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Citations for Takara Bio :
251 - 300 of 3733 citations for hsa let 7f RT PCR Primer Set since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Developmental Biology 2019Quote: ... Complementary DNA was synthesized with 1μgtotal RNA using a PrimeScript™ RT-PCR Kit (Takara). The real-time PCR with the mixture reagent SYBR Green (Thermo Fisher Scientific ...
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bioRxiv - Cell Biology 2021Quote: ... cDNA was synthesized from 1μg of RNA via the One Step RT-PCR Kit (TaKaRa). Quantitative real time PCR was performed using the iQTM SYBR Green Supermix PCR kit with the iCycler apparatus system (Bio-Rad) ...
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bioRxiv - Plant Biology 2020Quote: ... Real-time quantitative RT-PCR was performed with TB Green Premix Ex Taq™ (TakaRa) using a 7500 real-time PCR system (Applied Biosystems Inc.) ...
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bioRxiv - Microbiology 2020Quote: ... Amplification was performed using a One Step PrimeScript RT-PCR Kit (Takara Bio, Otsu, Japan), and the following real-time PCR conditions were applied ...
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bioRxiv - Plant Biology 2021Quote: ... Youren RT-PCR products were cloned into the pENTR™/D-TOPO™ vector (Takara) and sequenced by Sanger sequencing (Applied Biosystems 3500).
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bioRxiv - Neuroscience 2022Quote: ... For first-strand cDNA synthesis the Takara PrimeScript RT-PCR Kit (Takara Bio, Shiga, Japan) was used with 50 μM Oligo (dT ...
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bioRxiv - Genomics 2020Quote: ... The cDNA first strand synthesis was done using a PrimeScript RT-PCR Kit (Clontech, USA) and quantitative real time PCR was performed following manufacturer’s instructions given in SYBR1 Premix ExTaq (TliRNAse H Plus ...
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bioRxiv - Animal Behavior and Cognition 2020Quote: ... total RNA was reverse transcribed to cDNA using PrimeScript RT-PCR Kit (Takara, Dalian, China). All primers used in this study were recorded in Table 1 ...
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bioRxiv - Cancer Biology 2019Quote: ... The RNA were reverse transcribed using the Advantage® RT for PCR kit (Applied Clontech) and gene expression was analyzed by RT-qPCR with the LighCycler® 480 Real-Time PCR system ...
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bioRxiv - Cell Biology 2021Quote: ... 1.0μg of total RNA was used to prepare cDNA using Titanium RT-PCR kit (TaKaRa). The real-time PCR was performed on the cDNA using mouse TERT and FST primers as described previously (27 ...
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bioRxiv - Microbiology 2022Quote: ... Purified RNA product was immediately used with the One-step PrimeScript RT-PCR Kit (Takara). Primers and probes were obtained from IDT ...
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bioRxiv - Developmental Biology 2022Quote: ... Reverse transcription was performed with the PrimeScript High Fidelity RT-PCR Kit (Takara, Cat. R022A). RT-PCR was run on a 7900HT Fast RT-PCR instrument (Applied Biosystems ...
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bioRxiv - Molecular Biology 2022Quote: ... RT–PCR and agarose gel electrophoresis were performed after RNA extraction with TRIzol (Takara, China). The primers are listed in the Supplementary Table 4.
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bioRxiv - Developmental Biology 2023Quote: ... HpCTCF cDNA was amplified by RT-PCR with TaKaRa LA-taq (TaKaRa Bio Inc., Japan) using the following primers (5’-GTTCGCCATTCATTTGAGTGGATATTTC-3’ and 5’- CTGATCAGGTAAACTCTAAATCAGATTCC- 3’).
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bioRxiv - Genetics 2023Quote: ... according to the steps in the instruction book of SYBR PrimeScript RT-PCR kit (Takara). We used 2-ΔΔc to indicate the relative gene expression level of each sample.
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bioRxiv - Neuroscience 2023Quote: ... Quantitative RT-PCR was performed using Premix Ex Taq II (Takara Bio Inc., Shiga, Japan) on a ViiA 7 Real-Time PCR System (Thermo Fisher Scientific ...
