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Citations for Singer Instruments :
1 - 50 of 218 citations since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Molecular Biology 2024Quote: ... Ascus walls were digested for 20 minutes with β-glucuronidase and spores were then separated using a dissection microscope (Singer Instruments). After growth on a YPD plate for two days at 30°C ...
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bioRxiv - Microbiology 2024Quote: ... Colonies were picked either using a disposable loop or a PIXL colony picking robot (Singer Instruments, PIX-001). When picking by hand ...
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bioRxiv - Microbiology 2024Quote: ... and spores of opposite species were dissected and placed next to each other on a YPD agar plates using a SporePlay micromanipulator (Singer Instruments, UK). Plates were incubated at two different temperatures ...
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bioRxiv - Microbiology 2024Quote: ... The PhenoBooth (Singer Instruments, UK) was used to capture images every 10 h ...
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bioRxiv - Genetics 2024Quote: We obtained all the colony size of the mutants using the Phenobooth (Singer Instruments Ltd, Somerset, UK). The colony sizes of both double and single mutants were normalized per plate ...
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bioRxiv - Genetics 2024Quote: ... The tetrads were separated/dissected using a Singer micromanipulator (Singer instruments, UK) on YPAD-G418 (300 mg/L ...
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bioRxiv - Genetics 2024Quote: ... The query strains were mated with the library mutant strains to create double mutants in 384-well format with a RoToR HDA pinning robot (Singer Instruments) in EMM-N-agar supplemented with adenine ...
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bioRxiv - Genomics 2024Quote: ... was crossed with strains harboring the ura4+ reporter gene at different heterochromatic loci by using RoToR HDA colony pinning robot (Singer Instruments). To enable selection of the ura4+ reporter under conditions of transcriptional repression ...
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bioRxiv - Genetics 2024Quote: The plates were imaged with the PhenoBooth Colony Counter (Singer Instruments, UK) after 72 hours of incubation and the size of the individual colonies was used as a proxy for fitness ...
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bioRxiv - Genetics 2024Quote: ... and in PlusPlates (Singer Instruments, UK) with YPD + 2% agar incubated at 30°C.
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Recurrent loss of crossover interference punctuates the recombination landscape across yeast speciesbioRxiv - Genomics 2024Quote: ... Tetrads dissections were performed using the SporePlay (Singer Instrument) without any pre-treatment ...
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bioRxiv - Cell Biology 2024Quote: ... Cells were moved from agar plates into liquid 384-well polystyrene growth plates using the ROTOR HDA (Singer Instruments). Liquid cultures were grown overnight in 50 μl of SD medium in a shaking incubator (LiCONiC Instruments ...
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bioRxiv - Microbiology 2024Quote: ... The yeast strains were revived from –80 °C in YPD broth and arranged in 3×4 squares (Fig. S1A) on SBS PlusPlates containing solidified 12 °Bx wort and YPD using the PIXL (Singer Instruments, UK) robotic platform ...
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bioRxiv - Microbiology 2024Quote: ... A volume of 45 mL of media was poured into SBS PlusPlates (Singer Instruments, UK) and left to set on an even surface.
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bioRxiv - Microbiology 2024Quote: ... The plates were replicated onto agar YPD and solidified wort plates using a ROTOR HDA (Singer Instruments, UK), sealed and incubated at 20 °C for 210 h ...
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bioRxiv - Synthetic Biology 2024Quote: ... colony counts were obtained to determine total library size and plates were imaged for fluorescence using GFP imaging conditions on a Phenobooth+ (Singer Instruments) system (excitation LED ...
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bioRxiv - Synthetic Biology 2024Quote: ... library transformants on solid media were imaged for fluorescence using GFP imaging conditions on a Phenobooth+ (Singer Instruments) system (excitation LED ...
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bioRxiv - Systems Biology 2024Quote: We performed eY1H assays using a human TF yeast array (15) as previously described and as follows using a high-density array ROTOR robot (Singer Instruments). The three-plate human TF yeast array and promoter yeast strains were mated pairwise on permissive media agar plates and incubated at 30°C for 1 day ...
