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No products found
because this supplier's products are not listed.
Thomas O’Loughlin, et al.,
bioRxiv - Cell Biology 2019
Quote:
... 2’,3’-cGAMP (Invivogen) at 10 μg/mL ...
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2'3'-cyclic GAMP ( cGAMP ) FRET Detection / assay Kit
Cat# K081-F1,
1.0 ea, USD $390.0
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Prashant P. Damke, et al.,
bioRxiv - Microbiology 2022
Quote:
... and cGAMP levels were quantified using the DetectX® 2’,3’-cGAMP STING-Based FRET assay (Arbor Assays). For each experimental condition ...
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No products found
because this supplier's products are not listed.
Ahmed M. Fahmy, et al.,
bioRxiv - Immunology 2023
Quote:
... 2’’3’’-cGAMP ELISA was done using the 2’’3’’-cGAMP ELISA Kit (Cayman Chemical Co.) according to the manufacturer’s instructions.
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No products found
because this supplier's products are not listed.
Shurong Zhou, et al.,
bioRxiv - Bioengineering 2023
Quote:
... The concentrations of 2’3’-cGAMP was measured using 2’3’-cyclic cGAMP ELISA kit (Invitrogen) following the manufacturer’s instruction.
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No products found
because this supplier's products are not listed.
Amy E. Spens, et al.,
bioRxiv - Developmental Biology 2022
Quote:
Myoblasts were transfected with either (final concentrations) 10 µM 2’,3’-cGAMP (Sigma Aldrich), 2 µg/mL poly(I:C ...
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No products found
because this supplier's products are not listed.
Dario Zimmerli, et al.,
bioRxiv - Cancer Biology 2021
Quote:
... STING (1:1000, Cell Signaling, #13647), cMYC (1:200 ...
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No products found
because this supplier's products are not listed.
David A. Lomas, et al.,
bioRxiv - Pharmacology and Toxicology 2020
Quote:
... TR-FRET detection was performed on an Envision plate reader (Perkin Elmer).
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No products found
because this supplier's products are not listed.
Hina Khan, et al.,
bioRxiv - Microbiology 2023
Quote:
... cAMP levels in the cells were determined in a plate reader by using fluorescence-based cAMP detection kit (Abcam) according to the manufacturer’s recommendations and normalized to the total protein present in the samples as determined by a BCA protein estimation kit (Pierce) ...
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No products found
because this supplier's products are not listed.
Jelle Verhoeven, et al.,
bioRxiv - Cancer Biology 2023
Quote:
... anti-STING (Proteintech #19851-1-AP), anti-LMAN1 (Invitrogen #MA5-25345) ...
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No products found
because this supplier's products are not listed.
Li Wang, et al.,
bioRxiv - Cell Biology 2022
Quote:
... The FRET-based VE-cad-TS (Addgene Plasmid# 45848) was used to measure tension on VE-cadherin bonds ...
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No products found
because this supplier's products are not listed.
Matteo Rossi, et al.,
bioRxiv - Cancer Biology 2021
Quote:
... based on routine testing with MycoAlert Mycoplasma Detection Kit (Lonza).
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No products found
because this supplier's products are not listed.
Haoyang Li, et al.,
bioRxiv - Immunology 2022
Quote:
2′3′-cGAMP (0.2 μM) and dsDNA (2 μg/mL) was transfected into cells using FuGENE HD Transfection Reagent (Promega), whereas the transfection reagent without dsDNA or 2′3′-cGAMP was added as the control ...
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No products found
because this supplier's products are not listed.
Dane H. Tow, et al.,
bioRxiv - Cancer Biology 2023
Quote:
... Detection was performed using the polymer-based Envision FLEX+ detection system (Agilent).
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No products found
because this supplier's products are not listed.
Philip Pickford, et al.,
bioRxiv - Pharmacology and Toxicology 2019
Quote:
... with TR-FRET measured in a Flexstation 3 plate reader (Molecular Devices) using the following settings ...
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No products found
because this supplier's products are not listed.
Ivan Alić, et al.,
bioRxiv - Neuroscience 2020
Quote:
... The FRET (BACE2 R&D Systems, not based on amyloid sequence) control peptide (10 µM ...
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No products found
because this supplier's products are not listed.
Evan P.S. Pratt, et al.,
bioRxiv - Pharmacology and Toxicology 2019
Quote:
... Islets were plated in 24-well plates (30-40 islets/well) for FRET-based imaging experiments or ultra-low attachment 60 mm culture dishes (Corning Life Sciences, Corning, NY) for single islet insulin secretion assay ...
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No products found
because this supplier's products are not listed.
Wei Wei, et al.,
bioRxiv - Cell Biology 2021
Quote:
... apoptotic cells in pancreas frozen sections were detected by the TUNEL (TdT mediated dUTP nick end labeling) assay using a fluorescein-based detection kit (in situ death detection kit, Roche, Indianapolis, IN) according to manufacturer’s instructions ...
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No products found
because this supplier's products are not listed.
Zhi Huang, et al.,
bioRxiv - Cancer Biology 2021
Quote:
... 3’-diaminobezine detection kit (Vector Laboratories) as previously described 23 ...
