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Citations for Takara Bio :
651 - 700 of 1209 citations for Human METTL3 shRNA Plasmid since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2022Quote: All expression plasmids are based on the lentiviral vector pLVX-EF1α-mCherry-N1 (Takara, Cat# 631986). Codon-optimized variants of GPR133 were previously published and described (Frenster et al. ...
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bioRxiv - Cancer Biology 2022Quote: ... DNA for experiments was obtained using endotoxin-free plasmid purification kits (NucleoBond Xtra Midi EF, Takara, cat ...
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bioRxiv - Cancer Biology 2022Quote: ... Recombinant AAV2 was packaged in HEK293T cells using pHelper and pRC2-mi342 plasmids (Takara, Catalog #632608). Three days after transfection ...
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bioRxiv - Molecular Biology 2020Quote: ... 2.5 μg of PrimPol variant expressing pcDNA3.1/nV5-DEST vectors11 or control plasmid pEGFP-C2 (Clontech) were transfected for the last 24 h using TransIT-2020 (Mirus Bio) ...
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bioRxiv - Synthetic Biology 2021Quote: ... Insert the purified array into the opened plasmid backbone using In-Fusion cloning (Takara Bio, Japan).
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bioRxiv - Biophysics 2021Quote: Full length mouse ILK cDNA was cloned into the EcoRI site of pEGFP-N1 plasmid (Clontech). The R255A ...
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bioRxiv - Neuroscience 2020Quote: ... PCR amplified and inserted into the DsRed-Express2 plasmid using in-fusion cloning (Takara Bio, CA). St3-Halo was generated by replacing the GFP in St3-eGFP with Halo tag (GenScript USA Inc ...
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bioRxiv - Microbiology 2021Quote: ... Plasmids were constructed using synthetic double-stranded DNA (Integrated DNA Technologies) and In-Fusion cloning (Takara) into the pL6 plasmid ...
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bioRxiv - Cell Biology 2019Quote: All plasmids were generated using standard restriction enzyme cloning and InFusion system (TaKaRa Bio, Kusatsu, Japan). The list of all plasmids used in the study is available in Table S1 ...
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bioRxiv - Cell Biology 2020Quote: ... The cloned plasmid was packaged into lentivirus using lenti-X-packaging-single-shots (VSVG) (Takara, #631275) according to the manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2021Quote: ... Deletion or substitution mutagenesis of these plasmids was performed using a PrimeSTAR Mutagenesis Basal Kit (Takara) and primers listed in Table S1 ...
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bioRxiv - Genetics 2022Quote: Full length wild-type or mutant BRC-1 sequences were cloned into plasmid pBridge (Takara Bio), transformed into yeast strain Y2HGold (Takara Bio ...
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bioRxiv - Genomics 2022Quote: ... and the bacterial lawn later scraped for plasmid extraction using NucleoBond Xtra Midi Plus (Takara Bio). The plasmid pool was then triple-digested with SphI-HF ...
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bioRxiv - Genomics 2022Quote: ... and the bacterial lawn later scraped for plasmid extraction using NucleoBond Xtra Midi Plus (Takara Bio). 20 individual bacterial colonies were separately picked from a dilution plating and miniprepped using Nucleospin Plasmid (Takara Bio) ...
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bioRxiv - Microbiology 2022Quote: ... the gene fragments of transcriptional regulators were cloned into the pColdII plasmid (Takara Bio, Kusatsu, Japan) and transformed into E ...
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bioRxiv - Genetics 2022Quote: ... 56 plasmids in 293FT HEK cells using the CalPhos™ Mammalian Transfection Kit (Takara bio, 631312). Following 2 μM tamoxifen treatment ...
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bioRxiv - Developmental Biology 2023Quote: ... This rtTA-advanced ORF was replaced with rtTA3G from the pCMV-Tet-3G plasmid (TaKaRa Bio), by XbaI and XmaI digestion and cloning of three separate fragments to create the final rtTA vector AAVS1-NEO-UBrtTA-3G.
