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Citations for Takara Bio :
101 - 150 of 1168 citations for Mouse CDKL3 shRNA Plasmid since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2021Quote: ... MYC (mouse, Clontech, 631206 ...
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bioRxiv - Bioengineering 2021Quote: ... coli BL21 (DE3) Gold cells harboring the corresponding expression plasmid and the TaKaRa chaperone-plasmid pTF16 (TAKARA BIO INC.) were used to inoculate 500 mL of autoinduction medium (464 mL LB medium ...
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bioRxiv - Cell Biology 2020Quote: ... the sgRNA plasmid and a plasmid expressing the Cas9 nuclease were transfected using the Xfect Transfection Reagent (631318, Takara). A Cas9 expression plasmid without a puromycin resistance was generated by removing puromycin from pAc-sgRNA-Cas9 (addgene# 49330) ...
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bioRxiv - Cancer Biology 2022Quote: ... the recombinant pAAV-CRE plasmid was packaged in HEK293T cells using pHelper and pRC2-mi342 plasmids (Takara, Catalog #632608). Cells were harvested three days after transfection and AAV2 was isolated using AAVpro Extraction Solution (Takara ...
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bioRxiv - Neuroscience 2023Quote: ... mCherry was introduced into the pcDNA™3.1-BspEI-LRP10 plasmid via PCR amplification from the pmCherryN1 plasmid (Clontech) via the addition of BspEI restriction sites in the primer sequences ...
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bioRxiv - Genomics 2023Quote: ... The recombinant pAAV-CRE plasmid was packaged in HEK293T cells using pHelper and pRC2-mi342 plasmids (Takara, Catalog #632608). Cells were harvested three days after transfection and AAV2 was isolated using AAVpro Extraction Solution (Takara ...
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bioRxiv - Evolutionary Biology 2021Quote: ... then removed from the pGEM shuttle plasmid using EcoRI and NotI enzymes and annealed into the DFRS empty plasmid pre-amplified (DFRS empty plasmid primers) and digested by the same enzymes (In-Fusion Kit; Clontech). The control cassette was thus placed on the 3’UTR of the RFP gene.
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bioRxiv - Microbiology 2021Quote: ... The retroviral reporter plasmids encoding a Gaussia luciferase reporter gene were constructed by cloning the reporter genes into the pQCXIP plasmid (Clontech). DNA fragments encoding C-terminally S-tagged ACE2 orthologs were synthesized in pUC57 backbone plasmid by Sangon Biotech (Shanghai ...
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bioRxiv - Cell Biology 2021Quote: ... GCN4-scFv-sfGFP were amplified from addgene plasmid #60906 and introduced into plasmid#132397 through in-fusion cloning (Takara Bio). All other plasmids in this study are derived from a plasmid that contains a CAG promoter for constitutive expression ...
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bioRxiv - Microbiology 2022Quote: ... Each of these prey plasmids and previously constructed bait plasmids were co-transformed into Y2HGold yeast strain using Yeastmaker Yeast Transformation System 2 (TaKaRa). The transformation mix was spread on DDO/X and QDO/X/A agar plates and incubated at 30°C for 3-5 days ...
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bioRxiv - Cell Biology 2020Quote: ... was co-transfected with packaging plasmid (pCAG-HIVgp) and VSV-G/Rev-expressing plasmid (pCMV-VSVG-RSV-Rev) into HEK293T cells (Clontech). Cells expressing Involucrin-mCherry were selected by 2 µg/ml Puromycin treatment.
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bioRxiv - Genetics 2019Quote: ... To generate the plasmid pEGFP-N2-pB-min we removed the multiple cloning site (MCS) of the pEGFP-N2 plasmid (Clontech) by BamHI-NheI double digestion ...
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bioRxiv - Microbiology 2023Quote: ... The retroviral reporter plasmids encoding a Gaussia luciferase reporter gene were constructed by cloning the reporter genes into the pQCXIP plasmid (Clontech). Plasmids encoding soluble ACE2 variants fused with human IgG1 Fc were described in our previous study20 ...
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bioRxiv - Cancer Biology 2023Quote: ... The generated lentiviral plasmids were cotransfected with viral packaging plasmids PAX2 and pMD2.G into HEK 293T Lenti-X cells (Clontech) using TransIT-LT1 (Mirus Bio LLC ...
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bioRxiv - Cancer Biology 2024Quote: ... approximately 20% confluent HEK293T cells seeded in 12-wells plates were transfected with 300ng ZNRF3 encoding plasmid and 50ng GFP expression plasmid (pEGFP-C1, Clontech) using Lipofectamine 2000 ...
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bioRxiv - Physiology 2024Quote: The pGG2-TNNT2-HA-TTL and the pAAV-TNNT2-Empty plasmids were generated from the pGG2-TNNT2-Mut1 plasmid (VC206) by InFusion Cloning (Takara Clontech). In brief ...
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bioRxiv - Plant Biology 2019Quote: ... were transferred into the pCold ProS2 plasmid (TaKaRa) harboring a gateway reading flame cassette (Invitrogen) ...
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bioRxiv - Molecular Biology 2020Quote: ... pECFP and pEYFP plasmids were purchased from Clontech Laboratories (Palo Alto ...
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bioRxiv - Biochemistry 2019Quote: ... This plasmid was used along with pGro7 (Takara) to transform Shuffle T7 Express cells (NEB) ...
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bioRxiv - Bioengineering 2021Quote: ... The original pAUR101 plasmid was purchased from Takara Bio ...
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bioRxiv - Biochemistry 2020Quote: pEGFP-N1 plasmid (Clontech, Mountain View, California, US) was transformed into XL1-Blue [dut+ ...
