Labshake search
Citations for Takara Bio :
1 - 50 of 336 citations for Dl 4 Methoxyestradiol 13 14 15 16 17 18 13C6 99% since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Genomics 2022Quote: ... and post-capture PCR amplification was performed for 16-18 cycles using PrimeSTAR GXL Polymerase (Takara) at an annealing temperature of 60°C.
-
bioRxiv - Genomics 2022Quote: ... prior to another round of PCR amplification for 16-18 cycles using either LA-Taq or PrimeSTAR GXL (Takara) at an annealing temperature of 60℃.
-
bioRxiv - Genomics 2021Quote: ... Libraries from 4-cell embryos were also prepared using 18-30 nt gel purified RNA using the SMARTer smRNA-Seq Kit (Clontech) and NEXTflex-Small-RNA-Seq (New England Biolabs ...
-
bioRxiv - Cell Biology 2022Quote: ... and oligo(T)18 primers (Takara). Relative transcript amounts were measured by SYBR-green quantitative PCR (qPCR ...
-
ARL3 Mediates BBSome Ciliary Turnover by Promoting Its Outward Diffusion through the Transition ZonebioRxiv - Cell Biology 2021Quote: ... and oligo(T)18 primers (Takara). ARL3 cDNA and cDNAs encoding the six BBS proteins BBS1 ...
-
bioRxiv - Molecular Biology 2019Quote: ... Same volume of samples were loaded on 4-16% gradient SDS-PAGE gels and analyzed by western blot analysis using EGFP (JL-8; Clontech), penta-HIS (QIAGEN ...
-
bioRxiv - Microbiology 2020Quote: ... Different amounts of total RNA (4, 16, 32, or 40 ng) were used for first strand synthesis using SmartScribe RT (Clontech) and Oligo(dT)23 VN primer (NEB) ...
-
bioRxiv - Cancer Biology 2019Quote: ... which was a gift from Joan Massagué (Addgene plasmid # 58263)18 and contains the pLVX-IRES-Hygro backbone (Clontech). The FADD-DD insert was replaced by subcloning the multiple cloning site of the pLVX-Puro backbone plasmid via SnaBI and BamHI restriction ...
-
bioRxiv - Molecular Biology 2023Quote: ... 18 µl of Stellar (Takara Bio Europe SAS 636766) chemically competent bacteria were transformed using 2 µl of the ligation reaction ...
-
bioRxiv - Cancer Biology 2020Quote: ... Day 17 embrionic RNA was used to validate primers (Clontech/Takara). cDNA was synthesised with Superscript III (Invitrogen) ...
-
bioRxiv - Cancer Biology 2020Quote: ... Day 17 embrionic RNA was used to validate primers (Clontech/Takara). cDNA was synthesised with Superscript III (Invitrogen) ...
-
bioRxiv - Genetics 2020Quote: ... The beads were then suspended in TdT reaction buffer (1× NEBuffer #4, 0.25 mM CoCl2, 15 U TdT (Takara Bio), 20 Ci α-32P-dCTP [6000 Ci/mmol]) ...
-
bioRxiv - Cancer Biology 2023Quote: ... and purified cDNA was eluted in 17 µL elution buffer provided by Clontech. All samples were quantitated using Bioanalyzer 2100 Instruments (Agilent Genomics) ...
-
bioRxiv - Neuroscience 2023Quote: ... and purified cDNA was eluted in 17 μl elution buffer provided by Takara. All samples were quantitated using PicoGreen® on Molecular Dynamics M2 SpectraMax instrument ...
-
bioRxiv - Neuroscience 2021Quote: ... Hippocampal neurons were transfected on DIV11-13 using CalPhos Mammalian Transfection kit (Takara) per manufacturer’s instructions ...
-
bioRxiv - Microbiology 2019Quote: ... PCR products were cloned into the pMD 18-T vector (Takara, China) at BamH I and Hind III sites ...
-
bioRxiv - Microbiology 2020Quote: ... the V3-V4 regions of the 16S rRNA gene were amplified using a 16S (V3–V4) Metagenomic Library Construction Kit for NGS (Takara Bio Inc, Kusatsu, Japan). The following primers were used (16S rRNA gene-specific sequences are underlined) ...
