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Citations for Takara Bio :
101 - 150 of 5440 citations for Cow Casein Kinase II Subunit Alpha 2 CSNK2A2 ELISA Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Evolutionary Biology 2024Quote: ... using the Advantage® 2 PCR Kit (Takara Bio) in a touchdown cycling program as follows ...
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bioRxiv - Cell Biology 2021Quote: ... red fluorescent protein fused with the mitochondrial targeting sequence from cytochrome c oxidase subunit VIII (Clontech, Mountain View, CA) using Lipofectamine 2000 (Life Technologies ...
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bioRxiv - Cell Biology 2020Quote: ... Reverse transcription was performed using PrimeScriptTM II 1st strand cDNA Synthesis Kit (TaKaRa, Tokyo, Japan) with Oligo-T primer to obtain cDNA ...
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bioRxiv - Microbiology 2021Quote: ... Quantitative PCRs were performed using a SYBR PremixEX Taq II (RT)-PCR kit (Takara, Japan) on a Thermo PIKOREAL 96 real-time PCR System ...
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bioRxiv - Molecular Biology 2022Quote: ... and also remaining polymerase enzyme by use of Nucleospin® Extract II Kit (Clontech Laboratories) following Manufacturer’s protocol ...
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bioRxiv - Microbiology 2023Quote: ... cDNA was synthetized from total RNA using PrimeScript II 1st Strand cDNA Synthesis Kit (Takara) and amplified using ChamQ Universal SYBR qPCR Master Mix (Vazyme Biotech ...
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bioRxiv - Biochemistry 2021Quote: ... T4 polynucleotide kinase and TransIT®-LT1 Reagent were purchased from TAKARA (Shiga, Japan). SB203580 was purchased from Adipogen Life Science (San Diego ...
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Engineering of membrane complex sphingolipids improves osmotic tolerance of Saccharomyces cerevisiaebioRxiv - Bioengineering 2019Quote: Total RNA was extracted using MiniBEST universal RNA extraction kit and 1 μg was taken to synthesize cDNA using the PrimeScript II 1st-strand cDNA synthesis kit (TaKaRa, Japan). The cDNA mixture was diluted to about 100 ng/μl and used as the template for the gene expression level analysis by qRT-PCR ...
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bioRxiv - Microbiology 2022Quote: ... X-alpha-Gal (X-α-gal) and aureobasidin A (AbA) were obtained from TaKaRa. Plasmids ...
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bioRxiv - Molecular Biology 2021Quote: ... we performed ELISA against HIV p24 (TaKaRa Clontech #632200) according to manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2021Quote: ... we performed ELISA against HIV p24 (TaKaRa Clontech #632200) according to manufacturer’s instructions ...
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bioRxiv - Genomics 2024Quote: ... and titer quantified using p24 ELISA antigen assay (Takara). MOI=5 was used to transduce 1x1097 EndoC-βH3 cells in culture media without pen/strep and puromycin.
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bioRxiv - Neuroscience 2024Quote: ... HEK293 cells in 24-well plates were transfected with a total of 0.9 μg of DNA vectors carrying genes encoding GluN1 and GluN2 subunits and green fluorescent protein (GFP; pQBI 25 vector; for identification of transfected cells; Takara) in a 1:1:1 ratio using 0.9 μl of PolyMag reagent (OZBiosciences ...
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bioRxiv - Neuroscience 2021Quote: ... Reverse transcription for cDNA cloning was performed with PrimeScriptTM II 1st Strand cDNA Synthesis kit (Takara), Gibson assembly was performed with NEBuilder HiFi DNA
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bioRxiv - Physiology 2020Quote: ... cDNA was synthesized using a Takara PrimeScript™ II 1st strand cDNA Synthesis Kit (Takara, 6210). Real-time PCR was performed using probes from Applied Biosystems and Premix Ex Taq (Takara ...
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bioRxiv - Microbiology 2021Quote: Total RNA was reverse-transcribed to cDNA using the PrimeScript II Reverse transcriptase kit (Takara Bio) or the SuperPrep II Cell Lysis & RT kit for qPCR (TOYOBO ...
