-
No products found
because this supplier's products are not listed.
Clara R. Stelman, et al.,
bioRxiv - Developmental Biology 2020
Quote:
... 5-Bromo-4-chloro-3-indolyl phosphate (BCIP, [Roche]) and nitro blue tetrazolium chloride (NBT ...
-
No products found
because this supplier's products are not listed.
Adva Mechaly, et al.,
bioRxiv - Biochemistry 2021
Quote:
... 5-Bromo-4-Chloro-3-Indolyl-phosphate (BCIP, Sigma B8503) was used as the chromogenic substrate.
-
No products found
because this supplier's products are not listed.
Katherine M. Oliver, et al.,
bioRxiv - Neuroscience 2020
Quote:
... and 0.175 g/ml 5-bromo-4-chloro-3-indolyl-phosphate (BCIP) (Invitrogen). Alkaline phosphatase staining reaction was proceeded o/n at RT ...
-
No products found
because this supplier's products are not listed.
Gabriel A. Yette, Scott Stewart, Kryn Stankunas,
bioRxiv - Developmental Biology 2021
Quote:
... and 175 μg/ml 5-bromo-4-chloro-3-indolyl-phosphate (BCIP) (Promega). Reactions were stopped with three PBS rinses ...
-
No products found
because this supplier's products are not listed.
Valeria Scagliotti, et al.,
bioRxiv - Developmental Biology 2022
Quote:
... and 5-Bromo-4-chloro-3-indolyl phosphate disodium salt (BCIP, 11383221001, Merck-SIGMA). Sections were mounted using DPX (6522 ...
-
No products found
because this supplier's products are not listed.
Samir Jana, et al.,
bioRxiv - Cancer Biology 2023
Quote:
... 5-bromo-4-chloro-3-indoxylphosphate/nitroblue tetrazolium chloride (BCIP/NBT) substrate (Abcam cat # ab7468).
-
No products found
because this supplier's products are not listed.
Justin E. Silpe, et al.,
bioRxiv - Microbiology 2021
Quote:
... 40 μg mL−1 5-Bromo-4-Chloro-3-Indolyl-beta-D-Galactosidase (X-gal, Takara Bio), and 500 μM isopropyl beta-D-1-thiogalactopyranoside (IPTG ...
-
No products found
because this supplier's products are not listed.
Kate Harris, et al.,
bioRxiv - Neuroscience 2024
Quote:
... 4-(2-Butyl-6,7-dichloro-2-cyclopentyl-indan-1-on-5-yl) oxobutyric acid (DCPIB; Tocris), superoxide dismutase (SOD ...
-
No products found
because this supplier's products are not listed.
Anne D. Villela, et al.,
bioRxiv - Microbiology 2020
Quote:
... Membranes were washed three times with 1X PBS for 5 min and developed with nitro blue tetrazolium/5-bromo-4-chloro-3-indolyl phosphate in alkaline phosphatase buffer (Bio-Rad).
-
No products found
because this supplier's products are not listed.
Liang Wang, et al.,
bioRxiv - Cell Biology 2023
Quote:
... #4: 5’-CCGGTTTAGCTGAAGATTCAA-3’ (SI00443779, Qiagen), GTPBP10 siRNA 5’-TTGCGTGTTGTTCAGAAAGTA-3’ (SI04308647 ...
-
No products found
because this supplier's products are not listed.
Bradley R Corr, et al.,
bioRxiv - Cancer Biology 2023
Quote:
... 5′-bromo-2′-deoxyuridine (BrdU) (Cat. #550891; BD Biosciences; RRID:AB_2868906) was then added directly to the well culture media (final concentration 10 µM) ...
-
No products found
because this supplier's products are not listed.
Sara F. Costa, et al.,
bioRxiv - Microbiology 2023
Quote:
... with 100 μg/mL 5-bromo-4-chloro-3-indolyl-β-D-galactopyranoside (X-Gal, VWR), or with 0.1 μM CdCl2 (Sigma-Aldrich) ...
-
No products found
because this supplier's products are not listed.
Alexia Polissidis, et al.,
bioRxiv - Neuroscience 2021
Quote:
For GBA quantification (n=5 for 4 WPI, n=3 for 8 WPI), the surface function of the Imaris software was used to select only GFP-positive cells by appropriately adjusting the number of voxels as well as the intensity of the GFP channel in the threshold tab ...
