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Citations for GenScript :
201 - 246 of 246 citations for DNA Standards since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2022Quote: ... This synthetic DNA was cloned into the DIs vector plasmid pSMART-DIs-L3-GPTF (purchased from GenScript, Nanjing, China), which harbors the Escherichia coli gpt gene (encoding xanthine-guanine phosphoribosyltransferase ...
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bioRxiv - Biochemistry 2021Quote: DNA polynucleotides encoding the opsin domains optimized for human codon usage were synthesized and cloned by GenScript (Piscataway, NJ) into the mammalian expression vector pcDNA3.1 (Life Technologies ...
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bioRxiv - Genetics 2023Quote: ... mutans cultures were diluted 1:40 from overnight cultures and grown to an optical density of OD600 ∼0.1 in THYE before the addition of transforming DNA and 1 μg ml−1 Competence Stimulating Peptide (CSP; GenScript). The cultures were subsequently incubated for an additional 2 h and then plated on antibiotic-supplemented THYE plates ...
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bioRxiv - Biochemistry 2023Quote: The DNA sequence of pancreatic human GCK (Ensembl ENST00000403799.8) was codon optimized for yeast and cloned into pDONR221 (Genscript). Selected missense variants were generated by Genscript ...
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bioRxiv - Cell Biology 2023Quote: DNA sequences used for plasmid construction were either amplified from the cDNA of HEK293T cells or purchased from GenScript and WZ Biosciences ...
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bioRxiv - Genomics 2024Quote: The two recoded TALEN arms were ordered as synthetic pieces of DNA delivered into the pUC57-Mini vector (GenScript). The left recoded arm was cloned in pHR’CMVGFP_hRIF1(1924-2446)IREShygro3 (Addgene #23138 ...
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bioRxiv - Cell Biology 2020Quote: ... A synthetic EcoRI-HindIII DNA fragment inserted into the corresponding restriction sites of pUC57 was obtained from GenScript (Leiden, Netherlands). The insert DNA of this intermediate 1 contained the minimal attB target sites for the PhiC31 and Bxb1 integrases as well as the mEGFP coding sequence ...
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bioRxiv - Cell Biology 2020Quote: ... The resulting intermediate 2 was complemented with a copia promoter-blasr cassette after amplifying the cassette using JB011 and JB012 from a plasmid with a corresponding synthetic DNA insert obtained from GenScript. The resulting PCR fragment was digested with NcoI and SpeI ...
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bioRxiv - Neuroscience 2021Quote: ... The DNA sequences for the APEX2-FUS variants were designed in silico then codon optimized and custom synthesized by GenScript. The amino acid sequence for the engineered APEX2 was taken from Addgene plasmid #212574 ...
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bioRxiv - Microbiology 2019Quote: ... Three de novo synthesized DNA fragments cloned into a pUC57 vector were used in this study (GenScript, Piscataway, NJ, USA): fragment I (nucleotide position 1 to 3662) ...
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bioRxiv - Biophysics 2020Quote: The DNA sequence coding for hGHR-ECD (1-245, C242S, no signal peptide) in a pET11a was bought from Genscript and transformed into competent Rosetta2 (DE3)pLysS cells ...
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bioRxiv - Bioengineering 2020Quote: ... was synthesized as gBlocks Gene Fragment from Integrated DNA Technologies Inc. The ATR2 gene (codon optimized for E. coli) was synthesized from GenScript. All strains and plasmids used in this study are listed in Table 1 and 2 ...
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bioRxiv - Systems Biology 2021Quote: ... The +1 nucleotide was mutated to all other nucleotides (G, C or T) and these 3 mutant plasmids were synthesized into DNA oligos and cloned by Genscript.
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bioRxiv - Biophysics 2020Quote: ... Synthetic DNAs were purchased as GeneBlocks from IDT (5’ leader constructs) or as Gene Parts from GenScript (5’UTR constructs). All synthetic DNAs had a 5’-terminal XmaI consensus sequence and 3’-terminal HindIII consensus sequence ...
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bioRxiv - Bioengineering 2019Quote: ... DNA sequences coding for HMG-box Pf and Hs were cloned into the plasmid pET-24c (+) (KanR) by GenScript (USA). The coding sequences of both domains were optimized for codon usage of the host (E ...
