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Citations for Takara Bio :
51 - 100 of 964 citations for Marker 7 Pcb Mixture W Pcb 118 13C12 99% 100 Ng Ml In Nonane since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Genomics 2021Quote: Genotyping PCR were performed using 100 ng of gDNA and LA Taq DNA polymerase (Takara), with the following cycling conditions ...
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bioRxiv - Molecular Biology 2019Quote: ... 10 μL PCR mixture (TaKaRa), 1 μL upstream primer (10 μmol/L) ...
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bioRxiv - Neuroscience 2022Quote: ... This Nptx2-Nptxr expression vector and linear Hygromycin marker (Clontech) were cotransfected into CHO-tetON cells (Clontech) ...
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bioRxiv - Genomics 2024Quote: ... together with three DNA markers (λ-Hind III digest, Takara Cat ...
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bioRxiv - Genomics 2024Quote: ... together with three DNA markers (λ-Hind III digest; Takara Cat ...
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bioRxiv - Plant Biology 2020Quote: ... and 200 ng/mL AbA (Takara Bio USA, Inc., Mountain View, US-CA) were added when they are required ...
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bioRxiv - Genomics 2022Quote: ... 100 ng of polyA selected RNA from the human lung carcinoma cell line A549 (Takara #636141) was used as input ...
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bioRxiv - Genomics 2022Quote: ... 100 ng of RNA was reverse transcribed into cDNA using PrimerScriptTM RT Master Mix (TaKaRa, #RR036A). Next ...
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bioRxiv - Microbiology 2019Quote: ... cDNA was synthesized with 100 ng RNA using Advantage RT-PCR Kit (Clontech, Mountain View, USA). Reaction condition for real time PCR was 95°C for 3min (1 cycle) ...
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bioRxiv - Molecular Biology 2023Quote: ... or 100 ng of genomic DNA was amplified with Advantage GC 2 polymerase (Takara Bio 639114) using 1 M GC Melt and primers spanning the region from upstream of the splice donors to the 3’ end of the repeat ...
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bioRxiv - Biochemistry 2022Quote: ... the cleavage mixture was applied to a gravity flow column packed with 8 mL TALON cobalt resin (Takara) equilibrated in AEX buffer ...
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bioRxiv - Molecular Biology 2023Quote: ... Cells were transduced on RetroNectin (100 µg/mL; Takara)-coated plates at an MOI of 5 and the transduced population was enriched by puromycin (3 µg/mL ...
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bioRxiv - Microbiology 2021Quote: ... PCR was performed in 96-well plates and each well containing up to 10 µg of gDNA in a total of 100 µL reaction mixture consisting of Titanium Taq DNA polymerase buffer and enzyme (Takara, #639209), deoxynucleoside triphosphate ...
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A randomized multiplex CRISPRi-Seq approach for the identification of critical combinations of genesbioRxiv - Genetics 2023Quote: ... to OD600 0.2–0.3 with 2–3 mL fresh AYE +Fe +Cys containing 40 ng/mL anhydrous tetracycline (aTC, Clontech 631310). On the third day ...
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bioRxiv - Genomics 2020Quote: ... 10 μL of Mixture (TaKaRa, Japan), 1 μL upstream primers (10 μmol/L ...
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ACE2-lentiviral transduction enables mouse SARS-CoV-2 infection and mapping of receptor interactionsbioRxiv - Microbiology 2021Quote: ... The p24 equivalent (ng/ml) was measured using Lenti-X GoStix Plus (Takara Bio) as per manufacturer instructions.
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bioRxiv - Bioengineering 2022Quote: ... PDGF-aa (20 ng/ml; R&D) is also supplemented to RHB-A (Takara). This medium composition will be referred to as RHB-A complete ...
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bioRxiv - Cell Biology 2019Quote: ... We co-transfected linearized eYFP-PCNA and linearized hygromycin marker (Clontech) with FuGENE6 (Promega ...
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bioRxiv - Biochemistry 2022Quote: ... along with a linear selection marker of hygromycin using Xfect (Clontech). These cells were cultured in a medium containing 200 µg/mL G418 sulfate (Santa Cruz Biotechnology) ...
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bioRxiv - Cancer Biology 2022Quote: ... amplified from the linear puro marker (Cat. No. 631626, Takara-Bio), was inserted using the In-fusion HD cloning kit (Cat ...
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bioRxiv - Molecular Biology 2024Quote: ... and the human herpes simplex virus 5 puromycin resistance marker (Clontech).
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bioRxiv - Microbiology 2023Quote: ... The protein-bead mixtures were then loaded onto a gravity column (TALON 2 mL Disposable Gravity Column, Takara #635606). Lysate was loaded into the column 5 times with gravity flow and then washed in a 1M NaCl buffer 3 times ...
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bioRxiv - Plant Biology 2020Quote: ... cDNA was synthesized from 100 ng of RNA using a PrimeScript 1st strand cDNA Synthesis Kit (Takara). The resulting cDNA was quantified with a LightCycler 480 (Roche ...
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bioRxiv - Molecular Biology 2021Quote: ... PCR was performed on 100 ng genomic DNA using PrimeStar GXL SP DNA polymerase (Takara Bio RF220Q) to check exon 3 integrity and plasmid insertion using the following primers:
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bioRxiv - Microbiology 2023Quote: ... The protocol was as follows: PCR reaction mixture (100 nL) was prepared using SmartChip TB Green Gene Expression Master Mix (TaKara Bio, Japan), nuclease-free PCR-grade water ...
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bioRxiv - Genomics 2020Quote: ... and 10 μL PCR Mixture (TaKaRa, Japan). PCR reaction was conducted on a T100 thermocycler (Bio-Rad ...
