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Citations for Takara Bio :
301 - 350 of 945 citations for Rat FAM24A shRNA Plasmid since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Plant Biology 2021Quote: ... The resulting plasmid was then used for In-Fusion reaction (Takara Clontech) to insert PCR-amplified GFP fragments at the C-terminus of the SCL28 coding sequence ...
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bioRxiv - Neuroscience 2019Quote: ... and subcloned into the Cry2.mCh mammalian expression plasmids (mCherry-N1 (Clontech)) via restriction digest and ligation ...
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bioRxiv - Microbiology 2021Quote: ... were co-transformed with the plasmids pET-28b_atsJ and pG-KJE8 (TAKARA). A 200 ml culture in TB medium (50 μg/ml kanamycin ...
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bioRxiv - Microbiology 2020Quote: ... Transformation with plasmids and cultivation were performed using standard techniques (Clontech manual). In addition to negative and positive controls included in the MatchMaker III system ...
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bioRxiv - Cell Biology 2020Quote: The following plasmids were used in this study: pCDNA (control vector) (Clontech), pCDNA3-EGFP (Addgene Plasmid #13031) ...
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bioRxiv - Microbiology 2022Quote: ... and the PBSU101 plasmid was digested by BamHI and XbaI (Japanese Takara). Then PBSU101-pykF ...
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bioRxiv - Cancer Biology 2022Quote: The retroviral plasmid pRetroX-TetOne puro (634307, Clontech, Mountain View, CA, USA) was amplified using NEB Stable competent Escherichia coli (high efficiency ...
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bioRxiv - Immunology 2022Quote: ... Ligated plasmids were then transformed into Stellar competent cells (Takara Clontech, 636763) and single clones were picked on the next day ...
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bioRxiv - Immunology 2022Quote: ... Ligated plasmids were then transformed into Stellar competent cells (Takara Clontech, 636763) and single clones were picked on the next day ...
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bioRxiv - Neuroscience 2019Quote: ... The cloned plasmids were transformed into Stellar competent cells (Takara Bio, Japan), and 200 clones per rabies virus sample were isolated and purified for sequencing ...
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bioRxiv - Neuroscience 2019Quote: ... Plasmids used in this study were: pEGFP-N1 (Clontech, Mountain View, California); Flag-CNTNAP2 (generated by subcloning human CNTNAP2 cDNA from a plasmid kindly provided by Dr ...
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bioRxiv - Cell Biology 2019Quote: pEGFP-N1 (EGFP) and mCherry-C1 (mCherry) plasmids were purchased from Clontech Laboratories ...
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bioRxiv - Biochemistry 2019Quote: Recombinant DmNobo was expressed with the pCold-III plasmid vector (TaKaRa Bio) in Escherichia coli strain BL21(DE3 ...
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bioRxiv - Cell Biology 2020Quote: ... The sequence was ligated into the pLVX Puro lentiviral plasmid (Clontech, CA) using the same restriction sites and sequenced to confirm correct orientation ...
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bioRxiv - Cell Biology 2021Quote: ... Plasmids were constructed using In-Fusion HD EcoDry Cloning Kits (Takara 121416), NEB HiFi assembly (New England Biolabs E5520S) ...
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bioRxiv - Immunology 2020Quote: The pLVX-IRES-ZsGreen1 plasmid (Takara Bio USA, Inc., Mountain View, CA) was used to generate construct encoding BiTE cDNA ...
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bioRxiv - Microbiology 2021Quote: ... and then was cloned in the plasmid pMD19-T simple (TAKARA, Japan). The DNA fragment was digested with EcoRI/XhoI and BamHI/EcoRI (Thermo Fisher Scientific ...
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bioRxiv - Molecular Biology 2022Quote: ... The assembled plasmids were amplified by transformation of Stellar Competent Cells (Takara) and purified from overnight cultures in LB medium containing 100 μg/ml Ampicillin grown at 37°C by using the NucleoSpin Plasmid isolation kit (Macherey-Nagel) ...
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bioRxiv - Systems Biology 2022Quote: ... with pMI-NLS-Cre-ires-Tomato plasmid using the CalPhos kit (Clontech). Retroviral supernatants were harvested after 48hrs ...
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bioRxiv - Plant Biology 2023Quote: ... The recombinant plasmids were co-transformed into yeast Y2H Gold strain (Takara). The transformed yeast strains were grown on DDO (SD/-Leu/-Trp ...
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bioRxiv - Microbiology 2023Quote: ... and pMD2.G plasmids using TransIT-293 Transfection Reagent (TaKaRa, Cat# V2700) in Opti-MEM I Reduced Serum Medium (Thermo Fisher Scientific ...
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bioRxiv - Cell Biology 2023Quote: TurboID plasmids were made using In-Fusion Recombination (Takara Bio USA, Inc.). 3xHA-TurboID was amplified from 3xHA-TurboID-NLS pcDNA3 (Addgene plasmid #107171 ...
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bioRxiv - Cell Biology 2023Quote: ... and inserted into the pGEMHE plasmid backbone (In-fusion kit; Takara; 638948). Each CENP-O construct was tagged with GFP at the C-terminus ...
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bioRxiv - Cell Biology 2023Quote: ... plasmids were co-transfected into Lenti-XTM 293T cells (Clontech Laboratories, 632180) using Lipofectamine 3000 (Fisher ...
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bioRxiv - Developmental Biology 2023Quote: ... Addgene plasmid #17398) digested with EcoRI using In-Fusion HD system (Clontech). The fragments of interest were PCR amplified using high-fidelity Pfu DNA polymerase (ThermoFisher Scientific) ...
