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Citations for Takara Bio :
101 - 150 of 5049 citations for Rat High Mobility Group Protein B1 HMGB1 ELISA Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2021Quote: ... An anti-histidine antibody was used for ELISAs as positive control (Takara, catalog # 631212).
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bioRxiv - Cancer Biology 2022Quote: The total RNA from the brain tissues of all experimental groups were isolated using TRIZOL reagent (Takara Bio, Shiga, Japan) and the respective complementary deoxyribonucleic acid (c-DNA ...
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bioRxiv - Cell Biology 2021Quote: ... using Lentiviral High Titer Packaging Mix (Takara) as described previously75 ...
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bioRxiv - Plant Biology 2020Quote: ... After 1 μg of total RNA from leaves was reverse-transcribed using a High Fidelity PrimeScript™ RT-PCR Kit (Takara, Japan), and Real-time qRT-PCR was performed using a Thermal Cycler Dice® Real Time System III T950 (Takara ...
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bioRxiv - Neuroscience 2020Quote: ... One microgram of purified RNA from each sample was used for reverse transcription reaction to generate cDNA with a high-capacity cDNA reverse transcription kit (Takara, Dalian, China) and the resulting cDNA was then used for qPCR on a real-time PCR detection system (Bio-Rad ...
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bioRxiv - Cell Biology 2022Quote: ... cDNA were amplified from the embryo RNA using PrimeScript™ II High Fidelity One Step RT-polymerase chain reaction (PCR) Kit (Takara, R026A) using the following primers ...
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bioRxiv - Microbiology 2023Quote: Standards for determining copy number of virus stock were prepared using the PrimeScript II High Fidelity One Step RT-PCR Kit (TaKaRa, Cat# R026A) with IAV (H1N1 ...
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bioRxiv - Neuroscience 2022Quote: ... EGFP-positive cells from control and Yap1-5SA groups were directly collected into ice-cold lysis buffer (0.2 %Triton X-100, containing RNAse inhibitors, Clontech, cat# 635013) in 96-well plates (Eppendorf ...
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bioRxiv - Biochemistry 2019Quote: ... 500 µg protein at 1 mg/mL were prepared with Buffer A (Phosphoprotein Kit, Clontech, Cat#635626): 185 µL PC-3 lysate (pH 8.8) ...
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bioRxiv - Developmental Biology 2020Quote: ... Both chicken DLX1 and DLX1 Q50E proteins were purified using Capturem His-Tagged Purification Maxiprep Kit (Clontech), and verified by Western blot with primary antibodies against 6x His tag (Invitrogen ...
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bioRxiv - Developmental Biology 2023Quote: ... high-quality cDNA was generated from the total RNA using the SMART-Seq v4 Ultra Low Input RNA Kit (Takara Bio, Cat. #634889), and RNA-seq libraries were generated using the SMARTer ThruPLEX DNA-Seq Kit (Takara Bio ...
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bioRxiv - Neuroscience 2019Quote: Rat HRI was cloned into the Tet on-off system from Clontech (Tet-On® 3G Bidirectional Inducible Expression System with ZsGreen1 ...
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bioRxiv - Neuroscience 2022Quote: ... rat anti-GFP (1:200;) and rabbit anti-dsRed (1:600; Clontech). The anti-GFP and anti-dsRed antibodies were used to amplify the intrinsic fluorescent signals in the Ccr2RFP/+fmsEGFP/+ mice ...
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bioRxiv - Synthetic Biology 2023Quote: ... a high-fidelity DNA polymerase (PrimeSTAR GXL, Takara) and purified using a DNA Clean and Concentrator kit (Zymo Research) ...
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bioRxiv - Molecular Biology 2020Quote: ... qRT-PCR was carried out to evaluate the syndecan-4 expression between lenti-synd4 shRNA and lenti-null group with SYBP Premix Ex Taq TM from Takara (Shiga, Japan) following the manufacturer’s instructions ...
