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Citations for Takara Bio :
151 - 200 of 5049 citations for Rat High Mobility Group Protein B1 HMGB1 ELISA Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2020Quote: ... The PCRs were performed with a high-fidelity DNA polymerase (PrimeStarMax, Takara) (see section Detailed protocol for chimeric PCR for mutation in A ...
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bioRxiv - Synthetic Biology 2021Quote: PCR was performed using the high-fidelity polymerase PrimeSTAR GXL DNA (Clontech). Golden Gate assembly was executed using the NEB Golden Gate Assembly Kit ...
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bioRxiv - Microbiology 2021Quote: ... The PCRs were performed with a high-fidelity DNA polymerase (PrimeStarMax, Takara).
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bioRxiv - Plant Biology 2023Quote: ... Phusion High-Fidelity DNA Polymerase (Thermo) or CloneAmp HiFi PCR Premix (Takara) was used to PCR amplify all the above sequences with primers listed in Table S2 ...
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bioRxiv - Microbiology 2024Quote: ... The amplification was performed using high-fidelity PrimeSTAR GXL DNA polymerase (Takara). Next ...
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bioRxiv - Neuroscience 2022Quote: ... Pgcl2 and Pgcl3 cDNAs in male rat livers were amplified by PCR (PrimeSTAR DNA Polymerase Takara, Japan). The oligonucleotide primer sequences employed for this purpose are shown in Supplementary Table S1 ...
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bioRxiv - Microbiology 2022Quote: Protein-protein interactions were assayed with the Matchmaker yeast two-hybrid system (Clontech, Mountain View, CA). ORFs of AoHse was amplified from first-strand cDNA of A ...
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bioRxiv - Neuroscience 2020Quote: Enhanced green fluorescent protein (EGFP, Clontech), Tag-blue fluorescent protein (Tag-BFP ...
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bioRxiv - Molecular Biology 2022Quote: ... protein was estimated using BCA (TaKaRa), and 100 µg protein was incubated with 20 µl packed protein G-sepharose beads (Sigma P3296 ...
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bioRxiv - Neuroscience 2023Quote: ... 5) cDNA coding eGFP protein (Clontech). 6 ...
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bioRxiv - Evolutionary Biology 2022Quote: ... The α1-subunit genes were inserted at the PH promoter of vectors already containing the corresponding β1-subunit proteins using In-Fusion® HD Cloning Kit (Takara Bio, USA Inc.) and control sequenced ...
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bioRxiv - Biochemistry 2023Quote: ... The PCR fragments of spike protein DNA were cloned into linearized pMRNAXP vector using In-Fusion® HD Cloning Kit (Clontech® Laboratories, Inc.). The cloning mixtures were transformed to One Shot™ TOP10 Chemically Competent E ...
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bioRxiv - Biochemistry 2021Quote: ... an aliquot of the lysate was used to measure protein concentration by BCA protein assay (Takara Bio). The lysate was mixed with Optiphase Hisafe 3 (PerkinElmer) ...
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bioRxiv - Plant Biology 2020Quote: ... The cDNA was amplified using high-fidelity PrimeSTAR® Max DNA Polymerase (TaKaRa) followed by PCR clean-up ...
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bioRxiv - Microbiology 2021Quote: ... Allele fragments were generated using the high-fidelity PrimeSTAR Max polymerase (Takara Bio), assembled into strain-specific pEXG2 plasmids in E ...
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bioRxiv - Cell Biology 2021Quote: ... as two overlapping fragments using high-fidelity Takara LA Taq (Takara, Cat # RR002M). Primer details can be found in Supplementary Table 2 ...
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bioRxiv - Systems Biology 2021Quote: ... all PCRs utilized a high fidelity polymerase (CloneAmp HiFi PCR Premix, Takara 639298). Plasmids contained components of the synthetic inducible system flanked by homology to the intended genomic integration locus and were restricted prior to transformation ...
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bioRxiv - Genetics 2022Quote: ... Extraction of high-molecular-weight DNAs was performed using NucleoBond HMW DNA (TAKARA). Library preparation was performed using the Ligation Sequencing Kit (Oxford Nanopore Technologies ...
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bioRxiv - Plant Biology 2022Quote: ... purified with a high-salt solution for precipitation (Takara Bio Inc., Ohtsu, Japan) and reverse transcribed with ReverTra Ace (Toyobo) ...
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bioRxiv - Neuroscience 2020Quote: The screen of a rat brain cDNA library was carried out with the GAL4-based MATCHMAKER system (Clontech) and all procedures followed the system protocols ...
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bioRxiv - Neuroscience 2023Quote: EGFP-tagged rat PKCδ cDNA were cloned into EcoRI and BamHI digested pRetroX-TetOne-Puro (#634307, Takara Bio) using In-Fusion cloning system (#639648 ...
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bioRxiv - Plant Biology 2021Quote: Protein-protein interactions via yeast-two-hybrid (Y2H) assays were performed as described in the Matchmaker protocol (Clontech). HvRACB (amino acids 1-193 ...
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bioRxiv - Biochemistry 2023Quote: ... Uncleaved protein and cleaved His6-Ztag domains were separated from cleaved protein by incubation with TALON resin (Clontech). The supernatant was buffer exchanged to MES A buffer (20 mM MES ...
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bioRxiv - Microbiology 2020Quote: Tissue protein lysates were purchased from Takara. Details for each lysate are shown in Table S1 ...
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bioRxiv - Neuroscience 2020Quote: ... cell-free protein expression system from Takara Bio Inc ...
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bioRxiv - Biochemistry 2021Quote: ... Protein was purified using Talon resin (Clontech), eluted in 200 mM imidazole ...
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bioRxiv - Zoology 2020Quote: ... The protein concentration was measured by TaKaRa BCA Protein Assay Kit (Takara Bio ...