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bioRxiv - Genomics 2023Quote: ... Recombinant AAV2 was packaged in HEK293T cells using pHelper and pRC2-mi342 plasmids (Takara, Catalog #632608). Three days after transfection ...
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bioRxiv - Microbiology 2023Quote: Retrovirus plasmids were generated by restriction enzyme cloning of latency genes into pRetroX-IRES-ZsGreen (Takara). Latency locus genes were amplified from WT MHV68-BAC DNA127 and the frame-shift stop mutant ...
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bioRxiv - Bioengineering 2023Quote: ... Plasmid DNA from the Gibson assembly reaction was then transformed into Stellar Competent Cells (Takara Bio).
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bioRxiv - Biochemistry 2023Quote: The plasmids for producing XccOpgD mutants were constructed using a PrimeSTAR mutagenesis basal kit (Takara Bio) according to the manufacturer’s instructions ...
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bioRxiv - Microbiology 2024Quote: HEK293T cells were transfected with expression plasmids using the TransIT-293 transfection reagent (Takara, Shiga, Japan), following the manufacturer’s instructions.
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bioRxiv - Molecular Biology 2024Quote: ... YCplac22 plasmids that carry tsa1 mutant alleles were constructed using the PrimeSTAR Mutagenesis Basal Kit (TaKaRa) according to the manufacturer’s instructions ...
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bioRxiv - Developmental Biology 2024Quote: ... Col2a1a enhancer was amplified from the plasmid -1.7kbcol2a1a:EGFP-CAAX (24) using CloneAmp HiFi premix (Takara #639298) with the primers ...
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bioRxiv - Immunology 2024Quote: ... Plasmid sequences confirmed by Sanger sequencing (Quintara Biosciences) were transformed into Stellar Competent Cells (Takara Bio) and purified using the NucleoBond Xtra Midi endotoxin-free midi-prep kit (Takara Bio) ...
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bioRxiv - Cancer Biology 2019Quote: ... A full-length Myc tagged cDNA expressing human NEK9 (NM_001329237.1) was subcloned into the pVLX-Tight-Puro vector (Clontech). The RCC1 domain was deleted using the Quickchange® II XL Site-Directed Mutagenesis Kit according to manufacturer’s instructions (Stratagene ...
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bioRxiv - Cell Biology 2022Quote: ... a DNA fragment was PCR-amplified from human genomic DNA using Tks Gflex DNA Polymerase (#R060A, Takara Bio) and the primers listed in Table S1 ...
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bioRxiv - Cell Biology 2023Quote: ... Full-length human CLUH and mouse Cluh coding sequences cDNA were cloned in pcDNA3 and pmCherry-N1 (Clontech), respectively ...
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bioRxiv - Biochemistry 2020Quote: ... pGAD plasmids were transformed into PJ49-4a yeast and were selected on leucine-dropout medium (Clontech, #630414). pTEF or pTEFdual plasmids were included to express proteins in trans and were transformed with pOBD plasmids and selected on media lacking tryptophan and uracil (Sunrise Science Products ...
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bioRxiv - Evolutionary Biology 2021Quote: ... All RNA clones were prepared from the plasmids by in vitro transcription with T7 RNA polymerase (Takara) after digestion with Sma I (Takara) ...
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bioRxiv - Molecular Biology 2020Quote: ... The 3xFlag sequence was then ligated into the digested eGFP-Usp9x plasmid (Takara DNA ligation kit #6023).
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bioRxiv - Cancer Biology 2019Quote: ... BirA(R118G)-HA from pcDNA3.1 MCS-BirA(R118G)-HA plasmid was cloned into pRetroX-Tight-Pur (Clontech) at EcoRI site by PCR to get pRetroX-MCS-BioID-HA vector ...
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bioRxiv - Microbiology 2019Quote: ... Subsequently each guide was inserted into the BtgZI linearized pCas/sg plasmid by In-Fusion cloning (Takara) using primers fwd-TTACAGTATATTATT(N20)GTTTTAGAGCTAGAA and rev-TTCTAGCTCTAAAAC(N20)AATAATATACTGTAA ...
