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Citations for Takara Bio :
101 - 150 of 714 citations for SARS CoV 2 Spike Glycoprotein S2 Sheep Fc Tag HEK293 Horseradish Peroxidase since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2022Quote: ... the CPER products were transfected into HEK293-C34 cells using TransIT-LT1 (Takara, Cat# MIR2300) according to the manufacturer’s protocol ...
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bioRxiv - Microbiology 2022Quote: ... the CPER products were transfected into HEK293-C34 cells using TransIT-LT1 (Takara, Cat# MIR2300) according to the manufacturer’s protocol ...
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bioRxiv - Microbiology 2021Quote: ... the CPER products were transfected into HEK293-C34 cells using TransIT-LT1 (Takara, cat# MIR2300) according to the manufacturer’s protocol ...
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bioRxiv - Bioengineering 2019Quote: ... HEK293T and CHO-K1 cell lines were ordered from American Type Culture Collection (ATCC) and HEK293-tetON cells and CHO-tetON cells were purchased form Clontech (Takara Bio). Synthetic DNA was ordered from Twist Bioscience.
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bioRxiv - Cell Biology 2022Quote: ... Lentiviruses containing guide RNAs targeting GORAB were created in HEK293 Lenti-X cells (Takara 31966021) plated on 10 cm dishes the day before transfection ...
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bioRxiv - Plant Biology 2023Quote: ... and RNA-seq analysis (150 bp PE reads, Novaseq 6000 platform, by Takara Bio; Table S2) as described above.
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bioRxiv - Molecular Biology 2019Quote: ... We inserted a green fluorescent C-terminal tag (EGFP; Clontech) analogous to a procedure used in a previous study [34] (for details ...
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bioRxiv - Cell Biology 2021Quote: ... which encodes the chaperone protein tag (TaKaRa Bio, Shiga, Japan), following the manufacturer protocol ...
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bioRxiv - Molecular Biology 2022Quote: ... downstream of the His tag with In-Fusion HD (TaKaRa). The Gly172Phe substitution was induced by site-directed mutagenesis.
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bioRxiv - Molecular Biology 2022Quote: ... downstream of the His tag with In-Fusion HD (TaKaRa). The His154Gly substitution was induced by site-directed mutagenesis.
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bioRxiv - Biochemistry 2023Quote: ... Cleavage of the His-tag by HRV3C protease (Takara, 7360) followed the manufacturer’s protocol ...
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bioRxiv - Microbiology 2021Quote: ... Complete nucleoprotein and glycoprotein genes were amplified using Takara long amplicon Taq polymerase with GC buffers (RR02AG Takara Bio USA, Mountain View, CA, USA) using the primers indicated in Table 1 after cDNA synthesis using random hexamer primers and Roche AMV reverse transcriptase (10109118001 Roche ...
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bioRxiv - Microbiology 2020Quote: ... The Spike ectodomain was purified by immobilised metal affinity chromatography using Talon resin (Takara Bio) charged with cobalt followed by size exclusion chromatography using HiLoad 16/60 Superdex 200 column in 150 mM NaCl ...
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bioRxiv - Microbiology 2020Quote: ... The Spike ectodomain was purified by immobilized metal affinity chromatography using Talon resin (Takara Bio) charged with cobalt followed by size exclusion chromatography using HiLoad 16/60 Superdex 200 column in 150 mM NaCl ...
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bioRxiv - Bioengineering 2023Quote: HEK293 cells (JRCB Cell Bank, Osaka, Japan) and AAVpro 293T cells (Takara Bio Inc., Shiga, Japan) were cultured in Dulbecco’s modified Eagle Medium (Sigma Aldrich ...
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bioRxiv - Cell Biology 2022Quote: ... E.coli competent cells transformed with pTf16 chaperone protein tag (TaKaRa Bio) per the manufacturer’s protocol.
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bioRxiv - Molecular Biology 2020Quote: ... The supernatant was incubated with TALON His-tag purification resin (TaKaRa) or glutathione agarose beads (Sigma-Aldrich ...