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bioRxiv - Neuroscience 2022Quote: ... and MEFs were reverse-transcribed using a PrimeScript II first-strand cDNA Synthesis Kit (Takara Bio) with a random hexamer primer ...
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bioRxiv - Immunology 2020Quote: ... followed by first-strand cDNA synthesis via PrimeScriptTM II 1st Strand cDNA Synthesis Kit (Takara, Japan) using miR-137- primer(5’-GTCGTATCCAGTGCAGGGTCCGAGGTCACTGGATACGACACGTG TAT-3’ ...
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bioRxiv - Microbiology 2021Quote: ... The PCR was performed with the One Step TB Green PrimeScript RT-PCR Kit II (TaKaRa), and SYBR Green was used as a fluorescent dye for detection ...
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bioRxiv - Molecular Biology 2022Quote: ... qPCR was performed using the SYBR Premix Ex Taq II Kit (TaKaRa Biotechnology, Dalian, Liaoning, China) on ABI 7500 Sequence Detection System software version 1.2.3 (Applied Biosystems ...
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bioRxiv - Cancer Biology 2022Quote: ... in-situ apoptosis detection kit and TB Green Premix Ex Taq II were procured from Takara Bio Inc ...
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bioRxiv - Biochemistry 2023Quote: ... and then reverse transcribed into cDNA using PrimeScript II 1st strand cDNA synthesis kit (Takara Bio). PCRs for AgApiT and FNS I (Yan et al ...
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bioRxiv - Microbiology 2024Quote: ... was reverse-transcribed using a PrimeScript II 1st strand cDNA Synthesis Kit (Takara Bio, Shiga, Japan) according to the manufacturer’s protocol ...
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bioRxiv - Immunology 2023Quote: ... and GC B cells (live singlet CD19+ CD4− IgDlo CD71+CD38int CD20+ CXCR5+) were sorted using a FACSAria II into 96-well plates containing 2 μL Lysis Buffer (Clontech) supplemented with 1 U μl−1 RNase inhibitor (NEB) ...
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bioRxiv - Neuroscience 2019Quote: ... Amplification was performed using Advantage GC 2 PCR kit (Clontech) and PCR products were cloned and sequenced.
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bioRxiv - Biochemistry 2022Quote: ... restriction enzymes and DNA ligation kit ver.2 (Takara Bio); pET21a and E ...
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bioRxiv - Cell Biology 2023Quote: ... and the 5′-end was phosphorylated with T4 polynucleotide kinase (Cat. # 2021S: Takara, Kyoto, Japan). This phosphorylated DNA fragment was ligated to the Bbs I cloning site in pSpCas9(BB)-2A-Puro (PX459 ...
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bioRxiv - Microbiology 2021Quote: ... real-time RT-PCR was performed with SARS-CoV-2 N primer sets and SYBR Premix Ex Taq II (TaKaRa-Bio) using a LightCycler Nano (Roche ...
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bioRxiv - Immunology 2021Quote: ... using One Step TB Green(tm) PrimeScript(tm) RT-PCR Kit II (SYBR Green) (RR086B, TaKaRa, JAPAN). Relative copy number was determined by calculating the fold-change difference in the gene of interest relative to GAPTH ...
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bioRxiv - Plant Biology 2020Quote: ... and first-strand cDNA was synthesized using the PrimeScript II 1st strand cDNA Synthesis Kit (TAKARA Bio), according to the manufacturer’s protocol.
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bioRxiv - Genetics 2021Quote: ... and One Step TB Green™ PrimeScript™ RT-PCR Kit II (SYBR Green) (TaKaRa RR086B, Japan).
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bioRxiv - Plant Biology 2020Quote: ... and reverse-transcribed with a random hexamer using the PrimeScript II 1st strand cDNA Synthesis kit (TaKaRa). Gene specific primers were used to amplify cDNA by PCR using Ex Taq Hot Start Version (Takara) ...
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bioRxiv - Plant Biology 2020Quote: ... First-strand cDNA was synthesized using the PrimeScriptTM II 1st Strand cDNA Synthesis Kit (TAKARA, Kusatsu, Japan) according to the manufacturer’s protocol ...
