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Citations for GenScript :
351 - 400 of 457 citations for Rat TUT1 shRNA Plasmid since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Synthetic Biology 2021Quote: The human TDF HMG-box (hereinafter called TDF) was cloned into the plasmid pET-24c (+) (KanR) by GenScript (USA) using the sequence from the position 313 to 528 of the mRNA ...
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bioRxiv - Immunology 2020Quote: ... plasmids encoding cDNAs for the heavy and light chain sequences of 101F,64 MPE8,49 and DS747 were synthesized (GenScript), and cloned into vectors encoding human IgG1 and lambda or kappa light chain constant regions ...
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bioRxiv - Immunology 2021Quote: ... The full-length native chicken ovalbumin (OVA) gene inserted in a pcDNA3.1+ plasmid (clone ID: OGa28271) was purchased from GenScript. Gene inserts from pcDNA3.1+ plasmids were cloned into the pAM2AA backbone incorporating the liver-specific human α-1 antitrypsin promoter and human ApoE enhancer flanked by AAV2 inverted terminal repeats ...
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bioRxiv - Cancer Biology 2022Quote: pcDNA3.1+C-HA Trp53 (OMu22847) and pcDNA3.1+C-FLAG Ppia (OMu14516) plasmids used in mammalian over-expression experiments were purchased from Genscript. The detailed information regarding plasmid constructions is available by request ...
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bioRxiv - Bioengineering 2022Quote: ... by means of overhang PCR and was cloned via traditional restriction/ligation into a pET28a(+) plasmid (ordered from GenScript) coding for the C-terminal flexible linker and small BiT (SB ...
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bioRxiv - Microbiology 2022Quote: The pspA1 and pspA2 genes of SF35 were synthesized and cloned in pET-28a(+)-Tev plasmid (GenScript Biotech (Netherlands) BV) ...
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bioRxiv - Microbiology 2022Quote: ... SARS-CoV-2 spike constructs were generated and cloned into the pcDNA3.1 plasmid backbone using KpnI and BamHI restriction enzyme cloning by GenScript Biotech (Piscataway ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... thus introducing the cloning artifact ‘GPLGS’.37 The pGEX-6p-1-GST-OSBP377-807 plasmid was obtained from Genscript. The pET22b-His6-STARD166-284 plasmid was a gift from James H ...
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bioRxiv - Neuroscience 2023Quote: ... An IgG2b expression plasmid encoding the K89/34 IgG2b R-mAb was generated to order by Genscript (https://www.genscript.com/) by replacing the mouse IgG2a (ψ2a ...
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bioRxiv - Bioengineering 2023Quote: The L1 gene fragment sequences of human papillomavirus (HPV) types 16 and 18 were incorporated into the pCDNA3.1(+) plasmid by Genscript. To amplify the specific regions of interest ...
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bioRxiv - Cell Biology 2023Quote: DNA sequences used for plasmid construction were either amplified from the cDNA of HEK293T cells or purchased from GenScript and WZ Biosciences ...
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bioRxiv - Microbiology 2023Quote: ... The following plasmids were used in this study: FLAG tagged ORFs in pcDNA3.1+/C-(K)DYK were purchased from Genscript: CDK1 (NM_001786.5) ...
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bioRxiv - Cell Biology 2024Quote: ... The PLCε-FLAG-P2A-mCherry plasmid was synthesized and subsequently cloned into a pCMV-script EX vector by GenScript. For rescue experiments involving PLCε KO chromaffin cells ...
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bioRxiv - Plant Biology 2020Quote: ... the 500 bp 3’sequences from AT1G76560 (wild-type and mutated) and AT1G03160 flanked by attR1 and attR2 sites were synthesized and inserted in the pUC57 plasmid by Genscript. The DNA insert was then shuttled by Gateway cloning into the dual-luciferase vector nLucFlucGW (Genbank MH552885 ...
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bioRxiv - Synthetic Biology 2021Quote: ... and cloned into plasmid pBAD24 (79) between EcoRI and SphI restriction sites with an NdeI site at the start codon by GenScript. SNARES involving ClyA ...
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bioRxiv - Synthetic Biology 2021Quote: ... and 300–600 bp homology arms were synthesized as gBlocks (IDT) and cloned into a pUC19 plasmid inhouse or purchased directly from Genscript Biotech ...
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bioRxiv - Cancer Biology 2021Quote: ... XPA expression plasmids contain full-length human XPA (NM_000380) with the indicated mutations in the pcDNA3.1(+) backbone (GenScript custom order).
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bioRxiv - Cell Biology 2020Quote: ... The resulting intermediate 2 was complemented with a copia promoter-blasr cassette after amplifying the cassette using JB011 and JB012 from a plasmid with a corresponding synthetic DNA insert obtained from GenScript. The resulting PCR fragment was digested with NcoI and SpeI ...
