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Citations for Takara Bio :
151 - 200 of 3750 citations for ssc mir 369 RT PCR Primer Set since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Developmental Biology 2021Quote: ... Real Time (RT)-PCR was performed (SYBR Premix Ex TaqII, Takara Bio). Reference genes were Actin ...
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bioRxiv - Genomics 2019Quote: ... Reverse transcription was performed with a PrimeScript RT-PCR Kit (TaKaRa, Japan). Quantitative real-time PCR (qRT-PCR ...
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bioRxiv - Microbiology 2021Quote: ... Reverse transcriptase was performed using the PrimeScript™ RT-PCR Kit (Takara). mRNA relative levels of SIGLEC1 were measured by two-step quantitative RT-PCR and normalized to GAPDH mRNA expression using the DDCt method ...
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bioRxiv - Genomics 2019Quote: ... The q-PCR was performed using a PrimeScript RT Reagent Kit (TaKaRa) in a 20-μL reaction volume with 10 μL of 2× SYBR Premix Ex Taq II (TaKaRa) ...
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bioRxiv - Developmental Biology 2020Quote: ... RT-PCR was performed using TaKaRa Ex Taq Hot Start Version (Takara) or PrimeSTAR Max DNA Polymerase (Takara) ...
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bioRxiv - Evolutionary Biology 2021Quote: ... by using One Step TB Green PrimeScript PLUS RT-PCR Kit (Takara) and host RNA-specific primers (Supplementary Table S3) ...
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bioRxiv - Microbiology 2020Quote: ... using the SYBR® PrimeScript™ RT-PCR Kit (Takara, Dalian, China).
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bioRxiv - Microbiology 2020Quote: ... per the manufacturer’s instructions for PrimeScript™ miRNA RT-PCR Kit (Takara). The PCR mixture contained 3 μl cDNA ...
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bioRxiv - Bioengineering 2023Quote: ... Quantitative RT-PCR was performed with TB Green Premix Ex Taq (Takara) using QuantStudio 1 system (Applied Biosystems) ...
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bioRxiv - Cell Biology 2023Quote: ... Quantitative RT-PCR was performed using SYBR Premix EX TaqII (Takara bio) with specific primers listed in Supplementary Table 1 ...
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bioRxiv - Cell Biology 2022Quote: ... C-terminal PA vector was first prepared by insertion of a DNA cassette encoding the PA tag (annealed primer set 28) into the pcDNA3.1(+) vector (digested with XhoI/XbaI) using a DNA Ligation Kit (#6023; TaKaRa, Kusatsu, Japan). The DNA fragment coding for Ms Jaw1 was then amplified by PCR with primer set 29 from pTagGFP2-C Ms Jaw1 (previously described by Kozono et al. ...
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bioRxiv - Synthetic Biology 2024Quote: ... 23S rRNA mutations were introduced to the pLK35 plasmid using the corresponding primer set (Supplementary Table 5) and the In-Fusion Cloning kit (Takara Bio). All sequences were confirmed with full plasmid nanopore sequencing (Plasmidsaurus and Elim Bio).
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bioRxiv - Microbiology 2022Quote: ... a one-step reverse transcription-PCR (RT-PCR) assay was conducted with a PrimeScript kit (Takara, Tokyo, Japan), and Viral genomic terminal sequences were determined using commercial 5’ and 3’ RACE (rapid amplification of cDNA ends ...
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bioRxiv - Microbiology 2022Quote: ... qRT-PCR was performed using the One Step TB Green PrimeScript PLUS RT-PCR Kit (RR096A, TaKaRa, Japan) and a Thermal Cycler Dice Real Time System TP800 (TaKaRa ...
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bioRxiv - Neuroscience 2023Quote: ... Quantitative RT-PCR was conducted on Bio-Rad CFX96 using the SYBR green PCR master mix (TaKaRa, Japan) with the primers listed in Appendix 1—key resources table ...
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bioRxiv - Physiology 2023Quote: ... Reverse transcription (RT)-PCR was performed using the Emerald Amp MAX PCR master mix (Takara Bio, Shiga, Japan). Quantitative RT-PCR was performed using THUNDERBIRD SYBR qPCR MIX (TOYOBO Life Science ...
