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Citations for Takara Bio :
501 - 550 of 893 citations for M CHERRY MRNA mCherry Fluorescent Protein coding mRNA since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Molecular Biology 2023Quote: ... were constructed by cloning the full-length coding region into the pGADT7 vector using the In-Fusion HD Cloning Kit (Clontech Laboratories, Mountain View, CA, USA). The Matchmaker two-hybrid system 3 (Clontech Laboratories ...
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bioRxiv - Cancer Biology 2024Quote: ... and real-time qPCR experiments were conducted using the SYBR fluorescent dye kit (TaKaRa, Japan). The primers used in this procedure included ...
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bioRxiv - Biochemistry 2019Quote: ... CoAA and hnRNP M fragments were inserted in-frame into pM vector (Clontech) containing Gal4 DNA-binding domain to produce Gal4-fusion proteins ...
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bioRxiv - Microbiology 2021Quote: ... followed by cDNA synthesis using M-MLV reverse transcriptase (TaKaRa Biotechnology, Dalian, China). The qualified cDNAs were subjected to high-throughput sequencing for viral metagenomic analysis by the Beijing Genome Institute (BGI ...
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bioRxiv - Molecular Biology 2020Quote: ... The plug was equilibrated twice in 1 ml of 1× M buffer (TaKaRa) by rotating the tube for 30 min at room temperature ...
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bioRxiv - Plant Biology 2022Quote: ... cDNA synthesis was performed using the M-MLV reverse transcriptase (Takara Bio, Inc.) according to the manufacturer’s protocol ...
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bioRxiv - Molecular Biology 2024Quote: ... The plug was equilibrated twice in 1 mL of 1× M buffer (TaKaRa) by rotating the tube for 30 min at room temperature ...
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bioRxiv - Molecular Biology 2024Quote: ... The plug was equilibrated twice in 1 mL of 1× M buffer (TaKaRa) by rotating the tube for 30 min at room temperature ...
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bioRxiv - Developmental Biology 2021Quote: DN-Brn2 expression vector was generated by PCR amplification of Brn2 DBD and addition of NES sequences using primers ATC ATC GAA TTC GAG AGT CAT GCT TCA ACT TCC TCC TCT TGA ACG CCT TAC CCT TGG AGG AGG AGG ACC GGG CCA CCC AGG CGC GCA C and GAT GAT GGA TCC CCA AGG GTA AGG CGT TCA AGA GGA GGA AGT TGA AGT CCT CCT CCT CCA CCC CCA TAC ACA TCC TCG GC and mBrn2 template plasmid (Sugitani et al. 2002) into mCherry N1 (Clontech). Subsequently ...
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bioRxiv - Cell Biology 2022Quote: ... EcoRI-BglII digestion product of pAc-GFPC1-Sec61β was ligated into pmCherry-C1 vector (made by substituting mCherry for EGFP in pEGFP-C1 (Clontech) by AgeI-XhoI digestion) ...
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bioRxiv - Molecular Biology 2020Quote: ... To generate the light-inducible clustering constructs, the CRY2olig sequence (Taslimi et al., 2014a) was inserted with a c-terminal mCherry tag (Clontech) into pGEMHE to generate pGEMHE-CRY2olig-mCherry ...
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bioRxiv - Genetics 2020Quote: ... A gblock of BtKV fused to mCherry was synthesized and inserted into the digested pET28-P2C-Cas9 using In-Fusion Cloning (Takara). Sanger sequencing was used to verify the sequence ...
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bioRxiv - Physiology 2021Quote: ... Brain sections were then incubated overnight at room temperature in blocking solution containing primary antiserum (rat anti-mCherry, Life Technologies M11217, 1:1,000; rabbit anti-dsRed, Clontech 632496 ...
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bioRxiv - Cell Biology 2021Quote: ... The sequence encoding mCherry in frame with the polybasic sequence and CAAX motif of human K-Ras (GKKKKKKSKTKCVIM) for targeting to the plasma membrane was generated by amplification of mCherry using the pmCherry-N1 (Clontech) plasmid as template and the following primers ...
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bioRxiv - Biochemistry 2022Quote: ... wild type and mutant constructs of full-length PKD1 were cloned into the EGFP-C1 and mCherry-C1 vectors (Clontech), resulting in N-terminally labelled fusion proteins ...
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bioRxiv - Microbiology 2021Quote: ... Forward and reverse primers carrying the above restriction sites were designed to amplify a 3kb fragment from Diaph2 MGC human cDNA clone (Dharmacon) and the amplicon encompassing the entire cDNA sequence minus the DAD domain was cloned into pLVX-mCherry-N1 (Clontech) to generate lentiviral expression vector pLVXdeltaDADmcherry ...
