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Citations for Takara Bio :
501 - 550 of 3733 citations for hsa let 7f RT PCR Primer Set since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Plant Biology 2020Quote: ... After 1 μg of total RNA from leaves was reverse-transcribed using a High Fidelity PrimeScript™ RT-PCR Kit (Takara, Japan), and Real-time qRT-PCR was performed using a Thermal Cycler Dice® Real Time System III T950 (Takara ...
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bioRxiv - Molecular Biology 2019Quote: ... LINC00662 (NR_027301) and ELK4 (NM_001973.4) were detected by One-Step SYBR PrimeScript RT-PCR Kit (Perfect Real Time; Takara Bio, Inc., Kusatsu, Japan). Relative expression values were calculated using the relative quantification (2−△△Ct ...
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bioRxiv - Cancer Biology 2020Quote: ... The ICELL8® 3’ DE Chip was placed on the ICELL8® MSND and loaded 50 µL of RT-PCR solution (Takara Bio ...
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bioRxiv - Plant Biology 2020Quote: The full CDSs of CpPT2 and CpPT3 were amplified from grapefruit flavedo by RT-PCR using TaKaRa Ex Taq polymerase (Takara, Kusatsu, Japan) and the primer pairs for CpPT2 (CpPT2_Fw and CpPT2_Rv ...
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bioRxiv - Neuroscience 2020Quote: ... RT-PCR analysis was performed using the TB Green® Premix Ex Taq™ Tli RNaseH Plus kit (TAKARA, Mountain View, CA). The gene-specific primer sequences were as follows ...
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bioRxiv - Cancer Biology 2021Quote: ... according to instructions provided by the manufacturer and 2μg aliquots were reversed transcribed cDNAs using PrimeScript™ RT-PCR Kit (Takara, Dalian, China). After ...
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bioRxiv - Cell Biology 2022Quote: ... cDNA were amplified from the embryo RNA using PrimeScript™ II High Fidelity One Step RT-polymerase chain reaction (PCR) Kit (Takara, R026A) using the following primers ...
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bioRxiv - Molecular Biology 2022Quote: ... The full-length coding region of chicken CDKN3 (accession number: NM_001252162.1) was amplified by RT-PCR and cloned into the empty vector pCMV-HA (Clontech, San Francisco, CA, USA) to construct the overexpression plasmid pCMV-HA-CDKN3 ...
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bioRxiv - Molecular Biology 2022Quote: ... qPCR was performed using a One-Step SYBR Green Prime ScriptTM RT-PCR Kit II (Perfect Real Time, Clontech, Takara, Kyoto, Japan) with a Thermal Cycler Dice™ system (Takara ...
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bioRxiv - Molecular Biology 2022Quote: ... qPCR was performed using a One-Step SYBR Green Prime ScriptTM RT-PCR Kit II (Perfect Real Time, Clontech, Takara, Kyoto, Japan) with a Thermal Cycler Dice™ system (Takara ...
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bioRxiv - Microbiology 2023Quote: Standards for determining copy number of virus stock were prepared using the PrimeScript II High Fidelity One Step RT-PCR Kit (TaKaRa, Cat# R026A) with IAV (H1N1 ...
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bioRxiv - Microbiology 2024Quote: ... The PCR products were cloned into a PCR linearized pEcgRNA-guide plasmid to make the pEcgRNA-guide-RT plasmid using In-fusion® HD-cloning kit (Takara Bio) following the manufacturer’s instructions.
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bioRxiv - Cell Biology 2021Quote: ... cDNA was amplified using primers P5 and primer PE_miRCat_PCR and TaKaRa LA Taq polymerase (Takara Bio). PCR products were separated on a 2% MetaPhor agarose (Lonza ...
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bioRxiv - Developmental Biology 2020Quote: ... Reverse transcription (RT) was performed using the PrimeScript RT master mix (cat. #RR036A, Takara, Japan). Real-time RT-PCR was performed in a StepOne plus system (ABI StepOne Plus ...