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bioRxiv - Microbiology 2021Quote: ... RT-qPCR was then performed using gene-specific primers (Table S1) and TB Green Premix Ex Taq (Takara). Levels of gene expression were normalized to that of 16S ribosomal RNA gene (OL4498 and OL4449 ...
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bioRxiv - Microbiology 2020Quote: ... RT-qPCR was then performed using gene-specific primers (Table 2) and TB Green Premix Ex Taq (Takara). Levels of gene expression were normalized to that of 16S rRNA and expression was assessed for each biofilm and each timepoint relative to its planktonic counterpart using the 2−ΔΔCt method (43).
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bioRxiv - Cell Biology 2023Quote: ... put on ice to form RNA/primer complex and mixed with 4.5 µL of RT solution {0.5 µL of SMARTScribe RTase (cat. #639536, Takara), 2 µL of 5x first strand buffer (supplied with RTase) ...
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bioRxiv - Plant Biology 2022Quote: ... The expression analysis of each target gene was performed quantitatively by real-time RT-PCR method after cDNA synthesis using PrimeScript™ RT reagent Kit with gDNA Eraser (TaKaRa) and TB Green® Premix Ex Taq™ II (Tli RNaseH Plus ...
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bioRxiv - Microbiology 2023Quote: ... RT-qPCR was performed in a single-step using the One-Step TB Green PrimeScript RT-PCR Kit II (Takara Bio). The HCoV-OC43 nucleocapsid gene was amplified with the following primers ...
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bioRxiv - Zoology 2024Quote: ... RNA was extracted using Isogen-LS (Nippon Gene) and RT-qPCR was performed using the One-Step PrimeScript RT-PCR Kit (Takara Bio) on a 7500 Real-Time RT-PCR System (Applied Biosystems) ...
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bioRxiv - Synthetic Biology 2024Quote: ... Polymerase chain reaction (PCR) was performed using Primer STAR Max DNA Polymerase (Takara) to amplify the target gene ...
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bioRxiv - Evolutionary Biology 2023Quote: ... Mycoplasma contamination in cell cultures was routinely tested using the PCR mycoplasma detection set (Takara Bio). At approximately 70% confluence ...
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bioRxiv - Plant Biology 2022Quote: ... RT-PCR was conducted using TB Green Premix Ex Taq II (Takara Bio) in a CFX96 Real Time PCR Detection System (Bio-Rad ...
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bioRxiv - Genomics 2020Quote: ... qPCR was performed using One Step SYBR PrimeScript RT-PCR kit (Takara Bio) on a 7900HT real-time system (Applied Biosystems).
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bioRxiv - Immunology 2021Quote: ... utilizing the PrimeScript™ One-Step RT-PCR kit (Takara Bio, Shiga, Japan). PCR products were then separated and visualized by agarose gel electrophoresis ...
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bioRxiv - Genomics 2019Quote: ... Total RNA was reverse transcribed using a PrimeScript RT-PCR kit (TaKaRa Bio), and qRT-PCR was performed using SsoAdvanced Universal SYBR Green Supermix (Bio-Rad Laboratories ...
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bioRxiv - Genetics 2019Quote: ... RT-PCR was accomplished using the SYBR premix Ex Taq kit (Takara, China) on an ABI StepOnePlus Real-Time PCR System (Thermofisher Scientific ...
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bioRxiv - Genomics 2019Quote: ... 12.5μl RNA was used for cDNA synthesis with Advantage RT-PCR kit (Clontech). Detection of pull-down product was performed with qPCR using GoTaq® qPCR Master Mix (Promega ...
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bioRxiv - Genomics 2020Quote: ... Quantitative RT-PCR was performed using SYBR Premix Ex Taq (Takara, Dalian, China) on ABI 7500 system ...
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bioRxiv - Plant Biology 2021Quote: ... and cDNA was synthesized using the one-step PrimeScript RT-PCR Kit (Takara) according to the manufacturer’s instructions ...
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bioRxiv - Plant Biology 2021Quote: ... Then the purified RNA was reversed transcribed using RT-PCR Kit (TaKaRa, Japan) with an oligo dT-adaptor primer in accordance with the manufacturer’s instructions ...
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bioRxiv - Physiology 2021Quote: ... RT-PCR was performed using TaKaRa Ex Taq (Takara Bio Inc., Shiga, Japan) and the following primers ...
