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Citations for Takara Bio :
51 - 100 of 336 citations for Dl 4 Methoxyestradiol 13 14 15 16 17 18 13C6 99% since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2023Quote: ... Supernatants from rescue plates were passaged on 15 cm plates of HEK 293T/17 cells (ATCC) transfected with pLV-TTBG and pLV-TTBL (described above) using Xfect transfection reagent (Takara 631318) according to the manufacturer’s protocol ...
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bioRxiv - Microbiology 2023Quote: ... as previously described (17): pcDNA3.1+-based plasmids used for ectopic expression and pRetroX-tight-Puro-based ones (Clontech, cat. PT3960-5) used here to generate stable cell lines expressing ISG20 upon induction with doxycycline (dox.) ...
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bioRxiv - Plant Biology 2022Quote: ... 1 μg total RNA was used for cDNA synthesis by oligo(dT)18 primer according to the manufacturer’s protocol (Takara). The resulting cDNA was subjected to relative quantitative PCR using the ChamQ™ SYBR qPCR Master Mix (Vazyme Biotech Co.,Ltd ...
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bioRxiv - Neuroscience 2023Quote: ... differentiated HUES9 hESCs (Passage 12-18; RRID:CVCL_0057) were plated on 12-well iMatrix-511 (Cat.No. T303; Takara Bio Inc) coated plates at a density of approximately 3 x 106 cells/well in Synaptojuice medium (adapted from 124 ...
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bioRxiv - Microbiology 2020Quote: ... Underlined bases indicate 15-bp for In-Fusion cloning (Clontech). The amplified DNA fragment was inserted into the NdeI and XhoI site of pET23b(+ ...
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bioRxiv - Molecular Biology 2022Quote: ... Five microliters of DNase I (15 K units, TaKaRa Bio), 0.2 U/μL of ribonuclease inhibitor (porcine liver ...
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bioRxiv - Biophysics 2024Quote: ... Lentivirus was concentrated 15-fold using LentiX concentrator reagent (Takara), resuspended in PBS ...
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bioRxiv - Neuroscience 2020Quote: Total RNA was extracted from tissues of 13-month-old wildtype C57BL/6J mice (n=3) using RNAiso Plus (Takara Bio). Complementary DNA (cDNA ...
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bioRxiv - Evolutionary Biology 2021Quote: Amplified with V4 16S rRNA primers as above using EmeraldAmp GT PCR Master Mix (TAKARA BIO).
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bioRxiv - Developmental Biology 2020Quote: ... Pegl-17::GFP1-10 constructs were generated by cloning egl-17 promotor and GFP1-10 sequences into pPD95.77 plasmids using In-Fusion Advantage PCR cloning kit (Clontech, cat. no. 639621). The plasmid was injected into SPC-1::GFP11×7 knock-in animals ...
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bioRxiv - Molecular Biology 2022Quote: ... These sequences were obtained from pPIGA3GFP (inverted repeats of piggyBac transposon [17]) and pAcGFP-Hyg C1 vector (CMVp-AcGFP and SV40p-Hyg) (632492; Takara Bio USA). Recombinant baculovirus was then produced using a Bac-to-Bac system ...
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bioRxiv - Molecular Biology 2019Quote: ... The specific PCR products were gel purified by using the DNA Gel Extraction Kit (Axygen, USA) and cloned to the pMD-18 vector system (Takara), and then sequenced by the Shanghai Sangon Company.
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bioRxiv - Microbiology 2021Quote: ... The parasite load in the livers and HepG2 cells was evaluated by Taqman-PCR with primers and probes for 18S rRNA and GAPDH following the manufacturer’s instructions of Premix Ex Taq™ (Probe qPCR) (TAKARA). For the SYBR quantitative PCR assay ...
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bioRxiv - Molecular Biology 2020Quote: ... 9-15 cycles of PCR amplification were performed with Extaq (Takara). Finally ...
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bioRxiv - Synthetic Biology 2020Quote: ... A middle-copy (15-30 copies) episomal plasmid pGADT7 (Takara Bio) was used to increase the concentration of synthetic circuit components in order to buffer for the intrinsic molecular noise ...
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bioRxiv - Immunology 2021Quote: ... were incubated with 15 μL of Ni-NTA agarose beads (Takara) overnight at 4°C ...
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bioRxiv - Molecular Biology 2020Quote: ... 9-15 cycles of PCR amplification were performed with Extaq (Takara). Finally ...