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bioRxiv - Genomics 2022Quote: ... For the Rubicon Genomics ThruPLEX® DNA-seq Kit: High Performance Library Preparation for Illumina® NGS Platforms (currently available from Takara Bio USA), the manufacturer’s protocol was followed.
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bioRxiv - Microbiology 2021Quote: ... The DNA fragments amplified by high fidelity PCR (Takara) were digested with the appropriate restriction enzymes ...
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bioRxiv - Microbiology 2020Quote: ... using Lenti-X high-titer lentiviral packaging systems (Clontech). The cell culture supernatant was harvested after 48 h and 72 h ...
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bioRxiv - Neuroscience 2023Quote: ... high-fidelity polymerase (Cat#R050A, Takara Bio USA, Inc.) as per manufacturer’s instructions ...
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bioRxiv - Plant Biology 2023Quote: ... High fidelity Taq polymerase (PrimeSTAR GXL DNA polymerase, Takara) was used in the preparation of the PCR reaction mixture followed by the PCR program according to Gayatri et al ...
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bioRxiv - Genetics 2023Quote: ... High-quality total RNA was purchased from Takara (www.takarabio.com). Total RNA was isolated by a guanidinium thiocyanate method.27 Total RNA concentration ...
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bioRxiv - Immunology 2020Quote: Levels of p24 antigen in the purified SARS-CoV-2 pseudotype solution was measured by ELISA (Takara). Mouse sera were heat inactivated ...
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bioRxiv - Microbiology 2020Quote: ... Total protein was extracted from 2,000 midguts using the lysis buffer supplied in the Capturem IP & Co-IP kit (Takara, 635721). The extracted proteins were divided into two equal parts and used for immunoprecipitation (IP) ...
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bioRxiv - Biophysics 2020Quote: pDNA_HaloTag/SNAP-tag/GFP vectors encoding each target protein (see Note 1),which were inserted by In-Fusion HD Cloning Kit (Clontech) or Seamless Ligation Cloning Extract (SLiCE ...
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bioRxiv - Microbiology 2022Quote: ... and E484Q mutations of the SARS-CoV-2 spike protein were determined by qPCR using mutation detection kits purchased from Takara Bio (Shiga ...
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bioRxiv - Synthetic Biology 2020Quote: ... High fidelity polymerase (PrimeSTAR GXL, Takara Bio Inc, Shiga, Japan) was used for PCR ...
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bioRxiv - Developmental Biology 2023Quote: ... LA Taq® high fidelity DNA polymerase (TaKaRa, Catalog #RR002A) was used to amplify the CRISPR-Cas9 target site according to the manufacturer’s instructions ...
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bioRxiv - Plant Biology 2020Quote: ... All of the other genes encoding candidate RBOHD-associated proteins were cloned into epiGreenB5 by the same strategy using an In-Fusion HD Cloning Kit (Clontech, USA). To generate epiGreenB-pPB1CP::PB1CP-3-eGFP for transient expression in N ...
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bioRxiv - Microbiology 2020Quote: ... Ad5 stocks were tittered by detection of the Ad hexon protein using Adeno-X™ Rapid Titer Kit (Cat. No. 632250, Takara, Clontech). The Ad5 CPE was dose-dependent ...
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bioRxiv - Biochemistry 2022Quote: ... cells were transfected with plasmids expressing either SARS-COV-2 S protein WT or mutants (E1182K, L1193G, E1182K/L1193G, L1182K/L1186G/L1193G) by using Calcium phosphate transfection kit (Takara-Bio). 24 h post transfection ...
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bioRxiv - Molecular Biology 2022Quote: ... genes were cloned into a His-SUMO fusion protein expression vector (Kim et al, 2018) using an In-Fusion Cloning Kit (639648, Takara Bio). The glycine-arginine-proline (GRP ...
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bioRxiv - Genetics 2019Quote: ... CG13025 was amplified with the high-fidelity polymerase PrimeSTAR HS (Takara) and sequenced by Sanger sequencing (Eton) ...
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bioRxiv - Molecular Biology 2019Quote: ... Q5 High-Fidelity and Taq DNA polymerases were purchased from Takara Bio USA (Mountain View ...
