Labshake search
Citations for Takara Bio :
551 - 600 of 762 citations for Radiography Imaging system since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Molecular Biology 2021Quote: ... qRT-PCR was performed using SYBR Premix Ex TaqII (Tli RNaseH Plus) and the Thermal Cycler Dice Real Time System (TaKaRa). The results were analyzed based on standard curves from a serial dilution of cDNA ...
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bioRxiv - Cancer Biology 2020Quote: ... Human melanoma cell lines and Hema-LP were overlaid with viral particles diluted in DMEM/1x Glutamax with 10% TET System Approved FBS (Clontech) supplemented with 5 μg/ml polybrene (Sigma-Aldrich) ...
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bioRxiv - Systems Biology 2020Quote: ... The cell lines were cultured in IMDM medium (Quality Biological) supplemented with 10% (v/v) Tet System Approved fetal calf serum (Takara Bio/previously Clontech), 100 U/mL penicillin ...
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bioRxiv - Plant Biology 2021Quote: ... MEL1 and lacZ) under distinct GAL4 upstream activating sequences as described in Matchmaker GAL4 Two-Hybrid System 3 & Libraries User Manual (Clontech). The transformants were grown on synthetic dropout (SD ...
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bioRxiv - Plant Biology 2020Quote: The yeast two hybrid (YTH) experiments were performed in three replicates using the Matchmaker Gal4-based two-hybrid system (Clontech) and the Saccharomyces cerevisiae strain Y2HGold as described in (11) ...
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bioRxiv - Genomics 2019Quote: SiGRF1 protein interactions were investigated by screening a foxtail millet cDNA library in yeast with the Matchmaker Two-Hybrid System 3 (Clontech). SiGRF1 cDNA without the termination codon was cloned with EcoR1 technology in pGBKT7 ...
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bioRxiv - Cell Biology 2019Quote: ... and lentiviral Tre3G-Cas9 and Tet3G particles were produced in HEK293T cells using the Lenti-X HT packaging system (Clontech). Transduced cells were selected with 10 μg/mL puromycin and 400 μg/mL G418 ...
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bioRxiv - Neuroscience 2019Quote: ... Israel) into the pEGFP-N2 vector (restriction sites BamHI and NotI, removing EGFP) using the Infusion cloning system from Clontech. The FLAG sequence was then inserted downstream of the signaling peptide (amino acids 1-27 ...
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bioRxiv - Molecular Biology 2020Quote: PJ69-4a strain of Saccharomyces cerevisiae was used for the expression of proteins of interest using the Matchmaker Gal4-based yeast two hybrid system (Clontech). Tested bait/prey combinations were co-transformed into PJ69-4a and cultivated at 28°C ...
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bioRxiv - Genomics 2021Quote: ... the target site of the CRISPR/Cas9 system on dnmt3aa gene was amplified using TaKaRa Ex Taq® (Takara Bio) with the primers listed in Supplementary Table S2 ...
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bioRxiv - Plant Biology 2021Quote: ... was then used for RT-qPCR using the primers shown in Supplementary Table S1 with THUNDERBIRD SYBR qPCR Mix (TOYOBO) on a Thermal Cycler Dice Real Time System (TaKaRa). P ...
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bioRxiv - Genetics 2021Quote: ... Quantitative PCR was performed using KAPA SYBR Fast qPCR Kits (Nippon Genetics, Japan) on Dice Real Time System Single Thermal Cycler (Takara) or CFX96 Real- Time System (BioRad ...
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bioRxiv - Molecular Biology 2019Quote: ... The specific PCR products were gel purified by using the DNA Gel Extraction Kit (Axygen, USA) and cloned to the pMD-18 vector system (Takara), and then sequenced by the Shanghai Sangon Company.
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Osh6 requires Ist2 for localization to the ER-PM contacts and efficient phosphatidylserine transportbioRxiv - Cell Biology 2020Quote: For testing protein-protein interactions Matchmaker™ GAL4 Two-Hybrid System 3 was used according to the manufacturer’s instructions (Clontech). Briefly ...
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bioRxiv - Plant Biology 2020Quote: ... bait plasmids and prey plasmids were co-transformed into yeast strain AH109 according to the manufacturer’s protocol of GAL4-based yeast two-hybrid system (Clontech, USA). Then ...
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bioRxiv - Molecular Biology 2021Quote: ... The purified ds CDNA was finally co-transformed with pGAD-T7 Rec into competent Y187 using Yeastmaker yeast transformation system (Takara, Clontech) and plated on SD-Leucine agar media ...
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bioRxiv - Molecular Biology 2021Quote: ... Recombinant OmpA-pGBKT7 and Flg-pGBKT7 were finally transformed into Y2H Gold yeast strain separately using Yeastmaker yeast transformation system (Takara, Clontech).