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No products found
because this supplier's products are not listed.
Zackie Aktary, et al.,
bioRxiv - Molecular Biology 2024
Quote:
FRET melting competition (FRET-MC) experiments were performed in 96-well plates using a CFX96 qPCR Real-Time PCR (Biorad). The tested sequences (or competitors ...
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No products found
because this supplier's products are not listed.
Kumari Aditi, et al.,
bioRxiv - Neuroscience 2021
Quote:
... using SYBR-Green based detection system with QuantiNova SYBR green PCR Kit (Qiagen, 208052). Relative expression of mRNA was calculated through ΔΔCt method using the ribosomal protein RP49 RNA expression as internal control for each sample ...
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No products found
because this supplier's products are not listed.
Jeffrey Quigley, Kim Lewis,
bioRxiv - Microbiology 2021
Quote:
... FRET-based fluorescence of single cells were collected on BD FACS Aria II flow cytometer (BD Biosciences, San Jose, CA, USA) with a 70-μm nozzle ...
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No products found
because this supplier's products are not listed.
Mayoko Tsuji, et al.,
bioRxiv - Immunology 2023
Quote:
... the mice were intranasally sensitized using 1 μg of 2′3′-cyclic GMP-AMP (cGAMP, 531889; Merck Darmstadt, Germany) and 1 μg of (HDM ...
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No products found
because this supplier's products are not listed.
Umed Boltaev, et al.,
bioRxiv - Neuroscience 2024
Quote:
... The time resolved fluorescence of the donor and FRET based emission of the acceptor were recorded using the Synergy Neo2 plate reader (BioTek) using an excitation wavelength of 330/80 nm and emission wavelengths at 620/10 nm and 665/8 nm via Biotek IP1 dual filter cubes (Biotek ...
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No products found
because this supplier's products are not listed.
Tapas Pradhan, et al.,
bioRxiv - Cancer Biology 2020
Quote:
... Quantitative real-time PCR was performed using SYBR-Green based fluorescence detection kit (TAKARA, Japan) and HT9700 detection system (AB ...
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No products found
because this supplier's products are not listed.
Jessica Tittelmeier, et al.,
bioRxiv - Molecular Biology 2022
Quote:
... were used for subsequent ECF-based detection (GE Healthcare).
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No products found
because this supplier's products are not listed.
Jacqueline M Leung, et al.,
bioRxiv - Cell Biology 2019
Quote:
... and detected using components based on the Phototope-Star detection kit (New England BioLabs, Cat# N7020). All T ...
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No products found
because this supplier's products are not listed.
J. S. Paradis, et al.,
bioRxiv - Biophysics 2022
Quote:
... ERK phosphorylation was evaluated using a Homogenous Time-Resolved FRET (HTRF)-based Phospho-ERK (Thr202/Tyr204) cellular kit (Cisbio). Cells were lysed for 30 minutes with the lysis buffer provided and incubated for 2 hours with pERK1/2-specific antibodies conjugated with Eu3+-cryptate donor and d2 acceptor at recommended dilutions ...
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No products found
because this supplier's products are not listed.
Erica Barini, et al.,
bioRxiv - Neuroscience 2021
Quote:
... and DAB-based detection (Leica, Germany). For frozen ...
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No products found
because this supplier's products are not listed.
Sereina O. Sutter, et al.,
bioRxiv - Microbiology 2024
Quote:
... anti-STING (Santa Cruz Biotechnology E-20 ...
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No products found
because this supplier's products are not listed.
Sofia Karkampouna, et al.,
bioRxiv - Cancer Biology 2020
Quote:
... and subsequently diluted 1:125 in culture medium and added to quadrant 1, 2 and 3 (respectively for 10mM, 1mM and 0.1mM stock plates) of deepwell 384-well plates (#781271, Greiner Bio-One). DMSO-stock solutions of control molecules (100% DMSO as negative control and as positive controls we included 1000-times concentrated docetaxel [30uM] ...
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No products found
because this supplier's products are not listed.
Timothy Morris, et al.,
bioRxiv - Cell Biology 2021
Quote:
... Plasmids for alpha-actinin-4 FRET-based tension sensors were synthesized and sequenced by Genscript. Alpha-actinin-4 sstFRET408 was constructed by inserting the sstFRET module between 408aa and 409aa of human alpha-actinin-4 (XP_016882820.1) ...
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No products found
because this supplier's products are not listed.
Mamoru Ishii, et al.,
bioRxiv - Developmental Biology 2020
Quote:
... and emission filters: BA460-500 for CFP and BA520-560 for FRET detection (Olympus).
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No products found
because this supplier's products are not listed.
Aykut Shen, et al.,
bioRxiv - Molecular Biology 2023
Quote:
... and 2) Detection from Illumina RNA-seq data through SL leader sequence trimming(43) ...
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No products found
because this supplier's products are not listed.
