Labshake search
Citations for Takara Bio :
301 - 350 of 366 citations for Rat Colony Assay Media since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Plant Biology 2021Quote: Protein-protein interactions via yeast-two-hybrid (Y2H) assays were performed as described in the Matchmaker protocol (Clontech). HvRACB (amino acids 1-193 ...
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bioRxiv - Microbiology 2022Quote: ... a one-step reverse transcription-PCR (RT-PCR) assay was conducted with a PrimeScript kit (Takara, Tokyo, Japan), and Viral genomic terminal sequences were determined using commercial 5’ and 3’ RACE (rapid amplification of cDNA ends ...
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bioRxiv - Molecular Biology 2020Quote: ... Cells were lysed and quantitative β-galactosidase assay was performed using ONPG substrate following standard protocols (Clontech Laboratories).
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bioRxiv - Plant Biology 2022Quote: ... For Y2H assays, cDNAs of mCherry and ZmSIPs (ZmSIP1, ZmSIP2, ZmSIP3) were cloned into the pGADT7 vector (Clontech) for expression via the GAL4 AD under constitutive ADH1 promoter ...
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bioRxiv - Cancer Biology 2024Quote: ... we employed the MTT (3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide) or WST-1 (Water-Soluble Tetrazolium 1) assay from Takara, Japan ...
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bioRxiv - Immunology 2021Quote: ... was used to for two-step qPCR assay after cDNA synthesis using PrimeScript® reverse transcriptase kit (Takara, Japan). Gene expression was normalized to the expression level of β-actin ...
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bioRxiv - Plant Biology 2021Quote: Yeast two-hybridization (Y2H) assays were performed according to the manufacture’s procedure (Clontech Laboratories Inc., Palo Alto, CA, USA). For each isozyme ...
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bioRxiv - Cell Biology 2022Quote: ... The yeast two-hybrid assays were performed according to the Matchmarker Gold Yeast two-hybrid system manual from Clontech. Different variations of the constructs were co-transformed by the polyethylene glycol/ lithium acetate method into the AH109 yeast strain and selected on SD/-Leu-Trp plates ...
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bioRxiv - Plant Biology 2019Quote: ... For Yeast Two-Hybrid assays RIPa was sucloned from the pGEM-T easy vector into pGADT7 plasmid (Clontech Laboratories) using the mentioned restriction sites ...
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Guidelines for accurate genotyping of SARS-CoV-2 using amplicon-based sequencing of clinical samplesbioRxiv - Genomics 2020Quote: ... CDC-USA assay targeting gene N (IDT # 10006713) and One Step PrimeScript™ III RT-PCR Kit (TaKaRa #RR600A). Serial dilutions of reference material were prepared ranging from 1 to ~10M genome equivalents per reaction ...
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RTEL-1 and DNA polymerase theta promote subtelomeric DNA synthesis and telomere fusion in C. elegansbioRxiv - Genetics 2022Quote: PCR for the ITS-DNA synthesis assay was carried out using ExTaq polymerase (Takara Bio cat# RR001A, Kasatsu, Japan). 35 cycles were executed with 64°C annealing temperature and 1 minute extension time ...
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bioRxiv - Plant Biology 2022Quote: Y2H protein interaction assays were performed according to the manufacturer’s instructions as described in the Yeast Protocols Handbook (Clontech; TaKaRa Bio USA ...
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bioRxiv - Neuroscience 2023Quote: TUNEL assays were performed to detect apoptotic cell death using Clonetech ApoAlert DNA Fragmentation kit (Takara, Kusatsu, Shiga, Japan) according to the manufacturer’s protocol ...
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bioRxiv - Molecular Biology 2023Quote: The Yeast Two-Hybrid assays were performed according to the Matchmaker Gold Yeast Two-Hybrid System (Cat#630489; Clontech) kit instructions ...
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bioRxiv - Plant Biology 2023Quote: ... The Y2H assay was carried out based with the manufacturer’s protocol of Matchmaker Gold Yeast Two-Hybrid System Kit (Takara). The primers used for vector construction are listed in Supplemental Data Set S1.
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bioRxiv - Plant Biology 2019Quote: For Yeast-two-Hybrid (Y2H) assays coding sequences were cloned into pDONR207 and recombined into the pGDAT7 and pGBKT7 (Clontech). Using the co-transformation techniques41 these constructs were transformed into the AH109 strain (Clontech).
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bioRxiv - Developmental Biology 2021Quote: Two-hybrid screening and assays were performed as described in the Matchmaker™ Pretransformed Libraries User Manual (Takara PT3183-1) using a Drosophila Mate & Plate™ Library - Universal Drosophila (Normalized A+ RNA isolated from embryo (~20 hr) ...