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bioRxiv - Molecular Biology 2021Quote: ... First strand cDNA was synthesized with 3.6 μg of total RNA using Make your own ‘mate & plate’ library system (Takara, Clontech) according to the manufacturer’s instruction ...
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bioRxiv - Neuroscience 2020Quote: ... qRT-PCR was performed using TOYOBO THUNDERBIRD SYBR qPCR Mix on a Thermal Cycler Dice Real Time System (Takara Bio). The average threshold cycle value (CT ...
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bioRxiv - Microbiology 2022Quote: ... A tomato Mate & Plate library was constructed and used for screening with the Matchmaker Gold Yeast Two-Hybrid System (Clontech) according to the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2022Quote: We used the In-Fusion™ (IF) HD cloning system online primer design website (Takara Bio USA, Mountain View, CA) to create primer sets (Table S1 ...
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bioRxiv - Molecular Biology 2022Quote: ... pCAGGS was digested by SpeI and BamHI and assembled with the PCR products by an In-Fusion HD cloning system (Clontech). After transformation into DH5α ...
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bioRxiv - Biophysics 2022Quote: ... The cell culture medium was replaced with pre-warmed DMEM containing 10% Tet system approved FBS (Tet-free medium, TaKaRa) and 30 μL of DNA-lipid complex was added to each well ...
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bioRxiv - Developmental Biology 2022Quote: ... 2017) into the 3’ end of AQP1 in pBS-AQP1 (described above) using the In-Fusion Cloning system (Takara Bio) with the following primers ...
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bioRxiv - Genomics 2022Quote: Genomic DNA was used as a template for quantitative PCR analysis on a Thermal Cycler Dice Real Time System (TaKaRa) using KAPA SYBR Fast qPCR Kit (KAPA Biosystems ...
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bioRxiv - Systems Biology 2022Quote: ... with 1 μg of total RNA as input for the SMARTer Apollo NGS library prep system (Takara, Mountain View, CA). The library for RNA-seq was prepared by using NEBNext Ultra II Directional RNA Library Prep kit (New England BioLabs ...
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bioRxiv - Plant Biology 2022Quote: ... which was conducted with the “Make Your Own “Mate & Plate™” Library System” according to the manufacturer’s instructions with minor modifications (Clontech). Oligo-dT primers provided in the kit were used for first-strand cDNA synthesis with 2 µg total RNA as input ...
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bioRxiv - Neuroscience 2022Quote: ... These plasmids were obtained by cloning cDNA extracted from mouse cerebellum in pAAV-MCS Expression Vector with In-Fusion Cloning system (Takara). 96h after transfection ...
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bioRxiv - Plant Biology 2022Quote: ... The SlBZR1 CDS was introduced into the pGBKT7 vector and then used as bait to screen the cDNA library based on the Matchmaker Gold Yeast Two-Hybrid System (Clontech). Yeast transformants were exhaustively selected on SD/-Ade/-His/-Leu/-Trp/X-α-Gal medium ...
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bioRxiv - Plant Biology 2022Quote: ... Quantitative real-time PCR (qRT-PCR) was performed on three biological replicates using the Thermal Cycler Dice Real Time System TP-760 (Takara) with the Kapa SYBR fast universal qPCR kit (Kapa Biosystems) ...
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bioRxiv - Neuroscience 2023Quote: ... pTRE3G-HA signal-Flag-GPCRs-P2A-mCherry-reverse-PGK-TetOn3G was made of Tet-ON 3G inducible expression system (Clontech) and PRESTO-Tango GPCR Kit (Kroeze et al. ...
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bioRxiv - Microbiology 2023Quote: A yeast screen for human cellular interacting proteins of pUL136 was performed using the Matchmaker Gold Yeast Two-Hybrid System (Clontech) and the Mate and Plate Universal Human Library (Clontech) ...
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FTSH3 facilitates complex I degradation through a direct interaction with the complex I subunit PSSTbioRxiv - Plant Biology 2023Quote: ... Mating was carried out in a 96-well plate according to manufacturer’s instructions (Matchmaker GAL4 Two Hybrid System (Clontech/TakaraBio). Mated diploid strains were serially diluted and plated on - Leu–Trp double dropout (DDO ...
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bioRxiv - Plant Biology 2023Quote: ... pYES2-SiHDA9 and empty pYES2 vector (EV) were transformed individually into Saccharomyces cerevisiae strain W303 using Yeastmaker Yeast Transformation System (Clontech). The transformed cells were screened on SD/-ura medium with 2% (w/v ...
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bioRxiv - Plant Biology 2023Quote: ... The cDNA library was prepared from rice flowers and ligated to the pGADT7-Rec vector using the Make Your Own “Mate and Plate” Library System (Takara). A second cDNA library was prepared from rice leaves under salt stress ...
