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Citations for Takara Bio :
601 - 650 of 2435 citations for Human Chemokine C X C Motif Receptor 5 CXCR5 Protein since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2021Quote: ... Additional to the NF-κB binding motifs NF-κB response element and a TATA-like sequence from pNFκB-Luc vector (Clontech Cat. No. 631904) were added to the Firefly luciferase gene and introduced into pCDH-CMV-EF1-Puro.
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bioRxiv - Bioengineering 2021Quote: ... Human umbilical vein endothelial cells (HUVECs) and human aortic smooth muscle cells (HAoSMCs) were purchased from TAKARA BIO (Tokyo ...
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bioRxiv - Pharmacology and Toxicology 2022Quote: Site-directed mutagenesis in GC-A and GC-B and construction of chimeric receptors were performed through use of the In-Fusion HD plus cloning kit (Takara Bio Inc.). The primers used are listed in Supplementary Table 1 ...
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bioRxiv - Molecular Biology 2022Quote: ... The amplified fragments required to generate the chimeric receptor were combined with a linearized pCS2+ backbone and annealed using an In-Fusion kit (Takara Bio, 638947). The resulting plasmids were transformed into Top10 chemically competent cells ...
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bioRxiv - Immunology 2023Quote: ... Plasmids were packed into lentiviral particles using the Lenti-X cell line and Lenti-X Packaging Single Shots (VSV-G) (Takara, Cat.No. 631276) according to manufacturer’s protocol ...
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bioRxiv - Neuroscience 2020Quote: ... 341F (5’-CCTACGGGNGGCWGCAG-3’) and 805R (5’-GACTACHVGGGTATCTAATCC-3’) or (2) 341F (5’-TCGTCGGCAGCGTCAGATGTGTATAAGAGACAGCCTACGGGNGGCWGCAG-3’) and 806R (5’-GTCTCGTGGGCTCGGAGATGTGTATAAGAGACAGGGACTACHVGGGTWTCTAAT-3’) (TaKaRa Bio, Shiga, Japan). The second PCR was performed to add the index sequences for Illumina sequencing with barcode sequences ...
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bioRxiv - Neuroscience 2019Quote: ... test genes (vascular connexins) in human vessels were normalized relative to human whole heart (RNA purchased from Clontech) and in mouse ...
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bioRxiv - Neuroscience 2020Quote: ... cDNA of Human Phosphodiesterase type 10 (hPDE10) was amplified by conventional nested PCR using human brain cDNA (Clontech). cDNAs template encoding wild-type and D674A mutant form of hPDE10 catalytic domain (CD ...
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bioRxiv - Developmental Biology 2024Quote: ... Human full-coding KATNA1 cDNA was isolated from a human fetal brain Marathon-ready cDNA collection (Clontech, Invitrogen) with the following forward and reverse primers ...
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bioRxiv - Microbiology 2020Quote: ... The virus was concentrated using Lenti-X Concentrator (Clontech). Lentivirus titers from 48h and 72h were determined by plaque assay (data not shown ...
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bioRxiv - Neuroscience 2021Quote: ... We used Lenti-X GoStix Plus (Takara Bio USA) to quantify the titer of each virus batch used in this study ...
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bioRxiv - Neuroscience 2021Quote: 1 part of Lenti-X™ concentrator (TaKaRa; 631232) was first mixed with 3 parts of supernatant and incubated at 4 °C overnight for lentivirus precipitation ...
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bioRxiv - Molecular Biology 2021Quote: Lentiviral production was confirmed with Lenti-X GoStix (Takara) and lentiviruses were concentrated with Lenti-X Concentrator (Takara ...
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bioRxiv - Molecular Biology 2021Quote: ... and lentiviruses were concentrated with Lenti-X Concentrator (Takara) according to the manufacturer’s recommendations ...
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bioRxiv - Immunology 2019Quote: ... and virus was concentrated using Lenti-X Concentrator (Takara). Existing mIRAK1mCherry and Irak1−/− iBMDM were then transduced with concentrated lentivirus ...
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bioRxiv - Physiology 2020Quote: ... and titered with Adeno-X Rapid Titer Kit (Clontech). Adenoviruses were delivered into mice intravenously at 5 × 108 pfu/mouse for overexpression experiments ...
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bioRxiv - Genomics 2019Quote: ... Lentivirus was concentrated using the Lenti-X Concentrator (Takara). mHypoA-POMC/GFP-1 cells were infected with sgRNA and KRAB-dCas9 virus (1:1 ratio ...
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bioRxiv - Developmental Biology 2021Quote: ... was concentrated using Lenti-X™ Concentrator (631232, Takara). Lentivirus pellets were dissolved in 400 µl PBS.
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bioRxiv - Biochemistry 2021Quote: ... Lenti-X p24 rapid titre kit (Takara Bio, JP) was used to quantify the viral titres ...
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bioRxiv - Bioengineering 2021Quote: ... Lenti-X 293T cell line was purchased from Takara Bio USA Inc ...
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bioRxiv - Neuroscience 2020Quote: ... and then concentrated with Lenti-X concentrator (Clontech, 631231), before being snap frozen in liquid nitrogen ...