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bioRxiv - Genetics 2020Quote: ... PCR was performed using high-fidelity Prime STAR Polymerase (TaKaRa, Japan) under the following conditions ...
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bioRxiv - Immunology 2022Quote: ... was amplified using a high-proof reading polymerase (CloneAmp HiFi, Takara) with primers containing EcoRI and NotI restriction sites and subcloned into pCR blunt II TOPO vector for subsequent restriction and ligation into the pcDNA3 expression vector ...
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bioRxiv - Microbiology 2020Quote: ... D and E using high fidelity PrimeSTAR Max DNA Polymerase (Takara). T7 promoter was introduced upstream of 5’ UTR of SARS-CoV-2 genome in fragment A ...
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bioRxiv - Microbiology 2022Quote: ... with the help of high fidelity PrimeSTAR GXL DNA Polymerase (Takara). The PCR amplicon was cloned in the pGEMT-easy plasmid (Stratagene ...
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bioRxiv - Cell Biology 2023Quote: ... were co-transfected with Lentiviral High Titer Packaging Mix (Takara Bio) and the lentiviral plasmid (pLVSIN-PIP-FUCCI or pBOB-EF1-FastFUCCI (Addgene ...
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bioRxiv - Bioengineering 2023Quote: ... The high fidelity PrimeSTAR GXL DNA Polymerase (Takara Bio Inc., Japan) and the Hot start GoTaq polymerase (Promega Corporation ...
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bioRxiv - Microbiology 2020Quote: ... Proteins were tagged with Gfp (enhanced green fluorescent protein; Clontech, Mountain View, CA, USA) at their N-terminus unless noted otherwise ...
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bioRxiv - Developmental Biology 2021Quote: ... and recombinant Cas9 proteins (Clontech) were introduced together into the KhES-1 cells by electroporation (Neon device ...
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bioRxiv - Molecular Biology 2023Quote: ... The recombinant Cas9 protein (TAKARA) and the sgRNA were incubated for 10 min at 37°C ...
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bioRxiv - Microbiology 2020Quote: ... Ad5 stocks were tittered by detection of the Ad hexon protein using Adeno-X™ Rapid Titer Kit (Cat. No. 632250, Takara, Clontech). The Ad5 CPE was dose-dependent ...
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bioRxiv - Biochemistry 2023Quote: ... Expression plasmids for other His-mCherry or His-GFP fusion proteins were constructed using in-Fusion HD Cloning Kit (Takara Bio, Inc.). All mCherry/GFP fusion proteins were expressed in E ...
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bioRxiv - Molecular Biology 2019Quote: Total RNA samples were isolated from the rat liver using RNAiso Plus (D9109; TaKaRa, Dalian,China). RNA samples were converted to cDNA using a RevertAid First Strand cDNA Synthesis Kit (Thermo Fisher Scientific ...
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bioRxiv - Neuroscience 2021Quote: ... Ank only) rat Shank3 with a C-terminal mRFP-tag were generated in pmRFP-N3 (Clontech). A construct coding for N-terminally GFP-tagged full-length rat Shank3 in the pHAGE vector was obtained from Alex Shcheglovitov (Univ ...
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bioRxiv - Neuroscience 2021Quote: ... rat Txn1 cDNAs were amplified by PCR using LA-Taq polymerase (Takara Bio Inc, Shiga, Japan) with FW-primer ...
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bioRxiv - Molecular Biology 2023Quote: We extracted total RNA from rat cells and tissues employing the TRIzol reagent (D9108A, TaKaRa Bio). The RNA was then reverse-transcribed into complementary DNA (cDNA ...
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bioRxiv - Molecular Biology 2021Quote: Secretion in PIP in NHLFs treated with MRG-201 or MRG-229 was conducted by collecting the supernatant of treated cells and performing procollagen type I PIP ELISA (Takara).
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bioRxiv - Biophysics 2019Quote: ... cDNAs encoding cyan fluorescent protein (CFP) and yellow fluorescent protein (YFP) were purchased from Clontech Com ...