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bioRxiv - Microbiology 2023Quote: ... Purified RNA product was immediately used with the One-step PrimeScript RT-PCR Kit (Takara). Primers and probes were obtained from IDT ...
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bioRxiv - Bioengineering 2023Quote: ... Quantitative RT-PCR was performed using TB Green Premix Ex Taq II (Takara Bio Inc.) in an Applied biosystems Quantstudio 5 ...
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bioRxiv - Developmental Biology 2021Quote: ... which then subjected to PCR using Taq Master Mix (Vazyme, p112-01) for RT-PCR or TB Green Premix EX Taq Ⅱ (Takara, RR820A) for quanti tative PCR ...
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bioRxiv - Microbiology 2023Quote: ... levels were measured by a qRT-PCR assay using the One Step TB Green PrimeScript PLUS RT-PCR Kit (Perfect Real Time) (TaKaRa, Cat# RR096A). The PCR protocol was 42°C for 5 min ...
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bioRxiv - Microbiology 2023Quote: ... The cells were cultured again overnight and subjected to the quantification of mRNA by qRT-PCR using the CellAmp Direct RNA Prep Kit for RT-PCR (Real Time) (3732, Takara Bio Inc.), One Step TB Green PrimeScript PLUS RT-PCR Kit (Perfect Real Time ...
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bioRxiv - Plant Biology 2023Quote: ... The expression of marker genes was detected by qRT-PCR using the One-step TB Green PrimeScriptTM RT-PCR kit II (Takara, Osaka, Japan). All genes were normalized against the level of an actin reference gene (Foo et al. ...
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bioRxiv - Cell Biology 2024Quote: ... The absence of ATG14 transcripts was verified by Reverse Transcription PCR (RT-PCR) on cDNA synthesized from total RNA extracted from the cell samples using Terra™ PCR Direct Polymerase Mix (TAKARA, 639270). Total RNA was isolated using the NucleoSpin RNA II kit (Macherey-Nagel) ...
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bioRxiv - Neuroscience 2021Quote: ... Mutagenesis of pMU2-Camk2b was conducted by inverse PCR with Mighty Cloning Reagent Set (Blunt End) (Takara Bio, Japan) following to the manufacturer’s protocol.
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bioRxiv - Microbiology 2020Quote: ... Cells were originally obtained from the ATCC and routinely tested negative for mycoplasma contamination (PCR Mycoplasma Detection Set, Takara).
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bioRxiv - Microbiology 2021Quote: ... specific primers were designed and PCR reactions were performed using SYBR Green Premix Ex Taq (Takara), according to the manufacturer’s protocol and the primers listed in Table S4 ...
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bioRxiv - Molecular Biology 2022Quote: ... EBIV M/L segment-specific sequences were amplified by PCR including Universal Primer A Mix (Takara), Gene-Specific Primers (GSPs ...
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bioRxiv - Evolutionary Biology 2021Quote: Amplified with V4 16S rRNA primers as above using EmeraldAmp GT PCR Master Mix (TAKARA BIO).
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bioRxiv - Developmental Biology 2023Quote: ... cDNA was amplified with V3d(T)24 and R2SP primers and Terra PCR Direct Polymerase (Clontech) for 16 cycles ...
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bioRxiv - Neuroscience 2023Quote: ... AAV was titrated using quantitative PCR (qPCR) with primers for the ITR sequence (TaKaRa Bio Inc).
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bioRxiv - Immunology 2024Quote: ... Real-Time PCR was performed by using specific primers and SYBR Green ROX Mix (RR420A, Takara). β-actin was used as the internal quantitative control.
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bioRxiv - Genetics 2019Quote: ... A single primer (primer IIA from the Clontech SMARTer kit 5’ AAG CAG TGG TAT CAA CGC AGA GTA C 3’ ...
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bioRxiv - Microbiology 2022Quote: ... A maximum of 500 ng of RNA were reverse transcribed with both oligo dT and random primers using a PrimeScript RT Reagent Kit (Perfect Real Time, Takara Bio Inc.) in a 10 µL reaction ...