Lihua He, et al.,
bioRxiv - Cell Biology 2020
Quote:
Planar lipid bilayers were prepared by painting a 0.2 mm hole drilled in a Teflon cup with a phospholipid solution in n-decane containing a 3:1 mixture of 1-palmitoyl-2-oleoyl-sn-glycerol-3-phosphoethanolamine and 1-palmitoyl-2-oleoylsn-glycerol-3-phosphoserine (Avanti Polar Lipids). The lipid bilayer separated 1.0 ml of solution (cis side ...
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No products found
because this supplier's products are not listed.
Mingeum Jeong, et al.,
bioRxiv - Immunology 2022
Quote:
... Annexin V Apoptosis Detection Kit (Biolegend) was used ...
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No products found
because this supplier's products are not listed.
Matteo Gentili, et al.,
bioRxiv - Immunology 2022
Quote:
... For STING-HA DNA was extracted with Quick-DNA FFPE kit (Zymo). Sequencing was performed as described in Fulco et al 201615 ...
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No products found
because this supplier's products are not listed.
Rahul Sharma, et al.,
bioRxiv - Immunology 2024
Quote:
... STING inhibitor H151 (5 µM, Tocris Bioscience), or Rapamycin (2 µM ...
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No products found
because this supplier's products are not listed.
Saswata S Sarkar, et al.,
bioRxiv - Biochemistry 2019
Quote:
... A plate-based fluorescence instrument (Tecan model – Infinite M200 PRO) was used to collect fluorescence data ...
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No products found
because this supplier's products are not listed.
Germanna Righetto, et al.,
bioRxiv - Plant Biology 2019
Quote:
... FRET signal was acquired using a ClarioStar fluorescence plate reader (BMG Labtech) (excitation/emission wavelengths of 330 and 620/650 nm ...
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No products found
because this supplier's products are not listed.
E. Priglinger, et al.,
bioRxiv - Molecular Biology 2020
Quote:
The bead-based multiplexed FACS-based MACSPlex Exosome Kit (Miltenyi Biotec, Germany) is an assay for the analysis of surface markers present on EVs ...
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No products found
because this supplier's products are not listed.
Pablo D. Jimenez Castro, et al.,
bioRxiv - Zoology 2019
Quote:
... Agar-based assay plates were prepared by adding 70 μl of 2% Agar (Bacto Agar, VWR, Becton Dickinson Sparks, MD) and 70 μl of thiabendazole solution to each well ...
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No products found
because this supplier's products are not listed.
KR Bowles, et al.,
bioRxiv - Neuroscience 2023
Quote:
... Each plate was then split 1:2 using ReLeSR (StemCell Technologies), with half of the cells used for DNA extraction for Sanger sequencing ...
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No products found
because this supplier's products are not listed.
Davide De Forni, et al.,
bioRxiv - Microbiology 2021
Quote:
... and an ELISA assay (SARS-CoV-2 Nucleocapsid Detection ELISA Kit, Sino Biological) was performed to measure produced virus through the quantification of the viral NP nucleoprotein (a measure of viral replication capacity).
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No products found
because this supplier's products are not listed.
Amelia E. Hinman, et al.,
bioRxiv - Microbiology 2021
Quote:
... Sting-/- (C57BL/6J-Stinggt/J, Jackson Laboratories strain # 017537), TLR4-/- (Jackson Laboratories strain #007227 ...
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No products found
because this supplier's products are not listed.
Amalia Sintou, et al.,
bioRxiv - Immunology 2019
Quote:
... Apoptosis assays were performed using Nucleocounter NC-3000 and a Flexicyte apoptosis/necrosis detection kit based on staining with caspase 3 substrate NucView 488 and RedDot 2 (all Biotium, Cambridge Bioscience, UK). Apoptotic cells are defined as NucView488 positive and RedDot2 negative ...
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No products found
because this supplier's products are not listed.
Yahaya A. Yabo, et al.,
bioRxiv - Cancer Biology 2023
Quote:
... an Opal 3-Plex Manual Detection Kit (Akoya Biosciences) was used following the manufacturer’s guidelines ...
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No products found
because this supplier's products are not listed.
Kelsie J. Anson, Giulia A. Corbet, Amy E. Palmer,
bioRxiv - Cell Biology 2020
Quote:
... we performed an in-vitro plate-based fluorescence assay using recombinant human MKP-3 (Enzo Life Sciences) supplied in 20 mM Tris/HCl ...
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No products found
because this supplier's products are not listed.
Nina Le Bert, et al.,
bioRxiv - Immunology 2022
Quote:
... the plates were developed with human biotinylated IFN-γ detection antibody (7-B6-1, Mabtech; 1:2000), followed by incubation with Streptavidin-AP (Mabtech ...
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No products found
because this supplier's products are not listed.
Bruno Raposo, et al.,
bioRxiv - Immunology 2022
Quote:
... 0·1% SDS) and detection with HRP conjugated Fab’2 goat anti-human IgG(γ) (Jackson Immunoresearch) and TMB substrate (Biolegend) ...
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No products found
because this supplier's products are not listed.
Julia K. Rotow, et al.,
bioRxiv - Cancer Biology 2019
Quote:
... and maintained in culture for a total of approximately 2-3 months in DMEM supplemented with 1 ng/mL IL-3 (Peprotech). Mycoplasma testing was not performed ...