Labshake search
Citations for Takara Bio :
201 - 250 of 1098 citations for Anti Flag Affinity Gel since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Biochemistry 2022Quote: ... The supernatant was subjected to immobilized metal affinity chromatography (IMAC) with talon resin (Takara Bio USA). The bound protein was washed with TBS buffer containing 0.02% GDN and 20 mM imidazole and eluted with TBS buffer containing 0.02% GDN and 250 mM imidazole ...
-
bioRxiv - Microbiology 2022Quote: ... The supernatants containing soluble target proteins were loaded into a Talon metal affinity resin (Clontech, USA). After washing three times with buffer A ...
-
bioRxiv - Immunology 2022Quote: ... SARS-CoV-2 RBDs were purified using a Cobalt affinity column (HisTALON Superflow column from Takara or HiTrap TALON crude column from Cytiva ...
-
bioRxiv - Cell Biology 2023Quote: ... His-tagged recombinant proteins were isolated by 1 h incubation with Talon metal affinity resin (Clontech) at room temperature ...
-
bioRxiv - Biochemistry 2022Quote: ... Scaffolding protein was purified on a TALON Superflow metal affinity column (Takara Bio, San Jose, CA). Fractions containing scaffolding protein were pooled and concentrated in 12-14kDa MWCO dialysis tubing (Spectrum Chemical ...
-
bioRxiv - Immunology 2023Quote: ... harvested supernatants were purified using a Talon NTA-cobalt affinity column (Takara Bio, Kusatsu, Shiga, Japan) with elution in 200 mM Imidazole ...
-
bioRxiv - Molecular Biology 2020Quote: ... Samples were analyzed on bleach agarose gels (0.06% bleach, 1% (w/v) agarose) for visualization of the RNA and on WB (anti-His antibody, Clontech) for visualization of His6-tagged Nsp1.
-
bioRxiv - Molecular Biology 2019Quote: ... and were then gel-eluted using NucleoSpin® Gel and PCR Clean-up (740609.10; Clontech). One amplicon encodes the first 34 amino acids of REH2 (primers ...
-
bioRxiv - Molecular Biology 2021Quote: ... The supernatant was then mixed with 50 µL cobalt beads (TALON® Metal Affinity beads Clontech, 635502) prewashed three times in guanidium hydrochloride buffer and the samples incubated at RT for 3 h with rotation ...
-
bioRxiv - Cell Biology 2019Quote: ... The supernatant was pH’ed to 7.6 and mixed with 1 ml Talon Metal affinity resin (Clontech 635509) for 30 min ...
-
bioRxiv - Microbiology 2019Quote: ... and the supernatant was applied to 1 mL of pre-washed TALON metal affinity resin (Takara Clontech) at 4°C ...
-
bioRxiv - Microbiology 2019Quote: ... and the supernatant was applied to 1 mL of pre-washed TALON metal affinity resin (Takara Clontech) at 4°C ...
-
bioRxiv - Biochemistry 2022Quote: ... aIF1A was purified as described (24) except that a standard affinity chromatography step on Talon resin (Clontech) was added to the original protocol ...
-
bioRxiv - Biochemistry 2021Quote: ... After 12 h medium was collected and recombinant proteins were purified on TALON metal affinity resin (Takara). 200 μl of the resin was loaded onto 1 ml column (Bio-Rad ...
-
bioRxiv - Bioengineering 2019Quote: ... The supernatant was transferred into a new tube with 400 μl of Talon metal-affinity resin (Clontech), equilibrated with phosphate buffer ...
-
bioRxiv - Cell Biology 2020Quote: ... coli cells and purified to near-homogeneity by metal-affinity chromatography using TALON resin (Takara Bio, USA). After purification ...
-
bioRxiv - Immunology 2021Quote: ... Supernatant from Expi293 transfected cells was manually purified using TALON metal affinity chromatography (Takara bio Inc – 635502), according to manufacturer’s recommendations ...