-
bioRxiv - Pathology 2019Quote: ... Corresponding cDNA was obtained using reverse transcriptase and Oligo (dT) 18 primer (TaKaRa). An aliquot of the cDNA was mixed with 25 µL SYBR® Green PCR Master Mix (TaKaRa ...
-
bioRxiv - Neuroscience 2023Quote: ... DCX: doublecortin (C-18) goat polyclonal IgG (1:200, Takara Bio, Cat# 632496). NeuN ...
-
bioRxiv - Cancer Biology 2022Quote: SV40-MES-13 cells were lysed with xTractor Buffer Kit (CLONTECH Laboratories, Inc., CA, USA). Protein specimens were quantified through BCA method and electrophoresed through 10% SDS-PAGE gels ...
-
bioRxiv - Neuroscience 2019Quote: ... 10-15 µg of EGFP (Clontech) was coated onto 6-8 mg of 1.6 µm gold beads.
-
bioRxiv - Genomics 2019Quote: ... we used the miRNA quantifications from all brain libraries with all starting amounts using both batches (total of 99 libraries, 19 for the Clontech, NEXTflex ...
-
bioRxiv - Genomics 2022Quote: ... 16 µL 100x DAPI and 8 µL ICELL8 Second Diluent (Takara) were added and incubated 10 minutes at RT ...
-
bioRxiv - Biochemistry 2022Quote: ... varieornatus [16] and inserted into pAcGFP1-N1 or pAcGFP1-C1 (Clontech) with (GGGGS)3 linker using In-Fusion HD Cloning Kit (Takara) ...
-
bioRxiv - Microbiology 2020Quote: ... A 16 μg/ml solution of Retronectin (T100B; Takara Bio Inc.) was prepared in PBS and stored at −20 °C ...
-
bioRxiv - Molecular Biology 2023Quote: ... 14-3-3γ CDS was amplified by PCR and subcloned into pCMV-mCherry (Clontech). Deletion and point mutations of SMAUG1 were created using Q5 site-directed mutagenesis kit (NEB) ...
-
bioRxiv - Cell Biology 2023Quote: ... 14-3-3τ and β-actin were designed and synthesized by Takara (Table 1). To run the real-time PCR reaction ...
-
bioRxiv - Microbiology 2021Quote: ... using 15 μl of elution buffer (Takara). Library quantification was performed with a Qubit 3 Fluorometer with dsDNA Hs Assay kit (Life Technologies ...
-
bioRxiv - Biochemistry 2021Quote: ... After 16 hours of incubation with B/B homodimerizer (Takara Bio, 635059) at various concentrations ...
-
bioRxiv - Neuroscience 2020Quote: Primary dissociated hippocampal neurons were transfected at 14 DIV using CalPhos mammalian transfection kit (Clontech) as previously described in Jiang and Chen ...
-
bioRxiv - Molecular Biology 2023Quote: ... and fragments were cloned into the pSAM2-Puro lentiviral vector 13 by In-Fusion HD Cloning (Takara).
-
bioRxiv - Molecular Biology 2022Quote: ... was used for oligo(dT)18-primed cDNA synthesis according to the reverse transcription protocol (TaKaRa). The resulting cDNA was subjected to relative quantitative PCR using the SYBR Premix Ex Taq kit (TaKaRa ...
-
bioRxiv - Neuroscience 2022Quote: ... Target sequences were amplified by 18–30 cycles of PCR using Ex-Taq™ (Takara Bio). For semiquantitative PCR ...
-
bioRxiv - Physiology 2023Quote: ... Slides were incubated for 18 hours at room temperature in anti-dsRed antibody (TaKaRa, 1:2000) in 0.1M PB containing 0.3% Triton X-100 and 1% donkey serum ...
-
bioRxiv - Microbiology 2021Quote: The 16S rRNA gene was amplified using LA Taq polymerase (TaKaRa-Bio, Inc.). For pyrosequencing ...
