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Citations for Takara Bio :
401 - 450 of 1168 citations for Mouse CDKL3 shRNA Plasmid since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cancer Biology 2023Quote: ... and mouse anti-SPARC/Osteonectin (ON1-1, Takara) for 1 h at RT ...
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bioRxiv - Biochemistry 2022Quote: ... plasmids encoding trcP mutants were generated with the PrimeSTAR Mutagenesis Basal Kit (TAKARA-bio).
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bioRxiv - Biochemistry 2022Quote: ... as used before.11 TSC were cotransfected with GFP-expressing plasmid peGFP-CI (Clontech) and sorted for GFP-positive cells in a 96 well plate at the central research facility cell sorting at MHH ...
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bioRxiv - Neuroscience 2021Quote: ... transfected at 5-7 DIV with the plasmid peGFP-N1 (Clontech, Mountain View, CA) using lipofectamine 2000 (ThermoFisher Scientific) ...
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bioRxiv - Cell Biology 2019Quote: ... The following plasmids were used for transfection: pEGFP-N1 (Clontech Cat. No. 6085-1), pCDNA3.1 N-Myc-TNAP and pCDNA3-mCherry TNAP ...
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bioRxiv - Cell Biology 2019Quote: ... All plasmids were assembled with the In-Fusion enzyme according to manufacturer’s protocol (Clontech). RNAi constructs were made by using the Gateway system (Invitrogen ...
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bioRxiv - Microbiology 2019Quote: ... The resultant plasmid was cloned into Escherichia coli JM109 competent cells (TAKARA, Shiga, Japan) and transformed into S ...
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bioRxiv - Microbiology 2019Quote: ... The constructed plasmid was cloned into Escherichia coli JM109 competent cells (TAKARA, Shiga, Japan) and pre-introduced into restriction-deficient S ...
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bioRxiv - Microbiology 2019Quote: Preparative DNA for plasmid cloning and PCR fusion constructs was amplified with CloneAmp (Takara) using the following cycle conditions ...
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bioRxiv - Biophysics 2019Quote: ... 300 ng of the plasmid encoding the green fluorescent protein (pEGFP, Clontech, CA, USA) and 12 μL of Lipofectamine/Plus reagent ...
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bioRxiv - Cancer Biology 2019Quote: ... DNA plasmids were collected from the competent bacteria using MidiPrep (Clontech, Mountain View, CA).
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bioRxiv - Cancer Biology 2021Quote: ... Plasmids were maxipreped by using NucleoBond Xtra EF kits (Takara Bio USA, 740424-50) according to manufacturer’s protocol ...
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bioRxiv - Cell Biology 2021Quote: ... The human fimbrin sequence was cloned into a pEGFP-C1 plasmid (Clontech; 6084-1). The human ezrin sequence was cloned into a pEGFP-N1 (Clontech ...
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bioRxiv - Cell Biology 2020Quote: ... 293FT cells were transfected with the pLV constructs along with packaging plasmids pVSVG (Clontech) and pLV-CMV-delta 8.2 (Massey ...
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bioRxiv - Neuroscience 2022Quote: ... they were placed under the binocular and 250 nl of pEGFP-C1 plasmid (Clontech) were injected through the median ocellus following the course of the ocellar tract ...
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bioRxiv - Cancer Biology 2020Quote: ... The amplified PCR product was inserted into EcoRI-linearized pLVX TetOne-puro plasmid (Clontech) using the NEBuilder HiFi Assembly kit (New England BioLabs) ...
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bioRxiv - Biochemistry 2020Quote: ... The product was cloned into pET28a plasmid using In-Fusion PCR Cloning Kit (Clontech), with an N-terminal hexahistidine (His6 ...
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bioRxiv - Genetics 2019Quote: ... Plasmids were co-transformed into yeast strain EGY48 according to the manufacturer’s manual (Clontech). Transformed yeast was plated onto a synthetic medium DDO (-Ura/-Trp) ...
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bioRxiv - Bioengineering 2020Quote: ... Each cDNA was cloned to EcoRV site of pAID plasmid by In fusion(Takara) cloning.
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bioRxiv - Developmental Biology 2020Quote: ... The CMV-MitoDsRed2 (pDsRed2-Mito) and CMV-MitoGFP (pAcGFP1-Mito) plasmids are from Clontech. Atoh7-MitoDsRed2 was designed using UGene software (Unipro) ...
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bioRxiv - Physiology 2021Quote: Plasmid DNA encoding enhanced green fluorescent (EGFP)-actin (Takara Bio USA, Mountain View, CA) facilitated exogenous gene expression in rodent kidneys ...
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bioRxiv - Neuroscience 2019Quote: Neurons at 22 DIV were transfected with an eGFP-N2 plasmid (Clontech, Kyoto, Japan) using Lipofectamine 2000 for 24 hours ...
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bioRxiv - Plant Biology 2020Quote: ... and the plasmid was re-circularised using In-Fusion HD enzyme (Clontech Laboratories Inc).
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bioRxiv - Microbiology 2020Quote: ... The mammalian expression plasmid pHEK293 Ultra Expression Vector II (Takara Bio Inc., Shiga, Japan) was also inserted with the N or GPC ORF at the BamHI site of multicloning sites ...
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bioRxiv - Cell Biology 2020Quote: ... VAMP2-tagRFP was generated by cloning from VAMP2-pHluorin into the tagRFP plasmid (Clontech). VAMP21-96-tagRFP was created by PCR of VAMP2 (AA 1-96 ...
