center) and 20 μL acetic acid ...
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No products found
because this supplier's products are not listed.
Jiyoon Lee, et al.,
bioRxiv - Developmental Biology 2019
Quote:
... Cells were passaged at ∼80% confluency (generally every 4-5 days) in medium containing 10 µM Y27632 (hereafter, Y; ROCK inhibitor that inhibits apoptosis, ReproCell). WA25 cells were passaged in clusters with 0.5 mM EDTA ...
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No products found
because this supplier's products are not listed.
Christin Herrmann, et al.,
bioRxiv - Microbiology 2020
Quote:
... was added to sample 2 (DUBPAN) and 2 μl of OTUB1 (LifeSensors, Cat#: DB201) was added to sample 3 (DUBK48) ...
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No products found
because this supplier's products are not listed.
Hongmei Qiao, et al.,
bioRxiv - Genomics 2020
Quote:
... AFP4/TMAC2 (ABI FIVE BINDING PROTEIN 4, AT3G02140), DOG1 (AT5G45830 ...
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No products found
because this supplier's products are not listed.
Marco Tognetti, et al.,
bioRxiv - Cancer Biology 2021
Quote:
... 4 μg of each sample was separated using a self-packed analytical PicoFrit column (75 μm × 50 cm length) (New Objective, Woburn, MA, USA) packed with ReproSil- Saphir C18 1.5 μm (Dr ...
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No products found
because this supplier's products are not listed.
Brenden Barco, Nicole K. Clay,
bioRxiv - Plant Biology 2019
Quote:
... A single colony of Psta from a freshly streaked 3-day-old agar plate was grown in 50 mL of LB medium containing 25 μg/ml rifampicin and 50 μg/mL kanamycin (IBI Scientific, Peosta, IA).
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No products found
because this supplier's products are not listed.
Christian Heuss, et al.,
bioRxiv - Microbiology 2022
Quote:
... Sections were counterstained using 3% uranyl acetate in 70% methanol for 5 min and lead citrate (Reynold’s) for 2 min and imaged by using a JEOL JEM-1400 (JEOL) operating at 80 kV and equipped with a 4K TemCam F416 (Tietz Video and Image Processing Systems).
-
No products found
because this supplier's products are not listed.
Huiqiao Pan, et al.,
bioRxiv - Microbiology 2023
Quote:
... point style #2 needles (Hamilton Company) were used to inject 100 µL headspace samples into TRACE 1300/ISQ 7000 GC-MS equipped with a TracePLOT TG-BOND Q+ column (30 m x 0.32 mm x 10 µm ...
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No products found
because this supplier's products are not listed.
Guillaume Hummel, et al.,
bioRxiv - Plant Biology 2020
Quote:
... 250 μg/ml of sodium cefotaxime (Duchefa Biochemie, Reference: C0111) and 2 ml/L of Plant Preservative Mixture (Plant Cell Technology, PPM) in growth chambers 21°C/17°C for a photoperiod of 16h/8h (day/night).
-
No products found
because this supplier's products are not listed.
Benjamin M. Janus, et al.,
bioRxiv - Immunology 2023
Quote:
... competing antibody was diluted to a final concentration of 5 µg/mL in blocking buffer and added to 2 µg/mL GP that was biotinylated through a fused Avi-tag (Avidity, Aurora, Colorado) and incubated for one hour at room temperature ...
-
No products found
because this supplier's products are not listed.
Wei-Sheng Sun, et al.,
bioRxiv - Biochemistry 2023
Quote:
... 30 mM Imidazole and 0.02 mg/ml DNase I and broken with a Constant cell disruptor at 4 °C and 25 kPsi (Constant Systems). The cell lysate was clarified by centrifugation for 30 minutes at 30,000 x g ...
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No products found
because this supplier's products are not listed.
Kyung Bae Min, et al.,
bioRxiv - Microbiology 2020
Quote:
... and Dkstatin-2 (N-ethyl-3-[(3-fluorophenyl)methoxy]-N-[(1-methyl-1H-imidazol-5-yl)methyl]aniline) were purchased from Chembridge Corp ...
-
No products found
because this supplier's products are not listed.
Erin M. Euliano, et al.,
bioRxiv - Bioengineering 2024
Quote:
... 2 equivalents of 4-((6-Amino-2-(2-methoxyethoxy)-8-oxo-7,8-dihydro-9H-purin-9-yl)methyl)benzoic acid (Ambeed, Arlington Heights, IL), also known as 1V209 ...
-
No products found
because this supplier's products are not listed.
Raul Jobava, et al.,
bioRxiv - Molecular Biology 2020
Quote:
... harringtonine (2 μg/mL) (LKT Laboratories, H0169), sephin 1 (Apexbio,A8708 ...
-
No products found
because this supplier's products are not listed.
