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Citations for Takara Bio :
1 - 50 of 366 citations for Rat Colony Assay Media since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Bioengineering 2023Quote: Single colonies were grown in 10 ml YPDA media (Takara) in 50 ml tubes for 24h ...
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bioRxiv - Genetics 2022Quote: ... Colonies were grown on media lacking histidine and leucine (DO Supplement -His/-Leu, Takara Bio) to select for the presence of both vectors ...
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bioRxiv - Genetics 2021Quote: ... Colony PCR was performed using Takara Taq polymerase (Clontech, TAKR001C). Plasmid DNA was isolated from cultured bacteria using QIAprep Spin Miniprep Kit (Qiagen ...
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bioRxiv - Microbiology 2021Quote: ... Positive colonies were selected on aureobasidin A (Takara, Shiga, Japan), and further confirmed via PCR ...
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bioRxiv - Physiology 2022Quote: ... Media lactate dehydrogenase (LDH) was assayed by enzymatic colorimetric assay (LDH Cytotoxicity Detection Kit, Takara Bio, cat# MK401).
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bioRxiv - Genomics 2022Quote: ... Individual colonies had their plasmids extracted using NucleoSpin Plasmid (Takara Bio) and Sanger sequenced to verify successful ligation with no sequence errors ...
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bioRxiv - Microbiology 2019Quote: ... After checking the colonies by PCR using EmeraldAmp MAX (Takara Bio), the selected cells were grown in DS medium with antibiotics ...
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bioRxiv - Molecular Biology 2019Quote: ... Colony PCR reaction contained 10 μl 2×La Taq Mix (Takara), 1 μl colony ...
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bioRxiv - Cell Biology 2021Quote: ... and subsequent colony sequencing of transformed Stellar™ Competent Cells (Takara, 636766). gRNA sequence ...
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bioRxiv - Plant Biology 2022Quote: ... followed by selection of transformants and testing of pair-wise interactions by growth complementation assays on nutrient selection media as described in the Matchmaker™ GAL4 Two-Hybrid System 3 manual (Clontech). To compensate for auto-activation of some constructs ...
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bioRxiv - Neuroscience 2023Quote: ... DNA from two selected colonies was amplified using LA Taq DNA polymerase (TaKaRa) and the region between the homology arms was sequenced ...
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bioRxiv - Developmental Biology 2022Quote: ... rat anti-E-Cadherin (TAKARA; M110), rat anti-Endomucin (Santa Cruz ...
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bioRxiv - Pathology 2022Quote: ... Rat anti Mouse OCN (Takara, M188); Rabbit anti Mouse MGST1 (Abcam ...
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bioRxiv - Cell Biology 2021Quote: ... Resulting colonies were then screened using the Guide-it Mutation Detection Kit (Clontech Laboratories, 631443). Knockout was further confirmed via western blot ...
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bioRxiv - Synthetic Biology 2023Quote: ... ∼¼ of a healthy uniform colony was suspended in complete synthetic medium (SCM) (Takara Bio USA). The cell concentration of each inoculum was measured by flow cytometry and then diluted to 1 cell/ul in an erlenmeyer flask ...
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bioRxiv - Plant Biology 2023Quote: ... Positive yeast colonies were selected and confirmed by using different synthetic drop-out (SD) (Takara) media prepared according to the manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2022Quote: ... BCA assays using BCA assay kit (TaKaRa) were performed before boiling the lysed cells or medium mixed with 1/3 volume of 4× sample buffer for 10-15 minutes at 90°C ...
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bioRxiv - Genetics 2023Quote: ... The media are from Clontech Laboratories ...
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bioRxiv - Cell Biology 2020Quote: ... gDNA of CFU colonies was extracted by suspending the cell in 1×PCR buffer (Takara, R050A) containing 0.2mg/ml proteinase K (Tiangen ...
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bioRxiv - Neuroscience 2023Quote: ... Rat Shank3 deletion constructs in pmRFP-N3 (Clontech) were described before (23) ...
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bioRxiv - Developmental Biology 2023Quote: ... rat anti-CDH1 (M108, Takara Biosciences, 1:500), rabbit anti-FOXA2 (Abcam ...
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bioRxiv - Microbiology 2019Quote: ... Outside-in colony PCR of a subset of hpn genes was performed with PrimeSTAR DNA polymerase (TaKaRa) using the following primers:
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bioRxiv - Cell Biology 2021Quote: ... Transformants were screened by colony PCR using the primer set JT37/JT38 and Sapphire polymerase (Takara Bio), and positive clones were selected for outgrowth ...
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bioRxiv - Cell Biology 2020Quote: ... Rat anti-E-cadherin mAb (ECCD2; M108, Takara Bio), mouse anti-α-tubulin mAb (14-4502-82 ...
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bioRxiv - Genomics 2022Quote: ... 20 individual bacterial colonies were separately picked from a dilution plating and miniprepped using Nucleospin Plasmid (Takara Bio), and the extracted plasmids were Sanger sequenced to verify the cloning quality of the library ...
