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Citations for Thermo Fisher :
1 - 50 of 10000+ citations for 2' 3' cGAMP STING based FRET Detection Kit 1 Plate since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Bioengineering 2023Quote: ... The concentrations of 2’3’-cGAMP was measured using 2’3’-cyclic cGAMP ELISA kit (Invitrogen) following the manufacturer’s instruction.
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bioRxiv - Microbiology 2020Quote: ... Poly (I:C) and 2’-3’ cGAMP were delivered into cells using Lipofectamine 2000 (Thermo Fisher, USA) as described previously.
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bioRxiv - Molecular Biology 2022Quote: ... A luminol-based enhanced chemiluminescence (ECL) detection kit (Thermo Fisher Scientific) was used to visualize bound antibodies ...
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bioRxiv - Pharmacology and Toxicology 2019Quote: ... plates were treated with 2 μM of the CellEvent™Caspase-3/7 green detection reagent (Life Technologies, UK) for 60 minutes at 37 °C in the dark ...
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bioRxiv - Cell Biology 2021Quote: FRET experiments were conducted in black plastic 96-well plates (Nunc) using a CLARIOstar plate reader (BMG LABTECH) ...
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bioRxiv - Biochemistry 2020Quote: ... and horseradish peroxidase-based chemiluminescence detection (SuperSignal® West Pico kit, Thermo Scientific).
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bioRxiv - Cancer Biology 2023Quote: ... the CellEvent Caspase-3/7 Green Detection kit (Invitrogen) was used with propidium iodide as a viability marker ...
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bioRxiv - Cancer Biology 2023Quote: While the FRET plates were being read in the Appliskan (Thermo Scientific), the imaging assay was prepared ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... The plate was centrifuged at 2,000 × g for 1 min then lysate was transferred to a 384-well plate (30 μL × 2 TR-FRET replicates) using an adjustable electronic multichannel pipette (Matrix Equalizer, ThermoFisher 2231) and TR-FRET measurements were taken ...
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bioRxiv - Biophysics 2021Quote: In vitro FRET experiments were conducted in black plastic 96-well plates (Nunc) with clear floors using a CLARIOstar plate reader (BMG LABTECH) ...
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bioRxiv - Cell Biology 2022Quote: ... Luminescent ATP Detection Assay Kit (based on firefly’s luciferase / luciferin) was purchased from Molecular Probes.
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bioRxiv - Neuroscience 2021Quote: ... we used a T7 endonuclease based method (GeneArtTM Genomic detection kit, Thermo Fisher Scientific, #A24372). Briefly ...
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bioRxiv - Biochemistry 2024Quote: ... Luminescent ATP Detection Assay Kit (based on firefly’s luciferase / luciferin) was purchased from Molecular Probes.
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bioRxiv - Cell Biology 2020Quote: INS-1 832/13 cells were grown in 35-mm dishes and transfected at 50-60% confluency with FRET-based biosensors (2.5 μg plasmid DNA) using Lipofectamine 2000 (6 μl) (Invitrogen). 24 hours post-transfection cells were plated in μ-slide 8 well glass bottom chamber (Ibidi ...
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bioRxiv - Immunology 2020Quote: ... cells were treated with 2 mM caspase-3/7 detection reagent (Fisher Scientific) for 30 min ...
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bioRxiv - Biochemistry 2022Quote: ... and 2 µM CellEvent™ Caspase-3/7 Green Detection Reagent (ThermoFisher Scientific). Images were captured automatically every two hours for 48 hours using the IncuCyte™ S3 Live-Cell Analysis Instrument (Essen BioScience) ...
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bioRxiv - Cell Biology 2023Quote: ... and treated with 2 µM Caspase-3/7 Green detection reagent (C10423, Invitrogen) along with the specified compounds ...
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Ferroportin Depletes Iron Needed for Cell Cycle Progression in Head and Neck Squamous Cell CarcinomabioRxiv - Cancer Biology 2022Quote: ... Real Time qRT-PCR was performed using SYBR-green based detection on a Quant Studio 3 real time PCR system using PowerUP Syber green MasterMix (ThermoFisher). Primers for GAPDH and beta-actin were used as endogenous controls to which RNA levels of target genes were normalized to ...
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bioRxiv - Immunology 2022Quote: ... Cells were counted again and resuspended in 3 × 106 cells/mL before adding CC4F-AM (LiveBLAzer FRET-B/G Loading Kit with CCF4-AM, ThermoFisher Scientific). Cells were incubated for 1 hour ...
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bioRxiv - Cancer Biology 2023Quote: ... CellEvent caspase-3/7 green detection reagent (C10423; Invitrogen; 1:1000) was used to measure apoptosis ...
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bioRxiv - Neuroscience 2023Quote: ... plates were rinsed with 2-3 ml of PBS (Gibco, #10010023) and treated with 0,5 mL ReLeSR (Stemcell technologies ...
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bioRxiv - Neuroscience 2021Quote: ... based medium with B27 (2%, Thermofisher) and GlutaMAX (2 mM ...
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bioRxiv - Neuroscience 2023Quote: ... based media with B27 (2%, Thermofisher) and GlutaMAX (2 mM ...
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bioRxiv - Bioengineering 2019Quote: ... medium was switched to a B27-based Retinal differentiation medium (BRDM) (DMEM/F12 (3:1) with 2% B27 (w/o vitamin A, ThermoFisher Scientific, USA), 1x NEAA and 1x AA) ...
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bioRxiv - Pharmacology and Toxicology 2020Quote: The assay procedure is based on the manufacturer’s protocol for LanthaScreen™ TR-FRET Thyroid Receptor beta Coactivator Assay (Invitrogen, PV4686) with slight ...