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bioRxiv - Cancer Biology 2021Quote: ... packaging plamid transfected Lenti-X 293T cells (TaKaRa, #632180) using Lipofectamine 3000 (ThermoFisher Scientific) ...
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bioRxiv - Plant Biology 2020Quote: ... Ade and His but containing X-α-gal (Clontech) and 10 mM 3-amino-1,2,4-triazole (3-AT) ...
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bioRxiv - Cell Biology 2021Quote: ... and concentrated using Lenti-X (Takara Biosciences, Catalog #631232) via the standard protocol ...
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bioRxiv - Cell Biology 2020Quote: ... Viral particles were precipitated using Retro-X Concentrator (Clontech) and resuspended in STEMdiff Neural Progenitor Medium containing 8 μg/ml Polybrene (EMD Millipore) ...
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bioRxiv - Cell Biology 2020Quote: ... and concentrated with a Lenti-X™ Concentrator (TAKARA). Titers (~1×108 infectious units/ml ...
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bioRxiv - Cell Biology 2020Quote: ... and concentrated using Lenti-X Concentrator (Takara Bio 631231). Lentiviral pellets were resuspended in DMEM ...
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bioRxiv - Immunology 2022Quote: ... Vero E6-TMPRSS258 and Lenti-X 293T cells (Takara) were cultured in Dulbecco’s Modified Eagle’s medium (DMEM) ...
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bioRxiv - Cell Biology 2022Quote: ... Lentivirus was concentrated using the Lenti-X concentrator (TaKaRa). Cells were selected for six days in puromycin followed by three days induction with doxycycline ...
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bioRxiv - Biophysics 2022Quote: ... HEK-D (see below) and Lenti-X 293T (Takara) cells were cultured in growth medium consisting of Dulbecco’s modified Eagle’s medium (GIBCO) ...
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bioRxiv - Cell Biology 2022Quote: ... and concentrated 40-fold with Lenti-X Concentrator (Takara). The concentrated lentivirus was used immediately or stored at −80°C ...
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bioRxiv - Microbiology 2020Quote: ... and mixed with the Lenti-X Concentrator (Takara, 631231) overnight at 4 °C ...
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bioRxiv - Neuroscience 2021Quote: ... and combined with Lenti-X concentrator (Takara, cat# 631232). The mixture was incubated over night at 4°C ...
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bioRxiv - Neuroscience 2020Quote: ... lentivirus was concentrated 10x using lenti-X concentrator (Clontech) with the concentrated pellet being re-suspended in PBS with 25 mM HEPES ...
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bioRxiv - Neuroscience 2021Quote: ... The virus was concentrated using Lenti-X Concentrator (Clontech), and suspended in PBS.
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bioRxiv - Bioengineering 2020Quote: ... Lenti-X™ qRT-PCR Titration Kit (Takara biosciences) was used to calculate the viral multiplicity of infection (MOI ...
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bioRxiv - Cancer Biology 2022Quote: ... concentrated using Lenti-X™ Concentrator (TaKaRa, Cat. #631232), and stored in aliquots at −80 °C ...
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bioRxiv - Immunology 2019Quote: ... we co-transfected 293T Lenti-X cells (Clontech laboratories) with plasmids expressing VSV-G ...
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bioRxiv - Cell Biology 2020Quote: ... the pRetro-X vector system (Clontech, Mountainview, CA, USA) was used ...
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bioRxiv - Immunology 2020Quote: ... Lenti-X 293T packaging cells were obtained from Takara Bio (Japan) ...
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bioRxiv - Molecular Biology 2021Quote: ... The Lenti-X 293T cells were purchased from Clontech Laboratories (632180 ...
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bioRxiv - Microbiology 2019Quote: ... Virus packaging was assessed using Lenti-X GoStix (Clontech) but virus titers were not otherwise measured ...
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bioRxiv - Cancer Biology 2019Quote: ... the lentivirus was concentrated using Lenti-X concentrator (Takara) according to the manufacturer’s protocol ...
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bioRxiv - Genomics 2021Quote: ... and concentrated by Lenti-X concentrator (Takara Bio, 631232). Titration of the lentiMPRA library was conducted on hESCs as described previously (Gordon et al. ...
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bioRxiv - Cell Biology 2020Quote: Lenti-X™ p24 Rapid Titration ELISA Kit (TaKaRa) was used to determine virus titer ...
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bioRxiv - Microbiology 2022Quote: ... Viruses were concentrated using the Lenti-X concentrator (Takara) according to the manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2021Quote: ... Virus was extracted using Lenti-X concentrator (Clontech Takara) according to the manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2021Quote: ... Virus was extracted using Lenti-X concentrator (Clontech Takara) according to the manufacturer’s instructions ...
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bioRxiv - Microbiology 2020Quote: ... using Lenti-X high-titer lentiviral packaging systems (Clontech). The cell culture supernatant was harvested after 48 h and 72 h ...
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bioRxiv - Immunology 2021Quote: ... followed by concentrating with Lenti-X concentrator (Takara, 631231). The viral supernatants were transduced into Treg cells that were expanded for 1 day before the transduction ...