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bioRxiv - Genetics 2021Quote: ... minimal SD base media (Takara Bio, 630411), and a composition of every essential amino acid except tryptophan (SD/-Trp ...
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bioRxiv - Plant Biology 2021Quote: ... The yeast growth media (YPD medium, Clontech), SD growth medium w/o Ade−His−Leu−Trp− (Himedia ...
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bioRxiv - Cell Biology 2020Quote: ... the media was replaced with NDiff227 (Takara) supplemented with 1 μM PD325901 (Stemgent) ...
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bioRxiv - Systems Biology 2022Quote: ... media supplemented with 10% FBS (Takara, 632180), and 1% Penicillin Streptomycin (Gibco ...
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bioRxiv - Systems Biology 2022Quote: ... media supplemented with 10% FBS (Takara, 632180) and 1% Penicillin Streptomycin Glutamine (Gibco ...
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bioRxiv - Plant Biology 2022Quote: ... All yeast media were purchased from Clontech.
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bioRxiv - Cell Biology 2024Quote: ... the media was replaced with NDiff227 (Takara) supplemented with 1 μM PD325901 (Stemgent) ...
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bioRxiv - Neuroscience 2019Quote: ... Primary antibodies used were: rat monoclonal mCherry (1:2000, Clontech); mouse monoclonal tyrosine hydroxylase (TH ...
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bioRxiv - Developmental Biology 2022Quote: ... anti-E-cadherin rat monoclonal antibody (Takara, M108, 1:500), anti-GFRα1 goat polyclonal antibody (R&D Systems ...
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bioRxiv - Physiology 2023Quote: ... and rat anti-E-cadherin (1:200, Takara Bio, M108). Next ...
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bioRxiv - Microbiology 2022Quote: ... The plasmids were extracted from all the colonies which grew on QDO/X/A agar plate using Easy Yeast Plasmid Isolation Kit (TaKaRa). PCR was carried out using the extracted plasmids as template ...
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bioRxiv - Microbiology 2024Quote: ... The deletion of each Stx2a phage in the CmR transformants was confirmed by colony PCR using EmeraldAmp MAX PCR Master Mix (TaKaRa) and specific primers ...
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bioRxiv - Bioengineering 2024Quote: ... Appropriate colonies were grown and the recombinant bacmid DNA was extracted using the NucleoBond Xtra Midi plasmid purification kit (TAKARA). The fifth instar silkworm larvae ...
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bioRxiv - Developmental Biology 2021Quote: ... 24 hour old pluripotent spheroids were subjected to CL media consisting of RHB Basal media (Takara/Clontech, cat. no. Y40000), 5% KSR (Thermo Fisher Scientific ...
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bioRxiv - Developmental Biology 2021Quote: ... 24 hour old pluripotent spheroids were subjected to CL media consisting of RHB Basal media (Takara/Clontech, cat. no. Y40000), 5% KSR (Thermo Fisher Scientific ...
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bioRxiv - Plant Biology 2021Quote: ... whereas selective media additionally lacked His (−4) (Clontech).
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bioRxiv - Synthetic Biology 2019Quote: ... media containing 0.1 μM rapamycin analog (Takara AP21967) or 0.1% ethanol as a control was added to cells ...
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bioRxiv - Developmental Biology 2021Quote: ... –Leu selective media (Clontech, 630417, Mountain View, USA) and shown as controls for growth ...
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bioRxiv - Cancer Biology 2021Quote: ... coated Primaria plates in RHB-A media (Takara) supplemented with growth factors EGF (Sigma ...
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bioRxiv - Biophysics 2020Quote: ... coli MG1655 genome by colony PCR and cloned into pR6K-PAmCherry-lox71-cm-lox66 using the In-Fusion® HD cloning kit (Clontech). Mutations were introduced using the QuikChange II Site-Directed Mutagenesis Kit (Agilent) ...
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bioRxiv - Bioengineering 2021Quote: ... To verify deletions or test for the presence of the CPOT plasmids colony PCR was performed using SapphireAmp fast PCR mix (TaKaRa Bio). For DNA construction ...
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bioRxiv - Genomics 2022Quote: ... 1.51 million bacterial colonies were collected and the plasmids were extracted with NucleoBond Xtra Maxi EF kit (Takara Bio USA #740424). To insert a TmU DNA fragment containing the tracRNA (E/F ...
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bioRxiv - Plant Biology 2023Quote: ... were used to co-transform yeast strain AH109 and colonies carrying both vectors were selected on SD medium without tryptophan (-W) and leucine (-L) (TaKaRa 630317) at 28°C ...
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bioRxiv - Neuroscience 2021Quote: ... the TUNEL assay was performed using the ApoAlert™ DNA Fragmentation Assay Kit (Clontech) to detect the presence of DNA fragmentation in frozen tissue sections ...
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bioRxiv - Molecular Biology 2021Quote: ... the media was switched to RHB-A (Takara, Y40001) and was subsequently changed every other day ...