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Citations for Takara Bio :
451 - 500 of 3750 citations for ssc mir 369 RT PCR Primer Set since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2019Quote: ... We used 0.2 μg of total RNA for reverse-transcription reactions using Advantage RT-for-PCR kit (TakaRa Bio USA). cDNA was used for RT-PCR ...
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bioRxiv - Cell Biology 2020Quote: ... according to the manufacturer’s protocol.Forty ng cDNA was used as templates for RT-PCR using SYBR® Premix Ex Taq kit (TaKaRa,#RR820A) using LightCyclerW 480 (Roche ...
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bioRxiv - Cell Biology 2019Quote: ... RNA with an OD value (A260/A280) of 1.8–2.0 was reversed transcribed to cDNA by using a PrimeScriptTM RT-PCR kit (Takara, Kusatsu, Japan). cDNA was amplified by Takara Taq™ (DR001AM ...
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bioRxiv - Cell Biology 2021Quote: ... Viral RNA was quantified using a One Step TB Green PrimeScript PLUS RT-PCR Kit (Perfect Real Time) (Takara Bio) on a StepOnePlus real-time PCR system (Thermo Fisher Scientific) ...
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bioRxiv - Developmental Biology 2021Quote: ... cDNA synthesis from the total RNA was carried out using the PrimeScriptTM High Fidelity RT-PCR Kit (TaKaRa, Shiga, Japan) using an oligo dT primer ...
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bioRxiv - Cell Biology 2021Quote: ... One microgramme of total RNA was reverse-transcribed using a One Step PrimeScript™ RT-PCR Kit (Takara, Liaoning, China) with a thermocycler ...
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bioRxiv - Biochemistry 2022Quote: A cDNA fragment of Gtsf1L was amplified by RT-PCR from BmN4 total RNAs and cloned into pIZ vector by In-fusion cloning kit (Takara).
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bioRxiv - Molecular Biology 2023Quote: ... The ORF of zebrafish znf598 (ENSDARG00000014945) was amplified by RT-PCR and cloned into pCS2+ via XhoI/XbaI restriction sites using DNA Ligation Kit (TAKARA). To generate znf598 point mutation in ORF (znf598 C13/16A) ...
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bioRxiv - Molecular Biology 2023Quote: ... The ORF of zebrafish rpl36 (ENSDARG00000100588) was amplified by RT-PCR and cloned into pCS2+ via EcoRI/XhoI restriction sites using DNA Ligation Kit (TAKARA). The ORF of zebrafish znf598 (ENSDARG00000014945 ...
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bioRxiv - Molecular Biology 2023Quote: ... Quantitative real-time RT-PCR analysis was performed using TB Green Premix Ex Taq (Clontech, RR420A, Mountain View, CA, USA). The following diluted primers were used ...
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bioRxiv - Plant Biology 2023Quote: ... reverse transcriptase quantitative polymerase chain reaction (RT-qPCR) was employed using the SYBR Green PCR Master Mix (Takara, Dalian, China) in a 48-well plate and analysed using a StepOne Real-Time PCR system (PE Applied Biosystems ...
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bioRxiv - Neuroscience 2024Quote: ... and then the tip of the recording electrode was broken into the nested PCR reagent system (Prime ScriptTm RT reagent Kit with QDNA Eraser, Cat: RR0471, Takara), and a cDNA synthesis kit was used to synthesize cDNA following the manufacturer’s protocol (PrimeScriptm II1st Strand cDNA Synthesis Kit) ...
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bioRxiv - Microbiology 2024Quote: ... The RNA samples were then reverse transcribed employing a One-Step SYBR PrimerScript reverse transcription (RT)-PCR kit (TaKaRa, Japan). Nested PCR conditions were as follows ...
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bioRxiv - Cell Biology 2024Quote: A PCR product containing the Human ORF for DDX3X was obtained directly from RNA using the Primescript High Fidelity RT-PCR kit from Takara. The PCR primers introduced BamHI and NotI sites at the 5’ and 3’ ends respectively ...
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bioRxiv - Developmental Biology 2019Quote: ... RT e5-oligo (Takara), nuclease-free water ...
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bioRxiv - Microbiology 2024Quote: ... using PrimeScript RT (TAKARA) and random hexamers ...
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bioRxiv - Microbiology 2022Quote: ... with HisTALON™ Buffer Set (Clontech Laboratories, 635651) following manufacturer’s instructions ...