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bioRxiv - Genomics 2021Quote: ... 4 ul 2.5 μM dNTP mixture (Takara), 0.8 ul 100mM DTT and 14.2 ul RNase-free water ...
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bioRxiv - Microbiology 2023Quote: ... The protocol was as follows: PCR reaction mixture (100 nL) was prepared using SmartChip TB Green Gene Expression Master Mix (Takara Bio, Shiga, Japan), nuclease-free PCR-grade water ...
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bioRxiv - Cell Biology 2020Quote: ... 100 ng of total RNA was reverse-transcribed using the PrimeScript RT Reagent Kit (Takara Bio, Shiga, Japan) and a T100 thermal cycler (Bio-Rad Laboratories ...
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bioRxiv - Genomics 2023Quote: ... 100 ng to 1 µg of total RNA was used for Hi-Mammalian whole transcriptome preparation (Takara Bio) and sequencing was performed on Nextseq2000 instrument with 1 x 72 bp single-end setup ...
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bioRxiv - Cell Biology 2022Quote: ... the cells were treated with 200 ng ml−1 of Dox (#631311; TaKaRa, Kusatsu, Japan) for 24 h ...
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bioRxiv - Developmental Biology 2022Quote: ... and the lens-specific cryaa:Cerulean marker using in-Fusion cloning (Takara Bio). We injected plasmid and Tol2 transposase RNA (5-10 ng/µL each ...
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bioRxiv - Plant Biology 2020Quote: ... cDNA was diluted to 100 ng/μl and was mixed with Green Premix Ex Taq II (Takara Bio, China) and amplifications were carried out following the manufacturer’s protocol ...
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bioRxiv - Microbiology 2023Quote: ... and an aliquot of 100 ng was reverse transcribed with the PrimeScript RT Reagent Kit with gDNA Eraser (Takara) and the included primer mix ...
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bioRxiv - Molecular Biology 2023Quote: ... 100 ng of each RNA sample was reverse-transcribed with PrimeScript RT Master Mix (Perfect Real Time) (Takara Bio). qPCR was performed using the QuantiTect SYBR Green PCR Kit (QIAGEN) ...
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bioRxiv - Microbiology 2022Quote: ... and mixture with Lenti-X concentrator (Takara Bio) according to the manufacturer’s instructions ...
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bioRxiv - Developmental Biology 2021Quote: ... and an eye-CFP selectable marker 28 using In-Fusion cloning (Takara Bio). We injected plasmids and Tol2 transposase RNA (30 ng/uL each ...
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bioRxiv - Bioengineering 2021Quote: ... 7 μL RNase-free water (Takara, Japan), and 10 μL iQ SYBR Green Supermix (Bio-Rad ...
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bioRxiv - Plant Biology 2019Quote: ... The first-strand cDNA for qRT-PCR was synthesized from 100 ng total RNA using PrimeScript RT reagent Kit with gDNA Eraser (TaKaRa). For qRT-PCR ...
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bioRxiv - Evolutionary Biology 2022Quote: ... 100 ng RNA extracted from 12 male or female pharate larvae was reverse-transcribed using PrimeScript II reverse transcriptase (TaKaRa) at 50°C for 30 min ...
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bioRxiv - Neuroscience 2023Quote: ... the RNA concentration was determined via Nanodrop and up to 100 ng was used as an input material for library preparation using the Clontech Ultra Low kit (Clontech).
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bioRxiv - Cell Biology 2023Quote: ... and varying amounts (0, 10, 20, 100, 200 ng) of pAAV MyoD expression vectors into AAVpro 293T cells (Takara Bio) using the FuGENE HD transfection reagent (Promega) ...
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bioRxiv - Immunology 2021Quote: ... the RT-RamDA mixture containing 2.5x PrimeScript Buffer (TAKARA), 0.6 μM oligo(dT)18 (Thermo) ...
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bioRxiv - Molecular Biology 2024Quote: ... a mixture containing 10μl 5x PrimeSTAR buffer (Takara, #R050B), 4μl dNTP (2.5mM ...
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bioRxiv - Animal Behavior and Cognition 2019Quote: ... Template DNA was extracted from antenna or leg of the individuals by placing them in 50 μL of 5% Chelex solution (Chelex 100, 100–200 mesh, Bio Rad) and 0.5 μL of proteinase K (20mg/mL, Takara Bio Inc.), then incubating for 24 h at 56 °C and 5 min at 95 °C ...
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bioRxiv - Developmental Biology 2020Quote: ... All samples were prepared as RNA-seq libraries with the SMARTer Stranded Total RNA-Seq Kit v2 – Pico Input (for 250 pg – 100 ng input) (Takara Bio) with RiboZero depletion ...
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bioRxiv - Genomics 2021Quote: ... The four PCR assays were run on a thermal cycler using 100 ng DNA with PrimeStar GXL PCR mastermix (Takara Bio) according to the manufacturer’s instruction using 58 °C annealing temperature ...
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bioRxiv - Evolutionary Biology 2022Quote: ... anywhere between 20 to 100 ng of template per sample was used with PrimeSTAR GXL DNA Polymerase from TAKARA (Cat: R050B). The parameters for this 1st reaction were as follows ...
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bioRxiv - Immunology 2023Quote: ... A total of 100 ng isolated RNA was used for cDNA synthesis using PrimeScript High Fidelity RT-PCR Kit (Takara, R022B). RT-qPCR was performed using TB Green® Premix Ex Taq™ (Tli RNase H Plus ...
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bioRxiv - Genetics 2023Quote: ... and selection was performed on SD-Trp-Leu plates containing 200 ng/ml of Aureobasidin A (Clontech, 630466) according to the manufacturer’s instructions (Clontech) ...