-
No products found
because this supplier's products are not listed.
Sean R. Cuddy, et al.,
bioRxiv - Microbiology 2020
Quote:
... ZD 7288 and 8-bromo-cyclic AMP (Cayman Chemicals); Nerve Growth Factor 2.5S (Alomone Labs) ...
-
No products found
because this supplier's products are not listed.
Konstadinos Moissoglu, et al.,
bioRxiv - Cell Biology 2020
Quote:
... sgRNAs targeting RABIF (sg #2: 5’-GAGCGAGTTAGTGTCAGCCGAGG-3’ and sg #4 5’-AGCGAGTTAGTGTCAGCCGAGGG-3’) were cloned in lentiCRISPRv2 (Addgene #52961). MDA-MB-231 cells were infected with the corresponding lentiviruses and selected with 1μg/ml puromycin (Thermo Fisher Scientific).
-
No products found
because this supplier's products are not listed.
Virginia Lioy, et al.,
bioRxiv - Microbiology 2021
Quote:
... The digested DNA (4 ml in total) was split into 4 aliquots and diluted in 8 ml ligation buffer (1X ligation buffer NEB 3 (without ATP), 1 mM ATP ...
-
No products found
because this supplier's products are not listed.
Meghan Robinson, et al.,
bioRxiv - Bioengineering 2021
Quote:
... 0.5 mM RA and 1 mM 8-bromo-cAMP (Peprotech, 2354843) were added ...
-
No products found
because this supplier's products are not listed.
José M. González Dalmasy, et al.,
bioRxiv - Pharmacology and Toxicology 2023
Quote:
... Dichloro-α-methylbenzylamine (DCMB, LY78335) was from Santa Cruz Biotechnology (Dallas ...
-
No products found
because this supplier's products are not listed.
Andrew L. Eagle, et al.,
bioRxiv - Neuroscience 2024
Quote:
... Male C57Bl/6J mice (3–5/cage, 7–8-week old upon arrival from Jackson Labs) were allowed at least 5 d to acclimate to the facility prior to any experimental procedures ...
-
No products found
because this supplier's products are not listed.
Yun Deng, et al.,
bioRxiv - Developmental Biology 2022
Quote:
... with 5-bromo-4-chloro-3-indolyl-phosphate nitro blue tetrazolium staining (Vector Laboratories, Burlingame, CA, USA). 10 ∼ 30 embryos were used for each probe ...
-
No products found
because this supplier's products are not listed.
Verena Schmied, et al.,
bioRxiv - Neuroscience 2024
Quote:
... 4-5 3D-organoids were placed into one well of an 8-well chamber (IBIDI, #80826) and covered with 3% (w/v ...
-
No products found
because this supplier's products are not listed.
Divyesh Joshi, et al.,
bioRxiv - Cell Biology 2024
Quote:
... pSmad1/5/8 (Cell Signaling Technology), GAPDH (Sigma Chemical Co. ...
-
No products found
because this supplier's products are not listed.
Masahito Yamagata, Wenjun Yan, Joshua R. Sanes,
bioRxiv - Neuroscience 2020
Quote:
... In situ hybridization using nitro-blue tetrazolium and 5-bromo-4-chloro-3′-indolyphosphate and double color in situ hybridization using TSA Plus (PerkinElmer) were performed as previously described (Yamagata et al.,1999 ...
-
No products found
because this supplier's products are not listed.
Dougald M. Monroe, et al.,
bioRxiv - Biochemistry 2022
Quote:
... 4 μM lipid vesicles (Avanti Polar Lipids PS:PC:PE 3:2:5), and FV (0 ...
-
No products found
because this supplier's products are not listed.
Fang Xu, et al.,
bioRxiv - Neuroscience 2020
Quote:
... 8% (w/v) paraformaldehyde (PFA; 4% final concentration; 157-8, Electron Microscopy Sciences), distilled water ...
-
No products found
because this supplier's products are not listed.
Briana Zellner, et al.,
bioRxiv - Microbiology 2021
Quote:
... Groups of 4-8 female C3H/HeN mice (6-8 weeks old; Charles River Laboratories) were anesthetized with a ketamine-xylazine sedative and intranasally (i.n. ...
