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Citations for Takara Bio :
451 - 500 of 945 citations for Rat FAM24A shRNA Plasmid since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2022Quote: ... The resulting plasmid was transfected into 293T cells using Lentiviral High Titer Packaging Mix (Takara) to obtain the lentiviral vector for TSG101–venus transduction ...
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bioRxiv - Microbiology 2023Quote: ... USA) and subcloned into the NheI and XhoI restriction sites of plasmid pIRES2-EGFP (Clontech, Takara Bio USA ...
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bioRxiv - Biophysics 2023Quote: ... The plasmid DNA was cut with BamH1 to a linear DNA (Takara Biomedical Technology (Beijing)) ...
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bioRxiv - Systems Biology 2023Quote: ... Ligated plasmid products were transformed into stellar competent cells (E. coli HST08 strain, Takara Bio). See Table S1 for full descriptions of constructs.
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bioRxiv - Cancer Biology 2023Quote: ... The TOB1-AS1 overexpression plasmid was amplified by transforming Stellar™ Competent Cells (Takara, 636763) with the plasmid as per instructions and isolated by QIAGEN HiSpeed Plasmid Midi Kit (QIAGEN ...
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bioRxiv - Developmental Biology 2023Quote: ... to remove the template plasmid DNA and then self-ligated using Ligation High Ver2 (Takara). All PCR products and enzyme-digested vectors were purified using QIAquick PCR Purification Kit (Qiagen) ...
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bioRxiv - Evolutionary Biology 2023Quote: ... coli BL21 (DE3) in the presence of GroEL/ES chaperones (pGro7 plasmid, TAKARA Bio, USA). After a pre-screen on agar plates ...
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bioRxiv - Cell Biology 2023Quote: ... 1.6 µg of a transfer plasmid was transfected into LentiX 293T cells (Clontech Laboratories, inc.) with 0.8 µg of psPAX2 (addgene #12260 ...
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bioRxiv - Synthetic Biology 2024Quote: ... The inducible ABE plasmid was made by integrating a tet-responsive promoter (TRE3G, Takara Bio) and ABE 7.1050 (Addgene #102919 ...
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bioRxiv - Bioengineering 2024Quote: ... and the receptor- or payload-encoding viral expression plasmids (2.0 μg) into Lx293T cells (Clontech) in 6-well plates with Lipofectamine 3000 (Thermo Fisher L3000001) ...
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bioRxiv - Cancer Biology 2024Quote: ... Caspase 8 reporter was established by replacing the DEVD amino acid sequence with XEXD in pLenti_CMV_GFP_Blast_FLIP_GFP_DEVD plasmid through the In-fusion Snap assembly master mix (Takara Bio) using the following oligos forward ...
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bioRxiv - Cancer Biology 2024Quote: 1 x 106 ACKP cells were transfected with 3 µg pE2F-TA-luc plasmid (Takara) and 0.3 µg renilla luciferase control plasmid pRL-CMV (Promega ...
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bioRxiv - Genetics 2024Quote: ... wherein 2.5 µg of pooled plasmid was diluted with Xfect reaction buffer (Takara Bio, 631318) to a total volume of 50 μL ...
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bioRxiv - Cell Biology 2024Quote: Following plasmids were used in this assay to generate stable cell lines: pCMV-HRasG12V (Clontech), Piggyback vectors ...
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bioRxiv - Microbiology 2020Quote: ... This DNA fragment was transformed into the ΔasgA strain along with the plasmid pPTR1 (TaKaRa Bio) containing the pyrithiamine resistance ptrA gene ...
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bioRxiv - Genetics 2020Quote: ... The linearized plasmid used for hydrogen peroxide treatment and library construction was cut by EcoRV (Takara) to generate blunt-ends ...
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bioRxiv - Molecular Biology 2021Quote: RNAi plasmids were constructed using the In-Fusion® HD Cloning Kit (Takara Bio; Cat #: 639650) and the L4440 vector ...