Julia Ledderose, et al.,
bioRxiv - Neuroscience 2021
Quote:
... Time-pregnant female mice were injected intraperitoneally with 50 mg/kg BrdU (5-bromo-2’-deoxyuridine, BrdU; Accurate Chemical & Scientific Corporation) at E11.5 ...
-
No products found
because this supplier's products are not listed.
Mengmeng Jin, et al.,
bioRxiv - Neuroscience 2024
Quote:
... cells were passaged every 4∼5 days with Accutase (Innovative Cell Technologies) onto Matrigel (BD Biosciences)-coated culture plates at a ratio of 1:4∼1:8 supplemented with ROCK inhibitor Y-27632 (10 mM ...
-
No products found
because this supplier's products are not listed.
David Bradley, et al.,
bioRxiv - Evolutionary Biology 2023
Quote:
... anti-CENP-C (PD030, MBL International, 1 ug/ml) and anti-CENP-A (3-19 ...
-
No products found
because this supplier's products are not listed.
Changdong He, Chun Ying Wu, Wan Li, Ke Xu,
bioRxiv - Biophysics 2023
Quote:
... Methoxy-PEG silane (MW 5000, PG1-SL-5k, Nanocs) was dissolved in dry DMSO at 30 mg mL−1 at 60°C ...
-
No products found
because this supplier's products are not listed.
Valeria Garcia-Flores, et al.,
bioRxiv - Immunology 2022
Quote:
... the slides were incubated with DAPI (4′,6-diamidino-2-phenylindole) as a nuclear counterstain and mounted using AquaSlip™ Aqueous Permanent Mounting Medium (American MasterTech). Fluorescence image acquisition was performed using the Vectra Polaris Multispectral Imaging System at 20x magnification ...
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No products found
because this supplier's products are not listed.
James D. Dahlvang, et al.,
bioRxiv - Immunology 2022
Quote:
... PBMCs were counted and resuspended at 2×108 cells/mL in 10mL of PBS + 2% FBS (PEAK Serum, PS-FB1) + 1 mM EDTA (SC Buffer) ...
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No products found
because this supplier's products are not listed.
Carolina Rosselot, et al.,
bioRxiv - Cell Biology 2021
Quote:
... Exendin-4 plasma levels were measured using the exendin-4 EIA kit (Phoenix pharmaceuticals, Burlingame, CA). Harmine was measured in plasma by liquid chromatography–mass spectrometry analysis by WuXi AppTec (Cranbury ...
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No products found
because this supplier's products are not listed.
Vanessa Meier-Stephenson, et al.,
bioRxiv - Biophysics 2021
Quote:
... An HBsAg dilution series (500 ng/mL - 4 ng/mL) was prepared for use as standards (Fitzgerald, Cat #C-CP2019R). To each corresponding well ...
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No products found
because this supplier's products are not listed.
Arun Upadhyay, et al.,
bioRxiv - Neuroscience 2023
Quote:
... Stock solutions of recombinant Aβ peptides were prepared by solubilizing lyophilized monomers (Aβ38, rPeptide A-1078-2; Aβ40:rPeptide A-1153-2; and Aβ42: rPeptide A-1163-2) in 1% NH4OH at 200 µM concentration as recommended by manufacturer ...
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No products found
because this supplier's products are not listed.
Ian W. McCahill, et al.,
bioRxiv - Plant Biology 2024
Quote:
... 2 media (Caisson Labs). Aqueous solutions of paclobutrazol and GA3 were freshly prepared and diluted to 0.1 µM and 10 µM respectively in hydroponic media ...
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No products found
because this supplier's products are not listed.
Breanne A. Cameron, et al.,
bioRxiv - Physiology 2022
Quote:
... and Fluo-5F emission was collected with a 525 / 50 nm bandpass filter (FF03-525 / 50, Semrock) and di-4-ANBDQPQ emission with a 700 nm long-pass filter (HQ700lp; Chroma Technology). A schematic of the imaging setup is provided in Fig ...
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Magnetofection
diificult to transfect cells
Cat# KM30400,
SilenceMag 200µL + PolyMag 100µL+ CombiMag 100µL + PolyMag Neo 100µL, USD $338.00/KIT
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Zeyang Shen, et al.,
bioRxiv - Genomics 2021
Quote:
... 2.5×105cells in 1 ml were transduced with 2 ml of ER-HoxB8 retrovirus (in DMEM with 30% FBS) containing 0.5 μl/ml lentiblast A (OZ Biosciences), 2.5 μl/ml lentiblast B (OZ Biosciences ...
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No products found
because this supplier's products are not listed.
Giulia Ferrari, et al.,
bioRxiv - Cell Biology 2021
Quote:
... FACS data analysis was done using FCS Express 4 (De Novo Software). A similar procedure was followed for FACS cell purification ...