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bioRxiv - Biochemistry 2022Quote: ... the expression plasmid encoding the sequence of 3D7 PfAMA1(DI+DII) (104-438) was obtained commercially from GenScript (NI, USA) having N-terminal hexa-His tag along with one TEV-protease cleavage site ...
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bioRxiv - Microbiology 2019Quote: Mammalian expression plasmids (pcDNA3.1+/C-(K)DYK) for HLA-DRA (NM_019111) and HLA-DRB1 (NM_001243965) were purchased from GenScript (Piscataway, NJ; USA). HEK293T/17 cells at sub-confluence in 6-well plates or 100 mm dishes were transfected with HLA-DRA plasmid or HLA-DRB1 plasmid or a 1:1 combination of both using the Lipofectamine 3000 transfection reagent (Thermo Fisher Scientific ...
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bioRxiv - Molecular Biology 2020Quote: ... The HNF4A-targeting gRNA is from the genome-scale CRISPR knock-out (GeCKO) v2 library [106] (purchased as lentiviral plasmid from Genscript) and the FMR1-targeting gRNA from the Jaenisch lab was obtained from Addgene (pgRNA-CGG ...
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bioRxiv - Biochemistry 2020Quote: ... The plasmid encoded an N-terminal 8X His-SUMO-tagged hNatD in the pET-21a vector was obtained from Genscript. Library of Pharmaceutically Active Compounds (LOPAC ...
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bioRxiv - Microbiology 2020Quote: ... were synthetized and cloned in plasmid pSG1-RFP (between the AscI and NotI sites of the multiple cloning sites) by Genscript Corp ...
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bioRxiv - Synthetic Biology 2021Quote: All gBlocks fragment containing 5 sgRNA expression cassettes with high fidelity four-base overhang pair2 after cutting with type IIS restriction enzyme BbsI restriction enzyme were designed and directly sent to be synthesized into PUC57 cloning plasmid by GenScript. Two oligos with BbsI cutting sites were annealed and cloned into backbone vector with CMV promoter drive fluorescent protein expression using SpeI-HF ...
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Comparison of the neuraminidase antigenicity in recently circulating influenza A and vaccine virusesbioRxiv - Microbiology 2021Quote: ... The genes corresponding the NAs from the following strains were synthesized and inserted into the pHW2000 plasmid [47] by Genscript: A/H1N1/Guangdong-Maonan/SWL1536/2019 (N1-GD19) ...
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bioRxiv - Systems Biology 2021Quote: ... The +1 nucleotide was mutated to all other nucleotides (G, C or T) and these 3 mutant plasmids were synthesized into DNA oligos and cloned by Genscript.
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bioRxiv - Cell Biology 2021Quote: ... the base mNeon-pDM304 expression plasmid for N-terminal fusions was generated by restriction enzyme cloning a codon-optimized synthesized mNeon gene (GenScript) into the extrachromosomal expression plasmid pDM304 (Veltman et al. ...
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bioRxiv - Cell Biology 2021Quote: ... The base Scarlet I-pDM304 expression plasmid for C-terminal fusions was generated by restriction enzyme cloning a codon-optimized synthesized Scarlet I gene (GenScript) into the extrachromosomal expression plasmid pDM304 (Veltman et al. ...
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bioRxiv - Biophysics 2021Quote: pET-DUET expression plasmids containing the genes for barnase and barstar under the control of their own T7 promoter were obtained from GenScript Biotech Corporation (Piscataway ...
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bioRxiv - Biophysics 2021Quote: A plasmid expressing mature OmpA without the 22 amino acid signal sequence in the pET303 vector was purchased from Genscript for cloning of the modified loop constructs ...
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bioRxiv - Bioengineering 2022Quote: ... knock-out was constructed by cloning a chloramphenicol marker to the pUC57 plasmid containing the sequences flanking acr1 (here named as pJL8, purchased from Genscript). The plasmid pJL10 was constructed by cloning the PT5-acr1-kanr cassette (Luo et al. ...
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bioRxiv - Biochemistry 2022Quote: The gene encoding SARS-CoV-2 NSP3 Mac1 (residues 3-169) was cloned into a pET-22b(+) expression plasmid with a TEV-cleavable N-terminal 6-His tag (Genscript). The protein was expressed and purified as described previously (14).
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bioRxiv - Immunology 2022Quote: ... Matched pairs of antibody VH and Vλ/Vκ sequences were commercially cloned into plasmids containing an IgG1 or relevant light chain backbone and expressed as recombinant antibody (Genscript). mAbs were also expressed in-house by transient transfection of Expi293 cells (Gibco ...
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bioRxiv - Neuroscience 2020Quote: TDP-43 BiFC constructs: Synthesis and subcloning of the gene fragments into pCS2+ plasmids were performed by GenScript (NJ, USA). Wild-type human TDP-43 was N-terminally fused to either VN155 or CC155 via a (GGGS)3 flexible linker ...
