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Citations for Takara Bio :
251 - 300 of 4784 citations for QuantiChrom β Glucosidase Assay Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biochemistry 2022Quote: ... by In-fusion cloning kit (Takara). pIZ-Siwi-D670A and pIZ-BmAgo3-D697A mutants were generated by site-directed mutagenesis.
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bioRxiv - Biochemistry 2022Quote: ... by In-fusion cloning kit (Takara). For Gtsf1L ...
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bioRxiv - Developmental Biology 2022Quote: ... RT-PCR kit (Takara, Tokyo, Japan) was used to construct reverse transcription to collect cDNA ...
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bioRxiv - Neuroscience 2022Quote: ... using the Infusion cloning kit (Takara). Ligations were transformed in DH5α cells plated on LB+ Kanamycin plates ...
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bioRxiv - Microbiology 2023Quote: ... PrimeScript™ RT Reagent Kit (TAKARA) was used to generate cDNA and FastStart Essential DNA Green Master (Roche ...
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bioRxiv - Neuroscience 2023Quote: ... In-Fusion HD Cloning Kit (Clontech), and primers which included NNK codons ...
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bioRxiv - Genomics 2023Quote: ... SMART-Seq v4 kit (Clontech, 634893) was used for the reverse transcription and cDNA amplification according to the manufacturer’s specifications ...
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bioRxiv - Cell Biology 2023Quote: ... using In-Fusion Cloning kit (Takara).
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bioRxiv - Immunology 2023Quote: Using PrimeScript RT reagent Kit (TaKaRa), 0.5 µg of total RNAs were reverse transcribed into complementary DNA ...
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bioRxiv - Cell Biology 2022Quote: ... with the SYBR premeraseTaq kit (TAKARA). Amplified mRNA levels were normalized to 18s mRNA ...
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bioRxiv - Molecular Biology 2023Quote: ... retrotranscribed using PrimeScript Reagent Kit (Takara) and amplified by RT-qPCR using PowerUp SYBR-Green MasterMix (Thermo Fisher Scientific) ...
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bioRxiv - Molecular Biology 2023Quote: ... SMARTer PCR cDNA synthesis kit (Clontech) was used according to the manufacturer’s specifications ...
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bioRxiv - Neuroscience 2023Quote: ... using Infusion kit (Takara catalog # 638949). For some enhancers we instead cloned into a next generation reporter vector backbone that includes a SYFP2-P2A-3xFLAG-H2B reporter for detection of cytosolic SYFP2 and nuclear FLAG for simultaneous expression analysis and snRNA-seq ...
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bioRxiv - Molecular Biology 2023Quote: The Universal GenomeWalker 2.0 kit (Clontech Laboratories Inc. ...
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bioRxiv - Molecular Biology 2023Quote: ... In-Fusion HD Cloning Kit (Clontech) was used for this reaction.
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bioRxiv - Neuroscience 2023Quote: ... and AAV titration kit (TaKaRa/Clontech). The purity of viruses was routinely checked by silver staining (Pierce ...
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bioRxiv - Neuroscience 2023Quote: ... and AAV titration kit (TaKaRa/Clontech). The purity of viruses was routinely checked by silver staining (Pierce ...
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bioRxiv - Molecular Biology 2023Quote: ... by In-fusion cloning kit (Takara).
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bioRxiv - Molecular Biology 2023Quote: ... by In-fusion cloning kit (Takara).
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bioRxiv - Cell Biology 2023Quote: ... using In-Fusion cloning kit (Takara). We verified the sequences of both constructs pHR-[SFFV/FLAG::TurboID] and pHR-[SFFV/FLAG::TurboID::SQSTM1] using Sanger sequencing ...
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bioRxiv - Genetics 2023Quote: ... PrimeScript RT Reagent Kit (Takara, RR037) was used for cDNA synthesis ...
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bioRxiv - Plant Biology 2019Quote: For Yeast-two-Hybrid (Y2H) assays coding sequences were cloned into pDONR207 and recombined into the pGDAT7 and pGBKT7 (Clontech). Using the co-transformation techniques41 these constructs were transformed into the AH109 strain (Clontech).
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bioRxiv - Developmental Biology 2021Quote: Two-hybrid screening and assays were performed as described in the Matchmaker™ Pretransformed Libraries User Manual (Takara PT3183-1) using a Drosophila Mate & Plate™ Library - Universal Drosophila (Normalized A+ RNA isolated from embryo (~20 hr) ...
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bioRxiv - Molecular Biology 2021Quote: Nested translocation PCR assays was performed from genomic DNA as previously described (29) using either LA Taq DNA polymerase (Takara) or Flex HS polymerase (NEB) ...
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bioRxiv - Plant Biology 2020Quote: ... Plasmids for the PARN activity assay were constructed by inserting the coding sequence of RRD1 or human PARN (hPARN) into the pHAT vector (Clontech). The hPARN sequence was derived from the GNP Human cDNA clone IRAK071M01 (RIKEN BioResource Research Center) ...
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bioRxiv - Plant Biology 2021Quote: Yeast two-hybrid assays were performed as described (Cesari et al., 2013) using the Matchmaker Gold Yeast Two-hybrid system (Clontech).
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bioRxiv - Immunology 2022Quote: Lytic cell death was determined by measuring LDH release from cell-free supernatants using a colorimetric assay (Promega or Takara) according to the manufacturer’s protocol ...
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bioRxiv - Microbiology 2022Quote: ... Virus production was quantified by determining the amount of the Gag p24 protein using an enzyme-linked immunosorbent (ELISA) assay (Innogenetics or TaKaRa). For production of HIV-1 Env-pseudotyped viruses in Jurkat cells ...
