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Citations for Takara Bio :
101 - 150 of 4784 citations for QuantiChrom β Glucosidase Assay Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2023Quote: ... which was measured using a real-time PCR assay with a SARS-CoV-2 direct detection RT‒qPCR kit (Takara Bio, Siga, Japan). The IC50 was calculated by IC50 Calculator (https://www.aatbio.com/tools/ic50-calculator ...
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bioRxiv - Genomics 2019Quote: ... and the expression of IFN-β and IL-6 were measured by quantitative PCR (TAKARA, RR820A). The sequences of the primers were listed in the Supplementary Table 4.
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bioRxiv - Cell Biology 2023Quote: ... A filter-based β-galactosidase assay and liquid culture assay using ONPG were performed in three replicates as described in the Yeast protocols handbook (Clontech). As a positive control for the study ...
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bioRxiv - Biochemistry 2020Quote: ... Protein concentration was determined by Bradford assay (TAKARA). Nickel affinity purification of 10His-SUMO1T95R conjugates was carried out with Ni-NTA agarose beads (Qiagen) ...
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bioRxiv - Microbiology 2020Quote: ... For cell death measurement by LDH assay (Takara), culture supernatants were clarified with a 5 minute spin at 400×g ...
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bioRxiv - Microbiology 2019Quote: ... the WST-1 Cell Proliferation Assay System (TaKaRa) was used following the manufacturer’s recommendation ...
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bioRxiv - Cell Biology 2020Quote: ... transformed with pGEX-4T-2-Bbs5-WT in the presence of 0.1 mM isopropyl-β-D-thiogalactopyranoside (Takara). The proteins were purified with the glutathione-Sepharose 4B protein chromatography purification kit (GE Healthcare) ...
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bioRxiv - Bioengineering 2019Quote: ... The luciferase assay was conducted according to a standard protocol of Ready-To-Grow Dual Secreted Reporter Assay (Clontech Laboratories, Inc., US) except that the amount of substrate was reduced to half of the defined amount.
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bioRxiv - Cancer Biology 2019Quote: ... The assay was performed following manufacturer’s protocol (Takara, Japan) and relative cytotoxicity was calculated according to the same protocol.
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bioRxiv - Immunology 2020Quote: ... The protein concentration was determined by Bradford assay (Takara), and the cell lysate was mixed with 4x Laemmli loading buffer containing β mercapto-ethanol ...
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bioRxiv - Genomics 2024Quote: ... and titer quantified using p24 ELISA antigen assay (Takara). MOI=5 was used to transduce 1x1097 EndoC-βH3 cells in culture media without pen/strep and puromycin.
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bioRxiv - Cell Biology 2019Quote: ... to generate an expression clone Lenti-ALPL. The cDNA encoding β-catenin (GenBank Accession No. NM_007614.3) was amplified by TaKaRa LA TaqR with GC Buffer (TaKaRa ...
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bioRxiv - Genetics 2022Quote: ... color change was measure at OD578 and β-galactosidase units were calculated as described (Yeast Protocol Handbook, Takara Bio).
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bioRxiv - Plant Biology 2022Quote: ... β-galactosidase activity in liquid cultures was assayed using CPRG as substrate as described in the Yeast Protocols Handbook (Takara).
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bioRxiv - Plant Biology 2019Quote: ... Y2H assays were performed using the Matchmaker 3 system (Clontech). The resulting transformants with appropriate positive and negative controls were spotted on SD (-Leu/-Trp ...
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bioRxiv - Cancer Biology 2020Quote: ... WST-1 assays were performed as per manufacturer’s instructions (Takara) (18).
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bioRxiv - Plant Biology 2022Quote: Y2H assays were performed according to the manufacturer’s protocol (Clontech). For Y2H screening ...
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bioRxiv - Plant Biology 2023Quote: Yeast two-hybrid assays were performed using strain AH109 (Takara). Vectors pGADT7 and pGBKT7 were sequentially transformed into this strain ...
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bioRxiv - Synthetic Biology 2021Quote: ... Pseudotyped viruses were quantified with a p24 assay (Takara Bio #632200) per manufacturer’s instructions.
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bioRxiv - Microbiology 2021Quote: ... Y2H assay was performed according to the Yeast Protocols Handbook (Clontech) using the Y2H Gold yeast reporter strain (Clontech) ...
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bioRxiv - Plant Biology 2023Quote: Y1H assay was conducted according to protocol (Clontech, cat. No. 630491). Briefly ...
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bioRxiv - Plant Biology 2023Quote: Y2H assay was performed according to protocol (Clontech, cat. No. 630489). Since BcICE1 self-activates ...
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bioRxiv - Plant Biology 2023Quote: Y2H assays were performed using the MatchmakerTM Two-Hybrid System (Clontech). Bait (pGBKT7 ...
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bioRxiv - Microbiology 2023Quote: ... SEAP activity was quantified using the Great EscAPe SEAP assay (Clontech). Fluorescence was read on a Filtermax F5 plate reader (Molecular Devices).
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bioRxiv - Plant Biology 2024Quote: ... Yeast two-hybrid assays were performed using yeast strain AH109 (Clontech) following standard procedures (Yeast Protocols Handbook PT3024-1 ...
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bioRxiv - Plant Biology 2021Quote: Cell viability assays were performed by using either Y2HGold yeast strains (Clontech) containing a pBridge bait vector or diploid yeast strains generated by mating a Y2HGold strain containing a bait vector with a Y187 strain (Clontech ...