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bioRxiv - Immunology 2020Quote: ... RT was performed on a Bio-Rad CFX machine using the RNA RT Kit (Takara) with a 10μl total reaction volume per well containing 3μl of RNA samples.
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bioRxiv - Developmental Biology 2020Quote: ... coding sequences were amplified by PCR from zebrafish cDNA using specific primers (Table S1) and subcloned into mCherry-C1 vector (Clontech, Mountain View, CA). hVPS4A in pEGFP-C1 was previously described in Elia et ...
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bioRxiv - Biochemistry 2024Quote: ... Sub-library specific primers were then used to amplify 1 ul of cDNA template for 24 cycles using Advantage 2 PCR Mix (Takara Bio, Cat. 639206). Finally ...
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bioRxiv - Cell Biology 2022Quote: ... and oligo(T)18 primers (Takara). Relative transcript amounts were measured by SYBR-green quantitative PCR (qPCR ...
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bioRxiv - Immunology 2019Quote: ... paired with a universal primer (Takara Bio ...
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ARL3 Mediates BBSome Ciliary Turnover by Promoting Its Outward Diffusion through the Transition ZonebioRxiv - Cell Biology 2021Quote: ... and oligo(T)18 primers (Takara). ARL3 cDNA and cDNAs encoding the six BBS proteins BBS1 ...
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bioRxiv - Molecular Biology 2023Quote: ... with random 6 primers (TAKARA BIO). Thereafter ...
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bioRxiv - Neuroscience 2022Quote: ... mRNA 500 ng was used for cDNA synthesis in 10 μl volume according to the manufacturer’s instructions of PrimeScriptTM RT-PCR kit (Takara Bio Inc., Shiga, Japan). Real-time RT-PCR was then performed using the KAPA SYBR FAST qPCR Master Mix (2x ...
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bioRxiv - Microbiology 2020Quote: ... RNA was extracted with a Bacterial RNA Extraction Kit and was immediately reverse-transcribed into cDNA using a PrimeScriptT™ RT-PCR Kit (TAKARA, Daliang, China). The common primer pair GSPilACF/GSPilACR was used to test genomic DNA contamination ...
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bioRxiv - Microbiology 2021Quote: ... for 15 min and then harvested for quantification of INF α mRNA using the One-step SYBR Prime script RT-PCR kit (TaKaRa, Dalian, China) according to the manufacturer’s protocol.Briefly the total RNA was isolated from TNF-α-treated with Trizol reagent (Takara Bio ...
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bioRxiv - Genomics 2022Quote: ... Extracted RNA from each juvenile (as described in Methods S1) was converted to cDNA using PrimeScript RT-PCR Kit (Takara Bio, Shiga, Japan). The efficiency and specificity of the designed primers was tested through PCR using the GoTaq Green Master kit (Promega ...
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bioRxiv - Microbiology 2023Quote: Quantitative RT-PCR (qPCR) for target genes was performed using TB Green®Premix Ex Taq™ II (Tli RNaseH Plus) (Takara, Japan) and using gene-specific primers listed in Table 2 ...
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bioRxiv - Microbiology 2023Quote: ... and real-time RT-PCR was performed by targeting the SARS-CoV-2 RdRp gene as follows: each 20 µl sample consisted of 12.5 µl One Step PrimeScript™ III RT-PCR Kit (Takara Bio, Shiga, Japan), 0.5 µM Sars-CoV-2 CRV forward primer (5’-TCACCTAATTTAGCATGGCCTCT-3’) ...
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bioRxiv - Immunology 2023Quote: ... which was measured using a real-time PCR assay with a SARS-CoV-2 direct detection RT‒qPCR kit (Takara Bio, Siga, Japan). The IC50 was calculated by IC50 Calculator (https://www.aatbio.com/tools/ic50-calculator ...
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bioRxiv - Zoology 2019Quote: ... each set composed of 1.2 μL of 10X buffer (TAKARA), 0.8 μL of 25 mM MgCl2 ...
