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Citations for Takara Bio :
201 - 250 of 918 citations for Recombinant Human Fc Fragment Of LgG Receptor Transporter Alpha since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Synthetic Biology 2022Quote: Each DNA fragments were amplified using PrimeSTAR DNA polymerase (TaKaRa, Shiga, Japan) and subcloned into vectors using T4 DNA ligase (TakaRa ...
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bioRxiv - Neuroscience 2022Quote: ... The fragments were then assembled using In-Fusion HD cloning kit (Clontech) to obtain the desired chimeric OR ...
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bioRxiv - Cell Biology 2023Quote: ... The cDNA fragments were amplified using PrimeSTAR HS DNA polymerase (TAKARA, Japan), and plasmid recombination was performed using NEBuilder HiFi DNA assembly (New England Biolabs) ...
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bioRxiv - Immunology 2024Quote: ... The amplified DNA fragments were ligated into T vector pMD19 (#3271, Takara), and whose nucleotide sequences in each plasmid clones were determined.
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bioRxiv - Immunology 2023Quote: T cell receptors were generated by custom synthesis (Twist Bioscience) in a 3rd generation pLVX-EF1α-IRES-Puro lentiviral vector (Takara/Clontech). Following the TransIT-Virusgen Transfection (Mirus ...
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bioRxiv - Neuroscience 2019Quote: ... and human WT hP-NPO cDNA was amplified from human brain cDNA library (TaKaRa, Cat #637242) with primers ...
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bioRxiv - Cell Biology 2021Quote: ... The nucleotide sequence encoding for human CyPD was obtained from a Human cDNA placenta library (Clontech) by PCR ...
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bioRxiv - Microbiology 2020Quote: Human embryonic kidney 293T (Clontech, 632180), human lung carcinoma A549 (ATCC ...
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bioRxiv - Genomics 2021Quote: Hap1 cells were cultured in Iscove’s Modified Dulbecco’s Medium (IMDM) supplemented with 10% FCS (Clontech), 1% Penicillin/Streptomycin (Invitrogen ...
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bioRxiv - Microbiology 2020Quote: ... Purification of recombinant Ply was performed using Talon resin (#PT1320-1, Clontech Laboratories Inc.) affinity chromatography from freshly transformed BL21/DE3 E ...
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bioRxiv - Biochemistry 2020Quote: Standard recombinant DNA techniques and In-Fusion® HD Cloning (Takara Bio USA, Inc.) were used for plasmids construction in this study and were verified by DNA sequencing ...
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bioRxiv - Cancer Biology 2023Quote: ... Recombinant lentivirus titer was measured using Lenti-XTM GoStixTM Plus (ClonTech, catalog number: 631280). E0771 and RM-1 cells were transduced with lentiviral vectors carrying the luciferase expression cassette with TransDux MAXTM (System Bioscience ...
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bioRxiv - Molecular Biology 2023Quote: Recombinant AAV9 vectors were generated by co-transfection of AAVpro 293T cells (Takara Bio) with three plasmids ...
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bioRxiv - Microbiology 2023Quote: ... These recombinant plasmids were transformed into Escherichia coli BL21 (Takara Bio Co Ltd, Japan) and the transformed cells were cultured at 37°C until the OD600 reached at 0.6–0.8 ...
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bioRxiv - Bioengineering 2021Quote: ... Human umbilical vein endothelial cells (HUVECs) and human aortic smooth muscle cells (HAoSMCs) were purchased from TAKARA BIO (Tokyo ...
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bioRxiv - Pharmacology and Toxicology 2022Quote: Site-directed mutagenesis in GC-A and GC-B and construction of chimeric receptors were performed through use of the In-Fusion HD plus cloning kit (Takara Bio Inc.). The primers used are listed in Supplementary Table 1 ...
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bioRxiv - Molecular Biology 2022Quote: ... The amplified fragments required to generate the chimeric receptor were combined with a linearized pCS2+ backbone and annealed using an In-Fusion kit (Takara Bio, 638947). The resulting plasmids were transformed into Top10 chemically competent cells ...
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bioRxiv - Developmental Biology 2020Quote: ... Fragments were then diluted and ligated with T4 DNA ligase (Takara, Lot # 1211707) at 16°C overnight ...
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bioRxiv - Biochemistry 2019Quote: ... CoAA and hnRNP M fragments were inserted in-frame into pM vector (Clontech) containing Gal4 DNA-binding domain to produce Gal4-fusion proteins ...
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bioRxiv - Cell Biology 2019Quote: ... The fragments were inserted into vectors peGFP-N2 or pmCherry-N2 (originally Clontech) designed for expression of proteins tagged with eGFP and mCherry at the C-terminus by standard methods of molecular cloning ...
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bioRxiv - Genetics 2019Quote: ... the PCR fragment was cloned into a vector using an Infusion kit (Takara), and the linear construct was amplified with T7/T3 primer set from the plasmid template ...
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bioRxiv - Microbiology 2021Quote: ... fragments were PCR amplified from the plasmids using PrimeSTAR GXL Polymerase (Takara, Japan) and Nhel_IDT_F and EcoRI_ITD_R primers (Supplementary table 7) ...
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bioRxiv - Microbiology 2021Quote: ... Allele fragments were generated using the high-fidelity PrimeSTAR Max polymerase (Takara Bio), assembled into strain-specific pEXG2 plasmids in E ...
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bioRxiv - Molecular Biology 2022Quote: ... These three fragments were ligated using In-fusion (Takara Bio Inc, Shiga, Japan), and the resultant plasmid was named psfRps29_WT.