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bioRxiv - Cell Biology 2019Quote: ... We then generated by PCR a Linker+ GFP/mCherry which we inserted into the dyn2 vector with the In-Fusion HD kit (Clontech). Dyn2-GFP-ΔCter and Dyn2-GFP-ΔPRD were generated introducing a stop codon at positions 807 (after motif A ...
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bioRxiv - Genetics 2021Quote: ... This mutated fragment was replaced by the original homologous sequence of the pJet-ebony_mut-mCherry vector by fusing the mutated fragment and the PCR product amplified from the pJet-ebony-mCherry vector using the In-Fusion HD Cloning Kit (TaKaRa) with the following primers ...
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bioRxiv - Cell Biology 2019Quote: ... gBlocks® gene fragments for the following inserts were synthesized by Integrated DNA technologies (IDT) and cloned into pTRE3G-BI-mCherry (Clontech) using BamHI and NotI ...
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bioRxiv - Cell Biology 2021Quote: ... The resulting plasmid was then digested with NheI and XhoI to remove GFP and replace with mCherry from similarly digested pmCherry-C1 (Clontech).
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bioRxiv - Cell Biology 2020Quote: ... was amplified by PCR and inserted into NheI and AgeI sites of the mCherry-C1 and mCerulean3-C1 vectors (Clontech). YFP-PH(Akt ...
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bioRxiv - Neuroscience 2021Quote: ... anti-ORF1p (guinea pig, 1/200, in-house, clone 09 as in 83, anti-mCherry (mouse, 1/200, Clontech 632543), rabbit anti-H3K9me3 (rabbit ...
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bioRxiv - Developmental Biology 2021Quote: ... and cloned into into modified pJet:attB:mCherry vector (Roberts et al., 2014) containing phiC31 attB site and mCherry reporter using In-Fusion HD Cloning Kit (TaKaRa/Clontech) according to the manufacture instructions ...
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bioRxiv - Cell Biology 2021Quote: ... F-CPE-mCherry was constructed using the Gibson Chew Back and Anneal Assembly technique to subclone CPE into pmCherry-N1 (Clontech) vector using XhoI and BamHI restriction sites ...
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bioRxiv - Neuroscience 2022Quote: ... These guides were individually cloned into pAAV-U6-sasgRNA-CMV-mCherry-WPREpA at the BstXI and XhoI restriction enzyme sites using the In-Fusion HD cloning kit (Clontech). rAAV vectors were generated using similar plasmids and cloning methods as was referenced in (Matharu et al. ...
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bioRxiv - Neuroscience 2023Quote: ... pTRE3G-HA signal-Flag-GPCRs-P2A-mCherry-reverse-PGK-TetOn3G was made of Tet-ON 3G inducible expression system (Clontech) and PRESTO-Tango GPCR Kit (Kroeze et al. ...
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bioRxiv - Cell Biology 2023Quote: ... CMV-mCherry-SV40-PA was amplified via PCR and the OLIGO273 and OLIGO274 from p-mCherry-N1 without multiple cloning site (modified Clontech, Takara; United States ...
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bioRxiv - Cell Biology 2023Quote: ... CMV-mCherry-SV40-PA was amplified via PCR and the OLIGO273 and OLIGO274 from p-mCherry-N1 without multiple cloning site (modified Clontech, Takara ...
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bioRxiv - Cell Biology 2023Quote: ... Separate populations were stably-transfected to express MLCK1-EGFP constitutively and mCherry-Ig3 inducibly via a Tet-on3G system (Clontech). Cells were maintained in high-glucose Dulbecco’s modified Eagle’s medium (Life Technologies ...
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bioRxiv - Cell Biology 2023Quote: ... Expression plasmids encoding mCherry-tagged PDZ-RhoGEF and its mutants were constructed by inserting the PCR-amplified cDNA fragments into an mCherry-C1 expression plasmid (Clontech). The cDNA fragments were amplified using PrimeSTAR HS DNA polymerase (TAKARA ...
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bioRxiv - Cell Biology 2023Quote: ... or deleted of the entire netrin sequence (mCherry) were amplified by PCR followed by homologous recombination according to the inFusion deletion protocol (Clontech; dLamVI primers ...
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bioRxiv - Cell Biology 2023Quote: ... The two best performing guides from the genome-wide screen (Table S3) were selected and synthesized (IDT) and then cloned into the pLVXS-sgRNA-mCherry-hyg Vector (Takara) following the manufacturer’s instructions ...
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bioRxiv - Physiology 2024Quote: ... Tissues were then incubated in a primary antibody solution (1:500 chicken anti-GFP, abcam, and 1:500 rabbit anti-mCherry, Takara) for two days at 4°C ...
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bioRxiv - Physiology 2024Quote: ... DRGs were transferred to a primary antibody solution (1:500 chicken anti-GFP, abcam, and 1:500 rabbit anti-mCherry, Takara) overnight ...