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bioRxiv - Cell Biology 2023Quote: ... the plasmid was constructed using the In-Fusion HD Cloning Kit (Takara); 1–2 kb sequences of the 5’ and 3’ ends of the genes of interest flanked the fragment that consisted of an in-frame linker ...
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bioRxiv - Plant Biology 2023Quote: ... the plasmid was constructed using the In-Fusion HD Cloning Kit (Takara); 1–2 kb sequences of the 5’ and 3’ ends of the genes of interest flanked the fragment that consisted of an in-frame linker ...
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bioRxiv - Microbiology 2023Quote: ... and pMD2.G plasmid with TransIT-293 Transfection Reagent (TaKaRa, Cat# V2700) in Opti-MEM (Thermo Fisher Scientific ...
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bioRxiv - Cell Biology 2023Quote: ... Plasmids were transfected in HEK by using Xfect Transfection Reagent (631318, Takara) by following the user manual.
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bioRxiv - Microbiology 2024Quote: Plasmids were introduced into HEK293T cells using the TransIT®-293 (Takara) reagent in six-well plates ...
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bioRxiv - Genetics 2019Quote: ... liver and kidney), mouse tissues (brain, liver, heart, testis and kidney) and rat tissues (brain, liver and kidney) were purchased from Takara Bio Inc.
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bioRxiv - Physiology 2021Quote: ... Brain sections were then incubated overnight at room temperature in blocking solution containing primary antiserum (rat anti-mCherry, Life Technologies M11217, 1:1,000; rabbit anti-dsRed, Clontech 632496 ...
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bioRxiv - Neuroscience 2023Quote: ... Full-length and truncated Cntn1 was PCR amplified from rat contactin-myc 34 and ligated together using an In-Fusion Snap Assembly Master Mix (Takara). All DNA constructs were verified by sequencing (Genewiz and plasmidsaurus).
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bioRxiv - Plant Biology 2020Quote: ... The recombinant plasmids were transformed into Stellar™ Competent Cells (Clontech, Catalog # 636763), and positive colonies were selected on LB plates containing spectinomycin (100ug/mL ...
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bioRxiv - Biophysics 2021Quote: ... Five plasmids designed to express the following “chaperone teams” were purchased from TAKARA Bio Inc ...
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bioRxiv - Developmental Biology 2021Quote: ... Plasmid construction was performed using Infusion cloning enzymes (Takara Bio, Mountain View, CA).
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bioRxiv - Neuroscience 2022Quote: ... for QF2 lines and NucleoBond Xtra Midi Endotoxin-Free plasmid kit (Clontech 740420.50) for Split-QF2 lines and eluted in ultrapure water prior to injection ...
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bioRxiv - Neuroscience 2021Quote: ... The backbone plasmid used to transfect cell lines was pIRES2-EGFP2 (Clontech, UK). Coverslips were fixed with 4% paraformaldehyde (PFA ...
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bioRxiv - Molecular Biology 2020Quote: ... Sequence was then inserted in the plasmid pEGFP-C1 (Clontech, Mountain View, CA) at Sma1 site ...
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bioRxiv - Cell Biology 2022Quote: ... Digestion and ligation to BamHI and SalI-linearized pColdI plasmid (Takara, Shiga, Japan) were performed ...
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bioRxiv - Molecular Biology 2021Quote: ... Mutations were introduced by whole-plasmid PCR using PrimeSTAR MAX DNA polymerase (Takara) according to the manufacturer’s protocol ...
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bioRxiv - Genetics 2019Quote: ... Pairs of plasmids were transformed into strain Y187 (Clontech, Mountain View, CA, USA) and liquid β-galactosidase assays were performed using an OMEGASTAR plate reader (BMG Labtech ...
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bioRxiv - Biophysics 2019Quote: ... and the mCherry gene was obtained from the pmCherry-N1 plasmid (Takara Bio). Reaction products were transformed ...
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bioRxiv - Biochemistry 2020Quote: ... ADAR1 and PCBP2 cDNA were cloned from human thymus plasmid cDNA library (Clontech) using standard PCR techniques and then subcloned into indicated vectors ...
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bioRxiv - Microbiology 2021Quote: ... fragments were PCR amplified from the plasmids using PrimeSTAR GXL Polymerase (Takara, Japan) and Nhel_IDT_F and EcoRI_ITD_R primers (Supplementary table 7) ...
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bioRxiv - Cell Biology 2022Quote: ... GFP-tagged PDE5A isoforms plasmids [19] were co-transfected with pDsRed-mito (Clontech) into HEK293 cells using Lipofectamine 3000 (Life Technologies ...
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bioRxiv - Plant Biology 2021Quote: ... The recombinant plasmid was transformed into Stellar™ Competent Cells (Clontech, Catalog # 636763) according to the manufacturer’s protocol and positive colonies were selected on LB plates containing kanamycin (50 μg/mL).
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bioRxiv - Cell Biology 2021Quote: ... Plasmid DNA was transfected into A549 cells by Xfect transfection reagent (Takara Bio), while transfection of ON-Targetplus siRNAs (Dharmacon ...
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bioRxiv - Genetics 2019Quote: ... HEK293T cells were transfected with wildtype or mutant SCN5A pIRES2-EGFP plasmids (Clontech) using FuGENE 6 (Promega ...
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bioRxiv - Neuroscience 2019Quote: ... Concentrated lentiviruses for these two plasmids were made using Lenti-X concentrator (Takara) as has been done previously (Sun et al. ...