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Citations for Takara Bio :
1 - 50 of 743 citations for Apolipoprotein B mRNA Editing Enzyme Catalytic Subunit 3G APOBEC3G Antibody since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2023Quote: The Ub(K48R)-IBM and Ub(K48R)ΔLRGG-IBM expression constructs were generated by gene synthesis and cloned to a Tet-on 3G inducible PiggyBac vector using In-Fusion HD enzyme (Clontech). The IgA2 and its IBM mutant constructs were cloned to a PCDNA3.1 vector under the control of a CMV promoter ...
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bioRxiv - Microbiology 2019Quote: ... the Tet-ON 3G expression system (Clontech) was used ...
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bioRxiv - Molecular Biology 2022Quote: ... HeLa Tet On 3G cells (TaKaRa Bio) were transfected with pTRE3G-BI-GFP-FLAG-p27 FL or pTRE3G-BI-GFP-FLAG-p27* ...
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bioRxiv - Molecular Biology 2020Quote: The Tet-On 3G Inducible Expression System (Clontech) was used according to the manufacturer’s instructions to establish Tet-On 3G CARD14 inducible cell lines ...
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LIR-dependent LMX1A/LMX1B autophagy crosstalk shapes human midbrain dopaminergic neuronal resiliencebioRxiv - Cell Biology 2019Quote: ... either the Tet-On 3G inducible system (Clontech), LMX1B-FLAG in pcDNA3.1 (Genscript) ...
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bioRxiv - Cell Biology 2021Quote: ... HA-APOBEC3G was cloned using cDNA from TZMbl cells in pCMV-HA vector from Clontech, USA ...
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bioRxiv - Immunology 2019Quote: ... HEK293T cells were transfected with either the Tre-3G-Ptpn2 or Tet-On 3G constructs using the Lenti-X Packing system (Clontech Laboratories) according to the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2022Quote: ... pLVX TRE 3G and pLVX EF1Į Tet3G were part of Tet-On® 3G Inducible Expression Systems (631363, Takara Bio Inc.). pCMV6-Entry Myc DDK CD147 (BSG ...
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bioRxiv - Developmental Biology 2021Quote: ... Tet-ON 3G doxycycline (Dox)-inducible lentiviral vector (Takara, 631337) was modified by inserting EF1a-TagRFP-2A-tet3G with tetON-NKX2-1 CDS ...
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bioRxiv - Cell Biology 2021Quote: Inducible gene expression system based on Tet-On 3G (Clontech) was employed ...
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bioRxiv - Cell Biology 2023Quote: ... and NIH/3T3 Tet-On 3G (Takara Bio, Kusatsu, Japan) cells were cultured in DMEM ...
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bioRxiv - Cell Biology 2019Quote: HEK 293 Tet-On® 3G cells were purchased from Clontech and cultured in Minimum Essential Medium (MEM ...
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bioRxiv - Synthetic Biology 2020Quote: ... 100ng of pCMV Tet3G from the Tet-on 3G system (Clontech, 631168), and 100ng of plasmids 116v1 ...
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bioRxiv - Cell Biology 2023Quote: ... followed by the reverse tTA (rtTA) gene (Tet-On 3G, Takara Bio), separated via self-cleaving T2A peptide was generated via Gibson Assembly (NEB) ...
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bioRxiv - Molecular Biology 2023Quote: ... Monoclonal U-2OS cell lines stably expressing Tet-On 3G transactivator protein (Clontech), a tandem-dimeric MS2 hairpin binding protein tagged with eYFP (MS2CP-YFP) ...
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bioRxiv - Cancer Biology 2023Quote: ... and either 2 μg of the Tet-On-3G transactivator (pLVX Tet3G, Clontech) or 2 μg of the pLenti-CMVtight-Hygro-DEST plasmid with each cDNA of interest ...
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bioRxiv - Cancer Biology 2023Quote: ... mRNA was utilized with the SMARTer Human TCR a/b Profiling Kit v2 (Takara, USA). The resulting libraries were sequenced for paired-end reads of 2×250 bp on an Illumina system at a sequencing depth of 7.5X.