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bioRxiv - Neuroscience 2021Quote: ... Neuronal morphology of 14 DIV neurons transfected with the plasmid peGFP-N1 (Clontech, Mountain View, CA) was examined using high-resolution confocal digital images obtained from an Opera-Phenix microscope (Perkin-Elmer) ...
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bioRxiv - Neuroscience 2019Quote: ... and SV40 poly A signal (from the pTRE-tight plasmid from Clontech Laboratories, Mountain View, CA) sequences were cloned right after the 5’ homology fragment ...
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bioRxiv - Cell Biology 2019Quote: ... The mCherry-Golgi plasmid was made by transferring mCherry from pmCherry-N1 into pEYFP-Golgi (Clontech) using AgeI/NotI ...
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bioRxiv - Molecular Biology 2020Quote: ... and further ligated into BbsI digested pX330 plasmids by T4 DNA ligase (Takara; Kusatsu, Shiga, Japan). The ligate was transformed to DH5α competent cells for culture overnight ...
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bioRxiv - Cell Biology 2019Quote: ... This insert was ligated into the HindIII and SalI sites in the peGFP-C1 plasmid (Clontech). The eGFP and COMMD1 coding sequence was subcloned to pTre-TIGHT-Bi (Clontech ...
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bioRxiv - Microbiology 2020Quote: ... All modification for plasmids were performed using overlap PCR mutagenesis with InFusion cloning (Takara Biosciences, USA). Viral genome and support plasmids were maintained in chemically-competent Stbl2 E ...
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bioRxiv - Microbiology 2021Quote: ... This DNA fragment was transformed into the ΔkojR strain along with the plasmid pPTR1 (TaKaRa Bio) containing the pyrithiamine resistance ptrA gene ...
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bioRxiv - Microbiology 2021Quote: ... plasmids respectively using primers HindIII_ZIKV_sfRNA and XbaI_GFP (Supplementary table 7) and PrimeSTAR GXL Polymerase (Takara, Japan). Cycling conditions were as follows ...
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bioRxiv - Microbiology 2022Quote: ... The PCR product and pET28b plasmid were digested with restriction endonuclease EcoRI and HindIII (Japanese Takara), then ligated with T4 DNA Ligase (Japanese Takara) ...
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bioRxiv - Microbiology 2022Quote: ... the FLAG-APEX2-GARG-1060 insert was cloned into the retroviral vector plasmid pLNCX2 (Takara Bio). Cloning details are available upon request ...
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bioRxiv - Cell Biology 2022Quote: All expression plasmids are based on the lentiviral vector pLVX-EF1α-mCherry-N1 (Takara, Cat# 631986). Codon-optimized variants of GPR133 were previously published and described (Frenster et al. ...
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bioRxiv - Cancer Biology 2022Quote: ... DNA for experiments was obtained using endotoxin-free plasmid purification kits (NucleoBond Xtra Midi EF, Takara, cat ...
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bioRxiv - Cancer Biology 2022Quote: ... Recombinant AAV2 was packaged in HEK293T cells using pHelper and pRC2-mi342 plasmids (Takara, Catalog #632608). Three days after transfection ...
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bioRxiv - Molecular Biology 2020Quote: ... 2.5 μg of PrimPol variant expressing pcDNA3.1/nV5-DEST vectors11 or control plasmid pEGFP-C2 (Clontech) were transfected for the last 24 h using TransIT-2020 (Mirus Bio) ...
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bioRxiv - Synthetic Biology 2021Quote: ... Insert the purified array into the opened plasmid backbone using In-Fusion cloning (Takara Bio, Japan).
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bioRxiv - Biophysics 2021Quote: Full length mouse ILK cDNA was cloned into the EcoRI site of pEGFP-N1 plasmid (Clontech). The R255A ...
