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Citations for Takara Bio :
1 - 50 of 6565 citations for Mouse Direct PCR Kit For Genotyping since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2022Quote: ... Genotyping was performed using Terra PCR Direct Genotyping Kit (Takara, 639285) following protocol modified from The Jackson Laboratory ...
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bioRxiv - Cell Biology 2024Quote: ... genomic DNA was purified with Terra™ PCR Direct Genotyping Kit (Takara), followed by PCR amplification and Sanger sequencing of specific genomic regions encompassing the target site.
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bioRxiv - Microbiology 2022Quote: ... Genotyping was performed using Terra PCR Direct Polymerase Mix (Takara) following manufacturers specifications.
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bioRxiv - Microbiology 2024Quote: ... Genotyping was performed using Terra PCR Direct Polymerase Mix (Takara) following the manufacturer’s specifications ...
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bioRxiv - Developmental Biology 2023Quote: ... Genotyping was done using the Terra™ PCR Direct kit (Takara Bio, Kusatsu, Shiga Japan). Embryonic sex and Lhx2 allele genotyping were performed as mentioned [26] ...
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bioRxiv - Cancer Biology 2022Quote: Genotyping was done using the Terra PCR Direct Polymerase Mix and kits (Takara Bio; Shiga, Japan). The primers used for genotyping are given in supplementary table 1 ...
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bioRxiv - Plant Biology 2023Quote: ... Primers flanking the region targeted for replacement were designed and used for Terra Direct genotyping PCR (Clontech) to identify transformants lacking the wild-type fragment ...
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bioRxiv - Microbiology 2019Quote: PCR were performed using the Terra PCR Direct Polymerase Mix (Clontech) directly on the cell lysates using the following primers:
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bioRxiv - Microbiology 2019Quote: PCR were performed using the Terra PCR Direct Polymerase Mix (Clontech) directly on the cell lysates after each round of co-infection ...
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bioRxiv - Genomics 2020Quote: ... cDNA was PCR-amplified using Terra PCR Direct Polymerase (Takara Bio). Final libraries were prepared using 1ng of cDNA per library with the Nextera XT kit (Illumina ...
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bioRxiv - Cancer Biology 2024Quote: ... Barcode sequence of each clone was PCR amplified directly from the cells using Terra PCR Direct Polymerase kit (Takara) for Sanger sequencing using previously described primers (5).
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bioRxiv - Neuroscience 2020Quote: ... each cDNA was subjected to PCR genotyping using Emerald AMP HS PCR Master Mix (Takara Bio USA #RR330B) and primers shown in Supplementary Table S5 ...
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bioRxiv - Microbiology 2023Quote: ... a PCR test is performed using the Terra PCR Direct Polymerase Mix (Takara Bio Europe SAS ...
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bioRxiv - Developmental Biology 2022Quote: ... PCR was done using the Terra™ PCR Direct PCR mix (Takara Bio, Kusatsu, Shiga Japan). Genotyping for Lhx2 was performed as described earlier (Mangale et al. ...
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bioRxiv - Microbiology 2023Quote: ... The cells were cultured again overnight and subjected to the quantification of mRNA by qRT-PCR using the CellAmp Direct RNA Prep Kit for RT-PCR (Real Time) (3732, Takara Bio Inc.), One Step TB Green PrimeScript PLUS RT-PCR Kit (Perfect Real Time ...
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Cholesterol deprivation induces TGFβ signaling to promote basal differentiation in pancreatic cancerbioRxiv - Cancer Biology 2019Quote: ... The PCR reaction was performed using Terra PCR Direct Red Dye Premix (#639286, TaKaRa) for LSL-KRas construct detection and GoTaq Green Master Mix (#M7122 ...
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bioRxiv - Genomics 2021Quote: Genotyping PCR were performed using 100 ng of gDNA and LA Taq DNA polymerase (Takara), with the following cycling conditions ...