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bioRxiv - Biochemistry 2021Quote: ... plasmid backbones derived from EYFP-N1 (Clontech/Takara), Lenti-Cas9-blast (a kind gift of F ...
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bioRxiv - Biochemistry 2021Quote: ... plasmid backbones derived from EYFP-N1 (Clontech/Takara), Lenti-Cas9-blast (a kind gift of F ...
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bioRxiv - Neuroscience 2022Quote: ... were transformed with the plasmid peGFP-C1 (Clontech). Bacteria were grown overnight in lysogeny broth (LB ...
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bioRxiv - Cell Biology 2022Quote: Plasmid DNA (pEGFP-N1, 5kb (Clontech, 6085-1) encoding a cytomegalovirus (CMV ...
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bioRxiv - Cell Biology 2020Quote: ... packaging plasmid using CalPhos Mammalian Transfection Kit (Takara). Next day ...
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bioRxiv - Bioengineering 2021Quote: ... The original pAUR101 plasmid was purchased from Takara Bio ...
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bioRxiv - Molecular Biology 2019Quote: ... Two-hybrid plasmids were based on pGBT9 (Clontech) or the pGAD-C- and pGBD-C-series ...
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bioRxiv - Developmental Biology 2019Quote: ... The plasmids were then digested with DNaseI (Takara) for 30 min at 37°C ...
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bioRxiv - Developmental Biology 2020Quote: ... from pEGFP-C1 and pDsRed2-N1 plasmid (Clontech). The fragment bounded by the AseI and NheI restriction sites was deleted from pEGFP-C1 and pDsRed2-N1 and the upstream sequences of Atoh7 (2220 bp in length ...
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bioRxiv - Biochemistry 2020Quote: ... Five micrograms of reporter plasmid pEGFP-C2 (Clontech), coding the green fluorescent protein ...
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bioRxiv - Neuroscience 2019Quote: ... Additional plasmids were created using InFusion cloning (Takara). The InFusion cloning module (SnapGene ...
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bioRxiv - Neuroscience 2020Quote: ... the CMV-EGFP plasmid (pEGFP; Clontech, CA, USA) was used as a positive control ...
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bioRxiv - Immunology 2021Quote: ... A plasmid encoding tdTomato was obtained from Takara Bio (#632533 ...
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bioRxiv - Cell Biology 2021Quote: Plasmids used for transfections include pmCherry-C1 (Clontech), pLAMP1-mGFP (Addgene #34831 ...
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bioRxiv - Cell Biology 2023Quote: ... plasmid backbones derived from EYFP-N1 (Clontech/Takara), Lenti-Cas9-blast (a kind gift of F ...
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bioRxiv - Cell Biology 2023Quote: ... plasmid backbones derived from EYFP-N1 (Clontech/Takara), Lenti-Cas9-blast (a kind gift of F ...
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bioRxiv - Biochemistry 2023Quote: The reporter plasmids were linearized with XbaI (Takara). Subsequently ...
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bioRxiv - Neuroscience 2023Quote: ... mCherry-C1 plasmid (Clontech, Mountain View, CA, USA) at 1 ng/μl ...
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bioRxiv - Immunology 2023Quote: ... plasmids were transformed into Stellar cells (Takara Bio), isolated by Maxiprep kits (Macherey Nagel) ...
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bioRxiv - Physiology 2024Quote: The plasmids were linearized with NotI (Takara Bio), and capped RNAs (cRNAs ...
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bioRxiv - Cell Biology 2023Quote: ... pEGFP-C1 plasmid construct was purchased from Clontech. Flag-CSA construct subcloned in pcDNA3.1 vector was a kind gift Dr ...
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bioRxiv - Neuroscience 2022Quote: ... Plasmid expressing MeCP2 and GFP tagging was made by cloning the cDNA into pLVX-EF1α-IRES-ZsGreen1 vector (TaKaRa plasmid #631982). R168X mutation was introduced by site-directed mutagenesis ...
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bioRxiv - Microbiology 2020Quote: ... Construction of strains with cytoplasmic sGFP expression was performed by cloning the sGFP expression cassette from plasmid pIGPAPA and the neo cassette from plasmid pSD1 to the polylinker of plasmid pBluescript II (using the T4 DNA ligase, Takara Bio). The resulting plasmid pBS-GFP-gen was used to transform V ...
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bioRxiv - Microbiology 2022Quote: ... and the plasmid DNAs were isolated using the NucleoBond® Xtra Midi EF plasmid mid-prep kit (Takara Bio USA Inc.) according to the manufacturer’s instructions.
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bioRxiv - Synthetic Biology 2022Quote: Single point mutations in all mOptoT7 plasmids were based on previously published plasmids (mOptoT7 Version 1, V1)53 and created using CloneAmp HiFi PCR Premix (Takara Bio). All constructs were transformed into Top10 E ...
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bioRxiv - Cell Biology 2023Quote: ... and constructs were isolated from 50 ml bacterial cultures using a plasmid DNA extraction kit (Takara Bio USA, Inc. NucleoSpin Plasmid). DNA concentrations were measured by a NanoDrop spectrophotometer ...
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bioRxiv - Physiology 2024Quote: The pGG2-TNNT2-HA-TTL and the pAAV-TNNT2-Empty plasmids were generated from the pGG2-TNNT2-Mut1 plasmid (VC206) by InFusion Cloning (Takara Clontech). In brief ...
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bioRxiv - Developmental Biology 2020Quote: ... mouse anti dsRed (Clontech) 1:200 ...
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bioRxiv - Neuroscience 2022Quote: ... mouse anti-mCherry (Takara Bio ...