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bioRxiv - Molecular Biology 2021Quote: Nested translocation PCR assays was performed from genomic DNA as previously described (29) using either LA Taq DNA polymerase (Takara) or Flex HS polymerase (NEB) ...
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PPARγ is a tumor suppressor in basal bladder tumors offering new potential therapeutic opportunitiesbioRxiv - Cancer Biology 2019Quote: ... The GAL4 DNA-binding domain cloning vector pM and the activation-domain cloning vector pVP16 are part of the Mammalian Matchmaker Two-Hybrid Assay kit (BD Biosciences Clontech). The construct pM-MED1 (510-787 ...
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bioRxiv - Plant Biology 2020Quote: ... Plasmids for the PARN activity assay were constructed by inserting the coding sequence of RRD1 or human PARN (hPARN) into the pHAT vector (Clontech). The hPARN sequence was derived from the GNP Human cDNA clone IRAK071M01 (RIKEN BioResource Research Center) ...
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bioRxiv - Plant Biology 2021Quote: Yeast two-hybrid assays were performed as described (Cesari et al., 2013) using the Matchmaker Gold Yeast Two-hybrid system (Clontech).
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bioRxiv - Immunology 2022Quote: Lytic cell death was determined by measuring LDH release from cell-free supernatants using a colorimetric assay (Promega or Takara) according to the manufacturer’s protocol ...
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bioRxiv - Microbiology 2022Quote: ... Virus production was quantified by determining the amount of the Gag p24 protein using an enzyme-linked immunosorbent (ELISA) assay (Innogenetics or TaKaRa). For production of HIV-1 Env-pseudotyped viruses in Jurkat cells ...
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bioRxiv - Molecular Biology 2020Quote: ... Confirmatory “1-to-1” pairwise assays for selected interactants were performed with the MatchMaker Two-Hybrid System 3 (Clontech Inc.)
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bioRxiv - Genomics 2020Quote: Yeast one-hybridization assay was performed using the Matchmaker® Gold Yeast One-Hybrid System (Clontech, Palo Alto, CA, USA). The promoter sequence (upstream 2kb from the start codon ...
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bioRxiv - Microbiology 2020Quote: ... according to the manufacturer’s instructions and detected by RT-qPCR assays with a One-Step PrimeScript RT-PCR kit (Takara, Japan) using SARS-CoV-2-specific primers on an Applied Biosystems 7500 Real-time PCR System.
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bioRxiv - Immunology 2022Quote: ... RNA concentrations were determined using the Qubit high-sensitivity RNA assay and small RNA libraries were created using the Clontech SMARTer smRNA-Seq preparation kit according to manufacturer instructions (Takara). Small RNA inserts were further enriched using AMPure bead selection (Beckman Coulter ...
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bioRxiv - Microbiology 2022Quote: ... culture supernatants were collected from each well to measure lactate dehydrogenase (LDH) release by using an LDH cytotoxicity assay (Clontech) according to the manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2023Quote: ... but was tested in a directed two-hybrid assay in which cDNAs encoding ATI1 and ATI2 were cloned into the prey vector pGADT7 (Clontech) and co-transformed into PJ69-4A with the NcGNAGO1 bait plasmid ...
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bioRxiv - Cell Biology 2023Quote: ... pH 7.4) and the protein contents of the cell lysate were quantified using a BCA Protein Assay Kit (TAKARA, T9300A) following the manufacturer’s protocol (Song et al. ...
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bioRxiv - Synthetic Biology 2023Quote: ... SEAP activity in supernatant (25 μL) was measured using the Great EscAPe SEAP Chemiluminescence Assay kit 2.0 (Clontech, Fremonet, CA). The GLuc activity in supernatant (20 μL ...
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bioRxiv - Microbiology 2023Quote: ... culture supernatants were collected from each well to measure lactate dehydrogenase (LDH) release by using an LDH cytotoxicity assay (Clontech) according to the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2023Quote: ... Supernatants were collected and mixed with Laemmli buffer after measuring the protein concentration using a TaKaRa BCA Protein Assay Kit (TaKaRa). Samples were subjected to SDS-polyacrylamide gel electrophoresis and electrotransferred onto polyvinylidene difluoride membranes ...
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bioRxiv - Plant Biology 2024Quote: ... two-hybrid assay was conducted according to the manufacturer’s instructions for the Match-maker Gold Yeast Two-Hybrid System (Clontech, USA). To generate the bait construct BD-GhCPK28 ...