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bioRxiv - Plant Biology 2021Quote: Y2H assays were performed using the Matchmaker™ Two-Hybrid System (Clontech). Bait (pGBKT7 ...
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bioRxiv - Plant Biology 2021Quote: Y2H assays were performed using the Matchmaker GAL4 two-hybrid system (Clontech). The full-length and/or partial (RING ...
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bioRxiv - Immunology 2020Quote: ... The PCR assays were run using LA Taq polymerase (Takara Bio, TAKRR002) with the following thermocycling conditions ...
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bioRxiv - Cancer Biology 2023Quote: ... 10 µL of premix WST-1 cell proliferation assay (Takara Bio, Inc.) was added to each well and the plate was incubated for an additional 2 hours ...
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bioRxiv - Plant Biology 2023Quote: ... Quantitative α-galactosidase assay was also performed according to manufactural instruction (Clontech). Briefly ...
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bioRxiv - Molecular Biology 2024Quote: ... The tetrazolium salts from Premix WST-1 Cell Proliferation Assay System (TAKARA) are cleaved by mitochondrial dehydrogenase in viable cells to produce formazan dye ...
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Flexible linkers in CaMKII control the balance between activating and inhibitory autophosphorylationbioRxiv - Biophysics 2019Quote: Human CaMKII-α (Uniprot_ID: Q9UQM7) and human CaMKII-β (Uniprot_ID: Q13554) were cloned into the pEGFP-C1 vector backbone (Clontech, Mountain View, CA), after modifying the vector to contain a biotinylation sequence (Avitag ...
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bioRxiv - Cell Biology 2023Quote: ... secreted embryonic alkaline phosphatase (SEAP) activity and TGF-β concentration were determined using the Great EscAPe® SEAP (631738, TaKaRa Bio, Kusatsu, Japan) chemiluminescence kit to quantify SEAP activity (excitation wavelength 360 nm ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... Each cDNA sample was amplified for the gene of interest and β-actin in a 15 µL reaction volume TB GreenTM Premix Ex Taq™ II (Takara, Tokyo, Japan). The PCR conditions were 95 °C for 30 s followed by 40 cycles of 95 °C for 5 s and 60 °C for 60 s ...
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bioRxiv - Cell Biology 2020Quote: ... The Y2H assays were performed in the Y2H Gold strain (PT4084-1, Takara 630489 ...
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bioRxiv - Plant Biology 2020Quote: Y1H assays were performed using a Matchmaker™ Gold Y1H System (Clontech, USA) according to the manufacturer’s instructions ...
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bioRxiv - Microbiology 2020Quote: Yeast two-hybrid assay was performed using the Matchmaker Two-Hybrid System (Clontech) by following the manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2020Quote: ... Cellular proliferation was measured with a WST–1 cell proliferation assay system (Takara). WST–1 reagent was added to each well and incubated for 2 hours at 37 °C ...
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bioRxiv - Genetics 2020Quote: Y2H assay was performed using the Matchmaker Gold Yeast Two-Hybrid System (Clontech) according to the manufacturers’ instructions ...
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bioRxiv - Molecular Biology 2024Quote: ... VLP were quantified by a LFA assay for HIV p24 (GoStix Plus, Takara). In this case the number of virions per volume is calculated by the approximate stoichiometry of p24 in VLP (∼2000/virion) ...
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bioRxiv - Cell Biology 2024Quote: Y2H assays were carried out using the Matchmaker two-hybrid system (K1605, Clontech). One of the baits pGBT9 ...
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bioRxiv - Bioengineering 2024Quote: ... VLP were quantified by a LFA assay for HIV p24 (GoStix Plus, Takara). In this case the number of virions per volume is calculated by the approximate stoichiometry of p24 in VLP (∼2000/virion) ...
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bioRxiv - Plant Biology 2020Quote: Yeast two hybrid assay was performed using Matchmaker Gold Yeast Two-Hybrid System (Clontech). The OsSalT gene was cloned in-frame into the EcoRI and BamHI RE sites of the yeast vector ...
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bioRxiv - Plant Biology 2021Quote: ChIP-Seq assays were performed on whole seedlings using anti-GFP antibody (Clontech 632592). Seedlings at 14 DAS (5 g ...
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bioRxiv - Microbiology 2022Quote: ... The RT-qPCR assay was performed using the TB Green Fast qPCR Mix (Takara). The ribosomal protein L21 gene (RPL21 ...
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bioRxiv - Cell Biology 2019Quote: Total protein was extracted from cells by lysing with radioimmunoprecipitation assay buffer (Takara, Japan). Ten micrograms of protein were run on 4–20% gradient polyacrylamide gels (Thermo Fisher ...
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bioRxiv - Plant Biology 2022Quote: Yeast one-hybrid assays were performed using the Matchmaker Yeast One-Hybrid System (Clontech). The DNA fragment 1,000 bp upstream of the start codon ATG of DDRM2 was cloned into pHis2.1 vector and SOG1 was cloned into the pGADT7 vector ...
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bioRxiv - Microbiology 2023Quote: RNA obtained from the RIP assay sample was purified using NucleoSpin RNA Plus (TaKaRa), followed by library construction and RNA-seq analysis ...
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bioRxiv - Plant Biology 2023Quote: Y2H assays were performed with the MatchMaker GAL4 Two-Hybrid System 3 (Takara Bio). Saccharomyces cerevisiae strain AH109 was co-transformed with different pairs of pGADT7 and pGBKT7 harboring IREH1 or B1-Rafs ...