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bioRxiv - Cell Biology 2023Quote: ... pLVXE-Blast::mCherry-TagRFP-Luciferase (RFP-cyto) was constructed by PCR and ligation of mCherry-TagRFP-Luciferase from 2xRFP-luciferase into a pLVXTight lentiviral vector (Clontech) previously modified to contain the EF1a promoter and blasticidin resistance.
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bioRxiv - Cell Biology 2020Quote: ... Protein concentrations were measured using BCA Protein Assay Kit (TaKaRa). The samples were subjected to SDS-PAGE ...
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Deletion or inhibition of PTPRO mitigates diet-induced hepatic steatosis and inflammation in obesitybioRxiv - Immunology 2022Quote: ... Protein concentrations were assessed with the BCA protein assay kit (Takara BCA Protein Assay Kit, Takara, Shiga, Japan). SDS-PAGE and Western blotting were performed as previously described (Shintani et al. ...
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bioRxiv - Cell Biology 2020Quote: ... EGFP-Zdk1-NuMA-C was cloned into the CMV promoter plasmid mCherry-Zdk1-EB1-C (van Haren et al., 2018) as follows: mCherry was replaced by EGFP amplified from pEGFP-C1 (Clonetech, Takara Bio) and EB1-C was replaced by NuMA-C1701-2115 ...
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bioRxiv - Immunology 2021Quote: ... pLVX-IRES-mCherry-mDDX18-S354L were generated by cloning the cDNA of mDDX18 or its mutants into the lentiviral vector pLVX-IRES-mCherry (TaKaRa, Japan). To package the recombinant lentiviruses ...
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bioRxiv - Neuroscience 2019Quote: ... and then incubated with a GFP antiserum (rabbit, 1:1000, Life Technology, #A6455) or an mCherry antiserum (mouse, 1:1000, Clontech, #632543). Primary antisera were diluted in PBS with 2% NGS overnight at 4°C ...
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bioRxiv - Biophysics 2019Quote: ... NG-Scarlet and NG-Cherry were created by deleting mRuby3 from NG-Ruby3 by inverse PCR and insertion of either mScarlet-I or mCherry using In-Fusion cloning (Takara Bio). NG-Stop was created using the same inverse PCR product as NG-Scarlet and NG-Cherry via blunt end ligation using NEBs KLD Mix (New England Biolabs ...
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bioRxiv - Neuroscience 2020Quote: ... and inserted into Bgl II/Sal I-linearized pL7 mGFP or pL7 mCherry (Wagner, McCroskery and Hammer, 2011) using an In-Fusion cloning kit (Takara, 638920). The specific fragments generated using the appropriate template and indicated primers were ...
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bioRxiv - Cell Biology 2021Quote: γ2 mCherry and tethered GluA2 (flop isoform)::γ260 were subcloned into the doxycycline-inducible expression vector pBI-Tet (Clontech, #6152-1) using the restriction sites MluI/XbaI and MluI/NheI ...
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bioRxiv - Cell Biology 2021Quote: ... zroraa LBD deletion DN -: 5′- ctgattatgatctagagtccaggccggattgatcagg-3 and inserted into a Tol2-lyzC-mcherry-2A backbone by using infusion cloning kit (Takara #638920). The construction method for neutrophil-specific Cas9 expression and the guide RNA expression fish lines has been described in our previous study [40] ...
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bioRxiv - Neuroscience 2019Quote: ... and then in primary antibodies in PBST at 4°C for 48 hours using rabbit anti-DsRed (mCherry tag; 1:500; Clontech; 632496), and mouse anti-Th (1:1,000 ...
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bioRxiv - Developmental Biology 2022Quote: ... This amplified fragment was named N-Cad-M and was subcloned into the 5’ side from P2A peptide (ATNFSLLKQAGDVEENPGP) of the pCAG-P2A-H2B-mCherry vector by In-Fusion Cloning (Takara, Japan). To visualize the membrane of cells that express N-Cad-M ...
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bioRxiv - Neuroscience 2022Quote: ... The full-length wild-type TRPV4 was subcloned into the PLVX-IRES-mCherry vector to generate TRPV4WT (Clontech, Catalog No. 631237). To generate the TRPV4FeRIC construct ...
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bioRxiv - Developmental Biology 2021Quote: ... and reverse-transcribed using random hexamer-primers and M-MuLV reverse transcriptase (Takara, Japan). The resulted cDNA was used to perform quantitative PCR in an Applied Biosystems Step One Plus real-time PCR system (Applied Biosystems ...
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bioRxiv - Cell Biology 2020Quote: ... the reverse transcription reactions were performed using reverse Transcriptase M-MLV (RNase H-) (TaKaRa). The cDNA was then used for real-time qPCR using the SYBR Green qPCR Master Mix (Bi-make ...
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bioRxiv - Genomics 2019Quote: ... 9.0 μL of 3 SMART™ CDS Primer II A (12 M, Clontech, 634936), and 1.4 μL of Loading Reagent (20X ...