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Citations for Singer Instruments :
101 - 150 of 218 citations since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Molecular Biology 2023Quote: ... 100 µL of saturated cultures were dispensed into a sterile 96-well plate and subsequently pinned to 384 density on YPG agar using a Singer RoToR colony pinning robot (Singer Instruments). Plates were grown at room temperature until colonies formed (24-48 hours) ...
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bioRxiv - Genetics 2023Quote: ... Both assays take advantage of the RoToR HDA colony-pinning robot (Singer Instruments), which can pin out yeast on agar plates in a 96-well arrangement ...
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bioRxiv - Microbiology 2023Quote: ... then imaged using a Phenobooth imaging system (Singer Instruments) using the transmissive light mode ...
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bioRxiv - Evolutionary Biology 2023Quote: ... Treated cells were then placed on a level YPD plate to perform tetrad dissection with the SporePlay (Singer Instrument). The dissection plates were incubated at room temperature for 5 days to make sure we didn’t underestimate growth ...
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bioRxiv - Genetics 2023Quote: ... The serially diluted droplets were pinned in quadruplicate (384-well format) onto YES agar plates using a Singer RoToR HDA pinning robot (Singer Instruments). The plates were incubated at 32°C for 2-4 days until colonies were clearly visible ...
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bioRxiv - Evolutionary Biology 2023Quote: ... 63 random spores were dissected on Yeast Extract with Supplements (YES) agar plates (Petersen & Russell, 2016) using a Singer tetrad dissector (Singer Instruments).
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bioRxiv - Synthetic Biology 2022Quote: A PIXL colony picker (Singer Instruments, Roadwater, United Kingdom) was used to select individual colonies from the transformation plates using the 490-510 nm (cyan ...
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bioRxiv - Cell Biology 2022Quote: ... Cells were then moved using a microdissection needle (Singer Instruments) to a unique position on the plate ...
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bioRxiv - Cell Biology 2022Quote: ... we used a RoToR benchtop colony arrayer (Singer Instruments). In short ...
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bioRxiv - Molecular Biology 2022Quote: Using the benchtop RoToR HDA robotic plate handler (Singer Instruments Co Ltd, Roadwater, UK) strains were transferred from glycerol stocks to solid rich medium plates (YPDA ...
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bioRxiv - Bioengineering 2022Quote: ... Fluorescent mVenus expression intensities were analyzed by picking primary transformant colonies using a PIXL robot (Singer Instruments, UK) to 384 colonies/plate layout on manufacturer supplied rectangular Petri dishes ...
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bioRxiv - Evolutionary Biology 2022Quote: ... Yeast populations were subsampled and transferred to fresh plates by robotic pinning (ROTOR HDA, Singer Instruments, UK).
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bioRxiv - Molecular Biology 2022Quote: ... Pinning transfers were performed using 1536 short pin pads from the same production batch (Singer Instruments, UK), and a Singer RoToR HDA robot (Singer Instruments ...
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bioRxiv - Molecular Biology 2022Quote: ... and a Singer RoToR HDA robot (Singer Instruments, UK). All transfers were performed using a single robot ...
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bioRxiv - Molecular Biology 2022Quote: ... Solid media was cast in polystyrene plates from a single batch (Singer Instruments, SBS-format PlusPlates). Each plate was cast on top of the same perfectly level surface with precisely 50 mL of synthetic grape must medium at 50 C ...
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bioRxiv - Biochemistry 2022Quote: ... in quadruplicate (384-well format) using a Singer RoToR HDA pinning robot (Singer Instruments). For this ...
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bioRxiv - Cell Biology 2022Quote: ... Tetrad dissections were performed using an MSM-400 dissection scope (Singer Instruments, Somerset, United Kingdom) with 25µm needle following a one-week incubation at 26°C in standard sporulation media (1% yeast extract ...
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bioRxiv - Cell Biology 2022Quote: ... and libraries were handled using a RoToR bench-top colony arrayer (Singer Instruments). Briefly ...
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bioRxiv - Cell Biology 2022Quote: Cells were moved from agar plates into liquid 384-well plates using the RoToR bench-top colony arrayer (Singer Instruments). Liquid cultures were grown overnight in synthetic medium with 2% glucose (SD ...
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bioRxiv - Microbiology 2022Quote: ... after which spores from the different parental strains were dissected and placed next to each other on YPD agar plates (1 % yeast extract, 2 % peptone, 2 % glucose, and 2 % agar) using a micromanipulator (Singer Instruments, UK). The plates were incubated at 25 °C for 3 days ...
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bioRxiv - Microbiology 2022Quote: ... ascospores were digested using Zymolyase 100T (US Biological, Salem, MA, USA) and dissected on YPD agar using the MSM400 dissection microscope (Singer Instruments, Roadwater, UK).
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bioRxiv - Genomics 2022Quote: ... MA lines were pre-cultured in YPD broth and pinned onto a solid YPD plate to a 1536 density format using the replicating ROTOR robot (Singer Instruments). The plates were incubated for 24 hours at 30°C and were scanned with a resolution of 600 dpi at 16-bit grayscale ...
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bioRxiv - Genetics 2022Quote: ... the query strains were crossed with the array ncRNA deletion library in the 1536 format on YPD agar using a RoToR HDA robot and disposable plastic replicators (pinning RePads, Singer Instruments, UK). SGA was carried out according to Baryshnikova ...
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bioRxiv - Genetics 2022Quote: ... The images of these plates were recorded using a Phenobooth (Singer Instruments) for subsequent SGA analyses.