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bioRxiv - Microbiology 2023Quote: ... total RNA was collected using a CellAmp Direct RNA Prep Kit for RT-PCR (Real Time) (TaKaRa, Cat# 3732) according to the manufacturer’s instructions ...
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bioRxiv - Microbiology 2023Quote: ... total RNA was collected using a CellAmp Direct RNA Prep Kit for RT-PCR (Real Time) (TaKaRa, Cat# 3732). GAPDH and vRNA levels were measured with a RT-qPCR assay using the One Step TB Green PrimeScript PLUS RT-PCR Kit (Perfect Real Time) ...
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bioRxiv - Genomics 2020Quote: ... Used cDNA template was further split into two PCR reactions with Terra PCR Direct Polymerase (Takara) with the following program 98°C for 2min ...
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bioRxiv - Microbiology 2023Quote: ... Genotyping PCR reactions were performed on 10ng of isolated parasite DNA using CloneAmp HiFi polymerase premix (Takara) in an Applied Biosystems thermocycler according to manufacturer’s instructions.
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bioRxiv - Genetics 2019Quote: ... after a first step of Terra PCR Direct Polymerase Mix amplification (Takara) using one μl sample of total blood ...
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bioRxiv - Genomics 2023Quote: ... a preliminary Terra PCR Direct Polymerase mix amplification (Takara Bio, Kusatsu, Japan) using 1µL of crude sample was used for direct genotyping without DNA purification ...
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bioRxiv - Microbiology 2022Quote: Total RNA was extracted from HT-29 cells using the CellAmp Direct RNA Prep Kit for RT-PCR (3732; TaKaRa, Japan) after the introduction of genomic DNA or Poly (I ...
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bioRxiv - Evolutionary Biology 2023Quote: ... The genotyping was performed using PCR and 1.5% agarose gel electrophoresis using EX Taq Hot Start Version (TaKaRa Bio) according to the manufacturer’s protocol and two designed primers ...
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bioRxiv - Physiology 2023Quote: ... DNA was amplified using Terra PCR Direct Red Dye Premix (Takara Cat. No. 639286) and ran on a 1% agarose gel to confirm DNA recombination ...
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bioRxiv - Developmental Biology 2019Quote: ... followed by genotyping using Taq DNA polymerase (Takara) according to manufacturer’s instructions ...
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bioRxiv - Cell Biology 2021Quote: ... cDNA was further amplified by adding 40 μl of a PCR mix containing 0.03 U/μl of Terra PCR direct polymerase (Takara Bio), 1.25x Terra PCR Direct Buffer ...
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bioRxiv - Molecular Biology 2020Quote: ... The sgRNA target locus on exon 3 or 5 was PCR amplified with Terra™ PCR Direct Polymerase Mix (Takara) for 35 cycles using primer sets mEX3F and mEX3R or mEX5F and mEX5R (Table 1) ...
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bioRxiv - Neuroscience 2022Quote: ... washed with 200 µL water, and resuspended in PCR mix (22 µL water, 25 µL Terra PCR direct buffer, 1 µL Terra Polymerase (Takara), 1 µL 100 M Truseq PCR handle primer ...
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bioRxiv - Cell Biology 2022Quote: ... cDNA was amplified by PCR by adding 1 µl of the Terra PCR Direct Polymerase (1.25 U/µl, Takara 639270), 25 µl of the Terra PCR Direct buffer and 1 µl of the ISPCR primer (10 µM stock concentration ...
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bioRxiv - Immunology 2023Quote: ... which was measured using a real-time PCR assay with a SARS-CoV-2 direct detection RT‒qPCR kit (Takara Bio, Siga, Japan). The IC50 was calculated by IC50 Calculator (https://www.aatbio.com/tools/ic50-calculator ...