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bioRxiv - Genetics 2021Quote: ... the lentiviral Lenti-X™ Tet-On® 3G Inducible Expression System (Takara Bio) was used ...
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The spindle protein CKAP2 regulates microtubule dynamics and ensures faithful chromosome segregationbioRxiv - Cell Biology 2023Quote: ... the Tet-On® 3G Inducible Expression System kit was used (Takara Bio; 631167). RPE-1 wild-type and CKAP2-KO cells were transfected with a pEF1α-TET3G plasmid for expression of a Tet-On 3G transactivator protein ...
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bioRxiv - Genomics 2020Quote: ... CDT1 cDNA was further cloned into the Tet-On 3G inducible expression system (TaKaRa, 631168) with a flag tag at the N terminal and an EGFP tag at the C terminal by the In-Fusion HD Cloning system (TaKaRa ...
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bioRxiv - Developmental Biology 2022Quote: ... a lentiviral approach was developed using the Lenti-X Tet-On 3G Inducible Expression System (Clontech). The ORF encoding Fgfr2iiib (Origene ...
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bioRxiv - Developmental Biology 2023Quote: ... This rtTA-advanced ORF was replaced with rtTA3G from the pCMV-Tet-3G plasmid (TaKaRa Bio), by XbaI and XmaI digestion and cloning of three separate fragments to create the final rtTA vector AAVS1-NEO-UBrtTA-3G.
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bioRxiv - Biochemistry 2022Quote: ... The pTRE3G vector containing PFK1-mEGFP was then co-transfected into HeLa Tet-On 3G cells (Clontech) along with a linear selection marker of hygromycin using Xfect (Clontech) ...
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bioRxiv - Developmental Biology 2020Quote: ... and catalytic core (residues 105–411) of SlPP2C3 were cloned into Y2H ‘prey’ vector pGADT7 (Clontech). Full-length SlPYLs and SlSnRK2s were cloned into the ‘bait’ vector pGBKT7 (Clontech) ...
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bioRxiv - Molecular Biology 2023Quote: ... ΔHEL2i and ΔHEL2 DICER and their catalytic mutants (CM) were generated by InFusion mutagenesis (Takara Bio) on pDONR-DICER and pDONR-DICER catalytic mutant vectors ...
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bioRxiv - Developmental Biology 2023Quote: ... TRE3Gs and Tet-ON 3G were amplified by PCR from AAVpro Tet-One Luc Control Vector (Clontech, Cat# 634311), Kaede from in-house recombineering cassette (Zheng et al. ...
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bioRxiv - Developmental Biology 2021Quote: ... AP20187 (B/B) compound (Takara Bioscience) was injected into the dorsal aorta through a small window made in the ventral egg ...
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bioRxiv - Biophysics 2022Quote: ... B/B homodimeriser (AP20187, Takara Bio), dibenzocyclooctyne-PEG4-maleimide (760676 ...
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bioRxiv - Molecular Biology 2022Quote: ... Each sample was then incubated at 37°C for 30 min with 20 U of mRNA-Interferase™ MazF enzyme (Takara, Cat# 2415A) in a 10 μL volume reaction containing 1X MazF reaction buffer ...
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bioRxiv - Developmental Biology 2020Quote: Doxycycline-inducible overexpression of HA-tagged proteins was achieved using the Lenti-X Tet-On 3G Inducible Expression System (Clontech) following the manufacturer’s protocol ...
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bioRxiv - Genomics 2019Quote: ... The Human EF1a Promoter/Tet-On 3G fragment as well as the Neo(R) cDNA were PCR amplified from the pEF1α-Tet3G (Cat. # 631336, Clontech) using the following primer pairs
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bioRxiv - Neuroscience 2023Quote: ... pTRE3G-HA signal-Flag-GPCRs-P2A-mCherry-reverse-PGK-TetOn3G was made of Tet-ON 3G inducible expression system (Clontech) and PRESTO-Tango GPCR Kit (Kroeze et al. ...
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bioRxiv - Cancer Biology 2022Quote: Doxycycline inducible RARα expressing phyllodes tumor patient-derived cells (PT024 RARα) were established with Tet-On 3G system from Takara. Coding sequence of full-length WT RARα were cloned into a PLVX-Tre3G-IRES-GFP response vector ...