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bioRxiv - Genomics 2020Quote: ... followed by 15 cycles of PCR using Takara LA Taq (Clontech) was used ...
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bioRxiv - Neuroscience 2020Quote: ... oligo(dT)-enriched RNA underwent 14 cycles of amplification using the SMARTer Ultra Low RNA Kit for Illumina Sequencing (Clontech). After cDNA fragmentation ...
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bioRxiv - Neuroscience 2021Quote: ... AP-MDGA1 and AP-MDGA2 were generated by replacing the V5 tag of the V5-MDGA1 and V5-MDGA2 constructs respectively by the 14 amino acids AP tag (5’GGCCTGAACGAtATCTTCGAGGCCCAG AAGATCGAGTGGCACGAG3’) using the HD-In-Fusion kit (Takara). The linker 5’GGAGGATCAGGAGGATCA3’ was added after the AP tag ...
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bioRxiv - Plant Biology 2022Quote: ... nls-edCitrineT9 was amplified from nlsABACUS1-2μ plasmid 14 and introduced into the pENTR221-f1-ABA-CUS2-truncation vectors using In-Fusion cloning (Takara) to generate pENTR-nlsABACUS2-100n and pENTR-nlsABACUS2-400n ...
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bioRxiv - Neuroscience 2023Quote: ... A total of 14 PCR cycles of amplification was performed for each sample using PrimeSTAR GXL DNA Polymerase (Clontech, UK). Library preparation of the amplified cDNA products was then performed using SMRTbell Template Prep Kit v1.0 (PacBio ...
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bioRxiv - Neuroscience 2020Quote: Ten 15-cm dishes of sub-confluent Lenti-X 293T cells (Clontech) were used for each purification ...
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bioRxiv - Plant Biology 2019Quote: ... 60 °C for 20 s and 72 °C for 15 s (Clontech SYBR Green Master Mix and Mx3000P qPCR Systems ...
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bioRxiv - Immunology 2023Quote: ... in 6-well plates pre-coated with 15 ug retronectin (Takara T100A). Plates were spinfected by centrifugation at 2500 rpm 32C for 90 minutes ...
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bioRxiv - Cell Biology 2021Quote: Previously described MEFs-IRE1-mNG and U2OS-IRE1-mNG cell lines (14, 27) were cultured in high glucose DMEM media supplemented with 10% tetracycline-free fetal bovine serum (FBS; Takara Bio), 6 mM L-glutamine ...
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bioRxiv - Neuroscience 2023Quote: ... expressing UAS-eGFP was subjected to 14 cycles of PCR amplification (SMARTer Seq V4 Ultra Low Input RNA Kit; Takara Bio). 1 ng of amplified RNA was used to prepare cDNA libraries (Nextera XT DNA library preparation kit ...
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bioRxiv - Molecular Biology 2021Quote: ... under high mutation rate conditions (9-16 mutations per kilobase pair) and subcloned into the pN1 vector (Clontech). Obtained gene libraries in expression vectors were electroporated into NEB10-β E.coli host cells (New England BioLabs) ...
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bioRxiv - Microbiology 2023Quote: The 16S rRNA gene sequences were amplified by PCR using LA Taq polymerase (TaKaRa-Bio, Inc., Shiga, Japan) using the primers Uni530F and Uni907R65 ...
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bioRxiv - Microbiology 2023Quote: ... ISG20 expression was routinely induced upon incubation of cells for 18 hours with 1.5 μg/mL of doxycycline (Takara Bio, cat. 631311).
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bioRxiv - Genetics 2024Quote: Two μg of total RNA was reverse transcribed into cDNA templates using RNA to cDNA EcoDry™ Premix kit including both random hexamer and oligo(dT)18 primers (Takara Bio, 639548). KAPA SYBR® FAST qPCR Master Mix (2X ...
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bioRxiv - Plant Biology 2022Quote: ... the membranes were stripped for 15 minutes with Western BLoT Stripping Buffer (Takara) following the manufacturer’s instructions and reprobed with Ubiquitin11 (Agrisera AS08307 ...
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bioRxiv - Evolutionary Biology 2022Quote: ... The 16S rRNA V4 region was PCR-amplified with buffer and polymerase PrimeSTAR GXL DNA Polymerase (Takara, Cat: R050B). The cycling conditions for the PCR are as follows:_98 °C for 3 min ...