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bioRxiv - Genetics 2022Quote: ... The library was first organised in 96 and 384 density formats and subsequently used as a source to generate the final working copy of two Plus Plates (Singer Instruments, UK) in a density of 1536 colonies per plate using the RoToR HDA system (Singer Instruments ...
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bioRxiv - Genetics 2022Quote: ... in a density of 1536 colonies per plate using the RoToR HDA system (Singer Instruments, UK). Each single deletion strain was plated in quadruplicates allowing replicates for consistent SGA analysis ...
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bioRxiv - Cell Biology 2022Quote: Cells were moved from agar plates into liquid 384-well plates using a RoToR bench-top colony arrayer (Singer Instruments). Liquid cultures were grown overnight in low biotin 0.512nM d-biotin (sigma ...
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bioRxiv - Cell Biology 2022Quote: The lysate was blotted to a nitrocellulose membrane (pall) using the RoToR into a 1536 dot array using the 384 long pads (Singer Instruments) calibrated to move 200pL of liquid ...
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bioRxiv - Systems Biology 2022Quote: ... Plates were replica plated with an automated RoToR HDA system (Singer Instruments).
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bioRxiv - Microbiology 2022Quote: ... colonies were pinned on the antibiotic-containing plates and once on a vehicle control plate at 1536 colony density using the ROTOR HDA (Singer Instruments). This experiment was done in duplicate using two different source plates ...
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bioRxiv - Cell Biology 2022Quote: ... Yeast manipulations in high-density format were performed on a RoToR bench top colony arrayer (Singer Instruments). In short ...
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bioRxiv - Cancer Biology 2022Quote: ... and pinned in quadruplicate to achieve a 384-colony density (i.e. 4 technical replicates for each position of the microtiter plate) using a Singer RoToR robot (Singer Instruments, Inc. Somerset UK). Strains were grown on YES solid agar media with the indicated camptothecin concentrations ...
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bioRxiv - Genetics 2022Quote: ... and a gene deletion were performed by sequential pinning of yeast colonies on appropriate selective media using a RoToR pinning robot (Singer Instruments) as described (Baryshnikova et al. ...
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bioRxiv - Evolutionary Biology 2022Quote: ... 2.20.504.1 (Singer Instruments) at 2560 × 1920 pixel resolution under default lighting settings.
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bioRxiv - Evolutionary Biology 2022Quote: ... The liquid cultures were briefly resuspended by pipetting and arrayed in a 384-position configuration on CM plates (2% w/v agar, 50 mL/plate) using a ROTOR HDA (Singer Instruments). These plates were grown for 2 days at 30°C ...
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bioRxiv - Synthetic Biology 2022Quote: ... Tetrad Dissection was performed using Spore play (Singer Instruments for every engineered proteasome spore on YPD and then replicated on SGA selection ...
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bioRxiv - Microbiology 2022Quote: ... or the PhenoBooth instrument (Singer Instruments, UK).
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bioRxiv - Microbiology 2022Quote: ... was examined using the PhenoBooth instrument (Singer Instruments, UK) and belonging software ...
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bioRxiv - Microbiology 2022Quote: ... using the ROTOR HDA (Singer Instruments, UK). When YPD solid medium was used ...
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bioRxiv - Microbiology 2022Quote: ... incubated overnight in yeast extract peptone dextrose (YPD) in 96-well plates and inoculated onto solid YPD and spider media using a 96-pin replicator tool (Singer Instruments). Plates were then incubated at the indicated temperature and oxygen conditions for 7-9 days ...
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bioRxiv - Plant Biology 2022Quote: ... The CLiP library was replicated using a Singer RoToR robot (Singer Instruments, 704) on 384-format 1.5% TAP agar plates and grown in the dark ...
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bioRxiv - Molecular Biology 2022Quote: ... a RoToR array pinning robot (Singer Instruments) was used to mate the parental N’ tag GFP SWAT library with the required donor strain (Table S2 ...
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bioRxiv - Microbiology 2022Quote: ... Meiotic segregants were obtained by dissecting tetrad ascospores treated with 10 µL Zymolyase 100T (50 mg/mL) in a SporePlay micromanipulator (Singer Instruments, UK) and crossed on YPD agar plates ...
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bioRxiv - Synthetic Biology 2022Quote: ... Digested cells were plated onto a YPD plate and tetrads were dissected to individual spores arrayed on the plate using a SporePlay+ tetrad dissection microscope (Singer Instruments). Mating type of isolated haploids was determined by PCR as previously described (Huxley et al. ...
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bioRxiv - Cell Biology 2022Quote: ... Transformations were performed as described in [173] and were done with the aid of the Benchtop RoToR HAD robotic plate handler (Singer Instruments Co. Ltd, Roadwater, UK) and the Multidrop Combi (ThermoFisher Scientific ...
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bioRxiv - Synthetic Biology 2022Quote: ... Images were taken every 24 h using the PhenoBooth+ (Singer Instruments).
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bioRxiv - Synthetic Biology 2022Quote: ... 10-fold dilution series were prepared in 96-well microtiter plates and the Rotor HDA+ (Singer Instruments) was used to perform 7x7 grit pinning on the described solid media ...
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bioRxiv - Cell Biology 2022Quote: ... a defined numbers of spores were dissected on YES plates using a Singer MSM micromanipulator (Singer Instruments). Between 50 and 100 spores per biological replicate were dissected ...
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bioRxiv - Synthetic Biology 2021Quote: ... each individual colony was isolated using the Rotor HDA colony picker (Singer instruments, UK). Using the colony picker ...
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bioRxiv - Genetics 2021Quote: ... Strains were arranged into 384-colony format using a RoToR HDA colony-pinning robot (Singer Instruments), including a 96-colony grid of wild-type 972 h- strains for plate normalization (Kamrad ...