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Citations for Takara Bio :
301 - 350 of 762 citations for Radiography Imaging system since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Genomics 2020Quote: ... CDT1 cDNA was further cloned into the Tet-On 3G inducible expression system (TaKaRa, 631168) with a flag tag at the N terminal and an EGFP tag at the C terminal by the In-Fusion HD Cloning system (TaKaRa ...
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bioRxiv - Plant Biology 2020Quote: ... and Lamin (LAM) were cloned into the DNA-binding domain vector pGBKT7 (Clontech Matchmaker System). The full-length open reading frame of NAA50 ...
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bioRxiv - Plant Biology 2020Quote: Y1H assay was performed using Matchmaker Gold Yeast One-Hybrid Library Screening System (Clontech, USA). The nucleotide sequences of promoter region of selected key defense genes having potential RAV1 binding motifs were retrieved by using online tool (https://bioinformatics.psb.ugent.be/plaza/) ...
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bioRxiv - Cell Biology 2019Quote: ... PCR products were inserted into the respective plasmids using the In-Fusion cloning system (Clontech), the sequence- and ligation-independent cloning (SLIC ...
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bioRxiv - Plant Biology 2020Quote: ... RT–qPCR analysis was performed using a Thermal Cycler Dice Real Time System TP800 (Takara). The expression levels of genes of interest were normalized relative to those of UBC21 ...
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bioRxiv - Plant Biology 2021Quote: ... Yeast two hybrid assays were performed using the Matchmaker Gold Yeast Two-Hybrid System (Clontech). The standard protocol given in the kit was followed for Y2H assay ...
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bioRxiv - Plant Biology 2021Quote: ... β‐galactosidase assay was performed according to the manufacturer’s protocol (Matchmaker Two‐Hybrid System; Takara Bio ...
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bioRxiv - Microbiology 2021Quote: ... were prepared by PCR and the In-Fusion cloning system (Takara Bio, Kusatsu, Shiga, Japan). The inserted DNA sequence of each vector was confirmed by direct DNA sequencing ...
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bioRxiv - Neuroscience 2020Quote: ... The cDNA was then amplified using the Advantage 2 PCR Enzyme System (Takara, Fremont, CA) for 6 cycles ...
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bioRxiv - Cancer Biology 2021Quote: High-throughput quantitative PCR (qPCR) was performed using the SmartChip system (Takara Bio, Kusatsu, JAPAN) on 47 oral melanoma samples to detect focal amplifications on tumor DNA on CFA 10 and CFA 30 ...
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bioRxiv - Plant Biology 2022Quote: ... Growth was determined as described in the Yeast Two-Hybrid System User Manual (Clontech, Japan).
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bioRxiv - Biophysics 2022Quote: ... This plasmid expresses the PR isoform-B under a tetracycline controllable promoter (TetOff system, Clontech). To perform the SPT experiments ...
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bioRxiv - Cell Biology 2022Quote: Yeast two-hybrid analysis was performed with the Matchmaker Gold Yeast Two-Hybrid System (Takara). Y2H Gold and Y187 yeast strains were transformed with bait (pGBKT7 plasmids and derivatives ...
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bioRxiv - Cell Biology 2022Quote: Yeast-two-hybrid analysis was performed with the MATCHMAKER GAL4 Two-Hybrid System 3 (Clontech) essentially as described (39) ...
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bioRxiv - Plant Biology 2021Quote: ... viral RNA was amplified using a Thermal Cycler Dice Real Time System (TaKaRa, Shiga, Japan) with SYBR Premix Ex Taq II (TaKaRa) ...
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bioRxiv - Microbiology 2021Quote: ... PCR products were treated with DpnI and cloned using the In-Fusion cloning system (Takara). Following sequence confirmation ...
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bioRxiv - Microbiology 2021Quote: ... PCR products were cloned into the linearized pGE190 using the In-Fusion cloning system (Takara). The oligonucleotide templates had a “Y” (mixed base “C” or “T” ...
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bioRxiv - Plant Biology 2021Quote: Yeast two-hybrid assays were carried out using the Matchmaker Two-Hybrid System Kit (Clontech). The AtCGL160 CDS without the signal peptide (see Supplemental Table S2 for primer information ...
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bioRxiv - Cancer Biology 2021Quote: ... using the primers listed in Table 1 with the In-Fusion HD cloning system (Takara). To silence GSK3B expression ...
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bioRxiv - Genetics 2019Quote: The yeast two-hybrid assays were performed using the Matchmaker Yeast Two-Hybrid System (Clontech). Various fragments from OsLUGLWT and OsLUGLkkx were cloned into pGBKT7 ...
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bioRxiv - Plant Biology 2020Quote: Yeast two-hybrid assays were performed by the Matchmarker GAL4-based two-hybrid System (Clontech). Constructs were co-transformed into the yeast strain AH109 by the lithium acetate method (Gietz & Woods ...
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bioRxiv - Cell Biology 2020Quote: ... 293FT packaging cells were transfected with lentiviral cDNA using Lenti-X packaging system (631276; Takara). Cultured media containing lentiviral particles was harvested after 72h and used to infect 3T3-L1 preadipocytes ...
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bioRxiv - Plant Biology 2020Quote: ... Yeast transformation was performed according to the Yeastmaker Yeast Transformation System 2 User Manual (Clontech). The primers used are listed in Supplemental Table S2.
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bioRxiv - Plant Biology 2020Quote: ... Yeast transformation was performed according to the Yeastmaker Yeast Transformation System 2 User Manual (Clontech). More than 1 × 106 yeast clones were screened in synthetic defined (SD ...
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bioRxiv - Pharmacology and Toxicology 2020Quote: ... Expression of cytokines was quantified using a Thermal Cycler Dice Real Time System (Takara Bio) with SYBR Premix ExTaq II (Takara) ...