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bioRxiv - Molecular Biology 2019Quote: ... and protein was incubated with Talon (Clontech), 0.5 ml resin per litre cell culture for 1 h at 4°C ...
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bioRxiv - Cell Biology 2020Quote: ... the Capturem Protein A technology from Takara was employed according to the manufacturer’s protocol ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... subtilis Secretory Protein Expression System (TAKARA Bio). For signal peptide library transformation into B ...
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bioRxiv - Genetics 2019Quote: For high fidelity PCR reactions we followed manufacturer’s instructions using either Extaq (TaKaRa # RR001C), KOD hotstart (Merck Millipore # 71086 ...
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bioRxiv - Zoology 2021Quote: ... High-fidelity Takara Ex Taq PCR reagents were employed (Takara Bio Inc., Otsu, Japan) according to the manufacturer’s instructions ...
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bioRxiv - Zoology 2019Quote: ... High-fidelity Takara Ex Taq PCR reagents were employed (Takara Bio Inc., Otsu, Japan) according to the manufacturer’s instructions ...
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bioRxiv - Plant Biology 2023Quote: ... Primer Star high fidelity DNA polymerase and restriction enzymes were purchased from TaKaRa (Beijing). Ordinary Taq enzyme was purchased from Tiangen (Beijing).
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bioRxiv - Cancer Biology 2024Quote: High-quality RNA was isolated from cells or tissues using Trizol reagent (AJF1807A, Takara) according to the manufacturer’s instructions ...
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bioRxiv - Neuroscience 2022Quote: ... Gαi2 and Trpc2 cDNAs were amplified by PCR from female rat VNOs (PrimeSTAR® GXL DNA Polymerase Takara, Japan). Vom1r68 was synthesized by BGI (China) ...
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bioRxiv - Biochemistry 2023Quote: ... The membrane was incubated for 1 h at room temperature with Odyssey blocking buffer and 0.2 % PBS-T (PBS containing 0.2 % (v/v) Tween-20) with rat anti-HA clone 3F primary antibody (Clontech) at 1:1,000 dilution ...
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bioRxiv - Molecular Biology 2023Quote: Expression plasmids for fusion proteins of GFP and tardigrade proteins were constructed by Gibson assembly into pEGFP-N1 (Clontech) of the tardigrade cDNA (obtained by gene synthesis from Integrated DNA ...
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bioRxiv - Molecular Biology 2020Quote: A codon-optimized synthetic gene corresponding to the full length nucleocapsid protein (Thermo GeneArt, Regensburg, Germany) was cloned into a modified pET28a vector using the In-Fusion cloning kit (Takara Bio Saint-Germain-en-Laye, France). The resulting plasmid encoded for an N-terminal His6 tag followed by thrombin and tobacco etch virus cleavage sequences upstream of the full-length nucleocapsid protein sequence ...
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bioRxiv - Cell Biology 2019Quote: ... and both proteins were separated from each other and from a protein encoded by blasticidin resistance gene (cloned from pQCXIB #631516, Clontech) by self-cleavage peptide P2A ...
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Osh6 requires Ist2 for localization to the ER-PM contacts and efficient phosphatidylserine transportbioRxiv - Cell Biology 2020Quote: For testing protein-protein interactions Matchmaker™ GAL4 Two-Hybrid System 3 was used according to the manufacturer’s instructions (Clontech). Briefly ...
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bioRxiv - Cancer Biology 2024Quote: ... Eukaryotic expression vectors encoding green fluorescent protein (GFP)-tagged proteins were generated by inserting PCR-amplified fragments into pd1-EGFP-N1 vector (6073-1, Clontech). Prokaryotic plasmids encoding GST-fusion proteins were constructed using pGEX-4T-1 bacterial expression vector (27-4580-01 ...
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bioRxiv - Genetics 2019Quote: ... liver and kidney), mouse tissues (brain, liver, heart, testis and kidney) and rat tissues (brain, liver and kidney) were purchased from Takara Bio Inc.
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bioRxiv - Physiology 2021Quote: ... Brain sections were then incubated overnight at room temperature in blocking solution containing primary antiserum (rat anti-mCherry, Life Technologies M11217, 1:1,000; rabbit anti-dsRed, Clontech 632496 ...
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bioRxiv - Neuroscience 2023Quote: ... Full-length and truncated Cntn1 was PCR amplified from rat contactin-myc 34 and ligated together using an In-Fusion Snap Assembly Master Mix (Takara). All DNA constructs were verified by sequencing (Genewiz and plasmidsaurus).
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bioRxiv - Cancer Biology 2021Quote: High-throughput quantitative PCR (qPCR) was performed using the SmartChip system (Takara Bio, Kusatsu, JAPAN) on 47 oral melanoma samples to detect focal amplifications on tumor DNA on CFA 10 and CFA 30 ...
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bioRxiv - Synthetic Biology 2020Quote: ... including the promoter and the full CDS was amplified by high-fidelity PCR (TaKaRa Bio) and sequenced on an Illumina MiSeq system with transposase based library preparation kit (Nextera XT ...
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bioRxiv - Genetics 2020Quote: ... used as the template for PCR amplification with high fidelity polymerase (RR006Q, Takara, Tokyo, Japan). Alternatively ...
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bioRxiv - Microbiology 2022Quote: ... The resulting plasmid was transfected into 293T cells using Lentiviral High Titer Packaging Mix (Takara) to obtain the lentiviral vector for TSG101–venus transduction ...
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bioRxiv - Evolutionary Biology 2022Quote: ... A 2nd PCR was performed with high-fidelity PrimeSTAR GXL DNA Polymerase (Takara Cat: R050B) to add the Nextera indices (Nextera XT primers Set A 96 Indexes ...