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bioRxiv - Cell Biology 2023Quote: ... Ligation was set up using T4 DNA ligase (Takara Bio.). proper nucleotide additions were made to the forward primer to maintain the EGFP ORF ...
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bioRxiv - Biochemistry 2023Quote: The CREB Dominant-Negative Vector Set was purchased from Takara Bio ...
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bioRxiv - Molecular Biology 2020Quote: ... Each cDNA was then amplified by Per2AS specific primers (Table S1) together with the Universal Primer (Clontech). The first PCR products were subsequently amplified by the nested Per2AS specific primers with Universal Primer (Clontech) ...
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bioRxiv - Cell Biology 2023Quote: ... MSI2 ORF (NM_138962.2) was amplified by PCR with specific primers and high-fidelity Ex Taq DNA polymerase (Takara Bio USA, Inc., Mountain View, CA) using a cDNA containing human MSI2 obtained from OriGene (Rockville ...
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bioRxiv - Cancer Biology 2021Quote: ... or PrimeScrip RT Master Mix (Takara) (mRNA) ...
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bioRxiv - Immunology 2021Quote: ... For reverse transcription SmartScribe RT (Clontech) and corresponding buffer was used ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... PrimeScript RT Reagent Kit (Takara, RR037A) was used to generate cDNA ...
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bioRxiv - Plant Biology 2022Quote: ... PrimeScript RT Master Mix (Takara Bio) was used for cDNA synthesis and quantitative RT-PCR was performed with PowerUp SYBR Green Master Mix (Thermo Fisher Scientific) ...
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bioRxiv - Microbiology 2023Quote: ... PrimeScript™ RT Reagent Kit (TAKARA) was used to generate cDNA and FastStart Essential DNA Green Master (Roche ...
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bioRxiv - Immunology 2023Quote: Using PrimeScript RT reagent Kit (TaKaRa), 0.5 µg of total RNAs were reverse transcribed into complementary DNA ...
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bioRxiv - Developmental Biology 2023Quote: ... 5× PrimeScript™ RT mix (TaKaRa) was used to acquire cDNA ...
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bioRxiv - Genetics 2023Quote: ... PrimeScript RT Reagent Kit (Takara, RR037) was used for cDNA synthesis ...
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bioRxiv - Neuroscience 2020Quote: ... and a portion (1 ng) of the RNA were subjected to reverse transcription (RT) with a SMARTer™ PCR cDNA Synthesis Kit (Clontech, Mountain View, CA). Then ...
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bioRxiv - Evolutionary Biology 2023Quote: SARS-CoV-2 cDNA was generated from 100 ng of RNA in a RT-PCR reaction buffer containing 4 μL of 5X PrimeScript IV 1st strand cDNA Synthesis Mix (Takara Bio, Kusatsu, Shiga, Japan), 2 μL of 50 μM random hexamer primer ...
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bioRxiv - Microbiology 2022Quote: ... and 4 μM random hexamer primers (Takara Bio ...
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bioRxiv - Microbiology 2020Quote: ... and Oligo dT15 primer (TaKaRa, Shiga, Japan) or Oligo dT-3 sites Adaptor Primer for 3’ RACE (Takara) ...
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bioRxiv - Cancer Biology 2021Quote: ... The primer pairs were synthesized by TaKaRa and displayed in Table EV3.
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bioRxiv - Physiology 2022Quote: ... The primer sequences were obtained from Takara and listed in supplementary Table 1 ...
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bioRxiv - Molecular Biology 2024Quote: ... using Random Primer DNA Labeling Kit (TaKaRa), according to the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2019Quote: ... Plasmids for transfection were prepared using the NucleoSpin buffer set (Clontech) and VitaScientific columns ...
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bioRxiv - Genetics 2024Quote: ... and NucleoSpin® RNA set for NucleoZOL™ (Takara Bio, 740406.50) following the manufactures specifications ...