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bioRxiv - Microbiology 2023Quote: ... the CPER products were transfected into HEK293-hACE2/hTMPRSS2 cells by using TransIT-LT1 (Takara, Cat# MIR2305). At one day post-transfection ...
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bioRxiv - Cell Biology 2021Quote: ... listed in table S2) and inserted the 2xFlag_2xStrep_MST2 constructs (wild type or del_EDG) in the inducible vector pLVX (Clontech, #631847).
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bioRxiv - Plant Biology 2022Quote: ... RT-PCR products of stage 2 ray florets using c25599_g2_i1 Fill-F and c25599_g2_i1 Fill-R primers (Table S2) were digested with SacI (Takara Bio Inc). Reaction mixture was consisted of 2.5 µL of PCR product ...
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bioRxiv - Microbiology 2020Quote: ... Purification tags were removed by treating recombinant proteins with HRV3C protease (TaKaRa) and cOmplete™ His-tag Purification Resin (Roche) ...
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bioRxiv - Bioengineering 2019Quote: Sequencing libraries were prepared using the ThruPLEX Tag-seq Kit (Takara Bio). 10 μL of samples from NanoDeep assays (performed on SPR surfaces or cells ...
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bioRxiv - Cell Biology 2020Quote: ... HEK293 cells were transfected with pX330 vector together with an EGFP-containing plasmid (pEGFP-N1; Clontech Laboratories, Inc). Single cells were sorted using a FACS Vantage SE machine and the knockouts confirmed by sequencing ...
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bioRxiv - Neuroscience 2021Quote: ... The transfection efficiency of HEK293 cells was evaluated by transfecting cells with EGFP-N1 (Clontech; Mountain View, CA) in parallel reactions ...
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bioRxiv - Neuroscience 2022Quote: All viruses were made using the 2nd generation lentiviral packaging systems in Lenti-X HEK293 FTT cells (Takara). Lenti-X cells were passaged maximum 3 times before being used for virus production in HEK media (High glucose DMEM with 4 mM GlutaMAX ...
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bioRxiv - Microbiology 2020Quote: ... the His-tagged spike protein produced in the culture supernatants was purified with a Talon resin (Clontech).
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bioRxiv - Developmental Biology 2024Quote: ... containing 10% tetracycline-free fetal calf serum (FCS) (TaKaRa Bio, 631106), 2 mM L-Glutamine (Thermo Fisher Scientific ...
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bioRxiv - Microbiology 2022Quote: ... The recombinant adenovirus was prepared in HEK293 cells and purified with an Adeno-X Virus Purification kit (Takara Bio). The purified virus titer was determined using an Adeno-X Rapid Titer kit (Takara Bio) ...
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bioRxiv - Neuroscience 2023Quote: ... Plasmid DNA used to generate lentiviral particles were transfected into HEK293 cells using LentiX single-shot VSV-G (Takara) following manufacturer’s instructions ...
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bioRxiv - Neuroscience 2024Quote: ... HEK293 cells were first incubated with ARIAD ligand at a concentration of 1 µM (AL; D/D Solubilizer; Takara) for the indicated times ...
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bioRxiv - Microbiology 2021Quote: ... for expression with a C-terminal GFP tag or into pEGFP-C2 (Clontech) for expression with an N-terminal GFP tag and a codon-optimised synthetic gene (GeneArt ...
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bioRxiv - Cell Biology 2019Quote: FLAG-tag-hFAIM-S expression vector was constructed using pCMV-(DYKDDDDK)-C (Takara) (cloned into EcoRⅠ and KpnⅠ sites) ...
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bioRxiv - Microbiology 2023Quote: ... templates with primers encoding a His-tag and NcoI/NotI cut sites (Takara), cloned into pET22b ...
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bioRxiv - Plant Biology 2020Quote: ... Signal peptide– truncated cDNA fragments of AVRs were amplified by PCR by using primer set (Table S2) and inserted into EcoRI and BamHI sites of pGADT7 (prey) or pGBKT7 (bait) vectors (Clontech). sHMA cDNAs were synthesized from total RNAs of rice leaves (cultivar Sasanishiki ...