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bioRxiv - Immunology 2020Quote: ... Cell supernatants were collected at 24 and 48 h after transfection of HEK 293/17 cells and concentrated using a LentiX concentrator (Takara Bio USA, Inc., Mountain View, CA). HEK 293/17 cells or NSC cells were plated in 6 well plates at a density of 105 per well ...
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bioRxiv - Neuroscience 2020Quote: ... 4 U RNAse inhibitor (Takara, 2313A), 10mM dNTPs (Thermo Scientific ...
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bioRxiv - Molecular Biology 2023Quote: ... The recombinant AAV2 genome rAAVeCFPrep was constructed by replacing the mCherry coding sequence in the plasmid pAAVtCR (18) with the ECFP coding sequence from pECFP (Clontech, Mountain View, CA, USA). Purified recombinant AAV2 vectors ...
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bioRxiv - Microbiology 2020Quote: ... The proteins were induced by 200 mM IPTG overnight at 16°C to generate soluble forms and purified with TALON metal affinity resin (635501, Clontech). The proteins were eluted with 250 mM imidazole and subsequently dialyzed in PBS buffer (pH 7.4) ...
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bioRxiv - Microbiology 2022Quote: ... 16S rRNA gene sequences were amplified by polymerase chain reaction (PCR) using LA Taq polymerase (TaKaRa-Bio, Inc., Kusatsu, Japan) for Illumina MiSeq paired-end sequencing ...
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bioRxiv - Systems Biology 2022Quote: ... Optimized coding sequences were synthesized as gBlocks (Integrated DNA Technologies) carrying 16-base pair overhangs at the 5’ and 3’ ends to facilitate in-fusion cloning (Clontech) into pET expression vectors (EMD MIllipore).
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bioRxiv - Developmental Biology 2020Quote: ... The presence of bacterial DNA was detected and quantified via amplification of the 16S rRNA bacterial gene and the ribosomal mosquito gene S17 via qPCR using the SYBR qPCR Premix Ex Taq (Takara) in a Light Cycler 480 (Roche) ...
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bioRxiv - Cancer Biology 2024Quote: ... with psPAX2 and pMD2.G viral particles harvested during the 16-72 h window post-transfection were concentrated with Lenti-X (Clontech) and stored at -80°C ...
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bioRxiv - Microbiology 2021Quote: ... The fragmented RNA was treated with 15 U of CIAP (Takara Bio, Otsu, Japan) at 37°C for 30 min ...
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bioRxiv - Molecular Biology 2019Quote: ... 15 pmol of siRNA is reverse transfected to 7×104 HeLa-Tetoff cells (Clontech) in a 12 well plate using 1.6 µl RNAiMAX (Thermo Fisher Scientific ...
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bioRxiv - Cell Biology 2020Quote: ... 15 nucleotide overlaps were included in primers for infusion cloning (Clontech, Mountain View, CA). Where this failed ...
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bioRxiv - Zoology 2022Quote: ... and 2X KAPA HiFi Hot Start Ready Mix 15 μl (TaKaRa Bio Inc., Japan), via a procedure with a thermal instrument (Applied Biosystems 9700 ...
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bioRxiv - Systems Biology 2022Quote: ... and 2X KAPA HiFi Hot Start Ready Mix 15 μl (TaKaRa Bio Inc., Japan), via a two-stage PCR procedure with a thermal instrument (Applied Biosystems 9700 ...
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bioRxiv - Biochemistry 2022Quote: ... one microgram of total RNA was employed to obtain the corresponding cDNA target sequences using an oligo(dT)18 primer and the PrimeScript RT reagent kit (Perfect Real Time, Takara Bio Inc., Otsu, Shiga, Japan), following the manufacturer’s directions ...
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bioRxiv - Microbiology 2022Quote: ... into which all possible combinations of 36 assays and 144 samples (127 woodchip samples, 16 standards, and one no-template control) were loaded by the SmartChip MultiSample NanoDispenser (Takara Bio). In brief ...
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bioRxiv - Microbiology 2023Quote: 0.35×105 293TT cells per plate were plated in 24-well plates 16-20 h prior to transfection of plasmids encoding HA-tagged CA Rab9a or the empty vector as control (Takara, 632105). 48 h after transfection ...
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bioRxiv - Genomics 2024Quote: ... The polymerase chain reaction (PCR) was performed on genomic DNA to amplify 16s rRNA using Emerald Amp MAX PCR Master Mix (Takara Bio) using the primers presented in Table S1.