-
bioRxiv - Neuroscience 2023Quote: ... Tissue was incubated for 16 hr in rabbit anti-DsRed (Clontech; 1:5000) and mouse anti-TH antibodies (Immunostar ...
-
Phylogeny-metabolism dual-directed single-cell genomics for dissecting and mining ecosystem functionbioRxiv - Microbiology 2023Quote: ... the 16S rRNA targeted oligonucleotide probes GAM42a (Table S1, TAKARA Bio Inc., Japan) was applied to the identification of γ-Proteobacteria ...
-
bioRxiv - Evolutionary Biology 2019Quote: ... All PCR reactions were performed in 24 μl volumes with 13 μl ExTaq HS polymerase (Takara Bio Inc.), 1 μL each forward and reverse primers ...
-
bioRxiv - Neuroscience 2021Quote: ... Neuronal morphology of 14 DIV neurons transfected with the plasmid peGFP-N1 (Clontech, Mountain View, CA) was examined using high-resolution confocal digital images obtained from an Opera-Phenix microscope (Perkin-Elmer) ...
-
bioRxiv - Cancer Biology 2020Quote: ... phospho-specific mutant constitutively active NFATC4 17 was also cloned into the doxycycline-inducible Tet-One expression system (Clontech) to create an inducible and constitutive NFATC4 (IcNFATC4 ...
-
bioRxiv - Microbiology 2023Quote: ... HEK-293/17 cell media were harvested and concentrated overnight with a Lenti-X concentrator (Clontech, Mountain View, CA) according to the manufacturer’s protocol and resuspended in 10-fold less media volume prior to concentration.
-
bioRxiv - Molecular Biology 2023Quote: ... and then used as the template to synthesize double strand cDNA amplicons by PCR (optimal 17 – 21 cycles) using Advantage 2 PCR kit according to the manufacturer’s specifications (Clontech). cDNA was purified using NucleoSpin Gel and PCR Clean-up kit (Takara Bio) ...
-
bioRxiv - Cancer Biology 2024Quote: ... The 13 kb band was purified with gel purification kit (Takara Nucleospin® Gel and PCR Clean-up Midi). Eighty-two base pair single stranded oligos were designed according to the following structure:
-
bioRxiv - Plant Biology 2020Quote: ... The 5’-3’ junction sequence was amplified by PCR with cox1 specific primers Atcox1-5’(−176..-196) and Atcox1-3’(+17..+38) using Ex Taq Hot Start Version (Takara). The thermal cycling program consisted of initial 4 minute-denaturation at 95°C ...
-
bioRxiv - Genetics 2021Quote: ... The plasmid was linearized using AgeI and SalI restriction enzymes and inserts were cloned to the linearized plasmid in 17 reactions using the standard InFusion cloning (Clontech) protocol ...
-
bioRxiv - Molecular Biology 2023Quote: ... DNA encoding HsPINK1 was inserted into the BamHI site of pcDNA5/FRT/TO CMVd3 vector (pcDNA5d3, see (17)) using the In-Fusion HD Cloning Kit (Takara). For stable expression ...
-
bioRxiv - Developmental Biology 2023Quote: ... 18-20 μg of indicated plasmids were transfected in HEK293T cells using the CalPhos Mammalian Transfection Kit (Takara). After 48h ...
-
bioRxiv - Biochemistry 2022Quote: ... 9, 11, 13, 20, or 45 (SERF2, C9orf16, C19orf53, C11orf58, BEX3, or SERBP1 respectively) was inserted into pCold I (Takara), together with the client protein ...
-
bioRxiv - Molecular Biology 2022Quote: ... cDNA was amplified by PCR in separate 25 μL reactions each containing 2 μL of cDNA for 11-13 cycles using 0.5 μL (2.5 U) LA Taq (Takara, Cat# RR002M) with P5-3’ and miRCat-PCR-2 oligos at an annealing temperature of 58°C ...
-
bioRxiv - Neuroscience 2021Quote: ... coated T-225 flasks containing 85-95% confluent HEK-293T/17 cells (ATCC, CRL-11268) were each transfected (Xfect, Takara #631318) with a DNA cocktail containing the 1 ...