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bioRxiv - Molecular Biology 2022Quote: ... DNA fragments containing eIF4A iso4 were PCR-amplified and inserted into pColdI (TaKaRa) downstream of the His tag with In-Fusion HD (TaKaRa) ...
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bioRxiv - Genomics 2019Quote: ... The IRES2 fragment was PCR amplified from the pTRE3G-IRES (Cat. # 631174, Clontech) using the primer pairs
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bioRxiv - Genomics 2019Quote: Both fragments were then assembled together with a pTRE3G-IRES (Cat. # 631174, Clontech) backbone ...
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bioRxiv - Plant Biology 2019Quote: ... The amplified ORF fragment was then cloned into the pGBKT7 vector (Takara Bio) to generate the pGBKT7-Pha13 and pGBKT7-Pha21 constructs ...
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bioRxiv - Plant Biology 2021Quote: ... Gene fragments were amplified using PrimeStar HS DNA polymerase (Takara Bio, Kusatsu, Japan) and cloned into the vector using In-Fusion HD Cloning Kit (Takara Bio) ...
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bioRxiv - Molecular Biology 2021Quote: ... the amplified fragments were inserted into the pMD™ 19-T Vector (Takara) and re-sequenced ...
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bioRxiv - Synthetic Biology 2020Quote: ... Polymerase chain reaction (PCR) fragments were generated using PrimeSTAR Max DNA Polymerase (TaKaRa). The method for plasmid construction is described below.
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bioRxiv - Physiology 2019Quote: ... The purified DNA fragments were ligated into the pET-32a (+) plasmid (TaKaRa, Japan) and transformed into competent Escherichia coli DH5α cells ...
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bioRxiv - Cell Biology 2021Quote: ... as two overlapping fragments using high-fidelity Takara LA Taq (Takara, Cat # RR002M). Primer details can be found in Supplementary Table 2 ...
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Phospholipid flippases and Sfk1 are essential for the retention of ergosterol in the plasma membranebioRxiv - Cell Biology 2020Quote: ... The GFP and D4H fragments were inserted into pColdI (Takara bio, Shiga, Japan) to construct pColdI-GFP-D4H ...
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bioRxiv - Molecular Biology 2022Quote: ... mEGFP and 3’UTR fragments were amplified by PrimeSTAR Max DNA polymerase (Takara). The replication origin and selection marker were derived from a mammalian expression vector ...
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bioRxiv - Plant Biology 2022Quote: ... The resultant NOS terminator fragment and pENTR_gMpTIR1 were digested with NotI (Takara Bio) and then ligated to generate pENTR_NosT-gMpTIR1 ...
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bioRxiv - Cell Biology 2023Quote: ... delCMV- mCherry was generated by ligating the larger fragment from pmCherry-N1 (Clontech) with the smaller fragment from delCMV-mCherry-actin (Schulze et al. ...
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bioRxiv - Developmental Biology 2023Quote: ... Plasmids were assembled from linear fragments using In-Fusion HD (Takara Bio, 638910) and amplified in Stellar chemically competent E ...
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bioRxiv - Developmental Biology 2024Quote: ... These PCR fragments were fused with the In-Fusion HD Cloning kit (Clontech). The region encoding XY linker43 was deleted from PLCβ1/2/3 cDNA by inverse PCR to create a constitutively active form ...
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bioRxiv - Microbiology 2024Quote: ... the different fragments were amplified with the PrimeSTAR® HS polymerase (Takara Bio) using specific primers flanked by restriction sites ...
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bioRxiv - Neuroscience 2019Quote: ... test genes (vascular connexins) in human vessels were normalized relative to human whole heart (RNA purchased from Clontech) and in mouse ...
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bioRxiv - Neuroscience 2020Quote: ... cDNA of Human Phosphodiesterase type 10 (hPDE10) was amplified by conventional nested PCR using human brain cDNA (Clontech). cDNAs template encoding wild-type and D674A mutant form of hPDE10 catalytic domain (CD ...
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bioRxiv - Developmental Biology 2024Quote: ... Human full-coding KATNA1 cDNA was isolated from a human fetal brain Marathon-ready cDNA collection (Clontech, Invitrogen) with the following forward and reverse primers ...
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bioRxiv - Developmental Biology 2021Quote: ... Single embryos were collected in 2 µl RNase free water with Recombinant RNase inhibitor (TAKARA) and ruptured with an RNase free needle ...
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bioRxiv - Microbiology 2021Quote: ... The recombinant protein was purified using the His60 Ni gravity column purification kit (Takara Bio) according to the manual instruction ...
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bioRxiv - Cell Biology 2019Quote: ... Recombinant proteins of interest were bound to TALON metal affinity beads (Clontech, Mountain View, CA) and eluted with imidazole (gradient 10–200 mM ...
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bioRxiv - Neuroscience 2022Quote: ... with RNAse-free water with 0.4 U/μL Recombinant RNase Inhibitor (Takara Clontech, Ref. 2313A) and a fresh dilution of 1 in 300,000 was prepared before the first strand synthesis.
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bioRxiv - Neuroscience 2022Quote: ... with RNAse-free water with 0.4 U/μL Recombinant RNase Inhibitor (Takara Clontech, Ref. 2313A) and a fresh dilution of 1 in 300,000 was prepared before the first strand synthesis.
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bioRxiv - Plant Biology 2023Quote: ... Recombinant proteins were purified from Escherichia coli BL21 (DE3) with TALON Metal Affinity Resin (Takara), Pierce Glutathione Agarose (Thermo scientific) ...
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bioRxiv - Neuroscience 2024Quote: ... and resuspended in PBS with 0.3%BSA and 1% recombinant RNase inhibitor (RRI, Takara Bio). Next ...