-
bioRxiv - Molecular Biology 2022Quote: ... Cleared lysate was bound to 3 mL (=1.5 mL bed volume) Talon SuperFlow Metal Affinity Resin (TaKaRa) per protein preparation ...
-
bioRxiv - Immunology 2022Quote: ... Supernatants were collected and purified using a Talon NTA-cobalt affinity column (Takara Bio, Kusatsu, Shiga, Japan) with elution in 200 mM Imidazole ...
-
bioRxiv - Microbiology 2022Quote: ... The concentrated supernatant was passed through an equilibrated TALON Metal Affinity Resin (Takara Bio Inc, Shiga, Japan). The column was washed with 10 volume of PBS containing NaCl (300 mM ...
-
bioRxiv - Biochemistry 2023Quote: ... The supernatant was incubated overnight with 0.5 ml of Talon (immobilised metal affinity chromatography, IMAC) resin (Clontech) in the presence of 150 mM NaCl and 20 mM imidazole ...
-
bioRxiv - Cell Biology 2023Quote: ... The supernatant was collected and allowed to bind with TALON® Metal Affinity Resin (Clontech Laboratories #635503) for 1 hour at RT with rotation ...
-
bioRxiv - Biochemistry 2024Quote: ... Lysate was filtered using a 0.25 µm syringe filter and applied to Talon metal affinity resin (Takara) in 50mM Tris-HCl ...
-
bioRxiv - Biochemistry 2023Quote: ... and hSTEAP2 was purified from the supernatant using cobalt-based Talon affinity resin (Clontech, Mountain View, CA). The C-terminal His6-tag was cleaved with tobacco etch virus (TEV ...
-
bioRxiv - Bioengineering 2024Quote: ... All proteins were purified with a Talon metal affinity resin (Takara Bio USA, Mountain View, CA, USA) and dialyzed against pyrogen-free PBS ...
-
bioRxiv - Molecular Biology 2023Quote: ... gel purified using the Nucleospin Gel and PCR Clean-Up Kit (TaKaRa Bio Inc., Kusatsu, Japan), and then cloned into the XbaI site of pHIV7/PGK-neo using InFusion cloning (TaKaRa Bio Inc. ...
-
bioRxiv - Biophysics 2022Quote: ... The stability of the fusion constructs was verified by gel electrophoresis and immunoblotting using an anti-GFP primary antibody (JL-8 monoclonal, Takara). Point mutations were introduced by site-directed mutagenesis (New England Biosciences) ...
-
bioRxiv - Genetics 2020Quote: Myomixer-FLAG was packaged in the pLVX-TetON (Takara Bio; Mountain View, CA) plasmid and have been described in previous literature64 ...
-
bioRxiv - Cell Biology 2019Quote: FLAG-tag-hFAIM-S expression vector was constructed using pCMV-(DYKDDDDK)-C (Takara) (cloned into EcoRⅠ and KpnⅠ sites) ...
-
bioRxiv - Immunology 2021Quote: ... Protein eluted from the Strep-Tactin column was then applied to pre-equilibrated TALON Metal Affinity Resin (Takara) and allowed to enter the column by gravity flow ...
-
bioRxiv - Biochemistry 2020Quote: ... Recombinant protein was then purified by immobilized metal ion affinity chromatography using a cobalt-based matrix (Talon, Clontech) and eluted with 100 mM imidazole ...
-
bioRxiv - Immunology 2021Quote: ... secreted protein was purified from the culture supernatant 4 days post transfection using TALON metal affinity resin (Clontech) and Amicon Ultra 10K filter device (Millipore) ...
-
bioRxiv - Biochemistry 2021Quote: ... Recombinant protein was then purified by immobilized metal ion affinity chromatography using a cobalt-based matrix (Talon, Clontech) and eluted with 100 mM imidazole ...
-
bioRxiv - Plant Biology 2022Quote: ... Purification of His-tagged proteins was carried out using His-tag affinity resin (His-resin) (Clontech, CA, USA).