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bioRxiv - Pharmacology and Toxicology 2020Quote: ... Domain swapped HsPLZF was constructed by inverse PCR and the In-Fusion system (Takara Bio). Deletion mutation and amino acid mutation of each protein was performed by inverse PCR.
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bioRxiv - Cell Biology 2019Quote: ... T-REx™-293 cells were purchased from Thermo Fisher Scientific and cultured in MEM GlutaMAX™ supplemented with 10% Tet system approved FBS (Clontech) and 5 µg/ml blasticidin ...
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bioRxiv - Genomics 2021Quote: ... These cells were cultured using Cellartis DEF-CS 100 Culture System (DEF-CS medium; Takara) under feeder-free condition ...
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bioRxiv - Genomics 2021Quote: ... and enriched using SMARTer Apollo NGS library prep system (Takara Bio USA, Mountain View, CA). Libraries were prepared using NEBNext Ultra II Directional RNA Library Prep Kit (New England BioLabs) ...
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bioRxiv - Biochemistry 2021Quote: ... Ligation independent cloning was performed with the In-Fusion HD Cloning Plus system (Clontech #638910). Plasmid DNA was prepared with the QIAprep Spin Miniprep (QIAGEN #27106) ...
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Myosin VI regulates ciliogenesis by promoting the turnover of the centrosomal/satellite protein OFD1bioRxiv - Cell Biology 2021Quote: ... pLVX-GFP-OFD1 lentiviral construct was generated using the Infusion HD cloning system (Takara Clontech) following manufacturer’s instructions ...
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Myosin VI regulates ciliogenesis by promoting the turnover of the centrosomal/satellite protein OFD1bioRxiv - Cell Biology 2021Quote: ... pLVX-GFP-OFD1 lentiviral construct was generated using the Infusion HD cloning system (Takara Clontech) following manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2022Quote: The parental MCF10A cells were stably transduced with tetracycline-OFF-transactivator system (tet-OFF; Clontech) following a previously described protocol (pubmed.ncbi.nlm.nih.gov/27815877/) ...
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bioRxiv - Genetics 2022Quote: Y2H were performed using the Gold Yeast Two-Hybrid System according to manufacturer’s manual (Clontech). CDSs from different genes with combination of pGADT7 and pGBKT7 were co-transformed into the yeast strain AH109 ...
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bioRxiv - Genetics 2022Quote: ... and then yeast two-hybrid assays were performed in a Gal4-based system from Clontech in a diploid strain by mating as previously described (Rossignol et al ...
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bioRxiv - Plant Biology 2022Quote: Nuclear two-hybrid interaction assays were performed using the MATCHMAKER GAL4 two-hybrid system (Clontech). The CWLP coding region without signal peptide (SP ...
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bioRxiv - Plant Biology 2022Quote: We used the GAL4-based Matchmaker Gold Yeast 2-Hybrid System (Clontech, Mountain View, California) for all Y2H and Y3H assays ...
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bioRxiv - Molecular Biology 2022Quote: Stable clones were grown in medium containing Tet system-approved fetal bovine serum (TaKaRa Bio), puromycin ...
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bioRxiv - Plant Biology 2022Quote: ... The Y2H assays were carried out in the Matchmaker Gold Yeast Two-Hybrid System (Clontech). The pairs of corresponding recombinant constructs fused with pGBKT7 BD and pGADT7 AD were co-transformed into the Y2H Gold yeast competent cells ...
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bioRxiv - Plant Biology 2023Quote: Coding sequences were cloned in the GAL4 system Gateway® vectors (pGADT7 and pGBKT7; Clontech) using primers listed in Dataset S1 ...
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bioRxiv - Immunology 2023Quote: ... inducible cell lines were generated using the Tet-On Advanced Inducible Expression System (Clontech Laboratories) according to the manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2022Quote: ... PCR was performed using the PrimeSTAR Max DNA polymerase system (Takara Bio Inc., Shiga, Japan), with annealing at 53 °C (c.1018G>A ...
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bioRxiv - Plant Biology 2023Quote: ... and Y1H analysis was performed using Matchmaker® Gold Yeast One-hybrid screening system (Takara) according to the manufacturer’s protocol ...
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bioRxiv - Developmental Biology 2022Quote: ... cells were cultured using Cellartis DEF-CS 500 culture system (Takara Bio, cat. no. Y30012).
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bioRxiv - Developmental Biology 2023Quote: ... The ORF of human KIF23 was cloned within pCAX vector using In-Fusion system (TaKaRa). The disease associated c.755T>A mutation was introduced to human KIF23 cDNA by the PCR-based mutagenesis as described (Xia et al. ...
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bioRxiv - Cancer Biology 2023Quote: We used the Lenti-X™ Tet-On® Advanced Inducible Expression System (Clontech, 632162) to produce the lentivirus vectors and transfect cells ...
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bioRxiv - Plant Biology 2023Quote: Yeast two-hybrid screening was performed using the Matchmaker Gold Yeast Two-Hybrid System (Takara). The cDNA fragments corresponding to CEPDL2 and GrxS5 were subcloned into the pGBKT7 vector ...
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bioRxiv - Microbiology 2023Quote: ... these four fragments were fused together using the In-Fusion system (Takara Bio, Inc., Japan).
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bioRxiv - Molecular Biology 2023Quote: ... A quantitative (q)PCR was performed on a Thermal Cycler Dice Real Time System (Takara) with the use of Power SYBR Green Master Mix (A25742 ...
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bioRxiv - Microbiology 2023Quote: Y2H assays were performed according to Yeastmaker™ Yeast Transformation System 2 User Manual (Clontech). The coding sequences corresponding to HCPro1 ...