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bioRxiv - Cell Biology 2020Quote: ... Synthesised cDNA was used for analysing the expression of specific genes using gene specific primers (as given in supplementary table 1) in presence Taq polymerase master mix (EmaraldAmp GT PCR Master mix, Takara, Japan).
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bioRxiv - Developmental Biology 2021Quote: ... three gene-specific reverse primers were designed and a nested PCR program was used according to the protocol of a genome walking kit (Takara, Japan). The sequences of the primers used for amplification are shown in Table S1 ...
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bioRxiv - Evolutionary Biology 2021Quote: First amplified with primers 27F (5’-AGAGTTTGATCMTGGCTCAG-3’) and 1492R (5’-GGTTACCTTGTTACGACTT-3’) using EmeraldAmp GT PCR Master Mix (TAKARA BIO) to minimize the amplification of non-bacterial taxa under the following cycling conditions ...
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bioRxiv - Cell Biology 2020Quote: ... and digested with NotI and MluI enzymes (restriction sites introduced via the PCR primer sequences) for introduction into similarly-digested pRetro-X-Tight (Takara Bio). The final insertion was confirmed by sequencing.
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bioRxiv - Cell Biology 2021Quote: ... was amplified from HEK293 cDNA by PCR using the primers 5’-TGTAAGCTTTTCGACACCACACCCCACTC-3’ and 5’-AGAGAATTCTCAGGAAAAGCTGTCATCGG-3’ and was inserted into pEGFP-C3 vector (TaKaRa Bio) using EcoRI and HindIII ...
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bioRxiv - Cell Biology 2021Quote: ... The coding sequences of Arfs were amplified by PCR (primers listed in Suppl. Table S1) and inserted into the pQXCIP plasmid (Takara Bio) using the AgeI and BamHI restriction sites ...
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bioRxiv - Molecular Biology 2020Quote: Specific DNA sequences were PCR amplified using gene specific primers and cloned into vectors using PrimeSTAR HS DNA polymerase (#R010A, Takara-bio) and T4 DNA ligase (#M0202S ...
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bioRxiv - Microbiology 2022Quote: ... the coding sequences of DivIVA and its T19A and T19E mutant alleles were PCR amplified using sequence-specific primers (Table 1) and cloned at BamHI-KpnI sites in pDsRed plasmid (Clontech Inc.) yielding pDsD4A and pDsD4AT19A ...
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bioRxiv - Zoology 2020Quote: ... Diluted cDNA was amplified using gene-specific primers (Table 1) and the TB Green real-time PCR master mix (TaKaRa, Japan). RT–PCR was used to verify the accuracy of the RNA-Seq data and detect the mRNA expression of the MYL2 gene in tissues and C2C12 cells at least in triplicate with specific paired primers.
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bioRxiv - Microbiology 2020Quote: ... 1 μl sense and 1 μl anti-sense primers of 100 μM each were mixed with 2 μl 10X Taq polymerase PCR buffer (Takara, Japan) and 16 μl ultra-pure water to a final volume of 20 μl ...
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bioRxiv - Microbiology 2021Quote: ... The genome DNA was used as template for PCR amplification with the barcoded primers and Tks Gflex DNA Polymerase (Takara, USA). The hypervariable V3-V4 regions of bacterial 16S rRNA were amplified using universal primers 343F 5’-TACGGRAGGCAGCAG-3’ and 798R 5’-AGGGTATCTAATCCT-3’ (Nossa et al. ...
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bioRxiv - Microbiology 2021Quote: ... coli codon optimised pbp1 was PCR amplified using primers OPPF20018F/OPPF20018R and cloned into KpnI and SfoI cut pOPINJB by In-Fusion cloning (Takara Bio). The resulting construct ...
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bioRxiv - Microbiology 2021Quote: ... 0.4 µ mol of each primer and 0.7 U of Terra PCR Direct Polymerase (ClonTech Europe, Saint-Germain-en-Laye, France).
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bioRxiv - Genetics 2024Quote: ... oligonucleotide primers were designed to introduce the respective mutant sites using the PCR-based In-Fusion cloning technique (Takara Bio, Japan). The transgenes with human reference wildtype COL4A5 and select patient-derived variants were introduced to the 51C attP landing site on the second chromosome by Rainbow Transgenic Flies (CA ...
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bioRxiv - Cell Biology 2021Quote: ... PrimerScript RT Master Mix and PrimerScript RT Master Mix were purchased from Takara Bio Inc (Shiga ...