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bioRxiv - Systems Biology 2024Quote: ... Screening of TF-pairs and cancer gene promoters was performed similarly to eY1H screens as previously described and as follows using a high-density array ROTOR robot (Singer Instruments). The five-plate TF-pair yeast array and promoter yeast strains were mated pairwise on permissive media agar plates and incubated at 30°C for 1 day ...
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bioRxiv - Systems Biology 2024Quote: We performed eY1H assays as follows using a high-density array ROTOR robot (Singer Instruments). Yeast strains were arrayed such that all 18 ESR1 constructs and two empty control yeast strains were tested against 10 different promoters in each 1,536-colony agar plate ...
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bioRxiv - Evolutionary Biology 2024Quote: ... This suspension was then transferred to a petri dish and subjected to either 0 seconds (mock mutagenesis) or 10 seconds of UV-C radiation in a Singer Rotor HDA (Singer Instruments, Somerset, England). The cell suspension was then moved back to a 15 mL conical tube and spun down at 700 rcf for five minutes ...
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bioRxiv - Cell Biology 2024Quote: ... The handling of libraries was facilitated using a RoToR bench-top colony array instrument (Singer Instruments). In brief ...
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bioRxiv - Evolutionary Biology 2024Quote: ... Replica plating was performed using a Singer Rotor HDA robot (Singer Instruments). For copper we used 18 concentrations ranging from 0 mM to 0.8 mM and for sulfite we used 16 concentrations ranging from 0 mM to 6 mM for haploids and 21 concentrations ranging from 0 mM to 6 mM for diploids ...
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bioRxiv - Evolutionary Biology 2024Quote: Colony size was measured using images obtained in a Singer Phenobooth using the Phenosuite software package (Singer Instruments). Colony size measurements were performed at 24 hour intervals for three days for copper and permissive conditions ...
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bioRxiv - Synthetic Biology 2024Quote: ... The Rotor (Singer Instruments) played a crucial role in managing arrays of transformants ...
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bioRxiv - Cell Biology 2024Quote: ... Weill et al, 2018) using automated mating and selection procedures (Tong & Boone, 2007; Cohen & Schuldiner, 2011) and robotic arrayer Rotor HDA (Singer Instruments). In brief ...
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bioRxiv - Cell Biology 2024Quote: ... The steps of this processes were done using a RoToR bench-top colony arraying instrument (Singer Instruments, UK). For the BMV 1aHB-GFP screen that is described in Figure 1 ...
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bioRxiv - Evolutionary Biology 2024Quote: ... Cells were spot diluted on each medium as described above and imaged using a Phenobooth following three days of growth (Singer Instruments). YJF5535 (S ...
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bioRxiv - Synthetic Biology 2024Quote: ... Data was documented with the PhenoBooth (Singer Instruments, Somerset, UK). The determined length library for RybB and SgrS of constitutively expressed synthetic sRNA constructs was performed based on manual dilution series of indicated overnight cultures generated in 96-well or 384-well flat bottom microtiter plates using multichannel pipettes ...
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bioRxiv - Cell Biology 2024Quote: ... All pinning steps for collection handling were performed using a SINGER ROTOR HDA robot (Singer Instrument, Watchet UK).
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β-1,6-glucan plays a central role in the structure and remodeling of the bilaminate fungal cell wallbioRxiv - Microbiology 2024Quote: ... 72 h and 6 days with a Phenobooth+ Colony counter (Singer Instruments). To observe macroscopic filamentation ...
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bioRxiv - Microbiology 2024Quote: Plus plates (Singer Instruments, cat. no. PLU-003) were prepared for Singer experiments following the protocol described in French et al. ...
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bioRxiv - Microbiology 2024Quote: ... The rotor used 384 long-pin pads (Singer Instruments, cat. no. REP-003) to conduct a robotic overlay where the bacterial culture was stamped first ...