-
No products found
because this supplier's products are not listed.
Julianna L. Sun, et al.,
bioRxiv - Microbiology 2022
Quote:
... Membranes were developed using 5-bromo-4-chloro-3-indolyl phosphate (BCIP)/nitro blue tetrazolium (NBT) (Sigma Aldrich, 11697471001. Images were captured using a Nikon Z1000 microscope.
-
No products found
because this supplier's products are not listed.
Ole A.W. Haabeth, et al.,
bioRxiv - Immunology 2021
Quote:
... After washing wells were incubated with a 5-bromo-4-chloro-3⍰-indolyl phosphate (BCIP)/nitro blue tetrazolium (NBT) substrate (R&D systems, mouse IFNγ Kit Cat # EL485). Plates were scanned and analyzed using ImmunoSpot Microanalyzer.
-
No products found
because this supplier's products are not listed.
Alberto Herrera, et al.,
bioRxiv - Immunology 2024
Quote:
... #3 and #4 (Biolegend) were spiked into individual samples ...
-
No products found
because this supplier's products are not listed.
Timothy J. Mottram, et al.,
bioRxiv - Microbiology 2023
Quote:
... 4 μl of the 3’-5’-adapter-ligated RNA was mixed with barcoded RT primers (Illumina) and cDNA synthesis was performed using SuperScript™ II Reverse Transcriptase (Invitrogen ...
-
No products found
because this supplier's products are not listed.
N. Sumru Bayin, et al.,
bioRxiv - Developmental Biology 2020
Quote:
... Image stacks were obtained every 3-4 minutes for 6-8 hours using a LSM880 (Leica) with an environmental chamber (37°C with 5% CO2) ...
-
No products found
because this supplier's products are not listed.
Peng Liu, Ian P. Lapcinski, Karen H. Ashe,
bioRxiv - Biochemistry 2024
Quote:
... that recognizes amino acids 3-8 of Aβ and the Protein G Sepharose 4 Fast Flow resin (GE healthcare) (Tables 2 and 3 ...
-
No products found
because this supplier's products are not listed.
Elvin Wagenblast, et al.,
bioRxiv - Cancer Biology 2020
Quote:
... Between 1×10^3 – 5×10^4 cells were re-suspended in 20μl Buffer P3 (Lonza) per reaction and quickly added to the Eppendorf tube containing the CRISPR/Cas9 gRNA RNP complex ...
-
No products found
because this supplier's products are not listed.
Jinhyung Lee, et al.,
bioRxiv - Bioengineering 2024
Quote:
Female 6- to 8-week-old NU/J mice were inoculated subcutaneously with 5 × 106 SKOV-3 cells supplemented with Matrigel (Corning, #354234), and allowed to grow for 4 weeks until the tumor size was 200 mm3 ...
-
No products found
because this supplier's products are not listed.
Aleksandra Boikova, et al.,
bioRxiv - Molecular Biology 2023
Quote:
... Cardiomyocyte specification was completed by 3 days of culture in RPMI B27-media with 5 μM IWP-4 (STEMCELL Technologies), followed by another 7 days of culture with RPMI B27+ (RPMI 1640 GlutaMAX + 2% B27 supplement with insulin (ThermoFisher) ...
-
No products found
because this supplier's products are not listed.
Walden Li, Ryan M. Wyllie, Paul A. Jensen,
bioRxiv - Microbiology 2020
Quote:
... Strains carrying the pLacZ plasmid appear blue when plated on 0.008% X-gal (5-bromo-4-chloro-3-indolyl β-D-galactopyranoside) (Zymo Research, CA, USA). X-gal concentrations above 0.008% appear to inhibit growth of S ...
-
No products found
because this supplier's products are not listed.
Sophie Dürauer, et al.,
bioRxiv - Biochemistry 2024
Quote:
... and cell numbers were recorded every 8 h for 3 days using Cytation 5 (BioTek) equipped with a 4x objective and the Gen5 software (ver ...
-
No products found
because this supplier's products are not listed.
Han Zhu, et al.,
bioRxiv - Cell Biology 2022
Quote:
Samples were incubated on a rotator for 5 min at 4°C and then centrifuged at 500g for 5 min (Eppendorf, 5920R; 4°C, ramp speed of 3/3). Supernatant was removed and pellet was resuspended in sort buffer [1mM EDTA (Invitrogen ...
