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Citations for Takara Bio :
151 - 200 of 918 citations for Recombinant Human Fc Fragment Of LgG Receptor Transporter Alpha since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Physiology 2019Quote: Human TMEM206 was cloned from cDNA obtained from a human brain cDNA library (Clontech) using the following primers:
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bioRxiv - Developmental Biology 2020Quote: ... Laminin-511 E8 fragment (Takara Bio, #T303, 0.05 – 2 µg/ml) and Laminin-521 (Biolaminin ...
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bioRxiv - Molecular Biology 2020Quote: ... These fragments were then cloned into pEGFP-N1 vector (Clontech, USA) between XhoI and EcoRI sites to produce the following recombinant vectors ...
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bioRxiv - Molecular Biology 2022Quote: ... followed by ligating these fragments using an In-Fusion system (TAKARA). Hevin-N11 fragment was inserted into pCR-blunt using In-Fusion system ...
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bioRxiv - Immunology 2020Quote: ... The amplified fragment was cloned into MCS A of pIRES (Clontech). The reporter gene ECFP was taken from pECFP-N1 (Clontech ...
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bioRxiv - Cell Biology 2022Quote: ... The three fragments were fused using the in-fusion enzyme (Takara) into a pRetroX-TetOne-Puro (Takara ...
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bioRxiv - Microbiology 2019Quote: ... The resulting fragment was digested with NotI (TaKaRa Bio Inc., Japan) and subsequently cloned into the NotI site of pKLC21 to generate PICImid pKLC31.
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bioRxiv - Bioengineering 2020Quote: pAID plasmid was generated by assembling PCR fragments by Infusion(Takara) based clonging from pAID (Addgene ...
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bioRxiv - Molecular Biology 2021Quote: ... Fragments were combined into a plasmid by In-Fusion Cloning (Takara).
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bioRxiv - Microbiology 2021Quote: ... the fragments were restricted using XhoI and NotI (Takara, Shiga, Japan) for the first fragment and NotI and SalI for the second fragment ...
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bioRxiv - Cell Biology 2022Quote: ... the fragments were amplified with PrimeSTAR MAX (Takara Bio, Japan, R045) with the appropriate primer pairs having overlapping sequence ...
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bioRxiv - Neuroscience 2022Quote: ... DNA fragments were amplified using GXL Prime Star DNA polymerase (Takara) using cDNA libraries as templates ...
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bioRxiv - Cancer Biology 2023Quote: ... the fragments were subcloned into the pLVX-puro vector (Takara, #632164) with Ha- or Flag-tag ...
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bioRxiv - Microbiology 2023Quote: ... Fragments were joined via crossover PCR using PrimeStar Max (Takara Bio) per manufacturer instruction ...
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bioRxiv - Bioengineering 2023Quote: ... PCR fragments were amplified with ExTaq DNA polymerase (Takara Bio Inc.). Purified PCR products were denatured and re-annealed using a thermal cycler ...
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bioRxiv - Developmental Biology 2023Quote: DNA fragments were amplified by PCR with exTaq-HS (Takara Bio) and Phusion HF (New England Biolabs ...
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bioRxiv - Biochemistry 2019Quote: ... Cells were transiently transfected either with GluK3EM or co-tansfected with Wild type/mutant receptors along with GFP expressing plasmid (2 µg/dish) using Xfect reagent (Clontech). Currents were recorded from medium sized cells expressing a moderate level of fluorescence from either the fused EGFP in case of GluK3EM or co-expressed EGFP and having a capacitance of ∼5-6 pF at 48-60 hours post transfection ...
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bioRxiv - Immunology 2023Quote: T cell receptors were generated by custom synthesis (Twist Bioscience) in a 3rd generation pLVX-EF1α-IRES-Puro lentiviral vector (Takara/Clontech). Following the TransIT-Virusgen Transfection (Mirus ...
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bioRxiv - Cell Biology 2021Quote: The cDNA encoding human BORCS6 was cloned from human lung total RNA (Takara Bio, Japan) and inserted into pCR4-TOPO (Invitrogen ...
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bioRxiv - Cell Biology 2023Quote: Human MPS1 and NSL1 were amplified from Human testis cDNA (Marathon cDNA; Takara Bio Inc.) using Pfu polymerase (Promega) ...
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bioRxiv - Plant Biology 2020Quote: ... The recombinant plasmids were transformed into Stellar™ Competent Cells (Clontech, Catalog # 636763), and positive colonies were selected on LB plates containing spectinomycin (100ug/mL ...
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bioRxiv - Microbiology 2022Quote: ... The thrombin-digested recombinant NA was incubated with TALON metal affinity resin (Takara) for 2 h ...
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bioRxiv - Developmental Biology 2021Quote: ... and homogenization (NIM2 buffer supplemented with Recombinant RNase Inhibitor (0.4 U/μL, Takara), SUPERase in (0.2 U/μL ...
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bioRxiv - Plant Biology 2021Quote: ... The recombinant plasmid was transformed into Stellar™ Competent Cells (Clontech, Catalog # 636763) according to the manufacturer’s protocol and positive colonies were selected on LB plates containing kanamycin (50 μg/mL).
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bioRxiv - Pathology 2020Quote: ... followed by treatment with 10 U of recombinant DNase I (Cat.#2270A, TaKaRa) to remove residual DNA ...
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bioRxiv - Biochemistry 2021Quote: ... The recombinant proteins were purified with His60 Ni Gravity Column Purification Kit (Clontech) following a manufacturer’s protocol ...