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bioRxiv - Microbiology 2020Quote: ... or (ii) the SMARTer Stranded Total RNA-Seq Kit v2 – Pico Input Mammalian libraries (Takara Bio USA) for ALG_1 and ALG_3 (Table S1) ...
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bioRxiv - Plant Biology 2021Quote: ... the RNA was converted into cDNA using PrimeScript™ II 1st Strand cDNA Synthesis Kit (TAKARA, 6210A) following the instruction in the manual ...
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bioRxiv - Plant Biology 2021Quote: ... Applied Biosystem StepOne real-time PCR system and SYBR Premix Ex Taq II kit (Takara, Dalian, China) were used for the qRT-PCR detection ...
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bioRxiv - Plant Biology 2021Quote: ... Reverse transcription of RNAs were carried out using PrimeScript II 1st strand cDNA Kit™ (Takara, Japan). The coding sequences of DGAT1s were amplified by a high-fidelity KOD-Plus-Neo polymerase (Toyobo ...
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bioRxiv - Molecular Biology 2019Quote: ... qRT-PCR reactions were performed using a SYBR® Premix Ex Taq™ II Kit (TaKaRa, China) and a CFX96 real-time PCR detection system (BIO-RAD ...
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bioRxiv - Molecular Biology 2019Quote: ... Quantitative PCR was performed using a One Step TB Green PrimeScript RT–PCR Kit II (Takara, #RR086A) on a LightCycler 2.0 instrument (Roche) ...
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bioRxiv - Microbiology 2021Quote: ... and MSN4 were measured by RT-PCR using One Step SYBR PrimeScript RT–PCR Kit II (TaKaRa). Reactions were run on Mx3005P qPCR System (Agilent Technologies ...
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bioRxiv - Developmental Biology 2020Quote: ... DNase treated RNA was used for cDNA synthesis through PrimeScript II 1st Strand cDNA Synthesis Kit (Takara) according to the manufacturer’s instructions ...
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bioRxiv - Microbiology 2020Quote: cDNAs were synthesized by reverse transcription using a PrimeScript II 1st Strand cDNA Synthesis Kit (Takara Bio). Total RNA (1 μg ...
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bioRxiv - Genetics 2022Quote: ... Reverse transcription for cDNA cloning was performed with PrimeScript™ II 1st Strand cDNA Synthesis kit (Takara), Gibson assembly was performed with NEBuilder HiFi DNA Assembly Master Mix ...
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bioRxiv - Molecular Biology 2022Quote: ... The DNA band was cut out and purified using the Nucleospin® Extract II Kit (Clontech Laboratories) protocol ...
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bioRxiv - Cell Biology 2023Quote: ... the first strand cDNA was prepared using PrimeScript II 1st Strand cDNA Synthesis Kit (Takara, Beijing, China). qPCR was performed using 2× SYBR Green qPCR Mix (Low ROX ...
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bioRxiv - Plant Biology 2021Quote: ... Yeastmaker™ Yeast Transformation System 2 kit (Clontech, Mountain View, USA) was used for the process of yeast transformation ...
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bioRxiv - Microbiology 2023Quote: ... corresponding to these sgRNA sequences (LpAsp_For and LpAsp_Rev as well as LpCht_For and LpCht_Rev) were phosphorylated by T4 polynucleotide kinase (TAKARA) followed by annealing and cloning into BbsI digested pSPneogRNAH vector (Zhang and Matlashewski ...
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bioRxiv - Systems Biology 2021Quote: ... We prepared ligation-free ribosome profiling and total RNA-seq libraries from the clarified polysome lysates treated for 6 hours following the instructions provided with their respective kits (smarter-seq smRNA-seq kit, Takara-Clontech; NEBnext Ultra-Directional II) augmented with our previously-published ligation-free ribosome profiling protocol42 ...
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bioRxiv - Cell Biology 2020Quote: ... and reverse transcribed into cDNA using reagents from a Prime Script II first strand cDNA synthesis kit (Takara). Real-time PCR was performed with the reverse transcription products ...
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bioRxiv - Microbiology 2021Quote: ... Viral RNA was amplified using PrimeScript II High Fidelity One Step RT-PCR Kit (Takara Bio, Shiga, Japan) under the following reaction conditions ...