-
No products found
because this supplier's products are not listed.
Matthew J. Burke, et al.,
bioRxiv - Molecular Biology 2022
Quote:
... a J2-4 reverse primer 5’-(GGATAGATCTCTGAGGAGACGGTGACCAGGAG-3’) and HERC II polymerase (Agilent), following the manufacturer’s recommended reaction conditions ...
-
No products found
because this supplier's products are not listed.
Buse Baran, et al.,
bioRxiv - Plant Biology 2024
Quote:
... Guide RNA sequences “5-CGUGUCACGACGGAGUGGAU-3” and “5-AUGUUGUAUGUGCCUAUACG-3” were synthesized by GenScript by adding the Cas12a scaffold sequence (5-UAAUUUCUACUCUUGUAGAU-3 ...
-
No products found
because this supplier's products are not listed.
Antonio Marino, et al.,
bioRxiv - Systems Biology 2023
Quote:
... 4 3 2.0 mm SecurityGuard (Phenomenex) cartridge as a guard column ...
-
No products found
because this supplier's products are not listed.
Lamuk Zaveri, Jyotsna Dhawan,
bioRxiv - Cell Biology 2021
Quote:
... Klf4 or Oct-3/4 or Klf4 + Oct-3/4 or Klf4 + OctER using PEI (Polysciences, USA). Self-ligated empty pGEM-T vector (Promega ...
-
No products found
because this supplier's products are not listed.
Emily N. P. Prowse, et al.,
bioRxiv - Cell Biology 2024
Quote:
... We loaded 2 mL of the homogenate mixture on top of a 3.92 ml 62% sucrose in MAB cushion in an Ultra-Clear 5/8” X 4” Beckman Coulter tube (344061, Beckman Coulter). We added the remaining sucrose in MAB solutions ...
-
No products found
because this supplier's products are not listed.
Anna L. Vlasits, et al.,
bioRxiv - Neuroscience 2024
Quote:
... Boroscillate pipettes (Sutter Instruments, 3-5 MΩ) were used for all recordings and whole-cell electrophysiology recordings were performed using a Multiclamp 700B amplifier (Molecular devices) ...
-
No products found
because this supplier's products are not listed.
J. Christopher Rounds, et al.,
bioRxiv - Neuroscience 2021
Quote:
... sonicated for 3×5 minutes in a 4°C Bioruptor ultrasonicator (UCD-200, Diagenode), vortexed ...
-
No products found
because this supplier's products are not listed.
Tai L. Ng, et al.,
bioRxiv - Systems Biology 2021
Quote:
... 2’,3’-cyclic GMP-AMP (cGAMP) (Invivogen #tlrl-nacga23-5) at a concentration of 1 mg/mL (final concentration in the well 100 ug/mL ...
-
No products found
because this supplier's products are not listed.
Mariano Nicolás Di Guilmi, et al.,
bioRxiv - Neuroscience 2021
Quote:
... with a high-pass Bessel (8-pole) filter with a −3 dB cutoff of 5 Hz using Clampfit 10.6 (Molecular Devices). All data was analyzed using NeuroMatic in Igor Pro software (WaveMetrics ...
-
No products found
because this supplier's products are not listed.
Nioosha Nekooie-Marnany, et al.,
bioRxiv - Developmental Biology 2022
Quote:
... Cy-3 or Cy-5 (Jackson Immunoresearch Laboratories), and processed for DAPI or Hoechst staining to visualize cells’ nuclei before mounting in ImmuMount medium (Shandon) ...
-
No products found
because this supplier's products are not listed.
Valentina Salvi, et al.,
bioRxiv - Immunology 2021
Quote:
... and PE-conjugated anti-IL-4 (clone 7A3-3, Miltenyi Biotec) following the manufacturer’s recommendations ...
-
No products found
because this supplier's products are not listed.
E. Koeksoy, et al.,
bioRxiv - Microbiology 2021
Quote:
... (2016) for 5 to 8 days prior to analyses with an Olympus BX60 epifluorescence microscope (Olympus, Japan) and a Qicam 1394 (Qimaging ...