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bioRxiv - Cell Biology 2019Quote: The plasmid pDendra2NatMX was constructed by ligation of the NheI-HpaI Dendra2 fragment from pDendra2-C (Clontech) and the PvuII linearized pAG25 vector (Addgene) ...
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bioRxiv - Biophysics 2019Quote: ... The mEos3.2 tagged constructs used here are in Clontech N1 plasmid vector background (Clontech, Mountain View, CA).
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bioRxiv - Evolutionary Biology 2021Quote: ... pre-amplified (DFRS empty plasmid primers) and pre-digested by the same enzymes (In Fusion Kit; Clontech). The miR-137 or miR-122 complementary sequences were thereby located on the 3’UTR of the RFP gene.
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bioRxiv - Biochemistry 2020Quote: ... Proteins were expressed in Escherichia coli strain BL21(DE3) cells containing chaperone plasmid pG-KJE8 (TAKARA, 3340). When the optical density at 600 nm reached 0.6 ...
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bioRxiv - Cell Biology 2021Quote: ... Slc37a2 was amplified by PCR from BMMCs cDNA and inserted into a plEGFP-C1 plasmid (Takara Bio). Slc37a2 plEGFP-C1 plasmid or plasmid vector control were transfected into Amphopack-293 cells (BD Biosciences ...
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bioRxiv - Neuroscience 2020Quote: ... This sequence was inserted into a donor plasmid (pCRII-TOPO) using In-Fusion HD cloning (Takara – 639649). For the Cas9 plasmid ...
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bioRxiv - Microbiology 2020Quote: ... cerevisae: All Yeast-two hybrid plasmids were generated on the basis of the Matchmaker III System (Clontech Laboratories Inc. ...
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bioRxiv - Biochemistry 2022Quote: ... coding regions were cloned downstream of the CMV promoter in derivatives of the pEGFP-N1 plasmid (Clontech) with an NLS and linker sequences to EGFP or mCherry (Supplemental Table 2) ...
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bioRxiv - Molecular Biology 2022Quote: ... The plasmid DNA bearing the chaperone combination GroEL/ES (pGro7) or DnaK/J/GrpE (pKJE) from Takara Biotech chaperone kit [Nishihara et al. ...
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bioRxiv - Cell Biology 2022Quote: ... Immortalization of the cells was achieved by lipofection of the pCDNA-3xHA-hTERT plasmid with Xfect (Takara BIO INC ...
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bioRxiv - Neuroscience 2020Quote: ... The knock-in plasmid was constructed by using In-Fusion HD Cloning kit (Takara Bio USA #639650) or NEBuilder HiFi DNA Assembly kit (New England Biolabs #M5520 ...
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bioRxiv - Molecular Biology 2020Quote: ... and ligated into the Nhe1 site of the pBS32-Flag-MS2CP parent plasmid using InFusion cloning (Clontech). The PTBP1 Y247Q ...
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bioRxiv - Bioengineering 2021Quote: ... an endotoxin-free plasmid midiprep DNA purification took place using NucleoBond Xtra Midi EF kit (Takara Bio) following the manufacturer’s protocol ...
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bioRxiv - Microbiology 2021Quote: ... The sequence encoding amino acids 2-1273 were cloned into a shuttle plasmid following InFusion cloning (Clontech). The shuttle plasmid encodes a modified human cytomegalovirus major immediate early promoter (IE CMV ...
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bioRxiv - Pharmacology and Toxicology 2022Quote: ... was used to transfect cells at 70% confluency with 20 ng of CRE-SEAP reporter plasmid (Clontech) and 20 ng of receptor or empty vector pcDNA-Zeo-tetO DNA per well ...
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bioRxiv - Bioengineering 2020Quote: ... Plasmid assembling steps were performed with the In-Fusion HD Cloning Kit (Clontech, Mountain View, CA, USA). The gene cassettes used for the cell-surface expression of hemicellulases were previously optimized [43 ...
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bioRxiv - Molecular Biology 2021Quote: All PCR amplification and insertion into plasmids were performed with PrimeSTAR MAX and In-Fusion HD (TaKaRa), respectively.