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bioRxiv - Genomics 2020Quote: ... A relative real-time quantitative PCR was then carried out for all the genes studied (Table S2) against SCR1 using SYBR Green Premix Ex Taq (Takara) in a Light Cycler 480 II (Roche).
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bioRxiv - Microbiology 2023Quote: ... Genes or gene fragments were PCR amplified with primers (S2 Table) to allow for In-Fusion gene cloning (Takara Bio) according to the manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2024Quote: ... and used with the mixture of specially designed primers (SWITCH2 and RT_universal_primer, Supplementary Table S2) for cDNA synthesis (SMARTScribe Reverse Transcriptase, TaKaRa, Kusatsu, Japan).
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bioRxiv - Microbiology 2022Quote: ... 10 µg of Spike expressor and 2.5 µg of a green fluorescent protein (GFP) expressor (pIRES2-eGFP; Clontech) was transfected into 2 × 106 HEK 293T cells ...
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bioRxiv - Neuroscience 2020Quote: We transiently co-transfected cDNA constructs of GluN1 and GluN2A into mammalian human embryonic kidney 293 (HEK293) with a separate pEGFP-Cl vector (Clontech) at a ratio of 4.5:4.5:1 (GluN1/GluN2A/EGFP ...
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bioRxiv - Plant Biology 2022Quote: ... The PCR reaction was performed with a Biorad CFX384TM Real Time System PCR machine and forward and reverse gene-specific primers (0.5 μM, Table S2) using the SYBR® Premix Ex Taq™ (Tli RNaseH Plus) from Takara Bio ...
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bioRxiv - Molecular Biology 2023Quote: ... The single isoform of DCR1 was amplified by RT-PCR from mRNA isolated from S2 cells and reverse transcribed using the Oligo(dT)-primed RNA to cDNA EcoDry premix (Takara Bio). The CDS for AGO1 and GW182 were PCR amplified from pAFW-Ago1 (Addgene #50553 ...
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bioRxiv - Microbiology 2022Quote: Amplified spike sequence was first gel-purified using NucleoSpin Gel and PCR Clean-up kit (Takara, Cat. No. 740609.5) and then further purified using Ampure XP beads (Beckman Coulter ...
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bioRxiv - Microbiology 2020Quote: ... with an N-terminal GFP tag using the In-Fusion HD Cloning kit (Takara).
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bioRxiv - Microbiology 2020Quote: ... The cDNA fused to a C-terminal HA tag was subcloned into pQXCIH (Clontech) in between the NotI and PacI sites to obtain the pQXCIH-TMPRRS2-HA vector.
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bioRxiv - Neuroscience 2020Quote: ... which was generating by replacing a V5 tag with an eGFP or DsRed (Clontech) tag from pcDNA3.1-nV5-DEST vector (Invitrogen) ...
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bioRxiv - Biochemistry 2019Quote: ... The DNA libraries were prepared using a SMARTer ThruPLEX Tag-seq Kit (Takara Bio), and the samples were sequenced on an Illumina HiSeq1500 system.
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bioRxiv - Biochemistry 2023Quote: ... CBS proteins with a permanent C-terminal His-tag were purified using TALON (Clontech) resin followed by anion exchange using a Hitrap Q column (Cytiva) ...
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bioRxiv - Microbiology 2022Quote: ... CoV-229E sequences were obtained as gBlocks from IDT and subcloned into these plasmids using InFusion cloning (Takara).
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bioRxiv - Plant Biology 2020Quote: ... The genetic regions containing cmaA and surrounding regions were amplified using PCR primer sets (S2 Table) that were designed based on the Pcal ES4326 registered sequence with Prime Star HS DNA polymerase (TaKaRa, Otsu, Japan), then dA was added to the 3’ end of the PCR product with 10 x A-attachment mix (TOYOBO ...
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bioRxiv - Neuroscience 2022Quote: AAVs were packaged via calcium phosphate transfection of HEK293 cells as detailed in (McClure et al 2011) and purified using an AAVpro purification kit (Takara Bio).