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bioRxiv - Plant Biology 2022Quote: ... and then used for RT reactions using the PrimeScript RT Reagent Kit (Takara), with an equimolar mix of random hexamers and polyA primers ...
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bioRxiv - Cell Biology 2023Quote: ... Reverse transcription (RT) reactions were performed using the PrimeScript RT reagent kit (Takara) in conjugation with custom stem-loop primers specific to siRNA guide strands ...
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bioRxiv - Microbiology 2021Quote: ... and Oligo dT Primer (TaKaRa) in a total 20 µl reaction as recommended by the producers ...
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bioRxiv - Immunology 2022Quote: ... Universal primer A mix (CLONTECH) and 5’-GGGRCCARKG GATAGACHGATGG-3’ (designed according to the C segment of mouse IgG antibody heavy chain sequence ...
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bioRxiv - Immunology 2022Quote: ... Universal primer A mix (CLONTECH) and 5’-5’ s-CTTCAGAGGA AGGGTGGAAACAGG-3’ (designed according to the C segment of mouse IgG antibody light chain sequence ...
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bioRxiv - Biochemistry 2023Quote: ... Primers were obtained from Takara Bio Inc ...
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bioRxiv - Immunology 2019Quote: ... Total RNA was then extracted from macrophages using the TRIzol method and converted into cDNA by PrimeScript™ RT-PCR Kit (Takara). The following genes were amplified ...
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bioRxiv - Biochemistry 2019Quote: ... cDNAs were synthesized from the isolated RNA using a cDNA synthesis kit (Advantage RT-PCR kits) from Clontech (Mountain View, CA). Specific primers designed according to human ...
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bioRxiv - Synthetic Biology 2021Quote: ... An aliquot of 1 μg of DNAse treated RNA was used for cDNA synthesis using the PrimeScript™ RT-PCR Kit (Takara) in a final volume of 20 μL according to the manufacturer indications ...
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bioRxiv - Cancer Biology 2021Quote: We quantified mRNA expression levels of IL-10 and chemokines identified in the RNA-Seq analysis using 2-step real-time RT-PCR (Thermal Cycler Dice Real Time System, Takara Bio). The ribosomal protein L13a (RPL13A ...
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bioRxiv - Molecular Biology 2022Quote: ... Real-time RT-PCR analysis was performed using the Thermal Cycler Dice Real-Time System II (Takara Bio Inc., Shiga, Japan) to detect the mRNA expression of GPX4 ...
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bioRxiv - Plant Biology 2021Quote: The expression of DGAT1s in the transgenic Arabidopsis lines was analyzed by quantitative RT–PCR (qPCR) on a Thermal Cycler Dice Read Real-Time System (Takara, Japan) with the SYBR Green Premix EX Taq GC™ (Takara ...
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bioRxiv - Synthetic Biology 2021Quote: ... Aliquots of 1 μg of the treated RNA were used for cDNA synthesis with oligo dT using PrimeScript™ RT-PCR kit (Takara). cDNAs (0.4 μl ...
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bioRxiv - Plant Biology 2021Quote: ... 1 μg of the total RNA from the leaves was reverse-transcribed for 30 min at 42°C in a 20-μl reaction volume using a High Fidelity PrimeScriptTM RT-PCR kit (Takara, Japan) according to the manufacturer’s instructions ...
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ER exit sites in Drosophila display abundant ER-Golgi vesicles and pearled tubes but no megacarriersbioRxiv - Cell Biology 2021Quote: ... the coding sequence of each gene was amplified from whole larva cDNA using PrimeScript RT-PCR Kit (Takara, cat # RR014-A). The resulting products were recombined into pDONR221 and transferred to the pTWGA vector to finally produced the desired plasmids ...
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bioRxiv - Microbiology 2020Quote: ... were used to transcribe poly (A)-tailed miRNAs to generate a cDNA library according to the PrimeScript™ miRNA RT-PCR Kit (Takara) instructions ...
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bioRxiv - Microbiology 2021Quote: ... First the coding region of Candid #1 RdRp was amplified using RNA extracted from P0 or P11 viruses and the PrimeScript II High Fidelity One Step RT-PCR Kit (Takara Bio). Kozak sequence ...
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bioRxiv - Cell Biology 2022Quote: ... NM_001123007) was amplified from 24 hpf embryo RNA using the PrimeScript II High Fidelity One Step RT-PCR kit (Takara, R026A) and the following primers ...