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bioRxiv - Molecular Biology 2022Quote: ... was derived from foreskin-fibroblasts using the Stemgent mRNA Reprogramming Kit (Stemgent) as described previously (40) and maintained in a feeder-free culturing system Cellartis DEF-CS 500 (Takara). The inducible SpCas9 cell line was generated as described earlier (41).
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bioRxiv - Molecular Biology 2023Quote: ... and HEK293-FT cells were used to prepare lentiviral particles according to the instructions in the Lenti-XTM Lentiviral Expression System Manual (Clontech). Doxycycline-inducible ZDHHC20 expressing HEK293T cells were prepared by transduction of 2.5 X 105 low-passage cells with lentivirus ...
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bioRxiv - Cell Biology 2023Quote: ... was amplified from Tak-1 genomic DNA by PCR using KOD plus with the primers in Table S1 and cloned into the unique NotI site of pENTR/D-TOPO-MpARK by In-Fusion system (Takara) to generate pENTR/D-TOPO-MpARKpro:MpARK ...
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bioRxiv - Plant Biology 2023Quote: ... the chimeric H5Indo gene was reamplified by PCR and then introduced in the T-DNA region of a pCAMBIA binary vector previously linearized with restriction enzymes SacII and StuI using the In-Fusion cloning system (Clontech). The same methods and PDI signal peptide were used to clone antibody chain genes ...
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bioRxiv - Genetics 2023Quote: ... the sequences corresponding to Flag tags were replaced by those encoding for Myc tags using the In-Fusion PCR cloning system (Takara) according to the kit’s guidelines ...
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bioRxiv - Plant Biology 2023Quote: ... the chimeric H5Indo gene was reamplified by PCR and then introduced in the T-DNA region of a pCAMBIA binary vector previously linearized with restriction enzymes SacII and StuI using the In-Fusion cloning system (Clontech). Expression of H5Indo was driven by a 2X35S promoter from the cauliflower mosaic virus (CaMV) ...
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bioRxiv - Cell Biology 2023Quote: ... HEK293T packaging cells were transfected with the required lentiviral cDNA constructs using Lenti-X packaging system following the manufactures protocol (631276, Takara). SKM cells stably expressing HA-GLUT4-GFP (SKM-GLUT4 cells ...
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bioRxiv - Neuroscience 2023Quote: Transgene constructs that simultaneously encode FingR targeting PSD95 and membrane markers of neuronal morphology were made using the In-Fusion HD Cloning System (Clontech). First ...
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bioRxiv - Plant Biology 2023Quote: ... and was cloned into the AscI site of the pENTR/D-TOPO entry vector containing full length MpNEK1 CDS (Otani et al., 2018) by In-fusion system (Takara). The resulting vector pENTR/D-TOPO-MpNEK1-mCitrine was subjected to LR reaction to transfer MpNEK1-Citrine fusion into the Gateway binary vector pMpGWB144 (MpEF1α pro:XVE >>LexA operator:Gateway cassette).
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bioRxiv - Cancer Biology 2022Quote: Doxycycline inducible RARα expressing phyllodes tumor patient-derived cells (PT024 RARα) were established with Tet-On 3G system from Takara. Coding sequence of full-length WT RARα were cloned into a PLVX-Tre3G-IRES-GFP response vector ...
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bioRxiv - Cell Biology 2023Quote: ... Separate populations were stably-transfected to express MLCK1-EGFP constitutively and mCherry-Ig3 inducibly via a Tet-on3G system (Clontech). Cells were maintained in high-glucose Dulbecco’s modified Eagle’s medium (Life Technologies ...
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bioRxiv - Biochemistry 2023Quote: ... Constructs used to test protein-protein interactions were co-transformed into the yeast Saccharomyces cerevisiae strain AH109 using the Matchmaker Gold yeast two-hybrid system (Clontech). Empty pDEST-GBKT7 and pDEST-GADKT7 vectors were used as negative control ...
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bioRxiv - Biochemistry 2023Quote: The full-length SKP1-FBXO22 fusion protein with a 3xGGGS-linker was cloned into a pNic-Bio2 vector that encodes for an N-terminal His-tag as well as a C-terminal Avi-tag (GenBank: JF912191) using an In-Fusion HD Cloning System kit (Takara), following the manufacturer’s instructions ...
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bioRxiv - Microbiology 2023Quote: ... the CPER products (25 μl) were transfected into VeroE6/TMPRSS2 cells using TransIT-X2 Dynamic Delivery System (Takara, Cat# MIR6003) according to our previous report22 ...
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bioRxiv - Cell Biology 2023Quote: ... 91 The plasmids pME-rtTA and p3E-TRE were made by inserting the Tet-On fragments from the Tet-On system (Clontech) in the appropriate Tol2 entry vectors ...