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bioRxiv - Developmental Biology 2019Quote: ... followed by ECL-based detection (Pierce ECL plus, Thermo Scientific, 32123). The following primary antibodies were used for Western blot analysis ...
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bioRxiv - Genetics 2021Quote: ... DNA was quantified using fluorescence-based detection on a Qubit (Thermofisher). Whole genome sequencing libraries were prepared at The Centre for Applied Genomics (The Hospital of Sick Children ...
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bioRxiv - Microbiology 2023Quote: ... Cells were then loaded with CCF4-AM for 2 hours at room temperature using the LiveBLAzer Fret-B/G Loading with CCF4-AM kit from Thermo Fisher. After 2 hours ...
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bioRxiv - Microbiology 2022Quote: ... coverslips were stained with anti-STING monoclonal antibody (Life Technologies, 1:100) in confocal blocking buffer overnight at 4°C ...
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bioRxiv - Neuroscience 2023Quote: ... and loaded with the Fluorescent Dye-Based Rhod-3 AM following the manufactures’ instructions (Rhod-3 Calcium Imaging Kit, Cat.No. R10145; ThermoFisher scientific). Loading ...
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bioRxiv - Immunology 2023Quote: ... a mouse IL-2 detection kit (Thermo Fisher #50-171-92) or mouse IFNγ detection kit (Thermo Fisher #88-7314-22 ...
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bioRxiv - Microbiology 2021Quote: ... or with 2 µM CellEvent™ Caspase-3/7 Green Detection Reagent (Thermo Fisher Scientific) for 30 min at room temperature (1:150 in AnnexinV binding buffer-Biolegend) ...
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bioRxiv - Neuroscience 2021Quote: ... frozen EVs were resuspended in RLT lysis buffer and RNAse-free water in a ratio of 1:3 and loaded based on protein concentration measured by Micro BCA Protein Assay Kit (Thermo Scientific). 2,8 μg of proteins were loaded.
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bioRxiv - Molecular Biology 2021Quote: ... cells were labeled with 5-ethynyl-2’-deoxyuridine followed by detection by click chemistry and then flow cytometry based on the instructions of the manufacturer (ThermoFisher, C01425).
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bioRxiv - Cancer Biology 2023Quote: ... or STING (cat. no. PA5-23381, Invitrogen) was done overnight at 4°C ...
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bioRxiv - Immunology 2020Quote: Cell viability was estimated by a quantitative colorimetric assay described for human granulocytes which were based on metabolic reduction of 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT, Invitrogen) into coloured product formazan (Oez ...
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bioRxiv - Cell Biology 2021Quote: ... Introduction of cGAMP was accomplished using Lipofectamine RNAiMax (ThermoFisher) as previously reported (Swanson et al. ...
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bioRxiv - Neuroscience 2019Quote: ... for 2 hours and detected using the EdU detection kit (Life Technologies) according to manufacturer’s instructions.
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bioRxiv - Cancer Biology 2020Quote: ... CellEvent Caspase-3/7 Detection Reagent (Invitrogen) and Hoescht were added to the media at concentrations listed in S4 Table and incubated for 30min at 37°C in 5% CO2 ...
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bioRxiv - Neuroscience 2023Quote: ... for chemical detection (Applied Biosystems; QuantStudio 3). Enrichment was determined based on double-delta CT value ...
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bioRxiv - Cell Biology 2019Quote: ... Detection of DNA synthesis in proliferating cells was determined based on the incorporation of 5-ethynyl-2′-deoxyuridine (EdU; Thermo Fisher Scientific) and its subsequent detection by a fluorescent azide through a Cu(I)-catalyzed [3 + 2] cycloaddition reaction (“click” chemistry ...
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bioRxiv - Biochemistry 2023Quote: The panel of synthetic peptide standards and the products of immunoprecipitated heparan-sulfate 6-O-sulfotransferase 1/2/3 (H6ST-1/2/3) in vitro Tyr sulfation assays were analyzed using Proteome Discoverer 2.4 (Thermo Scientific) in conjunction with MASCOT 59 against either a custom database of all (12 ...
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bioRxiv - Microbiology 2022Quote: Cell death assays were carried out using a staining protocol based on the eBioscience Annexin-V apoptosis detection kit PE and Fixable Viability Dye eFluorTM 780 (FVD; Invitrogen), following manufacturer’s recommendations.
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bioRxiv - Synthetic Biology 2024Quote: ... EdU detection was by Click chemistry-based AlexaFluor-488 flow cytometry assay (ThermoFisher). For RT-qPCR ...
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bioRxiv - Molecular Biology 2021Quote: ... a decrease in the FRET signal occurs and a lower TR-FRET ratio is detected (Invitrogen Corporation, 2008). For each compound (XN ...
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bioRxiv - Microbiology 2020Quote: ... CCF2-AM substrate (LiveBLAzer™ FRET-B/G Loading Kit with CCF2-AM, ThermoFisher) (31 ...
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bioRxiv - Neuroscience 2020Quote: ... Cells were then passaged 1:3-1:6 every 2-3 days using Accutase (Gibco).
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bioRxiv - Immunology 2020Quote: ... Detection of active caspase 3/7 was accomplished using CellEvent™ Caspase-3/7 Red Detection Reagent (ThermoFisher Scientific). Sorted CD4SP Rag1GFP+ thymocytes were stained with CD5-PerCP-Cy5.5 (53-7.3 ...
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bioRxiv - Cell Biology 2022Quote: The interaction between PAR-2 and GSP-1/-2 was assessed using a GAL4-based system (Gateway, Invitrogen) using the MAV203 yeast strain ...
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bioRxiv - Immunology 2019Quote: ... for 1h at 37°C or 2 μM CellEvant CASPASE-3/7 Green detection reagent (Thermo Fisher) for 30 minutes at 37°C ...