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bioRxiv - Neuroscience 2020Quote: ... PCR amplified and inserted into the DsRed-Express2 plasmid using in-fusion cloning (Takara Bio, CA). St3-Halo was generated by replacing the GFP in St3-eGFP with Halo tag (GenScript USA Inc ...
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bioRxiv - Microbiology 2021Quote: ... Plasmids were constructed using synthetic double-stranded DNA (Integrated DNA Technologies) and In-Fusion cloning (Takara) into the pL6 plasmid ...
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bioRxiv - Cell Biology 2019Quote: All plasmids were generated using standard restriction enzyme cloning and InFusion system (TaKaRa Bio, Kusatsu, Japan). The list of all plasmids used in the study is available in Table S1 ...
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bioRxiv - Cell Biology 2020Quote: ... The cloned plasmid was packaged into lentivirus using lenti-X-packaging-single-shots (VSVG) (Takara, #631275) according to the manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2021Quote: ... Deletion or substitution mutagenesis of these plasmids was performed using a PrimeSTAR Mutagenesis Basal Kit (Takara) and primers listed in Table S1 ...
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bioRxiv - Genetics 2022Quote: Full length wild-type or mutant BRC-1 sequences were cloned into plasmid pBridge (Takara Bio), transformed into yeast strain Y2HGold (Takara Bio ...
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bioRxiv - Genomics 2022Quote: ... and the bacterial lawn later scraped for plasmid extraction using NucleoBond Xtra Midi Plus (Takara Bio). The plasmid pool was then triple-digested with SphI-HF ...
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bioRxiv - Genomics 2022Quote: ... and the bacterial lawn later scraped for plasmid extraction using NucleoBond Xtra Midi Plus (Takara Bio). 20 individual bacterial colonies were separately picked from a dilution plating and miniprepped using Nucleospin Plasmid (Takara Bio) ...
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bioRxiv - Microbiology 2022Quote: ... the gene fragments of transcriptional regulators were cloned into the pColdII plasmid (Takara Bio, Kusatsu, Japan) and transformed into E ...
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bioRxiv - Genetics 2022Quote: ... 56 plasmids in 293FT HEK cells using the CalPhos™ Mammalian Transfection Kit (Takara bio, 631312). Following 2 μM tamoxifen treatment ...
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bioRxiv - Developmental Biology 2023Quote: ... This rtTA-advanced ORF was replaced with rtTA3G from the pCMV-Tet-3G plasmid (TaKaRa Bio), by XbaI and XmaI digestion and cloning of three separate fragments to create the final rtTA vector AAVS1-NEO-UBrtTA-3G.
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bioRxiv - Genomics 2023Quote: ... Recombinant AAV2 was packaged in HEK293T cells using pHelper and pRC2-mi342 plasmids (Takara, Catalog #632608). Three days after transfection ...
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bioRxiv - Molecular Biology 2024Quote: ... YCplac22 plasmids that carry tsa1 mutant alleles were constructed using the PrimeSTAR Mutagenesis Basal Kit (TaKaRa) according to the manufacturer’s instructions ...
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bioRxiv - Microbiology 2023Quote: Retrovirus plasmids were generated by restriction enzyme cloning of latency genes into pRetroX-IRES-ZsGreen (Takara). Latency locus genes were amplified from WT MHV68-BAC DNA127 and the frame-shift stop mutant ...
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bioRxiv - Bioengineering 2023Quote: ... Plasmid DNA from the Gibson assembly reaction was then transformed into Stellar Competent Cells (Takara Bio).
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bioRxiv - Biochemistry 2023Quote: The plasmids for producing XccOpgD mutants were constructed using a PrimeSTAR mutagenesis basal kit (Takara Bio) according to the manufacturer’s instructions ...
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bioRxiv - Microbiology 2024Quote: HEK293T cells were transfected with expression plasmids using the TransIT-293 transfection reagent (Takara, Shiga, Japan), following the manufacturer’s instructions.