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bioRxiv - Synthetic Biology 2024Quote: A PIXL robot (Singer Instruments) facilitated semi-automatic colony picking across various array formats ...
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bioRxiv - Synthetic Biology 2024Quote: ... The Rotor HDA+ (Singer Instruments, UK) was used to pin the dilution series in a 7×7 grid on selective media on a single-well microtiter plate ...
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bioRxiv - Molecular Biology 2024Quote: ... and subjected to tetrad analyses on YEPD plates using a Singer MSM400 micromanipulator (Singer Instruments, Somerset, UK). Plates were incubated for 3-5 days at 30°C as required for the specific mutant combinations ...
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bioRxiv - Microbiology 2024Quote: ... Precultures were grown overnight and diluted to an OD600 nm of 1 (WT) or 0.1 (deletion backgrounds) and used to inoculate 384 well plates using a Singer Rotor+ replicator (Singer Instruments, UK), resulting in further ∼1:600 dilution and starting OD of ∼0.003 and ∼0.0003 ...
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bioRxiv - Molecular Biology 2024Quote: The high-throughput replica-pinning screen was performed essentially as previously described (36) using a ROTOR-HDA pinning robot (Singer Instruments). In brief ...
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bioRxiv - Cell Biology 2024Quote: ... Tetrads were selected by using a tetrad micro dissector microscope (MSM400, Singer Instruments), to place 1 whole tetrad at each position ...
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bioRxiv - Synthetic Biology 2024Quote: ... Transformation plates were documented using the PhenoBooth (Singer Instruments, UK) using standard settings ...
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bioRxiv - Synthetic Biology 2024Quote: ... Documentation was performed using the PhenoBooth (Singer Instruments, UK) using the following settings ...
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bioRxiv - Systems Biology 2024Quote: The AID-v2 library was assembled in 1536-colony format on SC(MSG)-Leu + hygromycin/clonNAT plates using a pinning robot (Rotor, Singer Instruments), with four technical replicates per strain placed next to each other in a 2×2 group ...
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bioRxiv - Synthetic Biology 2024Quote: ... Assay cultures were inoculated from the overnight cultures with the Rotor HDA+ screening robot (Singer Instruments, Somerset, UK) into 96-well microtiter plates containing 200 μL of the respective LB medium supplemented with kanamycin and indicated oxacillin concentrations (0 to 100 μg/mL) ...
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bioRxiv - Synthetic Biology 2024Quote: ... The plates were used to inoculate liquid cultures in 384 well format for cryo-preservation and initial screening after overnight incubation with a Rotor HDA+ screening robot (Singer Instruments, Somerset, UK).
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bioRxiv - Synthetic Biology 2024Quote: ... and images were taken with the PhenoBooth plate documentation system (Singer Instruments, Somerset, UK).
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bioRxiv - Synthetic Biology 2024Quote: ... A Rotor HDA+ screening robot (Singer Instruments, Somerset, UK) was afterwards used to transfer droplets onto solid agar plates containing LB kanamycin ...
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bioRxiv - Synthetic Biology 2024Quote: ... 380 single colonies were picked onto 384-well microtiter plates with LB containing kanamycin utilizing the PIXL colony picking robot (Singer Instruments, Somerset, UK). Microtiter plates were incubated overnight on an Multitron HT (Infors AG ...
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bioRxiv - Systems Biology 2024Quote: Colony arrays were grown at 30°C and photographed after 24h and 48h with a colony imager (Phenobooth, Singer Instruments). Subsequent data analysis and visualization were performed using custom scripts in R (R Core Team ...
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bioRxiv - Systems Biology 2024Quote: ... the plates were photographed with a colony imager (Phenobooth, Singer Instruments) to assess strain fitness based on colony size ...
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bioRxiv - Systems Biology 2024Quote: The AID-v1 library was assembled in 1536-colony format on SC(MSG)-Leu+Ade Raf/Gal + hygromycin plates using a pinning robot (Rotor, Singer Instruments). For each ORF ...