-
bioRxiv - Immunology 2020Quote: ... The clarified extract was loaded onto a column containing TALON Metal Affinity Resin (BD Clontech, Mountain View, CA) according to the manufacturer’s instructions ...
-
bioRxiv - Plant Biology 2023Quote: ... The supernatant was applied to a TALON® metal affinity resin column (Takara Bio, Mountain View, CA, USA), and the column was washed with 10 column volumes of wash buffer (50 mM Tris-HCl ...
-
bioRxiv - Microbiology 2024Quote: ... and the protein was purified using cobalt affinity resin (1mL/L culture, Takara Bio USA, San Jose, CA). The protein was washed with 100 mM NaCl ...
-
bioRxiv - Microbiology 2024Quote: ... The supernatants from the samples were added to 0.5 ml of a Talon metal affinity resin (Clontech, USA) equilibrated with binding buffer ...
-
bioRxiv - Developmental Biology 2019Quote: ... The PCR products were extracted from agarose gel using MiniBEST Agarose Gel DNA Extraction Kit (Takara, Japan) based on manufacturer’s instructions ...
-
bioRxiv - Neuroscience 2023Quote: ... This band was cut from the gel and digested using the Takara Gel Purification kit (Takara #740609.50).
-
bioRxiv - Cell Biology 2022Quote: ... PCR products were then isolated through gel electrophoresis and purified using the Nucleospin gel purification kit (Takara Bio). Linear DNA fragments were then ligated using inFusion assembly and amplified in Stellar competent E ...
-
bioRxiv - Cancer Biology 2022Quote: ... The PCR products were purified from agarose gels using NucleoSpin Gel and PCR Clean-Up Kit (740609, Clontech) and subcloned into the pENTR/D-TOPO vector (K240020 ...
-
bioRxiv - Molecular Biology 2023Quote: ... The PCR product was gel purified using the Nucleospin Gel and PCR Clean-Up Kit (TaKaRa Bio Inc.) and cloned in between the XhoI and SalI sites of psiCHECK-2.2 using InFusion cloning (TaKaRa Bio Inc.) ...
-
bioRxiv - Developmental Biology 2023Quote: ... PCR products were cut from agarose gels and purified using the Nucleospin gel purification kit (Takara Bio, 240609). Plasmids were assembled from linear fragments using In-Fusion HD (Takara Bio ...
-
bioRxiv - Developmental Biology 2023Quote: ... PCR products were cut from agarose gels and purified using the Nucleospin gel purification kit (Takara Bio, 240609). Final plasmids were constructed using In-Fusion HD (Takara Bio ...
-
bioRxiv - Immunology 2023Quote: ... linearized BF520 sequence was gel purified using NucleoSpin Gel and PCR Clean-up kit (Takara, Cat. No. 740609.5) and then purified using Ampure XP beads (Beckman Coulter ...
-
bioRxiv - Microbiology 2024Quote: ... linearized GPC sequence was gel purified using NucleoSpin Gel and PCR Clean-up kit (Takara, Cat. No. 740609.5) and then purified using Ampure XP beads (Beckman Coulter ...
-
bioRxiv - Immunology 2021Quote: ... supernatants were harvested by centrifugation and further purified using immobilized metal affinity chromatography (IMAC) with cobalt-TALON resin (Takara) followed by Superdex 200 Increase 10/300 GL size exclusion column (GE Healthcare).
-
bioRxiv - Neuroscience 2020Quote: ... before addition of 4 mM imidazole and incubation on a rotor (111 RPM) with TALON metal affinity resin (Takara, Cat.# 635503 ...
-
bioRxiv - Biochemistry 2021Quote: ... The cell lysate was centrifuged at 40,000 × g for 45 min at 4 °C and the supernatant was loaded onto a cobalt affinity column (Clontech). After washing the column with 20 bed volume of 20 mM HEPES pH 7.5 ...