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bioRxiv - Synthetic Biology 2022Quote: ... B/B dimerizer (AP20187 ligand; TaKaRa 635058) was added to a final concentration of 500 nM in the well ...
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bioRxiv - Immunology 2022Quote: ... B/B homodimerizer (Takara Bio, Cat#635058), b-estradiol (Sigma-Aldrich ...
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bioRxiv - Cell Biology 2022Quote: ... 500 nM B/B Homodimerizer (Takara, #635058) was also added ...
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bioRxiv - Neuroscience 2023Quote: B/B homodimerizer was purchased from Takara USA lnc ...
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bioRxiv - Molecular Biology 2021Quote: ... mRNA was prepared using the NucleoTrap mRNA Mini Kit (Clontech) from 500 ng of total RNA ...
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bioRxiv - Immunology 2019Quote: ... conjugated to vinculin or the vinculin A50I mutant were cloned into the doxycycline-inducible Tet-On 3G plasmid system (Takara Bio). HoxB8-conditional progenitors (expressing Cas9 and control sgRNA or Vcl (2 ...
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bioRxiv - Biophysics 2021Quote: ... The rTetR protein sequence was subcloned from the Tet-On transactivator used in the commercially available Tet-On 3G system (Takara Bio). FUSN was derived from Addgene #122148 and GBP was based on a previously described construct (Rothbauer et al. ...
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bioRxiv - Biochemistry 2020Quote: Induced expression of the bidirectional plasmid required the use of HEK 293 Tet-On® 3G cells (Takara Clontech Cat# 631182). Cells in 6 well plates were transfected with 1 μg of the bidirectional plasmid using lipofectamine 3000 (Thermo Fisher Scientific Cat# L3000-008 ...
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bioRxiv - Biochemistry 2020Quote: Induced expression of the bidirectional plasmid required the use of HEK 293 Tet-On® 3G cells (Takara Clontech Cat# 631182). Cells in 6 well plates were transfected with 1 μg of the bidirectional plasmid using lipofectamine 3000 (Thermo Fisher Scientific Cat# L3000-008 ...
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bioRxiv - Biochemistry 2022Quote: A HeLa-T-PFK1-mEGFP stable cell line (hereafter, HeLa-T-PFK1G) was generated using the HeLa Tet-On® 3G inducible expression system (Clontech) according to the manufacturer’s protocol ...
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bioRxiv - Molecular Biology 2020Quote: ... we first produced lentivirus with Pup (E) plasmid Bio-Pup (E)-IRES-BFP within the Tet-On 3G inducible expression system (Clontech 631168), and infected OVCAR-5 cells for 48 hours ...
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bioRxiv - Immunology 2019Quote: ... murine Ptpn2 cDNA from HER-2-E0771 cells was reverse transcribed and amplified by PCR and cloned into the SmaI and EcoR1 restriction sites of Tre-3G plasmid (Clontech Laboratories) to generate the Tre-3G-Ptpn2 construct ...
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bioRxiv - Cell Biology 2021Quote: ... we first produced lentivirus with Pup(E) plasmid Biotin-Pup(E)-IRES-BFP within the Tet-On 3G inducible expression system (Clontech 631168), and infected Jurkat cells for 48 hours ...
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bioRxiv - Immunology 2022Quote: Doxycycline (Dox)-inducible gene-expressing cell lines were generated using Retro-X™ Tet-On 3G Inducible Expression System (Takara Bio). Plat-GP cells were transfected with the pRetroX-Tet3G plasmid together with the packaging plasmid pCMV-VSV-G using Lipofectamine 2000 ...
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bioRxiv - Pathology 2020Quote: AP21087 ((B/B homodimerizer, Takara Bio, CA, USA) was administered by intra-peritoneal (ip ...
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bioRxiv - Cell Biology 2019Quote: ... AP20187 (B/B Homodimerizer) was purchased from Takara. The recombinant Anti-M1 Spastin rabbit monoclonal antibody ...
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bioRxiv - Synthetic Biology 2022Quote: ... 500 nM of the B/B dimerizer (TaKaRa 635058) was added 2 hours before the start of the assay (or an Ethanol vehicle control was added) ...