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bioRxiv - Microbiology 2020Quote: ... The plasmid used for disruption was constructed using the In-Fusion cloning kit (Clontech Laboratories ...
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bioRxiv - Molecular Biology 2020Quote: ... The plasmids were co-transformed pairwise into AH109 yeast strains (Matchmaker 3 System, Clontech), and selected initially on double drop-out (DDO ...
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bioRxiv - Neuroscience 2022Quote: ... triple transfection of HEK-293T cells was performed using the pHelper plasmid (TaKaRa, USA), trans-plasmid with PHP.B capsid (Ben Deverman ...
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bioRxiv - Cancer Biology 2022Quote: ... 2 µg of plasmids were diluted in 200 µL Xfect transfection reagent (631317, Takara). The mixture was incubated at room temperature for 15 min ...
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bioRxiv - Genetics 2022Quote: ... Cas9 was amplified from the expression plasmid using CloneAmp HiFi PCR Premix (Takara Bio) for 35 cycles (98□ for 10 s ...
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bioRxiv - Molecular Biology 2023Quote: ... were subcloned in frame from their corresponding SMAUG1-HisTEV2xFLAG plasmids into pEGFP-N1 (Clontech) using XhoI and BamHI ...
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bioRxiv - Synthetic Biology 2023Quote: ... here we inserted the two chaperones GroES and GroEL from the pGro7 plasmid (TaKaRa). The sequence for GroES and GroEL were amplified from the pGro7 plasmid by PCR and inserted into the linearized plasmid with NpnJ by In-Fusion® ...
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bioRxiv - Synthetic Biology 2023Quote: Plasmids used in this study were constructed using In-Fusion® Cloning Technology (TaKaRa). Primers and genes were ordered from Integrated DNA Technologies (IDT) ...
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bioRxiv - Genomics 2023Quote: ... Lentivirus containing the overexpression plasmid was generated using LentiX Packaging Single Shots (Clontech, 631278) according to the manufacturer’s protocol ...
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bioRxiv - Neuroscience 2023Quote: ... GFP-tagged human tauKQ and tauP301L were expressed from the pEGFP-C1 plasmid (Clontech). Expression of mApple or GFP in cultured neurons was done using pGW1 mApple and pEGFP-C1 plasmids ...
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bioRxiv - Microbiology 2023Quote: ... These recombinant plasmids were transformed into Escherichia coli BL21 (Takara Bio Co Ltd, Japan) and the transformed cells were cultured at 37°C until the OD600 reached at 0.6–0.8 ...
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bioRxiv - Bioengineering 2023Quote: ... and AAV6 were prepared using the AAVpro® Packaging Plasmid (Takara Bio, Shiga, Japan), while AAV4 ...
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bioRxiv - Genomics 2023Quote: ... cells were co-transfected with 1 µg of pEGFP-N1 plasmid (Takara Bio USA). Where indicated ...
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bioRxiv - Biochemistry 2023Quote: The plasmid library was transformed into Escherichia coli strain BL21(DE3)-pGro7/GroEL (TaKaRa). Cells were grown overnight at 37°C on nitrocellulose membranes (Amersham™ Protran™ ...
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bioRxiv - Plant Biology 2023Quote: ... Purified PCR products were cloned into pYAO::hSpCas9 plasmid via In-Fusion (Takara, 639650).
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bioRxiv - Cell Biology 2023Quote: ... Human CXCR4 was cloned into the pAcGFPm-N1 plasmid (Clontech Laboratories, Palo Alto, CA), as described (20).
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bioRxiv - Cell Biology 2023Quote: ... we used the pRetroX-Tet-on-advanced and pRetrox-Tight-Puro plasmids from Clontech Inc ...
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bioRxiv - Cancer Biology 2023Quote: ... Plasmid extraction was performed using the NucleoBond Xtra Midi EF kit (Takara Bio, 740420.10).
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bioRxiv - Biophysics 2023Quote: ... triple transfection of HEK-293T cells was performed using the pHelper plasmid (TaKaRa, USA), trans-plasmid with either the AAV2/9 ...
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bioRxiv - Immunology 2023Quote: ... As a negative control for pull-downs assays the eGFP plasmid (Clontech Laboratories, USA) was transfected.
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bioRxiv - Cell Biology 2023Quote: All plasmids used in this study were generated using the Infusion cloning system (Takara) with primers containing 5’ flanking regions (15bp ...
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bioRxiv - Bioengineering 2024Quote: cDNA and the plasmid library were amplifed using CloneAmp PCR Master Mix (Takara 639298) and primers for amplicon sequencing (Supplemtary Note 23) ...
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bioRxiv - Cell Biology 2021Quote: ... and the mouse Mon1b cDNA was amplified from the Marathon-Ready adult mouse brain and testis cDNAs (Clontech/Takara Bio, Shiga, Japan) by conventional PCR techniques ...
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bioRxiv - Cell Biology 2021Quote: ... and the mouse Mon1b cDNA was amplified from the Marathon-Ready adult mouse brain and testis cDNAs (Clontech/Takara Bio, Shiga, Japan) by conventional PCR techniques ...
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bioRxiv - Microbiology 2021Quote: ... mouse monoclonal antibody against EGFP (Clontech, Mountain View, CA) was used for western blotting at a dilution of 1:1,000 and rabbit polyclonal antiserum reactive against protein kinase A (PKA ...