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bioRxiv - Molecular Biology 2020Quote: ... Confirmatory “1-to-1” pairwise assays for selected interactants were performed with the MatchMaker Two-Hybrid System 3 (Clontech Inc.)
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bioRxiv - Genomics 2020Quote: Yeast one-hybridization assay was performed using the Matchmaker® Gold Yeast One-Hybrid System (Clontech, Palo Alto, CA, USA). The promoter sequence (upstream 2kb from the start codon ...
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bioRxiv - Microbiology 2022Quote: ... culture supernatants were collected from each well to measure lactate dehydrogenase (LDH) release by using an LDH cytotoxicity assay (Clontech) according to the manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2023Quote: ... but was tested in a directed two-hybrid assay in which cDNAs encoding ATI1 and ATI2 were cloned into the prey vector pGADT7 (Clontech) and co-transformed into PJ69-4A with the NcGNAGO1 bait plasmid ...
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bioRxiv - Microbiology 2023Quote: ... culture supernatants were collected from each well to measure lactate dehydrogenase (LDH) release by using an LDH cytotoxicity assay (Clontech) according to the manufacturer’s instructions ...
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bioRxiv - Plant Biology 2024Quote: ... two-hybrid assay was conducted according to the manufacturer’s instructions for the Match-maker Gold Yeast Two-Hybrid System (Clontech, USA). To generate the bait construct BD-GhCPK28 ...
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bioRxiv - Immunology 2019Quote: ... Viral titers were measured by a kit (Lenti-X p24 Rapid titer kit, #632200. Takara, Kusatsu, Japan). To suppress Mul1 expression ...
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bioRxiv - Neuroscience 2020Quote: ... and reverse transcribed using complementary DNA (cDNA) synthesis kit (PrimeScript 1st strand cDNA Synthesis Kit, Takara Bio). The synthesized cDNA was subjected to qPCR using KAPA SYBR (KAPABiosystems ...
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bioRxiv - Neuroscience 2020Quote: ... Viral titer was determined using a qPCR Lentivirus Titration Kit (Lenti-X, qRT-PCR Titration Kit, Takara). For smaller scale virus preparation ...
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bioRxiv - Neuroscience 2020Quote: ... in a 100 mm dish using a calcium phosphate transfection kit (CalPhos Mammalian Transfection kit, Clontech, 631312). Two days post-transfection ...
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bioRxiv - Synthetic Biology 2022Quote: ... The kit used for qPCR experiments was PrimeScript™ II 1st Strand cDNA Synthesis Kit (TaKaRa, Japan), HiScriptII 1st Strand cDNA Synthesis Kit (Vazyme Biotech Co. ...
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bioRxiv - Plant Biology 2022Quote: ... The recombinant constructs was transformed into Y1H gold strain and Y1H assay was performed using Matchmaker® Gold Yeast One-hybrid screening system (Takara) following the manufacturer’s protocol ...
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bioRxiv - Genetics 2019Quote: Generated cDNAs were diluted to 1/5 and were used as template in a real time quantitative PCR assay using SYBR®Premix Ex TaqTM II (Tli RNaseH Plus) (Takara). All qRT-PCR reactions were performed in duplicate using Bio-Rad CFX-96 Connect system with the following conditions ...
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bioRxiv - Genetics 2021Quote: 0,1 ng of retrieved DNA in Cut&Run were used as template in a real time quantitative PCR assay using SYBR® Premix Ex TaqTM II (Tli RNaseH Plus) (Takara). All qPCR reactions were performed in duplicates using Bio-Rad CFX-96 Connect system with the following conditions ...
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bioRxiv - Plant Biology 2020Quote: ... For Yeast-Two-Hybrid assays HvRIPb and HvRIPb truncations were subcloned from the pGEM-T easy vector into pGADT7 and pGBKT7 plasmids (Clontech Laboratories) using the EcoRI and BamHI restriction sites ...
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bioRxiv - Plant Biology 2020Quote: ... The qRT-PCR assay was performed using 1/20 diluted cDNA as templates in the reactions containing SYBR® Premix Ex TaqTM II (TaKaRa). The qRT-PCR assay was conducted in triplicate in an ABI 7500 Fast Real-Time PCR System ...
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bioRxiv - Plant Biology 2019Quote: Y1H assays were carried out using the Matchmaker Gold yeast one hybrid Library Screening System (Biosciences Clontech, Palo Alto, CA, USA). The PRH1 promoter was inserted into the pAbAi vector ...
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bioRxiv - Plant Biology 2019Quote: ... pGBKT7-Pha21 and pGADT7-UBQ was co-transformed into AH109 yeast strain for yeast two-hybrid assay by using the Make Your Own “Mate & Plate” Library System (Takara Bio) following the user manual ...
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bioRxiv - Molecular Biology 2020Quote: Yeast-2-hybrid assays were performed with the plasmids and strains from the Matchmaker Gold Yeast Two-Hybrid System (Takara Bio) according to manufacturer’s protocol ...
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bioRxiv - Genomics 2021Quote: ... The four PCR assays were run on a thermal cycler using 100 ng DNA with PrimeStar GXL PCR mastermix (Takara Bio) according to the manufacturer’s instruction using 58 °C annealing temperature ...
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bioRxiv - Plant Biology 2022Quote: ... followed by selection of transformants and testing of pair-wise interactions by growth complementation assays on nutrient selection media as described in the Matchmaker™ GAL4 Two-Hybrid System 3 manual (Clontech). To compensate for auto-activation of some constructs ...
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bioRxiv - Plant Biology 2023Quote: ... The recombinant constructs were introduced into Y1H gold strain and Y1H assay was performed using Matchmaker® Gold Yeast One-hybrid screening system (Takara) following the manufacturer’s protocol ...