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bioRxiv - Immunology 2020Quote: Mouse tail DNA was amplified using KAPA Taq PCR Kit (Takara Bio, cat. R040A) and primers Rosa26 WT F ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... intracellular RNA was extracted with CellAmp direct RNA prep kit (TAKARA) according to the manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2022Quote: ... A 0.5 µl aliquot of the resulting genomic DNA solution was used as the template for genotyping purposes in 25 µl PCR reactions with Ex Taq DNA polymerase (TaKaRa, RR001). PCR products were subcloned with the TOPO TA cloning Kit (Invitrogen ...
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bioRxiv - Microbiology 2021Quote: ... and 0.7 U of Terra PCR Direct Polymerase (ClonTech Europe, Saint-Germain-en-Laye, France).
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bioRxiv - Synthetic Biology 2023Quote: ... PrimeSTAR Max DNA Polymerase and Phire Plant Direct PCR Master Mix were purchased from Takara Bio and Thermo Fisher Scientific respectively ...
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bioRxiv - Developmental Biology 2023Quote: ... At 48 hpf the GFP + progeny was genotyped using a forward primer in the foxo1a endogenous locus and a reverse primer in the EGFP inserted transgene using a touch-down PCR with the Terra™ PCR Direct Polymerase Mix (639270; Takara). The PCR product was run in a 1% agarose gel to validate its size and the remaining product was sent for Sanger sequence.
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bioRxiv - Cell Biology 2024Quote: ... The absence of ATG14 transcripts was verified by Reverse Transcription PCR (RT-PCR) on cDNA synthesized from total RNA extracted from the cell samples using Terra™ PCR Direct Polymerase Mix (TAKARA, 639270). Total RNA was isolated using the NucleoSpin RNA II kit (Macherey-Nagel) ...
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bioRxiv - Immunology 2021Quote: ... which involves the following steps: i) cDNA amplification (Terra PCR direct Polymerase, Takara, cat. no. 639270), ii ...
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bioRxiv - Developmental Biology 2023Quote: ... cDNA was amplified with V3d(T)24 and R2SP primers and Terra PCR Direct Polymerase (Clontech) for 16 cycles ...
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bioRxiv - Microbiology 2021Quote: ... recombined by PCR (TaKaRa PCR kit (Clontech Laboratories)) using the primers and cycle conditions in Table S1C ...
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bioRxiv - Microbiology 2021Quote: ... recombined by PCR (TaKaRa PCR kit (Clontech Laboratories)) using the primers and cycle conditions in Table S1C ...
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bioRxiv - Plant Biology 2020Quote: ... Genotyping was carried out using TAKARA EX taq (Takara Bio, USA) according to the manufacturer’s instructions.
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bioRxiv - Cancer Biology 2023Quote: ... sublines 14D7 and 24C7.8 The presence of the deletion on the translocated and the wildtype allele was validated by PCR using the Terra PCR Direct Polymerase Mix (Takara Bio Europe; supplemental Table 7). From line 14D7 ...
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bioRxiv - Cell Biology 2021Quote: ... SYBR Green PCR kit (Takara) was applied to conduct RT-qPCR ...
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bioRxiv - Microbiology 2020Quote: Total RNA was isolated from A549 cells using a CellAmp Direct RNA Prep Kit (TaKaRa), as well as murine pharyngeal and lung tissues using an RNeasy Fibrous Tissue Mini Kit (QIAGEN) ...
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bioRxiv - Developmental Biology 2021Quote: ... DNA from these samples was screened by genotyping with Z-Taq (Takara R006B). Embryos identified as homozygous for wild type or HoxDinν2 alleles were processed individually for ATAC-Seq ...
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bioRxiv - Biochemistry 2019Quote: ... The PCR products were extracted with PCR purification kits (Takara 9761) and ligated using a Blunting Kination Ligation Kit (Takara 6217) ...
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bioRxiv - Cell Biology 2021Quote: ... and PCR Clean-Up Kit (Takara) then In-Fusion (Takara ...