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bioRxiv - Developmental Biology 2022Quote: ... Quantitative (q) RT-PCR was performed using SYBR Premix ExTaq 11 (Takara Bio) and detected using the Bio-Rad CFX96 Connect system ...
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bioRxiv - Molecular Biology 2020Quote: ... Quantitative RT-PCR was performed using TB Green Premix Ex Taq II (Takara). Relative gene expression was normalized to ACTB expression ...
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bioRxiv - Genetics 2021Quote: ... Quantitative RT-PCR was performed by using SYBR Premix Ex TaqTM (TaKaRa Bio) on a ABI StepOnePlusTM Real-Time PCR thermocycler (Thermo Fisher Scientific ...
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bioRxiv - Genetics 2021Quote: ... Quantitative RT-PCR was performed by using SYBR Premix Ex TaqTM (TaKaRa Bio) on an ABI StepOnePlusTM Real-Time PCR thermocycler (Thermo Fisher Scientific ...
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bioRxiv - Microbiology 2020Quote: ... RT-PCR was accomplished using the SYBR premix Ex Taq kit (Takara, China) on an ABI StepOnePlus Real-Time PCR System (Thermofisher Scientific ...
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bioRxiv - Cell Biology 2022Quote: ... and a separate master mix was prepared for each primer set using the TB Green® Premix Ex Taq II (TLi RNaseH Plus) reagent kit (RR820A, Takara Bio). The final master mix contained primers at a concentration of 0.4 µM and a 1X concentration of TB Green® Premix Ex Taq II (TLi RNaseH Plus) ...
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bioRxiv - Microbiology 2023Quote: ... vRNA copy numbers of each virus stock were measured via RT-qPCR assay with the One Step TB Green PrimeScript PLUS RT-PCR Kit (Perfect Real Time) (TaKaRa, Cat# RR096A) as described previously [12] ...
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bioRxiv - Cell Biology 2024Quote: cDNA was prepared from 48-hour cultured cells using CellAmp Direct Lysis and RT set (Takara Bio, Shiga, Japan, Cat# 3737S/A) according to the manufacturer’s instructions ...
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bioRxiv - Bioengineering 2021Quote: The target fragments are amplified by PCR using primers (Sangon Biotech, Shanghai, China) and PCR amplification kit (Takara Bio, Japan). The PCR amplifies the DNA fragments including the 1900 bp target ...
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bioRxiv - Cell Biology 2022Quote: All plasmids construction was performed by PCR amplification (CloneAmp HiFi PCR Premix) using appropriate primers followed by Gibson assembly (In-Fusion HD Cloning system, Clontech) into the EcoRI site of pLVX-puro vector (Clontech ...
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bioRxiv - Molecular Biology 2022Quote: ... PCR amplification with Gateway cloning compatible primers was performed directly on Aag2 gDNA using CloneAmp Hifi PCR pre-mix (Takara), without prior amplification in a TOPO TA cloning vector ...
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bioRxiv - Molecular Biology 2023Quote: ... linearized by PCR and using the divergent primers reported in Supplementary Table 3 and CloneAmp™ HiFi PCR Premix (Takara) polymerase ...
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bioRxiv - Biophysics 2024Quote: ... Kir2.1 pathological mutants (C154Y, R312H) were generated by polymerase chain reaction (PCR) with synthetic primers using the CloneAmp HiFi PCR Premix (Takara). Primer sets used for site-directed mutagenesis were the forward sequence ‘5-GTGACTGATGAGTACCCAATTGCAGTGTTTATGGTG –3’ ...
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bioRxiv - Microbiology 2024Quote: ... PCR amplification involved specific primers (Supplementary Table 1) and Ex Taq Polymerase (Takara, Japan) (Choi et al. ...
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bioRxiv - Molecular Biology 2019Quote: ... The PCR amplified EHMT1-Ankyrin and SET products were then cloned into pEGFPC1 vector (6084-1, Clontech) to generate the plasmid constructs ...
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bioRxiv - Neuroscience 2022Quote: ... and were routinely tested for mycoplasma contamination (assayed with PCR Mycoplasma Detection Set, TaKaRa, Cat. no. 6601) and pluripotency status with antibodies against OCT4 (rabbit ...