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bioRxiv - Cell Biology 2022Quote: ... the MEFs were transduced using a replication defective recombinant retroviral expression system (Clontech) with either wild-type (Parl WT ...
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bioRxiv - Molecular Biology 2022Quote: ... supplemented with 0.1 U/μL of recombinant RNase inhibitor (wash buffer-RIn, Takara). Mechanical cell lysis was achieved by mixing each sample with 0.1 mm glass beads and grinding in a Retsch mixer mill at a frequency of 30/s for 5 min ...
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bioRxiv - Microbiology 2022Quote: ... The recombinant adenovirus was produced using an Adenovirus Dual Expression Kit (Takara Bio) in accordance with the manufacturer’s protocol ...
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bioRxiv - Microbiology 2023Quote: ... Extracted RNA (20µg) was treated with recombinant DNase I (RNAse free) (Takara, Japan) and then cleaned-up using RNeasy spin column (Qiagen ...
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bioRxiv - Plant Biology 2023Quote: ... The extracted RNA was treated with Recombinant DNase I (2270A, Takara, Shiga, Japan), and 2 ng equivalent was synthesized using Super Script 3 Supermix (18080400 ...
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bioRxiv - Immunology 2023Quote: ... tubes with 1:1 FBS:PBS supplemented with recombinant RNase inhibitor (1:100, Takara). The live singlet gated CD3+ T cells were further gated as per the gating strategy shown in additional file 1 ...
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bioRxiv - Neuroscience 2021Quote: ... The amplified fragment was ligated using the In-Fusion cloning kit (Takara) into the pGL4.13[luc2/SV40] (E6681 ...
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bioRxiv - Genomics 2020Quote: ... After both ends of the plasmids were blunted using Klenow fragments (Takara), they were ligated by DNA ligase (Takara).
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bioRxiv - Microbiology 2019Quote: ... The fragment was first cloned into the pLacZ-Basic vector (Clontech, Japan) at KpnI/Xho sites ...
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bioRxiv - Developmental Biology 2019Quote: ... The TTN cDNA fragment was cloned into pEGFP-C1 (Clontech, CA, USA), while the MURF1 cDNA fragment was cloned into myc-tagged pCMV-Tag3 (Stratagene ...
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bioRxiv - Microbiology 2019Quote: ... The resulting fragment was cell-free cloned into pNI-His (Takara Bio) for expression in B ...
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bioRxiv - Molecular Biology 2020Quote: ... The amplified fragments were inserted into pMD19-T vectors (Takara; Tokyo, Japan), and 10-15 clones for each gene were selected for sequencing ...
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bioRxiv - Plant Biology 2020Quote: ... and the resulting fragments were ligated using a DNA Ligation kit (Takara). The cDNAs of NPH3SA and NPH3SE within the pENTR vectors were transferred to both pH35GS and pH35YG binary vectors (Yamaguchi et al. ...
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bioRxiv - Microbiology 2021Quote: ... the two fragments were generated using CloneAmp HiFi PCR Premix (Takara Bio) by amplifying the TurboGFP::Luc open reading frame from the Vector Gal4-TurboGFP-Luc using the primers GA_TurboGFP::Luc_pLentiBlast-StrepOneOv_For (acaaaaaagcaggctccaccATGGAGAGCGACGAGAGC ...
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bioRxiv - Biochemistry 2020Quote: ... Fragments were cloned into mammalian expression vectors based on pEGFP-C1 (Clontech), bearing an N-terminal EGFP tag or modified to contain either HA or HA-Flag tags (provided by Elisa Izaurralde ...
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bioRxiv - Genetics 2019Quote: ... the mCherry and pyrG fragments were added by In-Fusion cloning (Takara) using primers with appropriate overlapping 5’-tails (Supplementary Table 2) ...
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bioRxiv - Genetics 2019Quote: ... The PCR amplified DNA fragment was assembled in In-Fusion reaction (Takara® In-Fusion® HD Eco-Dry™ Cloning Plus Cat#638915 ...
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bioRxiv - Molecular Biology 2020Quote: ... PCR amplification for cloning gene fragments was done using PrimeSTAR Max (TaKaRa). Cloning for assembling was done using Quick ligation kit (NEB) ...
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bioRxiv - Microbiology 2021Quote: ... then the fragments were circularized using In-Fusion HD (Takara Bio Inc.). To generate pAGM4723-ChCEC3KO ...
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bioRxiv - Molecular Biology 2021Quote: ... the target DNA fragments were amplified by PCR (TaKaRa EpiTaq HS; TAKARA). The PCR fragment was purified by precipitation with PEG 8000 ...
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bioRxiv - Molecular Biology 2021Quote: ... the target DNA fragments were amplified by PCR (TaKaRa EpiTaq HS; TAKARA). The PCR fragment was purified by precipitation with PEG 8000 ...
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bioRxiv - Biochemistry 2021Quote: ... Ligation of DNA fragments was performed using In FusionTM cloning (Takara, Japan). Final constructs were verified by Sanger sequencing (EUROFINS ...
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bioRxiv - Molecular Biology 2022Quote: ... Amplification of target fragments was performed by PrimeSTAR Max DNA Polymerase (Takara).
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bioRxiv - Cell Biology 2022Quote: ... Linear DNA fragments were produced by PCR using HiFi polymerase (Takara Bio), followed by treatment with DpnI to remove template DNA ...