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bioRxiv - Molecular Biology 2021Quote: ... Roll-your-own-structure’ challenge were ordered in the form of a custom oligonucleotide pool of DNA (Custom Array/Genscript) with the 20-nt T7 RNA polymerase promoter sequence (5’-TTCTAATACGACTCACTATA-3’ ...
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bioRxiv - Microbiology 2019Quote: ... followed by the addition of 1μg of DNA along with 125μg/mL of CSP1 (sequence: EMRLSKFFRDFILQRKK; purchased from GenScript, NJ, USA). These treated cultures were incubated at at 37°C for 2 hours ...
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bioRxiv - Biochemistry 2021Quote: ... The codon-optimized gene encoding for these residues plus an engineered DNA sequence encoding for a C-terminal tobacco etch virus (TEV)-protease cleavage site (ENLYFQS) was synthesized by GenScript. This gene was then subcloned into the ampicillin-resistant ...
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bioRxiv - Immunology 2020Quote: The SARS-CoV-2 pseudovirus was produced by co-transfection of HEK293T cells with 1:1 ratio of DNA plasmid encoding SARS-CoV-2 S protein (GenScript) and backbone plasmid pNL4-3.Luc.R-E-(NIH AIDS Reagent ...
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bioRxiv - Molecular Biology 2021Quote: ... Variants of CMV-ubi-nsP4-RRV and CMV-ubi-nsP4-SINV containing mutations listed in supplementary figure S6 were constructed using synthetic DNA fragments (Genscript). Sequences of all plasmids were verified using Sanger sequencing and are available from the authors upon request.
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bioRxiv - Genetics 2022Quote: ... integration cassettes were constructed by replacing a spt5+ gene fragment (+2398 of the spt5 ORF to the stop codon) in the pUC19-based spt5CTD+-ura4-spt53’ plasmid (75) with synthetic DNA fragments (Genscript) harboring the relevant mutations ...
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bioRxiv - Synthetic Biology 2022Quote: ... The resulting chimeric DNA sequence was flanked a 3’-BamHI and 5’-EcoRI sites and commercially synthesised (GenScript, Rijswijk, Netherlands) into vector pUC19 (pJGUC01) ...
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bioRxiv - Molecular Biology 2022Quote: DNA encoding aa 403-1390 of ATAD2 was PCR amplified from the full-length codon-optimized synthetic ATAD2 gene (Genscript), and subcloned into an in-house modified pFastBac1 vector with an N-terminal 3xFlag tag and Tev protease cleavage site using EcoRI and XhoI restriction sites ...
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bioRxiv - Genomics 2023Quote: ... DNA pull-downs were performed as described in 29 with 10 ug of DNA oligo for each pull-down (unmodified, methylated, and hydroxymethylated; GenScript) and 400 ug of nuclear extract ...
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bioRxiv - Molecular Biology 2023Quote: ... NM_003755) with an N-terminal His6 tag was made by inserting DNA between NdeI and XhoI sites of pET15b (GenScript). pET15b-His6-eIF3g was used by GenScript to generate the deletion and substitution mutants.
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bioRxiv - Molecular Biology 2023Quote: The DNA sequences of all smORF proteins were synthesized and subcloned into a modified pRSET vector by GenScript (Hong Kong). The construct was tailored to have an N-terminal hexa-histidine-tagged lipoyl fusion protein followed by a thrombin cleavage site and the respective smORF protein ...
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bioRxiv - Immunology 2023Quote: ... DNA encoding HLA-C*05:01 (1-278) and β2M (1-99) were synthesized and cloned into pET30a by Genscript and were previously described42 ...
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bioRxiv - Plant Biology 2023Quote: ... and DAO1 DNA sequences (Saidi et al. 2005; Boute et al. 2016; Erikson et al. 2004) were synthesized by GenScript Biotech (https://www.genscript.com ...
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bioRxiv - Biochemistry 2023Quote: ... the gene was inserted between the BamHI and EcoRIsites of the vector pGEX-4T-1 with an N-terminal GST tag followed by an HRV 3C protease cleavage site. The DNA sequence encoding yeast (S. cerevisiae) Ubc4 was synthesized by GenScript and its codon was optimized for E ...
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bioRxiv - Biochemistry 2023Quote: Human full-length wild-type DNA Pol β was overexpressed from a PET-28a codon optimized clone purchased from GenScript in the BL21-CodonPlus(DE3)-RP E ...