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bioRxiv - Plant Biology 2019Quote: ... Two micrograms of total RNA and 200 ng of a random primer were used in reverse transcription (RT) through the cDNA synthesis kit (TaKaRa, Dalian, China) according to the manufacturer’s protocol ...
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bioRxiv - Microbiology 2020Quote: ... RNA was reverse-transcribed into cDNA using random hexamer primers and the PrimeScript RT Reagent Kit with gDNA Eraser (Perfect Real Time; Takara, Dalian, China). For the target genes ...
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bioRxiv - Microbiology 2021Quote: RsmY and RsmZ expression quantification was done by RT-PCR using Mir-X miRNA qRT-PCR TB Green Kit (Takara Bio USA, Inc). For each reaction ...
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bioRxiv - Microbiology 2023Quote: ... The extracted RNAs were subjected to one-step real-time PCR using the One Step PrimeScript™ RT-PCR Kit (Perfect Real Time) (TaKaRa Bio Inc.).
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bioRxiv - Neuroscience 2021Quote: ... Reverse transcription for quantitative PCR (qPCR) analyses was performed with PrimeScript RT Master Mix kit (Takara). qPCR was performed with TransStart Top Green qPCR SuperMix kit (TransGen).
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bioRxiv - Molecular Biology 2020Quote: ... Quantitative real-time RT-PCR analysis was performed using TB Green Premix Ex Taq (Clontech, RR420A) and following primers ...
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bioRxiv - Genomics 2020Quote: ... 500-1000ng of total RNA was reverse transcribed using the Advantage RT-for-PCR Kit (Takara) and qPCR performed in quadruplet using SYBR Green PCR Master Mix (Thermo Fisher Scientific ...
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bioRxiv - Developmental Biology 2019Quote: ... Quantitative real-time RT–PCR was carried out using the SYBR premix Ex Taq kit (TaKaRa). Briefly ...
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bioRxiv - Microbiology 2021Quote: ... Quantitative RT-PCR was performed with SYBR® Premix Ex Taq™ (Takara Bio, Shiga, Japan) in a 7300 Real-Time PCR system (Applied Biosystems ...
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bioRxiv - Microbiology 2022Quote: ... Samples were analyzed using a One-Step SYBR PrimerScript reverse transcription (RT)-PCR kit (TaKaRa, Japan) on Applied Biosystems QuantStudio ...
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bioRxiv - Evolutionary Biology 2021Quote: ... Samples were analyzed using a One-Step SYBR PrimerScript reverse transcription (RT)-PCR kit (TaKaRa, Japan) on an Applied Biosystems QuantStudio ...
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bioRxiv - Developmental Biology 2021Quote: ... PrimeScript RT-PCR and SYBR Premix ExTaq Kits were from Takara (Cat#2641A, Cat#RR420, Japan). BCA protein assay and ECL kits were from Beyotime Institute of Biotechnology (Shanghai ...
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bioRxiv - Microbiology 2019Quote: ... cDNA was synthesized with 100 ng RNA using Advantage RT-PCR Kit (Clontech, Mountain View, USA). Reaction condition for real time PCR was 95°C for 3min (1 cycle) ...
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bioRxiv - Genetics 2021Quote: Isolated RNA from OSF and control tissues was immediately subjected to realtime RT PCR (using Takara PrimescriptTM one step realtime RT PCR kit ...
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bioRxiv - Biochemistry 2021Quote: ... qRT-PCR analysis was performed using cDNA synthesized by the Prime Script RT Reagent Kit (TaKaRa). Each qRT-PCR was conducted in a 20-μL reaction mixture containing 2 μL of diluted template cDNA ...
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bioRxiv - Genetics 2022Quote: ... Reverse transcription for quantitative PCR (qPCR) analyses was performed with PrimeScript RT Master Mix kit (Takara). qPCR was performed with TransStart Top Green qPCR SuperMix kit (TransGen).
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bioRxiv - Cell Biology 2023Quote: ... Lentiviral titration was performed by RT-qPCR using the Lenti-X qRT-PCR Titration Kit (Takara) and the concentration of lentivirus used for this paper was found to be above 1 × 107 copies / mL ...