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bioRxiv - Molecular Biology 2024Quote: ... Final cDNA samples were used then used for quantitative real-time PCR assay by SYBR Green PCR Kit (RR820A, Takara). Quantitative primers were designed based on the gene sequences by Sangon Biotech ...
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bioRxiv - Plant Biology 2020Quote: ... The yeast one-hybrid (Y1H) assay was conducted using the Matchmaker™ Gold Yeast One-Hybrid Library Screening System kit (Cat. no. 630491, Clontech).
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bioRxiv - Plant Biology 2022Quote: ... The recombinant constructs was transformed into Y1H gold strain and Y1H assay was performed using Matchmaker® Gold Yeast One-hybrid screening system (Takara) following the manufacturer’s protocol ...
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bioRxiv - Genetics 2019Quote: Generated cDNAs were diluted to 1/5 and were used as template in a real time quantitative PCR assay using SYBR®Premix Ex TaqTM II (Tli RNaseH Plus) (Takara). All qRT-PCR reactions were performed in duplicate using Bio-Rad CFX-96 Connect system with the following conditions ...
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bioRxiv - Genetics 2021Quote: 0,1 ng of retrieved DNA in Cut&Run were used as template in a real time quantitative PCR assay using SYBR® Premix Ex TaqTM II (Tli RNaseH Plus) (Takara). All qPCR reactions were performed in duplicates using Bio-Rad CFX-96 Connect system with the following conditions ...
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bioRxiv - Plant Biology 2020Quote: ... For Yeast-Two-Hybrid assays HvRIPb and HvRIPb truncations were subcloned from the pGEM-T easy vector into pGADT7 and pGBKT7 plasmids (Clontech Laboratories) using the EcoRI and BamHI restriction sites ...
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bioRxiv - Plant Biology 2020Quote: ... The qRT-PCR assay was performed using 1/20 diluted cDNA as templates in the reactions containing SYBR® Premix Ex TaqTM II (TaKaRa). The qRT-PCR assay was conducted in triplicate in an ABI 7500 Fast Real-Time PCR System ...
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bioRxiv - Developmental Biology 2020Quote: ... TUNEL assay was performed to detect apoptotic cell death using the in situ Apoptosis Detection Kit (Takara Bio Inc., Shiga, Japan) according to the manufacturer’s instructions ...
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bioRxiv - Plant Biology 2019Quote: Y1H assays were carried out using the Matchmaker Gold yeast one hybrid Library Screening System (Biosciences Clontech, Palo Alto, CA, USA). The PRH1 promoter was inserted into the pAbAi vector ...
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bioRxiv - Plant Biology 2019Quote: ... pGBKT7-Pha21 and pGADT7-UBQ was co-transformed into AH109 yeast strain for yeast two-hybrid assay by using the Make Your Own “Mate & Plate” Library System (Takara Bio) following the user manual ...
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bioRxiv - Plant Biology 2021Quote: ... The Y2H assay was performed following the manual provided in the kit “Matchmaker Gold Yeast Two-Hybrid” (Clontech, Mountain View, USA). The list of VirE2 interacting clones has been given in Fig.S1.
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bioRxiv - Molecular Biology 2020Quote: Yeast-2-hybrid assays were performed with the plasmids and strains from the Matchmaker Gold Yeast Two-Hybrid System (Takara Bio) according to manufacturer’s protocol ...
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bioRxiv - Genomics 2021Quote: ... The four PCR assays were run on a thermal cycler using 100 ng DNA with PrimeStar GXL PCR mastermix (Takara Bio) according to the manufacturer’s instruction using 58 °C annealing temperature ...
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bioRxiv - Plant Biology 2023Quote: ... The recombinant constructs were introduced into Y1H gold strain and Y1H assay was performed using Matchmaker® Gold Yeast One-hybrid screening system (Takara) following the manufacturer’s protocol ...
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bioRxiv - Immunology 2021Quote: ... The transcripts of the DEMs and DEPs were analyzed and validated by qRT-PCR assays using the Mir-X miRNA qRT-PCR SYBR® Kit (TaKaRa, Japan) and the PrimeScriptTM RT reagent Kit (TaKaRa ...
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bioRxiv - Immunology 2021Quote: ... and the primers of the U6 snRNA used in the qRT-PCR assays were provided in the Mir-X miRNA qRT-PCR SYBR® Kit (Takara, Japan). The qRT-PCR data were analyzed by the 2−ΔΔCt method as described in Livak & Schmittgen (2001 ...