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bioRxiv - Microbiology 2021Quote: ... and (green) were obtained by ligation between KpnI and BamHI of pcDNA-VP4-KpnI/BamHI a synthetic DNA fragment (GenScript®) containing BAP tag in VP4 loops in amino acid regions 96-101 ...
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bioRxiv - Immunology 2022Quote: ... The TCR variable region in combination with mouse TCR α and β constant regions was then obtained as synthetic DNA (GenScript) that was subcloned into the pMIG II vector and used for transduction36.
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bioRxiv - Biochemistry 2023Quote: The coding sequence of the 11 subunits of mouse CMG were codon optimised for high-level expression in budding yeast cells and produced by DNA synthesis (GenScript Biotech). A previously described ‘yeast toolkit’ (Lee et al. ...
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bioRxiv - Molecular Biology 2023Quote: ... AAP13442.1) with N-terminal His6 tags were made by inserting DNA between NcoI and BamHI sites of pET15b (GenScript, Piscataway, NJ). pET15b-His6-Nsp1(SARS CoV2 ...
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bioRxiv - Immunology 2023Quote: ... DNA template containing loxP sites flanking exon 2 of the Sparcl1 “loxP-Sparcl1_Ex2-loxP” was synthesized by Genscript (Genscript Biotech Corp.) and purified ...
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bioRxiv - Microbiology 2023Quote: ... or with the mutated or truncated cdeA promoter regions were made by either PCR amplification or by DNA synthesis (Genscript, USA). The DNA products were made with incorporated kpnI or xbaI enzyme recognition sites and were digested with KpnI and XbaI and ligated to KpnI-XbaI-digested pMP220 [15] upstream of the lacZ reporter ...
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bioRxiv - Microbiology 2023Quote: ... Fusion proteins were generated for the assay by cloning chlamydial DNA sequences encoding CPAF into a pET30a vector (constructed by Genscript Biotech), resulting in fusion proteins with a hexahistidine (His6 ...
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bioRxiv - Biochemistry 2024Quote: ... The DNA encoding each of these constructs was commercially synthesized and cloned in pET15b vector at NcoI and BamHI sites (GenScript, Inc) and then transformed into Bl21(DE3 ...
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bioRxiv - Molecular Biology 2020Quote: ... (referred to as eRF3 in the text) was made by inserting DNA between BglII and Nde1 sites of pET23(b) (GenScript, Piscataway, NJ).
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bioRxiv - Biochemistry 2020Quote: ... DNA polynucleotides encoding the transmembrane domains showing homology to previously known ACRs optimized for human codon usage were synthesized (GenScript, Piscataway, NJ) and cloned into the mammalian expression vector pcDNA3.1 (Life Technologies ...
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bioRxiv - Microbiology 2021Quote: ... auris ADH1 gene (B9J08_004331) was PCR amplified using primers oTO118-oTO119 and assembled along with a synthesized DNA fragment (Genscript, Piscataway, NJ, USA) containing sequence from C ...
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bioRxiv - Synthetic Biology 2022Quote: DNA chunks comprising ∼5-10 Kb of each megachunk were synthesized and sequence verified by Genscript (megachunks A-K and N-X), GeneArt (megachunks L ...
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bioRxiv - Genetics 2023Quote: ... Plasmids pML107 11 or PAEF5 12 carrying gRNA were co-transformed with 200bp DNA repair recombinant donor sequences carrying telomeric repeats seeds (Genscript Biotech Netherlands) (Figure Supp 1A).
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bioRxiv - Pharmacology and Toxicology 2022Quote: ... The DNA fragment encoding the protein was codon optimized for Escherichia coli (E. coli) protein expression and synthesized synthetically by Genscript (New Jersey, USA) before being cloned into the pMALc5x vector ...
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bioRxiv - Biophysics 2022Quote: DNA fragments coding the C-terminal 350 amino acids of E6AP were synthesized as a codon-optimized artificial gene (GenScript, Piscataway, NJ, USA). The products were subsequently ligated into the pET28a vector with NdeI and BamHI restriction enzymes and designated E6APHECT_WT ...
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bioRxiv - Cancer Biology 2020Quote: ... digested the vector with SpeI and SalI and introduced the following elements as a pre-synthetized stretch of DNA (GenScript®, Piscataway, NJ, USA): SpeI - SFFV - lox2272 - mtagBFP (iRFP720 ...