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bioRxiv - Microbiology 2021Quote: ... coli K-12 MG1655 thymidylate kinase alleles (WT, Q45P, and A69T) were amplified and cloned into the plasmid expression vector pRSFDuet-1 (GenScript). Expression is under control of the T7 lac promoter ...
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bioRxiv - Molecular Biology 2022Quote: ... HeLa FJ-KO cells were transfected with the following plasmids: pMK204-TetOne AAVS1-MCS (+) Flag-tagged FANCJ WT or pMK240-TetOne AAVS1-MCS (+) Flag-tagged FANCJ-AALA (GenScript). The plasmid pMK240-TetOne AAVS1-MCS (+) ...
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bioRxiv - Bioengineering 2019Quote: ... DNA sequences coding for HMG-box Pf and Hs were cloned into the plasmid pET-24c (+) (KanR) by GenScript (USA). The coding sequences of both domains were optimized for codon usage of the host (E ...
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bioRxiv - Microbiology 2020Quote: ... stable GFP expression by GAS was created by synthesizing the ribosomal binding site (RBS) and gfp gene from pDCerm-GFP (Ly et al., 2014) into the pUC57 plasmid (GenScript), resulting in pUC57-RBSGFP plasmid ...
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bioRxiv - Biophysics 2020Quote: The plasmid containing the catalytic domain of PKA (PKAc) was a gift from Susan Taylor via Addgene (#14921).13 The other plasmids were synthesized by Genscript and codon optimized for expression in E ...
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bioRxiv - Biophysics 2020Quote: ... The plasmid encoding full-length human DAP5 with an N-terminal 6x-histidine tag was purchased from Genscript (Piscataway, NJ). All the proteins were recombinantly expressed in E ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... SARS CoV-2 Mpro and PLpro expression plasmids pcDNA3.1 SARS2 Mpro and pcDNA3.1 SARS2 PLpro was ordered from Genscript (Piscataway NJ) with codon optimization.
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bioRxiv - Animal Behavior and Cognition 2021Quote: ... The latter plasmid was generated by placing the following de novo synthesized V5-GAL4v-VP48-OLLAS (GAL4-VP48) sequence (Genscript) into EcoRV-digested pUC57 ...
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bioRxiv - Synthetic Biology 2020Quote: ... pSensor09 was constructed by amplifying the backbone plasmid p416TEF1 using primer pair pYDA05/20 and primer pair pYDA21/22 to amplify PTEF1-YAS3-TCYC1 (Genscript_003).
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bioRxiv - Neuroscience 2019Quote: ... ZsGreen sequences were removed from the plasmid (HRI-HA Tet-On minZsGreen) leaving only HRI and HA inserted (constructs synthesized by GenScript). Codon-optimized versions were made in HRI-HA Tet-On minZsGreen plasmids using GenScript algorithms including optimization of the HA tag ...
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bioRxiv - Immunology 2020Quote: ... plasmid that contained predesigned guide RNA targeting Adar1 (5’-CTTGTCCGTCAAGTACCAGA-3’) and a pLentiCRISPR v2 plasmid that contained predesigned guide RNA targeting Adar2 (5’-AGTACCGCCTGAAGAAGCGA-3’) were obtained from GenScript. These plasmids were then transfected into Raw 264.7 cells using a Neon Transfection System (Thermo Fisher Scientific ...
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bioRxiv - Biochemistry 2021Quote: ... following published Current Protocols in Neuroscience.43 Plasmids for SARS-CoV-2 variants were synthesized using the prototype sequence (GenScript MC_0101081 ...
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bioRxiv - Immunology 2021Quote: ... were derived from the Wuhan-Hu-1 strain genome sequence (GenBank MN9089473) and synthesized and subcloned into a CMVR plasmid by Genscript. RBD with VOC point mutations were generated using a modified QuikChange site-directed mutagenesis protocol (Agilent) ...
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bioRxiv - Immunology 2020Quote: The SARS-CoV-2 pseudovirus was produced by co-transfection of HEK293T cells with 1:1 ratio of DNA plasmid encoding SARS-CoV-2 S protein (GenScript) and backbone plasmid pNL4-3.Luc.R-E-(NIH AIDS Reagent ...
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bioRxiv - Molecular Biology 2021Quote: ... plasmid (Addgene plasmid #48138)62 containing a guide RNA (5’-ACTGAGCTTGGATGCTTCTG-3’) and a donor plasmid containing 700 bp homology arms and a RPAC1 tag synthesised by GenScript into pUC57-Mini plasmid ...
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bioRxiv - Genetics 2022Quote: ... integration cassettes were constructed by replacing a spt5+ gene fragment (+2398 of the spt5 ORF to the stop codon) in the pUC19-based spt5CTD+-ura4-spt53’ plasmid (75) with synthetic DNA fragments (Genscript) harboring the relevant mutations ...